LOCUS       NZ_CP058964             4232 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain ZZW20 plasmid pZZW20-4.2K, complete
            sequence.
ACCESSION   NZ_CP058964
VERSION     NZ_CP058964.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15511588
            Assembly: GCF_013423825.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Bai,S.C., Li,R.B., Yang-Yu and Liao,X.P.
  TITLE     Sporadic dissemination of mcr-8-ST11 Klebsiella pneumoniae isolates
            in China
  JOURNAL   Enferm Infecc Microbiol Clin (Engl Ed) (2021) In press
   PUBMED   33632538
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Bai,S.-C. and Liao,X.-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2020) College of Veterinary Medicine, South China
            Agricultural University, Wushan Street No. 483, Guangzhou, Guangdong
            510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 03:57:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,778
            CDSs (total)                      :: 5,654
            Genes (coding)                    :: 5,546
            CDSs (with protein)               :: 5,546
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 43 of 108
            Pseudo Genes (incomplete)         :: 61 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 11 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058964.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ZZW20"
                     /isolation_source="sewage"
                     /db_xref="taxon:573"
                     /plasmid="pZZW20-4.2K"
                     /geo_loc_name="China:Fujian"
                     /lat_lon="23.34 N 116.54 E"
                     /collection_date="2018"
                     /collected_by="Shuan-cheng Bai"
     gene            join(4196..4232,1..77)
                     /locus_tag="H0E90_RS28670"
                     /old_locus_tag="H0E90_28670"
     CDS             join(4196..4232,1..77)
                     /locus_tag="H0E90_RS28670"
                     /old_locus_tag="H0E90_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_074184130.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDNAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(280..1005)
                     /locus_tag="H0E90_RS28675"
                     /old_locus_tag="H0E90_28675"
     CDS             complement(280..1005)
                     /locus_tag="H0E90_RS28675"
                     /old_locus_tag="H0E90_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_074184131.1"
                     /translation="MASMETEQQPQKNIEQIEVKAKRVQCFIDGYNLYHAIEETGNKAL
                     HWVDLKSLCKRYLPKDASIENVFWFSAKPSHLSRTVNDNYQNYTNALTECGVDVIGGRF
                     KRKNVKCEADNGCGEVFHKHEEKESDVNLAISLVSGACNNEYDIAIIITADSDLCPPIK
                     YVKERFPDKELWLVAPPGRIKRAADLCLMSSKNMEINSNALRASCMRDEFYKGGVLVAR
                     RPQEWRAIANTGQRRTRSR"
                     /name="NYN domain-containing protein"
     gene            1324..1935
                     /locus_tag="H0E90_RS28680"
                     /old_locus_tag="H0E90_28680"
     CDS             1324..1935
                     /locus_tag="H0E90_RS28680"
                     /old_locus_tag="H0E90_28680"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_074184132.1"
                     /translation="MSTENAQKEQKKESLITTHWGEDATLHGWTAVPNSLLMLQGDLGI
                     GSTEMCILLNVLMHQWPESGESISFPSIGTIASRMGVSKRTIQRGVSNLESLGILTRHQ
                     STRNDPRTNGANIFDSTPLKEHLNKKSKGITISRNKKKERKNIGTISNIKLPRLCPKCL
                     KTKATSYEEIVSFFGIRKTMAGEVINSYCKECRASEKNIF"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2074..2361)
                     /locus_tag="H0E90_RS28685"
                     /old_locus_tag="H0E90_28685"
     CDS             complement(2074..2361)
                     /locus_tag="H0E90_RS28685"
                     /old_locus_tag="H0E90_28685"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074184133.1"
                     /translation="MSDDSSVNLVPVTVFESQVEAVEGITIVVRAPSNTMVPEYTYVRR
                     SPGDNTVGEWMNNRINPSLNGLEVTIIDGEHESPRRSTKRLSTLRDSYER"
                     /name="hypothetical protein"
     gene            complement(3189..3590)
                     /locus_tag="H0E90_RS28690"
                     /old_locus_tag="H0E90_28690"
     CDS             complement(3189..3590)
                     /locus_tag="H0E90_RS28690"
                     /old_locus_tag="H0E90_28690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_024623230.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3590..3781)
                     /locus_tag="H0E90_RS28695"
                     /old_locus_tag="H0E90_28695"
     CDS             complement(3590..3781)
                     /locus_tag="H0E90_RS28695"
                     /old_locus_tag="H0E90_28695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          3902..4229
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.424"
                     /coverage="99.0"
                     /evalue="8.11e-130"
ORIGIN
        1 tatccggtaa ctatcgactt gagtccaacc cggaaagaca cgacaacgcg ccactggcag
       61 cagccactgg taactgattt agaggaggtg gtcttgaagt catgcgccta ttaaggctaa
      121 actgaaagaa caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
      181 cgccaactct atgaaccttc gaaaaaccgc ccgccaaggc ggttttttcg tttcaaccta
      241 gctagattaa tctccttact ctagctacgt caacaaagcc taacgacttc gagttcttct
      301 ttgcccggta ttggcaatag ctcgccattc ctgaggcctt ctggctacaa gcactccgcc
      361 tttgtagaac tcatcacgca tacagctagc tctcaaagca ttactgttga tctccatatt
      421 tttcgatgac ataaggcaga gatctgccgc tcgcttgatt cgccccggtg gagctacaag
      481 ccaaagttct ttatcaggga atcgctcttt tacgtattta ataggagggc ataagtcaga
      541 atccgcagtg ataataatgg ctatatcata ctcattatta caagcaccag acactaggct
      601 tatagccaaa ttaacatcgg attctttttc ttcatgctta tggaaaacct caccacatcc
      661 attatcagct tcgcacttta cattttttct tttaaaacgc ccaccgataa catcaacacc
      721 acattcagtt aaagcattcg tgtaattttg atagttatca tttaccgttc tcgataagtg
      781 tgatggcttg gcagaaaacc aaaaaacatt ctctatcgat gcatctttag gaaggtatct
      841 tttacataaa gatttaaggt caacccaatg aagagcttta ttacccgttt cttcaatagc
      901 atgatataaa ttatagccat caatgaaaca ctgaactcgc tttgctttta cttcgatttg
      961 ctcaatattt ttctgtggtt gctgctctgt ttccatactt gccatatttg acctctagat
     1021 agcaaaaagg cctgcatatg caggcctttt tgcgtctctt aaccgcagtt aacagaccac
     1081 tgtatggacg catccgatac aggaagaatt gtgtacaacg cgcttccaag cctcgacctg
     1141 gatagccact aagagcagaa ctcttaggta gtgaactcaa actaatttct atataaaaaa
     1201 agagcaaatg gatgacatat atgtcatcct ataaagatac atgttactac ctaactactc
     1261 tttataagag tagttaggta gtaactgttc acgttttgat tttttttcac gagaaaagca
     1321 cccatgagta cagaaaacgc acagaaagaa caaaaaaaag agtcattaat cactacgcac
     1381 tggggagagg atgcaactct acatgggtgg acagctgtac caaacagcct ccttatgctg
     1441 caaggcgatc ttgggattgg gagtactgag atgtgtattc tactgaatgt attaatgcat
     1501 caatggcctg aatcagggga gtcaatatct tttccttcaa ttggtaccat tgcatcaaga
     1561 atgggtgtat ctaaaagaac gattcaacgt ggagttagca atctagaatc attaggaata
     1621 ttgaccagac accaatcaac aaggaatgac cctagaacca atggggcaaa tatattcgat
     1681 tcaactccac taaaagaaca tttaaataaa aaatctaaag gtataacaat tagcagaaac
     1741 aaaaagaaag aaagaaagaa catcggaacc atatccaata taaaattacc tcgactttgc
     1801 cctaaatgtt taaaaactaa agcaaccagt tacgaagaga tcgttagttt ttttggcatc
     1861 cgaaagacaa tggctgggga agtaataaat tcatattgca aagaatgtcg agcttctgaa
     1921 aaaaacatat tttaaatatt aaaacatata aatatatggg aaaaggtgca gagtccaatt
     1981 aaaactttgc cccatttacc ataacgcctc attgcccgta ccacataaag aagccgctgt
     2041 ataagcggct tctcttattc atggtgatag cggttatcgc tcgtaactat cccgtaaagt
     2101 agataggcgc ttagttgaac gacgaggcga ttcatgctcc ccatcaataa tcgtgacttc
     2161 aaggccattt aatgacgggt taattctatt gttcatccac tctcctacgg tgttatcgcc
     2221 aggggatcgt ctaacatacg tatattcagg aaccatagta ttcgatggtg ctctaaccac
     2281 gatggtaata ccctcaaccg cttcaacctg cgactcaaaa accgtcacag gaactaagtt
     2341 aacactggaa tcatcagaca ttatattgac cttaccaagc aattaaattc tagttagcag
     2401 gaagttagcg agtaacccaa caaatgtcta ctgaaaattt caaaatgtac gatttaccat
     2461 aacgtcccat tacgcgcacc gctcaaacgc tccagaaacg attctggggc gtaacccatt
     2521 aaatcctacg tcttaccccc tgaaaacgcc tcaaaatcgc gctgatgggt tttacgggca
     2581 tgaattgtta tgcaccagac tggctggagg tagatttcat cctgaattca cgccagacaa
     2641 ctcaaaaccc gcaccaaacc cgactgaaaa cggtgaacgg ggacgcaatt tattgcgtgg
     2701 ccgtgaagcg ttgccggcgc agccggaaaa ccggttttca gcacgttcag gaatcgtgtt
     2761 cgtcgacggc cggcaatcac gttatggcaa gtcaccgaac gactcaccac acgattcacc
     2821 ggcgactctt cactagagtc gtacccagag tcgccacgtc taaaaaaccc ccaaacgggg
     2881 agtcgtgtct agagtcgggt cgttattaac tcattgtttt tatagtcctg agtcgttacc
     2941 gactctatga cgactcgccg gggactctat gacgagtcgt catagagtcg gatctatata
     3001 aatcaaattt aaaaaacgaa atttcaccaa cttaagctac tgatattaaa tacaaatccg
     3061 caaaatgtta attttgcgtg gggtgtgggt atttttagtg gtgaggagcg ttcagagatc
     3121 tgacgatttt tgggaaggtt caggaactcc ccgggatggc cggagaggaa aactgaagca
     3181 tgacggggtc aggatttttt ccagaatttc caccatggcg ccggggattc agttaatttt
     3241 gcctggtgat tttcaagaag gagcatggcc tgtttcaggc tgtcaatgtg cgaatccttg
     3301 gacaaaagta aggtgttcag gtgttcaatc tcctgtttca gatgacgaat cacctgttca
     3361 ctctggtcag cagaggctgt atcacgctgt tcagagacct gtcctgaatg gtgttcaccc
     3421 ttcagttgtt caaagggacc aaagacacgt aaaagctctg aagtatctac catttttgca
     3481 ttgcgttcac cgctacaggt ggacaggtca cctgctttaa ttaacctgtg cagtgttgtt
     3541 ctactcttac ccgtcagtcg ggcagcctca ctaatagaca ctctggccat cagttaccat
     3601 cctgtaatgc atccagtctc gaggacagtg tacggaaaag atgctccgca tcgtcgtgga
     3661 gtttctcaca aaggtcggcc tcagcatcaa gatcaagctc gttaagcttc tccagcagga
     3721 gcagagactg attctggatg aatttagcca tgttcagcgc tgttttctgt tgtttcgtca
     3781 taaggagcct cagagcgtca gtgctcaatg tagggatgtt ctcttttccg cttcctcgct
     3841 cactcagtcg ctacgctcct gccgttcggc tgcggcgagc ggcaggagca tgcgtataaa
     3901 gctcgagaaa accgaacggt cgcgtgatgg gaaaaggaaa ggccgcgcag cggccgtttt
     3961 tccataggct ccgcccccct gacaagcatc acagaaaaca gacgctcaaa tcagtggtgg
     4021 cgaaacccga caggactata aagataccta tcatttctga taggtggctc cctcgtgcgc
     4081 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
     4141 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
     4201 cgaacccccc gttcagtccg actgctgcgc ct
//
