LOCUS       NZ_CP058885             2107 bp    DNA     circular CON 30-APR-2024
DEFINITION  Escherichia coli strain STLEFF_29 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP058885
VERSION     NZ_CP058885.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925558
            Assembly: GCF_022494075.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. Unicycler 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494075.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 04:36:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,127
            CDSs (total)                      :: 5,009
            Genes (coding)                    :: 4,863
            CDSs (with protein)               :: 4,863
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 49 of 146
            Pseudo Genes (incomplete)         :: 101 of 146
            Pseudo Genes (internal stop)      :: 22 of 146
            Pseudo Genes (multiple problems)  :: 25 of 146
            Pseudo Genes (short protein)      :: 4 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058885.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STLEFF_29"
                     /isolation_source="Stanley WWTPs_29 WWTPs effluent"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.2 N 114.2 E"
                     /collection_date="2018-12"
     gene            31..222
                     /locus_tag="HZT24_RS25520"
                     /old_locus_tag="HZT24_25405"
     CDS             31..222
                     /locus_tag="HZT24_RS25520"
                     /old_locus_tag="HZT24_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(990..1106)
                     /locus_tag="HZT24_RS25525"
                     /old_locus_tag="HZT24_25410"
     CDS             complement(990..1106)
                     /locus_tag="HZT24_RS25525"
                     /old_locus_tag="HZT24_25410"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_142293809.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKAISRVFRSKR"
                     /name="small membrane protein"
     Typing          complement(1610..1959)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.455"
                     /coverage="85.0"
                     /evalue="4.68e-157"
ORIGIN
        1 tacagtaagc actttcgcgc cggggccttc atgaacaaac aacaaaagac tgccgttaac
       61 atggctaaat tcatccagga tcagtccctg cttcttctgg acagacttaa tgagctggat
      121 ctggatcatg aggctgattt ttgcgagaaa ctccatgagg atgcggaacg cctttaccga
      181 agcctgtcag cgcgcctgaa tgagcagcag gacggtgcgt aatgtctccg ttctgagtat
      241 taccggagta ataccggcat cgccttaatt ttcatagtgt tagcgcttaa cattattacc
      301 ccggtaatac ccagaatccc tgtatttttt cttttttatt tatttttcat gaagttgcga
      361 tgaaaatcgc agctgcgtta ggtgtatgac cattttgagg ttgaaaaata atcaaccatg
      421 gcgggtgatt tacgcaggtt aaggggagat cgtgacaaaa aaaagcgttc ctcactgtat
      481 cgttcatgcc tgcatgaacg ggttattctt cctgactaca gaattacatc gctccttcag
      541 gtcatacatc acttttacaa cacttttaca tcactccttc gctctgtaca tcacttttac
      601 aacacttttg catcactttt acatcattcc ttcagctcat acgtcacttc catcctctgt
      661 gccatttgta catccccccc cttcctgaac gtgctgaaaa cggtttttcc ggctgcgccg
      721 gcaacgcctc acggccacgc aataaattgc gtccccgttc accgttttcg gatgagaaga
      781 acgtcgaaaa caggacttcc actggctgca cggtgtgaaa ttctgcgcta tgcacttgcg
      841 cgcatactca tgcatgccgt aaaaacagag cctgcgcgtt tctggcgggt tttcgggtgg
      901 tttgttgcct gttttaccgg tttcccgtca gaaacgccct gaggccgttt tcgcggtgcg
      961 cgtaagaagg gattatggta aatagggcgc tatcgcttac tcctaaatac acgactgatt
     1021 gctttttttt ctctgaagta tgagattcct gagtagacag ctacaacaag tagagcaatg
     1081 gcgatagcaa gtgaaatcca gtccataaat gcctgattaa tttaataaaa acagcctgtt
     1141 atgatttcat aaagataata tcatcttttt tttagcatga tactgtcgtt acttattgtg
     1201 gtgagatgaa acatagccac tgtttttgta tgtgtttgtt aaggccgtta agattattct
     1261 taatcactga ttttgtacat atcatcaaaa taaaaataaa aaaaactaat gcccccgtgg
     1321 tgggtatata acaaatcacg gggtgcaggt cagaactacc ccaaaacttt gaatgatcgg
     1381 gtggcagact aaatgaaatc ttgagattca ttcagcctcg tcgtaatctc tttctctgta
     1441 aacgaaaaaa ccgccctgca aggcggtttt tcgaaggttc agtaagttgg ggaacttctg
     1501 aaccgtggta acagtcttat gcgagacgtg tcacccaagc tgtccttcta gtgtagccgt
     1561 agtaagggca ccacttcaag aactctcaca atatctctcg cacatcctgt taccagtggc
     1621 tgctgccagt ggcgcatttg catgtctttc cgggttggac tcaagacgat agttaccgga
     1681 taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     1741 tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggaa
     1801 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcta ccagggggaa
     1861 acgcctggga tctttatagt cctgtcgggt ttcgccgcca ctgatttgag cgtcagattt
     1921 tgtgatgctt gtcagggggg cggagcctat ggaaaaacgc cccggcgggg cgtggccttt
     1981 tcgcccttgt ccttagcttt cgttatgctt accgttacgt tatctgctta tcctccctcc
     2041 gctcgccgca gactcatgac cgaacggagt gagcgaatga gcgaggaagc ggaaaaccat
     2101 cagaacg
//
