LOCUS       NZ_CP058878             6879 bp    DNA     circular CON 30-APR-2024
DEFINITION  Escherichia coli strain STLEFF_47 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP058878
VERSION     NZ_CP058878.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925566
            Assembly: GCF_022494235.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. Unicycler 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494235.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 04:36:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,232
            CDSs (total)                      :: 5,111
            Genes (coding)                    :: 4,880
            CDSs (with protein)               :: 4,880
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 90 of 231
            Pseudo Genes (incomplete)         :: 155 of 231
            Pseudo Genes (internal stop)      :: 50 of 231
            Pseudo Genes (multiple problems)  :: 60 of 231
            Pseudo Genes (short protein)      :: 5 of 231
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058878.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STLEFF_47"
                     /isolation_source="Stanley WWTPs_47 WWTPs effluent"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.2 N 114.2 E"
                     /collection_date="2018-12"
     gene            445..681
                     /locus_tag="HZT32_RS26285"
                     /old_locus_tag="HZT32_25750"
     CDS             445..681
                     /locus_tag="HZT32_RS26285"
                     /old_locus_tag="HZT32_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033806376.1"
                     /translation="MEKAPVPRPFPGSSFFAHMFFPGLSPDSVDNGVTAFEKGESARRS
                     RTTERSESVSEEAEEHRKLHFLLTHLCGISHSA"
                     /name="hypothetical protein"
     gene            complement(870..1061)
                     /locus_tag="HZT32_RS25985"
                     /old_locus_tag="HZT32_25755"
     CDS             complement(870..1061)
                     /locus_tag="HZT32_RS25985"
                     /old_locus_tag="HZT32_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_148119661.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELNADDQADICEALHDHA
                     DELYRSCLARFGDNGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1129..1452
                     /locus_tag="HZT32_RS25990"
                     /old_locus_tag="HZT32_25760"
     CDS             1129..1452
                     /locus_tag="HZT32_RS25990"
                     /old_locus_tag="HZT32_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1661..>2095
                     /locus_tag="HZT32_RS25995"
                     /old_locus_tag="HZT32_25765"
                     /pseudo=""
     CDS             1661..>2095
                     /locus_tag="HZT32_RS25995"
                     /old_locus_tag="HZT32_25765"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2130..2690
                     /locus_tag="HZT32_RS26000"
                     /old_locus_tag="HZT32_25770"
     CDS             2130..2690
                     /locus_tag="HZT32_RS26000"
                     /old_locus_tag="HZT32_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_074167501.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2697..2930
                     /locus_tag="HZT32_RS26005"
                     /old_locus_tag="HZT32_25775"
     CDS             2697..2930
                     /locus_tag="HZT32_RS26005"
                     /old_locus_tag="HZT32_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3414..3836)
                     /locus_tag="HZT32_RS26010"
                     /old_locus_tag="HZT32_25780"
     CDS             complement(3414..3836)
                     /locus_tag="HZT32_RS26010"
                     /old_locus_tag="HZT32_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_021539380.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3958..4236
                     /locus_tag="HZT32_RS26015"
                     /old_locus_tag="HZT32_25785"
     CDS             3958..4236
                     /locus_tag="HZT32_RS26015"
                     /old_locus_tag="HZT32_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(4228..4518)
                     /locus_tag="HZT32_RS26020"
                     /old_locus_tag="HZT32_25790"
     CDS             complement(4228..4518)
                     /locus_tag="HZT32_RS26020"
                     /old_locus_tag="HZT32_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            4763..6235
                     /gene="cta"
                     /locus_tag="HZT32_RS26025"
                     /old_locus_tag="HZT32_25795"
     CDS             4763..6235
                     /gene="cta"
                     /locus_tag="HZT32_RS26025"
                     /old_locus_tag="HZT32_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin 10"
                     /protein_id="WP_033801039.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVGG
                     GGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQRLK
                     DIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALREAE
                     RQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELAKAES
                     DVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSVTNALN
                     SAVSIRDAKKTEVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEKYSRIAQ
                     DLAEKAKGSKFNSVDEALAAFEKYKNVLDKKFSKVDRDDIFNALESITYDEWAKHLEKI
                     SRALKVTGYLSFGHDVWDGTLKGLKTGDWKPLFVTLEKSAVDFGVAKIVALMFSFIVGA
                     PLGFWGIAIITGIVSSYIGDDELNKLNELLGI"
                     /name="cta"
     gene            complement(6232..6522)
                     /gene="cti"
                     /locus_tag="HZT32_RS26030"
                     /old_locus_tag="HZT32_25800"
     CDS             complement(6232..6522)
                     /gene="cti"
                     /locus_tag="HZT32_RS26030"
                     /old_locus_tag="HZT32_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 immunity protein"
                     /protein_id="WP_072023376.1"
                     /translation="MTVKYYLHNLLESLIPWLFYLLLNYKTPPFSLFIFIASIHVLLYP
                     YSKLTIFSFIQNTTKMKKEPWYSYNLFYFLYLAMAIPVGLPSFIYYSLKRN"
                     /name="cti"
     gene            6547..6678
                     /gene="ctl"
                     /locus_tag="HZT32_RS26035"
                     /old_locus_tag="HZT32_25805"
     CDS             6547..6678
                     /gene="ctl"
                     /locus_tag="HZT32_RS26035"
                     /old_locus_tag="HZT32_25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 lysis protein"
                     /protein_id="WP_072023375.1"
                     /translation="MKITFLIITILSLSGCQANYISDVQGGTVAPSSSSELTGIAVQ"
                     /name="ctl"
     Typing          697..784
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="2.44e-33"
     Typing          52..236
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.237"
                     /coverage="98.0"
                     /evalue="1.02e-80"
     Typing          1443..3037
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.436"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagaactctg ttactaactt ataaatctcg ctctgctaac ctggctacca atggctgctg
       61 ccactggcgt taagtcgtgt cctttccggg ttggacccaa gagaacagtt accggagaag
      121 gcgcagcagt cgggctgaac ggggggttcc tgcatacagc ccggcccgga gcgaactgcc
      181 taagtcgccc cgagatacca gaagacgtga gctatgagaa agcgccacgt gtcccgcaag
      241 ggagaaaggc ggaaaaggta tccggtaatc gatatggcgg gaacgtgggg gcgcaggagg
      301 gagcgattca ccggaaacgg tgatgatctt taagccacgt cgggtttcgc caccgctggc
      361 ggatttcatg gttgagccgt aaggctccca cagatgcacc gaaaaagtgt ctgttttgtg
      421 aagtccgtca ggggggcgga gcctatggaa aaagcgcctg tgccgcggcc tttcccgggg
      481 tcttcgtttt ttgctcacat gttctttccc gggttatccc cggattctgt ggataacggg
      541 gttaccgcct ttgagaaagg ggagtccgct cgccgcagtc gaacgaccga gcgtagcgag
      601 tcagtgagcg aggaagcgga agagcaccgg aagctgcatt ttctccttac gcatctgtgc
      661 ggcatttcac actcggcatg atgcactttt catacaatcc gcactgattc tgcatggtat
      721 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
      781 ctaaaacctg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc tcacaggcaa
      841 gctgtgaccg tctccgggag ctgtatgtgt cagaggtttt caccgttatc cccgaagcgt
      901 gcgaggcagc tgcggtaaag ctcgtcggcg tggtcgtgaa gggcttcgca gatgtccgcc
      961 tggtcgtcgg cgttcagttc attcagtttt tccagcagtg tcagcgtctg gcttctgata
     1021 aagcgggcca tgttaagggc tgttttttcc tgtttagtca cagttctgtt tctccatgtg
     1081 tggggagtct gtcggaggtt tttaatgata ccggtgaaac gcgaaaggat gctcactata
     1141 cgggtcacgg atgaggaaca tgcgcggctg ctggcacgtt gtgagggaaa acaacttgcg
     1201 tcatggatgc gtaaggtctg cctgggtgca ccgccatcga aaacgtcagg tcttccgaca
     1261 ctggcaccgc cgctgttgcg ccagttcgct tcggtcggca ataacctgaa tcagatagcc
     1321 cggaagataa acagcggaca gtggtcgggg catgaccggg ttcatgtggt ggctgcactg
     1381 atggccatcg gacgtgaact gagtgagctt agggatgagg tccgtaaaca gggagaacgg
     1441 gatgatagtt aagtttcatg ccaggggaaa aggcggtggc agtggtccag ttgattacct
     1501 gctgggccgg gagcgtaacc gggaaggggc gagagttctg cggggtgctc ccgaagaggt
     1561 acgggaactc atcgatgcca cgccgtttgc gaaaaaatac acctcggggg ttctgtcttt
     1621 tgcagagcag accctgccac cgggagagcg tgaaagggtg atggagagct ttgagcgggt
     1681 actgatgccc ggtcttgaaa aaaatcagta cagcatcctg tgggtggagc accaggacaa
     1741 ggggcgtctt gagctgaatt ttgtcatccc gaatatggag ctggcgagcg gaaaacggct
     1801 ccagccctat tacgaccggg cggaccgtcc gcgaatcaat gcctggcaga cactggtgaa
     1861 tcaccattac gggctgcacg acccgaacgc accggagaac aggcggacac ttgtcacccc
     1921 gaataatctg ccgaaagcga aacaggaggc cgcagaagcg attacgaggg gactggaggc
     1981 tctttaccat gccggagcgc taaaaacgcg tcaggatgtc actgaggcgc ttacagcggc
     2041 aggttttgag gttgtgagga caaccaggag cagtatcagt atcgccgacc cggagggcgg
     2101 gcgtaatctc aggctgaaag gggcgctgta tgagcaatct tttacaaatg gggacagact
     2161 tcgagaagaa actgaaagag cgtgccgtct ctacagagaa catgctgaac agcgagttca
     2221 ggaagctcga agcgtctgta gacagggaac tgaacttaaa cgggcagaaa atcagagacg
     2281 ctatcagcgc acacacagca gcggtgaaag aacaactgga gacgctgaac agtgccgtga
     2341 acgcgcagtt cagcgccaca gagaacgaac tggcccggcg gcaggaagag ctgctctgga
     2401 agctgatcaa agggcggata ctgtacccgt cgctgacggc gctttgcgtc acgggcggga
     2461 tttttctggc gtcatggggg ctgattcagt ggcaggaaag ccggattgcg gcgaacatcc
     2521 tggctatcag ggaccaggaa gagacgctgg cgaaactccg ggaaaaaaca tggggtgtga
     2581 cgtatcacga gggcaggaac gggaaatttc tggtgctgcc gtccggggtg aagggggaaa
     2641 acaactggac ggtggagaag aagaacgcag taagactggt cagggagtaa gtgagtatga
     2701 cagagctgga aatgcactta ctgaacgcct tagagcaatt gcagcaggac tatacgcagc
     2761 ggctgaaaga ctgggagagc gccttcacgg atttgcagca tatgttcgag ctgacacgac
     2821 aggagaacgg gcaactgagg gaacagtgcg acgacttgag tcagcaggtg cagagattag
     2881 ccgggcaggt ggaccgcttg agtcggttat ttatcacgaa cagcagataa gggatgaaca
     2941 ggaaaggcag gcacgcctgg aacgggagcg tgcgcggtcc tggcatgaag agcaggaacg
     3001 ggaagcacgg gtatggcgtg gcccgtcaat ggggatgtga cggtgagttg ttccgggagg
     3061 ttgccagatg gtaccggaac gcgttaaaaa cgcatccggg cgcgattctg gagtgtcgtt
     3121 tttgttgctg tgttttgcct ctttgccaga tcgcgctcat tcctcagcct catcccgcga
     3181 tgtctgcgta tgtcatcaac tgaaaagatg gaaaaaaaac agtgtcatga ggttaacgca
     3241 ggcaaaacca gcgttattca catggctgaa tagctatgca tacggttaaa atttatcagg
     3301 tgcgatcgcg gcagcttttc gggtggtttg ttgccatttt tacctgtcag cagccgtgat
     3361 cgcgctgaac gcatttcagc ggtgcgtaca attaagggat tatggtaaat cccttactgt
     3421 ctgccctcgt atccatcgag ataaaccgca gaaatggtgt ctgccattag tttcattttt
     3481 ttcggcgaag gcatatcgct gtttgtgtat tttctgccag caaaggttgt tgtgctccgg
     3541 agtgagtgaa aaaatttttc tgagtggaat tcttttacac gttgctgtgc cgtttctgct
     3601 gatactccgg ctgctttgtc tttcatcccc atatcccgga actgctgaaa gaacgggagc
     3661 atcatacgta tggtgtctgc tttctgggtg tagaagttag gatcaaatcc atcatctgaa
     3721 gcataaacat aatgccgggt atgtcgttcc aggctggtct ttggggtgca tcctgtaaga
     3781 gctattgttg ctgttatcag aggtatcaga agcagcctgg cagcggcaga tctcataaac
     3841 atctccttgc gtttttaatc ttgatactca gggagttata tatccgaaga gtatctctta
     3901 tcatacgtcg gtttactccc aatgtgattt gatggtcaat gagtcaggaa taatcccgtg
     3961 aaaaaatgtg tgccagtcct gctttttagt gttctttctg cttcggttgc cagtcatgcg
     4021 gacagcaatg tttttcaaca aaaggccgag aagacaaaaa acggcattgt tgcggatgtt
     4081 aatgccagac agaaaatgtt cagtaacaga ctgaagatac aacttcagat cgatgagttg
     4141 agagcagagc tggctggcac cagagatgcc caaaaaagag aggatttgcg taaaaaactg
     4201 gagtctctgg agcggcaaaa gaaatcctta cgctgatggg ggcgtatttt ccccggtttc
     4261 ttccataacc tttttaccgt tcagatattt acgggcgagg cgaatgcgtc ttgcctggta
     4321 taccgccgag taagcctggg tgggattcac accattactg atttcactga ccacatgacg
     4381 ctgatctgcc agtgtcagcg tgtaaacctg tttctggatg aggtcacggg ctttgtcatg
     4441 ctcatatccg gcagcaacaa gggtgtcgag aagtcgtttg tctgttttgt tcagtttatg
     4501 gcggggtgat ttgctcacat cgttctccgt tttttcatga tccaacagtt ttatatctca
     4561 tcctgatttg ctccggtgtt ctgtttcgac gttctcaatg cctgcctccc catagtgtga
     4621 tccttattgc aaaaatgaaa atccatgctc ttgacatgga caatgctgag tagtaggttt
     4681 ttactgtaca taaaaccagt ggttatatgt acagtatgtt gtttttaatt tattgtttta
     4741 attatcaaag aggaatttct ttatggataa agtcactgat aattctccag atgtggagag
     4801 cacagaatct actgaggggt cattcccaac tgttggggtt gatactggcg atacgattac
     4861 agcgacgctt gcaactggaa ctgaaaatgt tggtggaggc ggtggagcat ttggtggggc
     4921 cagtgaaagt tctgctgcga tacatgcaac cgctaaatgg tctaccgcgc agttgaaaaa
     4981 acatcaggct gaacaggctg cccgtgctgc tgcggctgag gcagcattgg caaaagcgaa
     5041 atctcagcgt gatgccctga ctcaacgtct caaggatatt gttaatgacg ctttacgtgc
     5101 taatgccgct cgtagtccat cagtaactga ccttgctcat gccaataata tggcaatgca
     5161 ggcagaggct gagcgtttgc gccttgcgaa ggcagagcaa aaagcccgtg aagaagctga
     5221 agcagcagaa aaagcgctcc gggaagcaga acgccaacgt gatgagattg cccgccaaca
     5281 ggctgaaacc gcgcatttgt tagcaatggc ggaggcagca gaggctgaga aaaatcgaca
     5341 ggattctctt gatgaagagc atcgggctgt ggaagtggca gagaagaagc tggctgaggc
     5401 taaagctgaa ctggcgaagg ccgaaagcga tgtacagagt aagcaagcga ttgtttccag
     5461 agttgcaggg gagcttgaaa acgctcaaaa aagtgttgat gtgaaggtta ccggatttcc
     5521 tggatggcgt gatgttcaga aaaaactgga gagacaattg caggataaga agaatgaata
     5581 ttcgtcagtg acgaatgctc ttaattctgc tgttagcatt agagatgcta aaaaaacaga
     5641 agttcagaat gctgagataa aattaaaaga agctaaggat gctcttgaga agagtcaggt
     5701 aaaagactct gttgatacta tggttgggtt ttatcaatat ataaccgaac aatatgggga
     5761 aaaatattcc agaatagctc aggatttagc tgaaaaggcg aagggtagta aatttaatag
     5821 tgttgatgaa gcacttgctg catttgaaaa gtataaaaat gtactggata agaaattcag
     5881 taaggttgat agggatgata tttttaatgc tttagagtct attacttatg atgagtgggc
     5941 caagcatcta gaaaagatct ctagagctct taaggttact ggatatttgt ctttcgggca
     6001 tgatgtatgg gatggtaccc taaagggatt aaaaacagga gactggaagc ctttatttgt
     6061 cactctggag aagagcgcgg tagatttcgg cgtggcaaaa attgtggcat taatgtttag
     6121 ttttattgtt ggtgcgcctc ttggcttctg gggaattgca attatcacag gtattgtttc
     6181 ttcttacata ggggatgatg agttgaacaa gcttaatgaa ttgctaggta tttaatttct
     6241 ctttagagag taatatatga aactgggcaa tcctactggg attgccattg ctaggtaaag
     6301 aaaataaaaa aggttgtatg agtaccaagg ttcttttttc atttttgtcg tgttttgtat
     6361 aaaagaaaat atagttagtt ttgaatatgg gtatagtaat acatgtatag atgcgatgaa
     6421 tatgaaaagt gaaaatgggg gcgtcttata gtttaataac aaatagaata gccatgggat
     6481 aagtgactct agtaaattgt gtaggtagta ttttacggtc attatttatg ttgttatagg
     6541 ggtagaatga aaataacgtt tttgataatt acaatattat ccttgtcggg atgtcaagca
     6601 aattatatta gtgatgtaca aggagggacg gtcgctccat cttcctcttc tgaactgaca
     6661 gggatcgcgg ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6721 taatctgctg cttgcaaaca aaaaaaccac cgctaccaac ggtggtttgt ttgccggatc
     6781 aagatctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag atacccaaaa
     6841 cttgtccttc tagtgtagtc aaaatcaggc tgatacttc
//
