LOCUS       NZ_CP058963             4436 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain ZZW20 plasmid pZZW20-4.4K, complete
            sequence.
ACCESSION   NZ_CP058963
VERSION     NZ_CP058963.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15511588
            Assembly: GCF_013423825.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Bai,S.C., Li,R.B., Yang-Yu and Liao,X.P.
  TITLE     Sporadic dissemination of mcr-8-ST11 Klebsiella pneumoniae isolates
            in China
  JOURNAL   Enferm Infecc Microbiol Clin (Engl Ed) (2021) In press
   PUBMED   33632538
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Bai,S.-C. and Liao,X.-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2020) College of Veterinary Medicine, South China
            Agricultural University, Wushan Street No. 483, Guangzhou, Guangdong
            510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 03:57:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,778
            CDSs (total)                      :: 5,654
            Genes (coding)                    :: 5,546
            CDSs (with protein)               :: 5,546
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 43 of 108
            Pseudo Genes (incomplete)         :: 61 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 11 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058963.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ZZW20"
                     /isolation_source="sewage"
                     /db_xref="taxon:573"
                     /plasmid="pZZW20-4.4K"
                     /geo_loc_name="China:Fujian"
                     /lat_lon="23.34 N 116.54 E"
                     /collection_date="2018"
                     /collected_by="Shuan-cheng Bai"
     gene            386..1300
                     /locus_tag="H0E90_RS28650"
                     /old_locus_tag="H0E90_28650"
     CDS             386..1300
                     /locus_tag="H0E90_RS28650"
                     /old_locus_tag="H0E90_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_017880353.1"
                     /translation="MPNTVNYPQIIPFISKGKMDAIKNTFGNTLTDRECYGIYIWSQKA
                     SAAIYPLLQQLEVTLRNSIDKEATNVMGLNWWDGVYTDKTKPKWDDFIFNINKGRDRYK
                     KNYKKNNPSVLSSLIVVPHDDAIAHTDFYTWQAVLSDTFHTQNRKHVNKSLWPRLTYKV
                     LKGLDRKKTEKAARLDFINELNEIRNYRNRLSHNDCIWIKMNSNNLQSAVETVREKINK
                     IEDLIKTINPQIHSTLVKWGVFYHARRICSQREVEHHMGKNIVNCTNPTMSSILATIYS
                     WTDDGKLTSVVNDGKNDLAIHKF"
                     /name="Abi family protein"
     gene            complement(2672..3094)
                     /locus_tag="H0E90_RS28655"
                     /old_locus_tag="H0E90_28655"
     CDS             complement(2672..3094)
                     /locus_tag="H0E90_RS28655"
                     /old_locus_tag="H0E90_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004216558.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVID
                     TSELIRVFGSLDGVIDAVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3094..3285)
                     /locus_tag="H0E90_RS28660"
                     /old_locus_tag="H0E90_28660"
     CDS             complement(3094..3285)
                     /locus_tag="H0E90_RS28660"
                     /old_locus_tag="H0E90_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3766..3915)
                     /locus_tag="H0E90_RS28665"
                     /old_locus_tag="H0E90_28665"
     CDS             complement(3766..3915)
                     /locus_tag="H0E90_RS28665"
                     /old_locus_tag="H0E90_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017880354.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     Typing          2..316
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.478"
                     /coverage="91.0"
                     /evalue="6.8e-82"
     Typing          4071..4436
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.275"
                     /coverage="99.0"
                     /evalue="7.55e-176"
ORIGIN
        1 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
       61 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggatag acgtgcaaat
      121 gcgccactgg caacggccac tggtaagcag gatgtgcgag agatgtatcg agagttcttg
      181 aagtggaggc cttagtgagg ctacactgga aggacagatt tggtgacatg tctcgcacaa
      241 gactgttacc acggttcaga aacttgccgg ttaatgaacc ttcgaaaaac caccttgccg
      301 ggtggttttt tcgtttcata ctctacaact atgctgagcc atatggcaac atcaattcat
      361 acctacatat gaagaagcga tgcccatgcc taacaccgtt aattatccgc aaataatccc
      421 gttcatatcg aaagggaaaa tggatgctat aaaaaacact ttcggtaata ccttaacaga
      481 cagggaatgc tacgggatat atatatggtc gcaaaaagcg tctgctgcaa tataccctct
      541 cctacagcag ttagaggtaa cgcttaggaa ctcaatagat aaagaagcaa caaatgttat
      601 gggtctgaac tggtgggatg gagtctatac agataaaacc aaacctaaat gggatgattt
      661 tattttcaac ataaacaaag gccgagatag atataagaag aattataaaa aaaacaaccc
      721 atctgtactc agctctttga ttgtcgttcc gcatgatgac gctatagctc atactgactt
      781 ttacacatgg caagcggtcc taagtgacac atttcacacg caaaatagaa aacacgtaaa
      841 taaatctctc tggccaaggc ttacttacaa ggtgctaaaa ggtctagaca gaaaaaaaac
      901 agagaaagca gcaaggcttg attttatcaa cgaactaaat gagataagaa attacagaaa
      961 cagattatcg cacaatgact gcatatggat aaaaatgaac tccaataact tacagtccgc
     1021 agtggaaaca gttagagaaa aaataaataa aattgaagac ttaataaaaa caattaaccc
     1081 tcaaatacat tcaaccctgg ttaaatgggg agttttctac catgcgagaa gaatttgctc
     1141 tcaaagagag gttgagcatc atatggggaa aaacatagta aactgcacca acccaactat
     1201 gagctctatt ttagcaacca tttactcatg gacggacgat ggaaagctaa ccagcgtcgt
     1261 gaatgacggt aaaaacgacc ttgcgataca taagttctag aaggagaaaa ggcccgacat
     1321 gctaagcatg tcgagccttt tccaagtctt ttgccaatat caccttacgg cggagcgtgg
     1381 cctttattcc attataaata atgcataaaa gcgaaagctc taggcttcat taaaaatgat
     1441 tctacatcac ttactcaaaa aagagcaaga ccttatgtca caagggatca cacgagcagc
     1501 cgtaaatgac ttaacttgat gaaaaaaaac gtttttaatc taactaaact tacctgcctg
     1561 gctttaataa agagcttaaa aatggtacaa tcaaccatcc aagcaattgt tttataaagt
     1621 caattagcgt tccatcagac aaaaaataat ggagagaaag gagaatgaaa acaatgaaaa
     1681 cgttctttaa ggttaaaaat cttttaaaat caatgagata cttaaagcgt ttcctgtcac
     1741 tgaatgcggc agtgaaacgc gagtgcgcgg tggtgtgtaa caccttcgct gtcgcattca
     1801 gtgacaggca ctagtttatc acaaaaaacg ataaaagtca atataaccaa cactttaaaa
     1861 ccaaacattt tagctactcg aaacggtaat tttaacgaag tccccccccc tcaatacaaa
     1921 cccgaatatt aagctattta ccataacatc ccttgcgtgc accacctgag aggccctcag
     1981 ggcgtttctg acgggaaacc ggcaaaacag acaacaaacc acccgaaaac ccgccagaaa
     2041 cgcgcaggct ctgtttttac ggcatgcatg agtatgcgcg caagtgcata gcgcggaaat
     2101 tcacgccatt cagccggggt aagtcctgtt tccggcgttc ttcccatccg aaaacggtga
     2161 acggggacgc aatttattgc gtggccgtga agcgttgccg gcgcagccgg aaaaccggtt
     2221 ttcagcacgt tcaggaatcg ggttcgtcga cggccggcaa tcacgttatg gcaagtcgcc
     2281 ggacgactca ccacacgatt caccggcgat tcgactcgtg acttgactct agagtcacca
     2341 cggtcaaaaa aacgccaaat cgacgattcg actctagagt caagtcgtca taagttactg
     2401 atagtgtgga gtgagtcgtc gatgactcta tgacgagtcg tcggcaagtc gtacacgaat
     2461 caccgacgac ttggattgac cttacagaag gtaatactgc gtaatttaat aacaaccaac
     2521 taactgatat taatgagtta tctgcaaaaa tttaattttg cgtggggtgt ggatattttt
     2581 agtgtgagga gcgttcagag atctgacgat ttttgggaag gttcacgaac gccccggcat
     2641 ggccggagag gaaaactcaa gcatggcggg atcaggattt tttccagaat ttccaccacg
     2701 gggaatgggg tggtgaagaa ggttcgtgct tgtgctccag aagctgcata gcctgacgaa
     2761 gggaatcaat atgctcatct cgagccttaa cagccccttt aagaccatcg ttttcagctt
     2821 caagtaaagc gattctatgt tgtaaatcat gtaaaccaac gctgttaata cctgtagatt
     2881 ctgcgtcact ttttacatca tattttacag catcgattac accatcaaga gaaccaaaca
     2941 ctctaataag ctctgatgta tcaataactt taataccctt cccgtgcttt acaacagagg
     3001 tgtaacttaa cttaccactg ttaatcatct tgtaaatagt agggcgacta accttaacaa
     3061 gccgtgctgc ttcactaata gacactctgg ccatcagtta ccatcctgta atgcatccag
     3121 tctcgaggac agtgtacgga aaagatgctc cgcatcatcg tggagtttct cacaaaggtc
     3181 ggcctcagca tcgagatcaa gctcgttaag cttctccagc aggagcagag actgattctg
     3241 gatgaattta gccatgttca gcgcagtttt ctgttgtttc gtcataaggg acctcagagc
     3301 atcagtgctc agtgtaaaac cggtaagggg gtcggatgta cgaatggttc agagaacgga
     3361 tctgaggtaa tcctgtagtt attttgtagt ttatctgtag tcatcgcact aaaaatcagg
     3421 attaagtcat cagcttttgt agtctttctg tagtttacct gtagtccgga aaaataatcc
     3481 gttcatgcga gcatgaacga ggtcgttaat gacgtttttt tgtcaccgac gccccttaac
     3541 ctccgcagat cacccggcca gaatgataat tgtttggccc ttaaatggtc atacacctaa
     3601 cgcagccgcg attttcatcg caactgcatg aattaaaagg taaaaattga aacctccgtt
     3661 tatttatgta atacgttgcg tattacaatc acctgtaacc acatgaaaat aaagaaataa
     3721 aatgtaatac cggcgtatta ccacgtacgc gggcaagcga aaaagctata acgcttcccg
     3781 gccgacaggt ggcccggaat ccacaggggc gtgcaggttc tcttcagtaa cctgcgccat
     3841 taaatcaaaa aggtcatatt cgccgggaag ctgcgtatca gtcgaatcca gttgttcatc
     3901 atattcagat ggcatggggt gatatccagt agctgagagg ttgttagcgg gtttcggggc
     3961 gcagccctga accagtcaaa tagcacgtgc ggagtgtata cgggctcaat atgctacggt
     4021 ggagtaacac gttatgaaag tgcgtcatgt gacagaggaa agtgcagcgg cggggcgtac
     4081 gggagaaaat ggaaacccgg ggatatgacg cctcctcgct cactcagtcg ctgccgctcc
     4141 tgccgttcgg ctgcggcggg cgttacctgc acacggataa gagcaaagac atacggcatg
     4201 acaagcgggg cagcaaaaga atgcgccgct gccgtttttc cataggctcc gcccccctga
     4261 caagcatcac agaatcggac gctcaaatca gtggtggcga aacccgacag gactataaag
     4321 atatcaggcg tttccccctg atggctccct cgtgcgctct cctgttcctg cctttcggtt
     4381 taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactc
//
