LOCUS       NZ_CP058864             4201 bp    DNA     circular CON 03-APR-2024
DEFINITION  Klebsiella quasipneumoniae strain STLEFF_28 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP058864
VERSION     NZ_CP058864.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925557
            Assembly: GCF_022494055.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Che,Y.
  TITLE     Complete genomes of MDR isolates collected from Hong Kong wastewater
            treatment plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. Unicycler 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494055.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 06:17:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,561
            CDSs (total)                      :: 5,438
            Genes (coding)                    :: 5,267
            CDSs (with protein)               :: 5,267
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 61 of 171
            Pseudo Genes (incomplete)         :: 115 of 171
            Pseudo Genes (internal stop)      :: 35 of 171
            Pseudo Genes (multiple problems)  :: 31 of 171
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058864.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="STLEFF_28"
                     /isolation_source="Stanley WWTPs_28 WWTPs effluent"
                     /host="WWTPs effluent"
                     /db_xref="taxon:1463165"
                     /plasmid="unnamed5"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.2 N 114.2 E"
                     /collection_date="2018-12"
     gene            complement(308..499)
                     /locus_tag="HZT23_RS27700"
                     /old_locus_tag="HZT23_27575"
     CDS             complement(308..499)
                     /locus_tag="HZT23_RS27700"
                     /old_locus_tag="HZT23_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            918..1031
                     /locus_tag="HZT23_RS27705"
                     /old_locus_tag="HZT23_27580"
     CDS             918..1031
                     /locus_tag="HZT23_RS27705"
                     /old_locus_tag="HZT23_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(1405..2515)
                     /locus_tag="HZT23_RS27710"
                     /old_locus_tag="HZT23_27585"
                     /pseudo=""
     CDS             complement(1405..2515)
                     /locus_tag="HZT23_RS27710"
                     /old_locus_tag="HZT23_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            2757..2948
                     /locus_tag="HZT23_RS27715"
                     /old_locus_tag="HZT23_27590"
     CDS             2757..2948
                     /locus_tag="HZT23_RS27715"
                     /old_locus_tag="HZT23_27590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049106033.1"
                     /translation="MLKKDDIIFMKSCGNAKTIIFLRGVDEKLMINASFEEIKFAINNN
                     LQELPGVQAVAATLKIAT"
                     /name="hypothetical protein"
     gene            3080..3400
                     /locus_tag="HZT23_RS27720"
                     /old_locus_tag="HZT23_27595"
     CDS             3080..3400
                     /locus_tag="HZT23_RS27720"
                     /old_locus_tag="HZT23_27595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202963179.1"
                     /translation="MMRVLSLGAILMAMSALPLTAISQVTLAPDGSYVSGSEATLAPDG
                     SYVGGSEVTLAPDGSYVGGSEVTLAPDGSYVGGSEVTLAPDGSYVGGSEATLAPDGSYV
                     GN"
                     /name="hypothetical protein"
     gene            3603..3719
                     /locus_tag="HZT23_RS27725"
                     /old_locus_tag="HZT23_27600"
     CDS             3603..3719
                     /locus_tag="HZT23_RS27725"
                     /old_locus_tag="HZT23_27600"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_240709524.1"
                     /translation="MDFVLLAIAIALLVVAVYSGISYFREKKTISRTFRRRR"
                     /name="small membrane protein"
     Typing          375..994
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.929"
                     /coverage="99.0"
                     /evalue="2.55e-171"
ORIGIN
        1 aatgtaattc tgtagtcagg aagaataagc cgttcatgca ggcatgaacg atacagtgag
       61 gaacgctttt tttgtcacga tctcccctta acctgcgtaa atcacccgcc atggttgatt
      121 atttttcaac ctcaaaatgg tcatacacct aacgcagctg cgattttcat cgcaacttca
      181 tgaaaaataa ataaaaaaga aaaaatacag ggattgtggg tattaccagg gtaataatgt
      241 taagcgctaa gacaatgaaa attaaggcga tgccggtatt actccggtaa tacccagaac
      301 ggagacatta cgcaccgtcc tgctgctcat tcaggcgcgc tgacaggctt cggtaaaggc
      361 gttccgcatc ctcatggagt ttctcgcaga aatcagcctc atgatccaga tccagctcat
      421 taagcctgtc cagaagaagc agggactgat cctggatgaa tttagccatg ttaacggctg
      481 tcttttgttg tttgttcatg aaggccccgg cgcgaaagtg cttactgtac gttctgattg
      541 ttttccgctt cctcgctcat tcgctcactc cgttcggtca tgagtctgcg gcgagcggag
      601 ggaagataag cagataacct aacggtaagc ataacgaaag ctaaggacaa gggcgaaaag
      661 gccacgcccc gccggggcgt ttttccatag gctccgcccc cctgacaagc atcacgaaat
      721 ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      781 ccctggtggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc
      841 gctgttatgg ccgcgtttat ctcattccac gcctgacact cagttccggg taggcagttc
      901 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgactgctgc gccttatccg
      961 gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcgccactg gcagcagcca
     1021 ctggtaactg atttagagga gatagtcttg aagtcatgcg ccagttaagg ctaaactaaa
     1081 cgaactgttt tggtgactgc gctcctctaa ctagccagtt acctcggttc aaagagttgc
     1141 tgctctgaga accttcgaga aaaccgcctt gctgggcggt tttttcgttt actgagcaag
     1201 agattacgat gctaaagaaa ggatctcaag aggatcctgt tccatatgcc tacccctgaa
     1261 gtgaaaccag caccactccg cgacaccaac catcaaaata aaactattta acccattgtt
     1321 tctgataaga aaaaaaacat atataatcat gatccacaat catccaacgc ggttgacctg
     1381 cccccccagg attagataca accttcagtt agtaatgtcg gttcgttttt cttcatattt
     1441 cccgtttcgc cagcccgccg caaattcagc cggagtctgg tagttcagcg atgaatgtgg
     1501 acgacactcg ctctaatcct gccgccagtc attaatcgtt ttcctggcgt gaacgatatc
     1561 gctgaaccag tgctcattga ggcactcatc cctgaatcgt ccgttaaaac tttcaataaa
     1621 tccgttctgt gttggcctgc ccggctggat aagccgtagc tccaccccat gctcataagc
     1681 ccactggtcg agtgctctgc aggtaaactc cggcccctgg tcagttctta tcgtcgccgg
     1741 atagccgcga aacagtgcaa tgctgtccag aattcgcgtg acctgaacgc ctgaaatccc
     1801 gaagacggcg gtaattgtca gacactcctt cgtgaagtcg tccacgcagg tcaggcactt
     1861 gatcctgcga ccggtggcca gcgcgtccat gacaaaatcc atcggccagg tcaggttggg
     1921 cgcctccggg cggagcagcg gcagacgttc tgttgccaga cctttacgac gtcgtctgcg
     1981 ttttacaccc agcccgttaa ggtggtaaag acggtacacg cgcttgtgat taacatgaag
     2041 gccttcacgg cgcagtaact gccagatgcg tcggtagcca aaacgcctgc gctccagtgc
     2101 cagctcagtg ataaatgcgc atcagccgcc gaacgctgag cctcatagcg gcaggtcgac
     2161 aggggcaaac ctgtaagcct gcaggcacta cgttgcgaca gaccggtcgc atcacacata
     2221 aactcaacgg cttcctgctt ctggtctgtc gtcagtactt tcgccccaga gccacctgaa
     2281 gcgcctgctt atccagcatg gcttcggcaa gcagtttctt gagtctggcg ttctcttcct
     2341 caagcgactt aaggcgctta acctcgggca cctccatacc gccatacttc ttacgccagg
     2401 tgtaaaagat ggcgtcggaa atggcgtgct tacggcagag ctcacgggca gaaaccccgg
     2461 cttcggcctc gcggagaata ctgatgatct gttcgtcgga aaaacgcttc ttcatgagga
     2521 tgtcctcatg tggcttatga agacattact aacatcgcgg tgtattaatc aacggggagc
     2581 aggtcagctc cgctccccgt gatttgttat atacccacca cgggggcatt agttttttta
     2641 ttttttttga tgatatgtac aaaatcagtg gttaggaata atcttaacag ccttaacaaa
     2701 aacacacaaa aacagtggct atgctttatc tcactacact aagtaacgac agtatcatgc
     2761 taaaaaaaga tgatattatc tttatgaaat catgtggtaa tgcaaaaaca ataatatttt
     2821 tacgtggcgt ggatgagaag ctcatgatta acgcgagctt tgaagagata aagtttgcta
     2881 tcaataataa tcttcaggag cttccggggg tacaagcagt agccgcgaca ctgaaaatag
     2941 caacctgatt tcgagtaagg gcactcaaaa tcaagagatc aatttactat aacatcccga
     3001 tcacgaatcc cgcggctgga accggcgttc ggcaccgctg aacgattcaa ttctattttt
     3061 gatattaaca ggagtaatag tgatgagagt attatctctt ggtgccattt taatggctat
     3121 gtcagcatta ccactaaccg cgatatccca agtaacctta gcacctgatg gatcgtacgt
     3181 tagtggttcg gaagcaacct tagcacctga tggctcatac gttggtggtt cggaagtaac
     3241 cttagcacct gatggctcat acgttggtgg ttcggaagta accttagcac ctgatggctc
     3301 atacgttggt ggttcggaag taaccttagc acctgatggc tcatacgttg gtggttcgga
     3361 agcaacctta gcacctgatg gctcatacgt tggaaattaa ccccccatta ttatgctgcg
     3421 taccgatata ctgaattccg aatgccagca tttttggatt tatcataacg tcttcttacg
     3481 tgtactgaac aatgcgtcct caggaaccat tagctcaaag caataagtgt aatgaaaaaa
     3541 gaatgaaaac cacttacaat aactaatcat aacaggtttt ttattaaatt aatcaggtac
     3601 ttatggactt tgttttactt gctatcgcca ttgctctact tgttgtagct gtctactcag
     3661 gaatctcata cttcagagaa aaaaaaacaa tcagtcgtac atttaggcgt aggcgatagc
     3721 gccctattta ccataatccc ttcttacgcg caccgcgaaa acggcctcag ggcgtttctg
     3781 acgggaaacc ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa cgcgcaggct
     3841 ctgtttttac ggcatgcatg agtatgcgcg caagtgcata gcgcggaaat tcacgcaact
     3901 cagccggggt aagtcctgtt tccggcattc ttcccacccg aaaacggtga acgggaacgc
     3961 aatttattgc gtggccgtga ggcgttgccg gcacagccgg aaaaaccgtt ttcagcacgt
     4021 tcaggaaggg ggtcggatgt acaaatggca cagaggatgg aactgacgta aaagtgacgt
     4081 atgagctgaa ggaatgatgt aaaagtgatg caaaagtgtt gtaaaagtga tgtataggct
     4141 gaaggaatga tgcaaaagtg atgtaaaagt gttgtaaaag tgatgtatga actgaaggag
     4201 c
//
