LOCUS       NZ_CP058946             8917 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain BA39649 plasmid pCol440II, complete
            sequence.
ACCESSION   NZ_CP058946
VERSION     NZ_CP058946.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14402321
            Assembly: GCF_011742285.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Klebsiella pneumoniae VBA39649 hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
REFERENCE   3  (bases 1 to 8917)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 43.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 08:28:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,895
            CDSs (total)                      :: 5,763
            Genes (coding)                    :: 5,561
            CDSs (with protein)               :: 5,561
            Genes (RNA)                       :: 132
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 202
            CDSs (without protein)            :: 202
            Pseudo Genes (ambiguous residues) :: 0 of 202
            Pseudo Genes (frameshifted)       :: 106 of 202
            Pseudo Genes (incomplete)         :: 92 of 202
            Pseudo Genes (internal stop)      :: 26 of 202
            Pseudo Genes (multiple problems)  :: 20 of 202
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058946.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BA39649"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCol440II"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2019"
                     /collected_by="Dept of Microbiology, CMC Vellore"
     gene            complement(475..687)
                     /locus_tag="HBJ39_RS29215"
                     /old_locus_tag="HBJ39_029200"
     CDS             complement(475..687)
                     /locus_tag="HBJ39_RS29215"
                     /old_locus_tag="HBJ39_029200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(691..1176)
                     /locus_tag="HBJ39_RS29220"
                     /old_locus_tag="HBJ39_029205"
     CDS             complement(691..1176)
                     /locus_tag="HBJ39_RS29220"
                     /old_locus_tag="HBJ39_029205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1214..1867)
                     /locus_tag="HBJ39_RS29740"
                     /old_locus_tag="HBJ39_029210"
                     /pseudo=""
     CDS             complement(<1214..1867)
                     /locus_tag="HBJ39_RS29740"
                     /old_locus_tag="HBJ39_029210"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1854..>1919)
                     /locus_tag="HBJ39_RS29840"
                     /pseudo=""
     CDS             complement(1854..>1919)
                     /locus_tag="HBJ39_RS29840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(1953..2558)
                     /locus_tag="HBJ39_RS29230"
                     /old_locus_tag="HBJ39_029215"
     CDS             complement(1953..2558)
                     /locus_tag="HBJ39_RS29230"
                     /old_locus_tag="HBJ39_029215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509965.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGCAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            2652..5547
                     /locus_tag="HBJ39_RS29235"
                     /old_locus_tag="HBJ39_029220"
                     /pseudo=""
     CDS             2652..5547
                     /locus_tag="HBJ39_RS29235"
                     /old_locus_tag="HBJ39_029220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            complement(5544..5840)
                     /locus_tag="HBJ39_RS29240"
                     /old_locus_tag="HBJ39_029225"
     CDS             complement(5544..5840)
                     /locus_tag="HBJ39_RS29240"
                     /old_locus_tag="HBJ39_029225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_266097711.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIEGSVPAEGEMTP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6477..6638
                     /locus_tag="HBJ39_RS29245"
                     /old_locus_tag="HBJ39_029230"
     CDS             6477..6638
                     /locus_tag="HBJ39_RS29245"
                     /old_locus_tag="HBJ39_029230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     gene            complement(6791..7411)
                     /locus_tag="HBJ39_RS29250"
                     /old_locus_tag="HBJ39_029235"
     CDS             complement(6791..7411)
                     /locus_tag="HBJ39_RS29250"
                     /old_locus_tag="HBJ39_029235"
                     /inference="COORDINATES: protein motif:HMM:NF025084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(7404..8759)
                     /locus_tag="HBJ39_RS29255"
                     /old_locus_tag="HBJ39_029240"
     CDS             complement(7404..8759)
                     /locus_tag="HBJ39_RS29255"
                     /old_locus_tag="HBJ39_029240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_014386473.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     Typing          complement(5955..6127)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          6074..6587
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(360..1864)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.215"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5572..5840)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.011"
                     /coverage="85.0"
                     /evalue="1.24e-47"
ORIGIN
        1 tccggacagg ttcgccacaa acggggcggc agtcaggtaa aaccggcaac aaaccacccg
       61 atacaccgcc ccgttcgtga cagataaatt ttacccggct gcatggtcat gcagccagat
      121 gcatacggtc tgatttcagg ccagaatccg gatgaacgcc ttaaaaacag gaaaatgctt
      181 ctccagccag tcccagatct ccagcgccat gtacaggcaa ttcagcaccg tctcaatcac
      241 gttaaacagt ctcataatcc ctccgttgta tacgctgtga tggaaacatc acgaccgtat
      301 ttagcgggaa atttatgagc ccttcttctt ggggtttgtc actggatgcg cgcagtcagc
      361 ccagcgtcgg accgtcatag ctccgctgcc gggaacgggt ttccgccagc tctttttcat
      421 gctgtttctg ctcgtatgcc cgttcggcgg ccaccgccat ttcatgccgc tgcactactg
      481 gctcaaacgc tgtaactgcc tgctcaagtt cgccacctgc tgactcaagc gcgtgacgcg
      541 ctcgttcagc gccgcgttct cccgttgcat aagaccagac atcttctgcc attccgcgaa
      601 ggcgctctcc cactcgtcca gcctttgcga gtagtcctga tgtagctgct ctaatgcggt
      661 cagcaactgt ttttccagtt ccgtcatggg ctatttcctc tccagtacca tccagttccc
      721 ttcctccccg taagctcccg ctttcgggtt cactttgacg caggacagtt tcttctcccc
      781 gcagagcgac agctgcacgc cgtggttctt ctccgacagc atggcctgcg tccgctcctg
      841 cgcccggata gtctggtaat tcccggtgat ctgctgcccc tgccaccaca gaatgctccc
      901 gctcgtggcg atcagcagta ccgagaccag ggtgatggtc agccacgtcc ggccgaccat
      961 acgcagcacg ctcagccggt tcgactgcat cgccgcggtc ataccctgct cgtgggcgct
     1021 gatggcgtcg ctgattctct tcgcgcttgc gctcagttcc gcttttacag actgctcgtg
     1081 ctcgctgaat gcggctttca gcatctcgcc ggtactctgc tgctgcgctt tcgattgctg
     1141 ctccaagtcc ttcgccagcg ctaaaagact gctcataaat ggctcccttc agtcggatgt
     1201 ttcgcccccc gtccgggtcg gcaatgctga tactgttttt tgtggtgcgg accacctcaa
     1261 aacctgccgc tgtgagcgcc cctgtgacgt cctcgcgcgt tttgagctcc ccggatgagg
     1321 ctaaggccag caacccacgt gtaatcgcct gagcggcttc cagcttcgtt tccggcagtg
     1381 cggacggcgt gaccagtaac cgtcggttct ccggggcgtt tggatcatgc agccccagcc
     1441 tgccgttcac cacggtctgc caggcatcga tgcgcggccg gtcggcgcga tcgtagtacg
     1501 gctgcaggcg tttcccggtc agcagttcag tgttggggat caggaaattc agctccagcc
     1561 gccccttgtc ggtgtgctcc acccacagaa tgctgtactg gtctttatcg agcccgggca
     1621 tcagaacccg ctcaaagctg gccatgatct gctcccgctg cccgggcggc agttcagcct
     1681 ccgcgaacga cagcacgccg gaggtgtact ttttcgcata cggcgaggca tcgataagct
     1741 cccggacttc ctccggtttt ccctgcagca cggtcgcccc ctcgcgctgc cggtcttttc
     1801 ccagcaggta atccaccgga ccggcaccac caccgcgccc ccgggggtga aatttaacga
     1861 tcatcatccc cccctttttc cagcaccgca tgccgcagcc gctccagtcc cgcgtcgatg
     1921 gggtcggttc cggctgaggg cgaaatgaca ccctaagcgt tagctctgtg tcgttgcacg
     1981 atgtcagcga cggtattctt gctgataccg agctcgcgtg cgatccagcg atagctgcgt
     2041 ccctcggccc tcatcgcaac caccttaggc aaaagtcggt ctgattttgg tcgcactccg
     2101 gcctgacgac caagcctctt accacgtgcc ttcgcaacag caaggcctga cttgacccgc
     2161 tcgctgatga gatcccgctc aaactccgca atgccggaaa gaaacgtcgc cagcattcgt
     2221 ccatacggcg acgaaagatc gaacgccatt ccattcatgg ctatcacgga aaccttccag
     2281 ttctccagtt cacgtagcgt attgagcaga tcgagcgtcg agcgccccca ccgggaaagc
     2341 tcagtgacca ggattgcatc aatttgtctg gactgggcaa gcgccaggac tttctttcgc
     2401 tcggcccggt cgagtttagt tcctgaacct gtttccttaa atattcccac cacgtcgtag
     2461 ccggcacagc cggcgaaggc tcgcagatca aattcctggc gttcacaaga ctgatccgct
     2521 gttgaaaccc ggcagtaaat ggcggcacga tgtcccaatt gaaccctccg gatttttgta
     2581 tcggaacgcc ctgatttata tgggctggct gttgtccaaa acagactata cttcaaaagg
     2641 gacgaatttg tatgtcacga cgccatattt tcaccgaacg gcagcgagca gcgctgttcg
     2701 atctgcccac ggacgaactg tcgctactga agttctacac gctgggcgat gatgacctgg
     2761 aaaacattag gcagcgccgc agaccggaaa acaggattgg ctttgccctg caactttgtg
     2821 ccttacgata tccgggccgt gcactggctc ctggtgagat gatcccgcgt gaagtccttt
     2881 ccttcgtcgg tgctcagctt ggagttccgg ctgatgcgct tctcacttat gccacacggc
     2941 gccaaacccg tcagcagcac atggacacgc tgcgcgaaat ttacggctac aagaccttca
     3001 cgggccgtgg tgcccgtgat ctgcgggagt ggactttcgg ccaggccgaa gatgccagat
     3061 caaacgagga tcttgctcat cgttttattg tgcggtgtcg ggaaacttcc accattctgc
     3121 ccgcagtatc gacaatcgag cgcttgtgcg cggatgctct ggtcgccgct gagcggcgga
     3181 ttgaaacgcg gattgcggaa aatttaacag cggatgttcg cgatcacctg gacaaacttc
     3241 tgagtgaaat gctcgccggc aatatcagtc gtttcatctg gcttcgcaac ttcgaggttg
     3301 gtaacaactc ggctgctgct aaccgtttgc tcgacaggct cgaatttctg cgtaccctga
     3361 atatcaatca tagtgctttg gccagcatac ctgcccatcg cattgcccgg ctgcgtcggc
     3421 agggtgaacg ctacttcacc gacggtttgc gtgacatcac ttcggaccgc cgctgggcga
     3481 tccttgccgt ctgtgttgtg gagtgggaag cggcgattgc tgatgccata gtcgaaaccc
     3541 atgacaggat cgtaggaaaa cccggcggga agcgaagcgc cagcatgacg aaacaatttc
     3601 cggctctaaa gccacactca cggatacgat ccgtaccttc accgcgctgg gagcttcgtt
     3661 gcttgaggcc cgcagtgacg gaaccccgct ggagatggct gtcgccagtt cggttgcatg
     3721 ggaccggctc gctcaactgg tagcgacagg gactcaactc agcaacacgc tagccgatga
     3781 gcctcttgca tatgtcgggc agggatacca tcgctttcgt cgttatgcgc cccgcatgtt
     3841 gcgctgtctg aagctcgaag ccgcgccggt cgccggacca ttggtagcag cagctttgtc
     3901 gatcggagag atgaaaggtg ttgcatcgcc agaaaggcgt ttcctgcggc ccagctccaa
     3961 atggaaccgt catttacgag ctcaggaaaa aggagatacc cgtctttggg aagtggcggt
     4021 actctttcac ctccgggatg cttttcgttc cggagatgtc tggctcgctc attcgcgccg
     4081 ctatggtgac ctcaagcagg tactggtgcc gatgatcgcg gcgcaggaaa atgcaaaact
     4141 ggccgtgcct tccaacccac aggattggct ggcagacaga aaggcgcgac tcacgatcgc
     4201 tcttaagcgg ctggcccggg ctgcccgtaa cggcactatt ccgcacggta gcatagaaga
     4261 tggaacgttg cggatcgaca ggttgacagc agacgtgccg gatggtgccg aggcactcat
     4321 actggatctg tatcgccgaa tgccgtccgt tcggattacc gacatgctgc ttgaagttga
     4381 tgcagccctt ggtttcacag atgcgtttac ccatctgaga accggggctc catgtcgcga
     4441 ccggatcggt ctgctcaacg tcctgctcgc tgaagggctc aatctgggcc tgcgtaagat
     4501 ggcggaagct acaaacacgc atgattactg gcagctctca cgccttgccc gctggcatgt
     4561 tgaaagcgaa gccatgaacc aggcattggc aattgtggtg gccgcgcagg gtaaactgcc
     4621 gatgtcacgc gtctggggga tgggcacgtc agcatcgagc gatggtcagt ttttcccgac
     4681 agcgcggcat ggcgaagcca tgaacatggt caatgccaaa tatggttctg ttcccggcct
     4741 caaagcgtat actcacgtaa gcgaccagtt cgcgccattc gcttgtcagt cgatcccggc
     4801 gaccgtgagc gaggcaccgt atattctcga tggactactg atgaacgagg tcggtcgcca
     4861 tgttcgcgaa cagtatgccg atacagcagg attcaccgac catttgttcg gagccagtag
     4921 cctgctcggc tacaatctcg ttctgcgaat cagggatctg ccatcgaagc ggttgtacgt
     4981 atttaatccc gatacgaccc ccagggagtt acgcaagttg gtaggtggaa aagcccggga
     5041 ggatcttatc gttgcgaact ggcctgatat tttccgttgt gccgcgacga tgaccgctgg
     5101 caaaatcagg cccagccaac tcctgcgcaa gctcgcttct tacccacgac aaaacaacct
     5161 tgcagttgcg cttcgtgaag ttggtcgtat tgaacggacc cttttcatta ttgagtggat
     5221 ccggatacgg acatgcagcg gcgtgctcag atcggtctta acaagggaga ggcccaccat
     5281 gcgctcaaaa atgcgctccg tatcgggagg cagggggaaa ttcgcgatcg cacgacagag
     5341 gggcagcact accgaatcgc tgggctcaat ttattgactg cggtgatcat ttactggaat
     5401 accgtccatc ttggtcatgc cgtcacggag cggcggaacg aagggttgga tgttccccct
     5461 gaatttcttc cccacatatc cccattgggc tgggcgcaca ttctactgac tggcgaatat
     5521 ctttggccca aggaaccgaa agcttagggt gtcatttcgc cctcagccgg aaccgacccc
     5581 tcgattgcca tcagtgcggc cacaatctgc acccggtcat gaccggcacc accggcattc
     5641 actttccggg cgatctggtt caggttgttc cccataccgg caagctggcg cagcagcgcc
     5701 ggcgatatcg acggcagtct gccggcacgc gccggcttct catcgaggca ggtctgacgc
     5761 atccacgccg ccagctgctt gccgtcgcac cgctcgagca gccgccggtg ttcgtcttcc
     5821 gtcacccaca tcgtgagcat cttgttgcgt ttgtctgcca gagtagcctc ctgataacac
     5881 cgggacgggc gggaaatgtt tcccggtcgg cacggtgttg gacaagccgc aggcgcgtca
     5941 gtcgctttta gcgggtttcg gggcgcagcc ctgaaccagt cacgtagcgc tagcggagtg
     6001 tatactggct tattatgttg gcatgagtgc ggtaatcagg aaagtgcttc atgtgacaga
     6061 ggaaaatgca gctgcgggcg taaggcagaa aatggaagtt cagaggatat gacgcctcct
     6121 cgctcactca gtcgctgccg ctcctgccgt tcggctgcgg cgggcgttac ctgcacacgg
     6181 agaagagcga aggcatatgg catgacaaac ggggcagaaa aaggatgcgc cgctgccgtt
     6241 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
     6301 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggtggct ccctcgtgcg
     6361 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc
     6421 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
     6481 acgaaccccc cgttcagtcc gaccgctgcg ccttctccgg taactatcat cttgagtcca
     6541 acccggaaag acacggttca tcgccactgg cagcagccat tggtaacgga ttagaggaag
     6601 tgagtcttga agttctgcgc cagttaaggc taaactgaaa ggacaatttt tggtgactgc
     6661 gctccctcta agccagttac ccggtacata agttggtagc tcatgtacct tccaaaaacc
     6721 accgttggta gcggtggttt ttatttttca tagattacag cctgtaataa gccatggtca
     6781 atggattact tcaaaagtcg tcaatcgcct taagtagctc atggaatgat gtataacatg
     6841 gaaatagata aggttcgtgc gtgtttttat aattagtttt agcgtaaata tctagctttc
     6901 ttgcgttatc tttcgcagta ccaatcctct ctcttatagc aaggtaagtt agtttactac
     6961 ctttatcata gttagttatt aattttttta gcggggagct agacatcaaa ttatcatacg
     7021 acgaaaaatc tttggttgtg aagccgaaat gcaacaataa ccataattca aaacaaacac
     7081 aggatattgc tatatttatc ccatgcctat tagctttatt ccatgccttt tgatgacatg
     7141 aaatagacga aggcgtcaga tgctcttgat cataaacaac ccaaaagacg tcaccttctg
     7201 cggaagatga tgaattcttt ttctttatcg cctcatccac caactgctca ggtgtatttt
     7261 tccttgtttt aggtatttta aatacactta aagttctttt agatctgtct gaaatataag
     7321 cctcaaggta taatggttcg gttttctcac cctcacagta tatatacatt acttgtggga
     7381 ctttcttatt atttccatgt tttttactca tcagaaagat ccccgtcaaa ctcttcaaaa
     7441 aaaccatcag acaattcttt gacgtcaata tccggcatta ccaaggatat atcaccagat
     7501 ataaatgagg ctacctttaa gtagtttatg tcgggaaccc caccaaacct accctcatca
     7561 taccaagctg cgtaaggtgt tgttgttttt atctttttct tatcaaaatc atctaagcta
     7621 taaagagacg acgcaccttt atttttttca caaaaccata tctgatctct tctcattttc
     7681 tctggagcca ttaactgcat attatgggtt gagaatacaa gttgtgagcc ttttttattg
     7741 acatcaggat cattaaataa cttaacaatc aagtcagcaa tatgcgggtg caagctatta
     7801 tctaactcat caacaattac aaccatacta tcattaaacg cagatctaat tagaggtatg
     7861 atttcaaata atttttgggt gccttcagac tctctcccca tagggaaaga aacactcccc
     7921 ccttcatcag taggatgaga gaacatataa cgatatttat aatcttcttc aattatctcc
     7981 tttatttctt gagggatttc ctcatccatt tttattggta tttccgcctc aacctcccgt
     8041 acagatatac cggaaacacc agtgtcaacc atgcataaaa ccttggccgt ttcatttacg
     8101 acgtcctccc tctccccaaa agaagatatt cttatctttt catttaaacc tatatgtcta
     8161 attccctttc taaaaaagtt ataagcttct ttaattatgt ctggtgatgc ggcattatta
     8221 cctgctttcg ctaagtatga attattaggg aagaatggta tttttttaat acctccttta
     8281 taatgcccac caaacttgat tgtttcccag gtgtcgctct catctctagt aaataaattt
     8341 gctttaaccc tagatggata atagtctaat gattcaaata aaatccgatt tttaataaaa
     8401 gcaacttcat aactgaaccg attcccctca acagaataaa attcagcctc aaactttaca
     8461 ggagcctcat tcgtagccga agaaagagca tatggctcat agcagggaat ccgtttacct
     8521 tctttcaaat caccgctttc tgttgcgata aattttatag cttcaaaagc catgagaacg
     8581 ttagatttac cagacgcatt agcaccataa atcccagcac tcatgagcgt tccaacattc
     8641 attccatcat ttttatagat atgagttgtt aaatggttac tagaaccgga tgcgctcaag
     8701 tttaatgtcg atttttcctt aaccgaccta aaattttcaa tagtcaaata aacaaacatg
     8761 catacctcct aacggggatt tttcgccaaa aaagcgaaaa atcaatcata ggaaagatat
     8821 cctatatgtt ttaattttgg aacaacatct tccttaaaaa acgttgtgct atttataatt
     8881 acacgctatt taccataaca cctgttgccc gcaccgc
//
