LOCUS       NZ_CP058899             2113 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Escherichia coli strain MS10858 plasmid pMS10858B, complete
            sequence.
ACCESSION   NZ_CP058899
VERSION     NZ_CP058899.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10141460
            Assembly: GCF_030988725.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   White,R.T.
  TITLE     Escherichia coli ST1193 strain MS10858 plasmid pMS10858B
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   White,R.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2020) School of Chemistry and Molecular
            Biosciences, The University of Queensland, Building 76, Cooper Road,
            St Lucia, QLD 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Flye v. 2.5
            Assembly Name                     :: MS10858_QU054_ST1193
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 276.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/31/2023 16:46:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,941
            CDSs (total)                      :: 4,822
            Genes (coding)                    :: 4,590
            CDSs (with protein)               :: 4,590
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 100 of 232
            Pseudo Genes (incomplete)         :: 149 of 232
            Pseudo Genes (internal stop)      :: 38 of 232
            Pseudo Genes (multiple problems)  :: 51 of 232
            Pseudo Genes (short protein)      :: 4 of 232
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058899.1.
            Bacteria and source DNA available from Escherichia coli.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS10858"
                     /serotype="O75:H5"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMS10858B"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2007"
     gene            complement(176..442)
                     /locus_tag="MS10858_RS24685"
                     /old_locus_tag="MS10858_B0001"
     CDS             complement(176..442)
                     /locus_tag="MS10858_RS24685"
                     /old_locus_tag="MS10858_B0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(446..1411)
                     /locus_tag="MS10858_RS24690"
                     /old_locus_tag="MS10858_B0002"
     CDS             complement(446..1411)
                     /locus_tag="MS10858_RS24690"
                     /old_locus_tag="MS10858_B0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(787..1018)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 aacatttgac atcggattgt ctatcctgca ttggtgtcga gttggcaagg ctgaggataa
       61 tgctgaggat aatgctgagg ataatgctga ggataatgca ggatagacag agcactagag
      121 attgcatgca atctcgtacc tatcacctca gcctgccgct ggctcttttc cagaactaag
      181 aatgcgttct taaaaatcca aacttgatac cgtttaaatc tattttcaca aaaagagttt
      241 ttaccctctc gtttgttttc tgaatatata aaattaattc atcctcaaaa ccttcgtgcg
      301 gatgaaaacc aaatgtgcca aaacttaatc catcaataag caaaataagg tttttaaacc
      361 tgtcaggaat caccgcttca cttcttaaaa aaaataaacc aatagtctta tttccattca
      421 ggtattcaat attaatattc atacgttaat ccgatttatc cttctcaggg gcgcgtttgt
      481 aacgccgttt acctgaatcc cagacaaacg ccgtccgctt cccgtcatca gtgccatccc
      541 ctacatcgtc ggcaatgaca agatcctcat tggtttctcg atccaactgg aggacgtttt
      601 ttagcgcccc accggtcgag ataaaacggc gcttgtgaag ctgccgcgtc agctcaagaa
      661 accactcagg atcgtttgcc atatcttccg gtttaacgga gtatttcagc gtttcagcaa
      721 ccgcgctttg cagttgctcg gcaacgttgg ccacaacctc acctgtctta gtttttactg
      781 cccgaatatc gatattcggc tcatagttca cccgcaagca atcgcgccag agttctaccc
      841 aacgttcgtg cttaacgtag ttcttccctt taaaccaaga aggttgcacc atcagcagac
      901 agtgaaaatg cggatgtgcg ctgccatcct tacctcgcgt cacctccgta gccctgatcc
      961 acccctgaac aggtgatagc tcctttcgct tttccattcg cttaaacgcc gcattcattg
     1021 ctgtaaggac tgttccaagt tcacctatct cgcagttcct gacagttaac gtcagaaaca
     1081 accatcggga agacgggtaa tccacaacga ttttcggtag agcctgataa aaacgggctt
     1141 gccacatgag ggttcttctc cactggcaga cagggcaatg acggacacgg caaaattgcg
     1201 cactacgaag ccgtaggcgc gtttctccgg tttccatgat ggtagaccaa ccaaaacgta
     1261 acaaaccgct acaggaggcc atacggagtg catagcgctc gaattcagca gcagtctggt
     1321 agataccccc gacagaatcg gtaagcgtgc gctgggtatc ccaaactgca tcacgggggc
     1381 tgtagtccga aaggaattta tcttctgaca ttaagcctgc tccgatctgg gcaggctgtg
     1441 atttgatgtg gtatattcat cacgtacctg tgcattgcga gccagtacat gtcaccagag
     1501 tttgccgctg aggtgacaca ttcgaaatgt tggggagtgt tgacgcactc cccaacgccg
     1561 attaaaaacg cctcaattct gctccatttt ctttgacctg tcaaaaccca ctcaaagcct
     1621 cgtcattact gggctaaacc actatttcat atccgccatt tatcccttaa gtataaatac
     1681 aaggatcacg cgcgagaatt gcgcaaaaaa taaccacaat tttaaccccc caaaacaagc
     1741 acccccgaac ggcttacccc aaccttttcc acacacccga tcaccaaaac agggaataaa
     1801 aacaaaaccc tgtcttacag gtggatggcc tcacttcgtg aaaccatcca ccccgcataa
     1861 gttttttaat cggtggtgtc gtcaatcact aaatcggaac cccaaagggg acccccacga
     1921 tttagcattt gacgagatta actctcggag atggcatcat gcttttaacg gactttaaaa
     1981 aacggtgaac ggatgcggat tttaatccgt gtctgtgagg cgttgagcaa cgagcaaagc
     2041 gaggcggtag aaagagagcc agcggcaggc tcaggacgat aatccgggga aaatgtacca
     2101 ctcgcagagt gtt
//
