LOCUS       NZ_CP058900             4072 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Escherichia coli strain MS10858 plasmid pMS10858C, complete
            sequence.
ACCESSION   NZ_CP058900
VERSION     NZ_CP058900.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10141460
            Assembly: GCF_030988725.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   White,R.T.
  TITLE     Escherichia coli ST1193 strain MS10858 plasmid pMS10858C
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   White,R.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2020) School of Chemistry and Molecular
            Biosciences, The University of Queensland, Building 76, Cooper Road,
            St Lucia, QLD 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Flye v. 2.5
            Assembly Name                     :: MS10858_QU054_ST1193
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 276.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/31/2023 16:46:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,941
            CDSs (total)                      :: 4,822
            Genes (coding)                    :: 4,590
            CDSs (with protein)               :: 4,590
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 100 of 232
            Pseudo Genes (incomplete)         :: 149 of 232
            Pseudo Genes (internal stop)      :: 38 of 232
            Pseudo Genes (multiple problems)  :: 51 of 232
            Pseudo Genes (short protein)      :: 4 of 232
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058900.1.
            Bacteria and source DNA available from Escherichia coli.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS10858"
                     /serotype="O75:H5"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMS10858C"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2007"
     gene            join(2698..4072,1..773)
                     /gene="mobQ"
                     /locus_tag="MS10858_RS24695"
     CDS             join(2698..4072,1..773)
                     /gene="mobQ"
                     /locus_tag="MS10858_RS24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(1031..1732)
                     /locus_tag="MS10858_RS24700"
                     /old_locus_tag="MS10858_C0002"
     CDS             complement(1031..1732)
                     /locus_tag="MS10858_RS24700"
                     /old_locus_tag="MS10858_C0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2265..2519)
                     /gene="mobC"
                     /locus_tag="MS10858_RS24705"
                     /old_locus_tag="MS10858_C0003"
     CDS             complement(2265..2519)
                     /gene="mobC"
                     /locus_tag="MS10858_RS24705"
                     /old_locus_tag="MS10858_C0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2521..2697)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2698..3056
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1032..1732)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..770
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.922"
                     /coverage="99.0"
                     /evalue="1.12e-164"
     Typing          2612..4071
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.538"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttcacaaccg gaacggagaa tatacgctct gggggaacga gctggagaaa taccgcacca
       61 gaatctttga gagtgttgat ttaatgcgtg acaggagtgg ttatatctgt gaccgaagcg
      121 aaatcggcca acgtccgcca ctgcaacgtg tttattcctc tgcaaccttt gagcaactgc
      181 tgacgcaggt ttgccagaca tggccgcaac atacccgcaa tctccgccag ccgaagacat
      241 ggccggagtc tttctgtctt ggagaagacc gccagccagc catgccatca ccagcggcaa
      301 ggaaggtgga ttttatgcag ggacgtctgc caccgacgct gatgccggtc atgagttctg
      361 tcgaccggga aacccgccag cttcagcttt tgctggtcat gggcgtggat gactctctgg
      421 gcggtgttat ccggcttaat ggtacgctgt atcccgcgtt tgccgttccc tcagccgata
      481 acaggcatct ggtcatcagt gcgctgaccg ataaggggtt acggtatgca gggtatggtg
      541 ttgccgtaaa tcacgatgca gacagccaca tatcacctgc accagaactg atgcagtttc
      601 atctgaaaac ccgtgaggca ccgctttttg ctgccgtcaa cacaccagaa aaacagccgg
      661 atcatctgtt ccgctcgctg ggttttaatc gtacctggga ggaatggcag cgcgaggaag
      721 atgcacgaac acacaccacc gagaggagac atgatcgcgg ttggtcacag tgaatgtgaa
      781 caatattttg ttttgtttaa catcgtgtta tgttcgtcga tgttcttcga tgttcatcga
      841 cagggatgaa tgcaagaagc acactcacat aacgcaagca tagttttgtt tttccgcccc
      901 caaaaattgg ggggtctttt ttcgctgcat cagagcgttt ttgacgcaat ttttcacttc
      961 aatagtacct tcgcactact aacacaaaaa aaacgcagca gggagcgttt taggtgcctt
     1021 aaaatcgatt ttagatcgtc atcttgatct gtttgtcttc cttgaagcga aaatgcaggg
     1081 ctacaaccgt gcgccccttt ttgatcgtct caacagttac aaccagatcg cttttttcgt
     1141 tcagttcggc gatggctggc ttgattatca tcttattcaa gtctttgaat tgttgatatt
     1201 tatcttcaac ggcaagcatg gttcggaaat catcgatagc tatcgttctg tcaccggtgc
     1261 tgcggaactg ctggagcagc tcgtaaatgc ggatgctgta ggtgctggaa aggctggaga
     1321 cgttcttcac gactacccgc gtaaactgcc ccttgagctg tgtcagatac ggcatgatgg
     1381 catcggagaa ggtgatatct actttcgcct cacctttgaa atactgcgcc cggtactgca
     1441 cccagcggaa ctcttctcgt ttttcctcgt tcttgatgat tactgacctg tcccagaggc
     1501 gatctatcgc ttccttgagc ctagcttcag cgtgctgccg tcccatgtca ggaaaggcat
     1561 catgaaactc tgcagccgtg atggtcatgg ttttttgttc atccggcgaa ggggaatctt
     1621 ctccggattt caggcggctg atacagacca gcaggaagcg ctgttcctgc aaagtgagct
     1681 tgtagcttgc ctcgataagg gcgtttgatt tgtaagctat cagatctgac atttcgcttg
     1741 ctcgttgcta ttggtggcgt ttttttactg tacgccactt atcatatggg tggaagtttt
     1801 ttttttgcaa cccccttttt ctgtggatta cacccccaaa agctccacct ttcaccccca
     1861 aaagctccac ctttcacccc caaaagctcc acctttcacc cccaaaagct ccacttatct
     1921 tgccagaaca tctttaaaat catggtgtta caagacctaa aaaggtttta aaaaaaagat
     1981 ctaaaaataa gatcttaaaa acttaacggc gagcctgtgg ataacttggg gtgctgaaaa
     2041 caaaaaacca ataaaaacaa tgcgttgaaa taaagcagaa ttgcaagttt cgaatacacc
     2101 ccctccaacc ctgtggaaac ccagtattca tgcgggttca gcgcatatca cctgccgaaa
     2161 aaccgtgcgt atgtgctcaa cctggcgact gattcacttc cgcttcctcg cccactcgct
     2221 cgctaacgct cggtcgctgg ggctgcggcg agcggacgat acaatcactg ttctggcagc
     2281 gcctccagct cgttaatcag cggcagtatg cgcgtcacat cagcctgtcg tgtcacctct
     2341 gccttcagga actggagtaa ccaggcacgc aactgcacct cctccggctt tctgaccttt
     2401 gccagcaatg cagcacccac aacaaccttc tgcgctgtgt ccaggctccg gctcttcgcc
     2461 ttcaggcgct gtaatctcgc ctcagcatca gcaatcttct gttcgagcgt tctgctcatg
     2521 atgtaactcc gtgcgcgatg aaaaatcgca ttttagcgcg tcactggtag tttaaaaact
     2581 gagctggcat aatgcacggc aaatcacgaa gtgcgcactt atacaatctc cactccgttt
     2641 cgattgtgtg cgccctgcgg ggctgaaaga aaactacaaa aaggcattac ggcagaaatg
     2701 gcgatttatc atctcagcat gaaaattatc tccagaagca gcggctacag cgccgtagct
     2761 tccgctgcct atcgttccgg ttcactgatg cttgatgagc gtaccggact gactcacgac
     2821 tacacccgca aaagcggtgt ggctgaggct gtgattctga cccccgccac cgcccctgcc
     2881 tggtgtacga accgtgccga actctggaat gccgttgaga aggccgaacg ccggaagaac
     2941 tcacaacttg cgcgggaaat tgaactcgcc atcccccacg aactgccgca ggatgccgca
     3001 cgggaaaccg ttctcgcctt tgtcagagaa aactttgtca gtcagggcat gattgctgat
     3061 gtggcgttcc atcacatgga taaaacaaat ccccatgcgc acatcatgct gaccacgagg
     3121 gctatcggac ctgctgggtt cagcggaaag gtcagggact ggaacgaccg gacacatgcc
     3181 gagacgtggc gggcatcatg ggctgaccat gcaaacaggg cactggcgaa cgccggttat
     3241 caggaagaga tagaccatcg ttcatacgag cgacaggggc ttgagaaaac gcctggtatt
     3301 cacctcggaa agtctgcctg tgcgatggaa acacgcggta tcgaaacaga acgcggcgaa
     3361 cagaatcgtc tgattaacag gcttaacctg gaaatacaga tttcccgcac cgaactcagg
     3421 aacggagggc tgacagagcc tgcccgccgt gtggctgacc ttctgaatat cgggcttcct
     3481 gacaacgcaa cttcgtacac actacgtgac attatcgaag cgttgccgaa ggacagtaac
     3541 tcggcatgga aactcaccag taatgccatg tcgatgatgg cagatatgca ggcgacgcgc
     3601 agacgctggg aagagctgaa caccgaacga aaggaagcga tgagccatgc tgccagcctg
     3661 aaaaaatcat cgccgttttc gtccggtttt tcccgtattc ccctgatgca atgggcggca
     3721 ccggaatacc gtagtgagca gggaaaaatc cagtctctga gccagcaaat ggaaaaatta
     3781 cgacagcatt accggaaggt ggagaaacag gacattcccg cgagtcagat ggcatttgaa
     3841 aaacagtggc agcagtgggg ggaatccggc cttgcagctc tcagagccca gttgcttaaa
     3901 cgtgaagagg aattgcgccg gaaggaacag gaagatactg aacgtagtcg cgcggagcag
     3961 ctcaggaaaa atgacaatgc cgttatggat tcaggggtgc tgagagcagt cgttacgggg
     4021 tatggcgagg ctcccatgcc tggtaacggg gaaatgacct gttaccttct gc
//
