LOCUS       NZ_CP058843             6268 bp    DNA     circular CON 30-APR-2024
DEFINITION  Shigella flexneri strain STLEFF_34 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP058843
VERSION     NZ_CP058843.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925560
            Assembly: GCF_022494115.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. Unicycler 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494115.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 04:20:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,960
            CDSs (total)                      :: 4,839
            Genes (coding)                    :: 4,599
            CDSs (with protein)               :: 4,599
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 77 of 240
            Pseudo Genes (incomplete)         :: 162 of 240
            Pseudo Genes (internal stop)      :: 45 of 240
            Pseudo Genes (multiple problems)  :: 44 of 240
            Pseudo Genes (short protein)      :: 4 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058843.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /strain="STLEFF_34"
                     /isolation_source="Stanley WWTPs_34 WWTPs effluent"
                     /db_xref="taxon:623"
                     /plasmid="unnamed4"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.2 N 114.2 E"
                     /collection_date="2018-12"
     gene            complement(587..778)
                     /locus_tag="HZT26_RS24590"
                     /old_locus_tag="HZT26_24390"
     CDS             complement(587..778)
                     /locus_tag="HZT26_RS24590"
                     /old_locus_tag="HZT26_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            842..1165
                     /locus_tag="HZT26_RS24595"
                     /old_locus_tag="HZT26_24395"
     CDS             842..1165
                     /locus_tag="HZT26_RS24595"
                     /old_locus_tag="HZT26_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001749507.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1374..>1805
                     /locus_tag="HZT26_RS24600"
                     /old_locus_tag="HZT26_24400"
                     /pseudo=""
     CDS             1374..>1805
                     /locus_tag="HZT26_RS24600"
                     /old_locus_tag="HZT26_24400"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1843..2361
                     /locus_tag="HZT26_RS24605"
                     /old_locus_tag="HZT26_24405"
     CDS             1843..2361
                     /locus_tag="HZT26_RS24605"
                     /old_locus_tag="HZT26_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001749510.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            2368..2601
                     /locus_tag="HZT26_RS24610"
                     /old_locus_tag="HZT26_24410"
     CDS             2368..2601
                     /locus_tag="HZT26_RS24610"
                     /old_locus_tag="HZT26_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3084..3527
                     /locus_tag="HZT26_RS24615"
                     /old_locus_tag="HZT26_24415"
     CDS             3084..3527
                     /locus_tag="HZT26_RS24615"
                     /old_locus_tag="HZT26_24415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001605388.1"
                     /translation="MTSGIFLSREDLMLLSDQAKEEVLALATGDSVSGVYEESEDGPTD
                     LSSLQAERLVRGLSDKSRTVLKAILESEDGERGFWCKSLAKELGFDVSELSGVWSGLTR
                     RTRTVSDDSEAYLISWAWDDDRDDYYGTLHPTTYRNCKKAMNF"
                     /name="hypothetical protein"
     gene            complement(3666..4805)
                     /gene="dcm"
                     /locus_tag="HZT26_RS24620"
                     /old_locus_tag="HZT26_24420"
     CDS             complement(3666..4805)
                     /gene="dcm"
                     /locus_tag="HZT26_RS24620"
                     /old_locus_tag="HZT26_24420"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131511.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="WP_000131512.1"
                     /translation="MTDNIAATIKGKRERLHMTQKEFADALGLSKYGDRTIRRWERGET
                     KPTGAELKAVMDFPDTPPYPNNENGRYRMIDLFAGIGGTRLGFHQTNAVNVVFSSEWDK
                     FAQKTYHANYGDFPDGDITKIDEKDIPDHEILVGGFPCVAFSQAGLKKGFNDTRGTLFF
                     DIARIIKEKKPHAFLLENVKNLLGHDKGRTFSIIKNTLEELNYTVYYNIFAAKDFGVPQ
                     NRERIYIVGFNKEKVRNHEHFTFPTPLKTKTRVGDILEKSVDNKYTLSDALWNGHQRRK
                     LVNAAAGKGFGYGLFNENSPYTNTISARYYKDGSEILIEQKGSNPRKITPREASRLQGF
                     PSDFIIPVSDTQAYKQFGNSVAVPVINAIAEKIISTLDS"
                     /name="dcm"
     gene            4876..5832
                     /locus_tag="HZT26_RS24625"
                     /old_locus_tag="HZT26_24425"
     CDS             4876..5832
                     /locus_tag="HZT26_RS24625"
                     /old_locus_tag="HZT26_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640333.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="WP_224985182.1"
                     /translation="MKKLSSRYKRLFFMQRLSPGEFKTLISKERKSHFITPFALVYKTF
                     CDLGYDQKNSDYFLNNPSEYIIAMRKNCWKEFEPFEKEFTTRMLSYLIDEERIKDMSPY
                     DAIRDFTMEYPTHIYDLALSNTQSRRSRAGKEFESILELLMMGAGIPVDVQGAIGKSFF
                     QKNQIGKLVDLVMPGVVQYTSNKRNTMLISAKTTLRERWQEVPEEVNRTGIREMYLATL
                     DDSFSEETINILYEANVVVVTTIENKNFKYKNNNRVLTFEDMLQSAMELSRKWNNVSYT
                     DSEKEEIQQSILKQIEKYSDFPYVVNYYRNRLSALVD"
                     /name="type II restriction endonuclease"
     gene            complement(6024..6185)
                     /locus_tag="HZT26_RS24890"
     CDS             complement(6024..6185)
                     /locus_tag="HZT26_RS24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596883.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRARFI
                     QMLTEFLK"
                     /name="replication initiation protein"
     Typing          417..502
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="3.99e-36"
     Typing          complement(2..395)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.703"
                     /coverage="100.0"
                     /evalue="2.27e-166"
     Typing          complement(5845..6218)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.463"
                     /coverage="89.0"
                     /evalue="1.12e-89"
     Typing          813..1159
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.103"
                     /coverage="98.0"
                     /evalue="1.36e-63"
     Typing          1156..2705
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.802"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
       61 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      121 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatgggaa aacgccagcg
      181 gcgcggcctt ttgttgccct ttgccttttt actcacatgt tctttccggc cttatcccct
      241 gattctgtgg ataaccgtat ttccgccttt gagtgagccg gcaccgcttg ccgcagccga
      301 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt
      361 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
      421 actgatgccg catggttaag ccagtataca ctccgctatc gctacgtgac tgggtcaggg
      481 ctgcgccccg acacccccta aaacctgctg acgcgccctg acgggcttgt ctgctcccgg
      541 catccgctca cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
      601 cgtcatcccc gaaacgtgcg aggcagctgc ggtaaagctc atcggcgtgg tcgtgaagcg
      661 attcacaaat atcggcctgt tcatccgcgt ccagttcgtt aagcttttcc agcagcgtta
      721 atgtctggct tctgataaag cgcgccatgt taagggctgt tttttcctgt ttagtcactg
      781 atgcctccgt gtaaggggga tttctgttca tggggtaatg ataccgatga aacgcgagag
      841 gatgctcacg atacgggtta ctgatgatga acatgccagg ttactggaac gctgtgaggg
      901 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
      961 gaagctgccg acactggcac cgccgttact gcgtcagctg gctgccatcg ggaataacct
     1021 gaatcagacg gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1081 ggtggccgca ctgatggcca tcgagagaga gttgcgcagc ttacggcagg tcgtcaggga
     1141 acagggagcg cgggatgatt gttaaatttc atgccagggg aaaaggcggt ggcagtggtc
     1201 cggttgatta cctgctggga agggagcgaa accgggaagg cgcaacggtg cttcagggta
     1261 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtcgg
     1321 gtgttctgtc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaag gtgatggcga
     1381 gttttgagcg tgtactgatg cccggtctcg aaaagaatca gtacagcatc ctgtgggtgg
     1441 agcaccagga caagggacgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1501 ccggaaaacg cctccagccg tactacgacc gggcagacag gccgagaatt gatgcctggc
     1561 agacgctggt gaatcaccat tacggactgc atgaccccaa cgccccggag aaccgccgga
     1621 cgctgaccct tcctgataac ctgccggaaa cgaaacaggc acttgctgag agcgttacac
     1681 gcggtataga cgcactttac catgccggag agataaaagg ccgtcaggat gtgattcagg
     1741 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     1801 acccgaacgg cgggaggaat atcagactga aaggagcgtt ttatgagcaa tcttttacag
     1861 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     1921 aacgacgagt tcaggaagct cggcgagtct gtcagcgagg ctgtgacctc aaacgagacg
     1981 aaaatcagag acgctatagc cctgttcaca gcctcgacag agaaatcgct ggaaaaacac
     2041 cgggaagggg tgaaagaggc gatgatgcag caccggaagg acgtattaaa gctggcaggg
     2101 aatacgggca tgatgttact ggggatagtc tttctcctgt ttaccgcgag tggcgggacg
     2161 ctctggtatc ttggcggacg gatacagacg aacctggaag aaatcaggaa gcaggaagag
     2221 acattgcaga aactgaacgc gaagacatgg ggcgtggagt ttgtgcagga cgggaacagg
     2281 aaattccttg tccttccgta cgggaaatca gcggaggtga ttccctttca ggggaaagag
     2341 tgggtacatc tgaaggagtg acacagagtg acagagctgg aaatacattt gctgaacgcc
     2401 ttagagcagc tgcaacagga ctatatgcag cggctgagcg aatgggagag cgccttcgtg
     2461 gaattgcaga agatgttttc gcttacgcaa cgggacaacg cgatgctgaa cgagcgggtc
     2521 atgcagttga gtcagcaggt gcagcacttg agcgagcaga cagaacgctt gagccagtta
     2581 tacagcgaga actggagata agagaggcac ggctgataca ggagcgcgaa catgcgttat
     2641 ccctggaacg ggagcgtcag ccggaaatac aggaacgcac gctggatggt ccttcgctgg
     2701 gatggtgaaa tcatgaaaaa tggcagcttc agtggattaa gaggggttaa tgtggcctgt
     2761 accatctggt tgcataggta ttcatgcggt taaaatttat caggcgcgat cgcggcagtt
     2821 tttcgggtga tttgttgcca tttttacctg tctgctgccg tgatcgcgct gaacgcgttt
     2881 tagcggtgcg tgtaatgcga cgttatggta aatccgggct gttgagtgtt gacctctcac
     2941 tatcgcaacc cagttaatgc ggtaaatcaa acgcaactag ctgttaattg atgttagttg
     3001 tattgacttg tcacttgtct tgttgacaac tatcttgtct atgtttattc tccttttatc
     3061 ttaacaagac aatggagtgt ttcatgacta gtggtatttt cttatcccgt gaagatttga
     3121 tgttgttatc agaccaagca aaagaagaag tgctggcatt ggctactggg gattcggttt
     3181 ctggtgttta tgaggagtcg gaagatgggc ctactgatct ttcctcgctt caggcagaaa
     3241 ggcttgttcg tggtttaagt gataagtcgc gaacagtgct gaaagctatt cttgaaagcg
     3301 aggatggtga gcgaggattc tggtgcaaga gcctcgcaaa ggagcttggt tttgatgtct
     3361 ctgagctttc aggtgtctgg tcagggctta cacgcagaac tcgcacggta agtgatgatt
     3421 ctgaggctta tttgattagc tgggcttggg atgatgaccg tgatgattac tatggaacgc
     3481 tacatccaac aacctatcgc aactgcaaga aagctatgaa cttttgattg ttctcgctgt
     3541 gatactttcc ctgtgagccc gccatgtgcg ggcttttttc attggttcgt gtagtgagac
     3601 gttatggtaa atccatatgg ggggggaatt ggattctggg tcgcattgtg tccttatgac
     3661 ttagtttatg aatctaaggt cgatatgatt ttttctgcta ttgcattgat aacagggact
     3721 gcgactgaat tcccaaattg cttataagcc tgtgtatccg atacagggat aataaaatca
     3781 ctaggaaacc cttgaagtct tgacgcttcc cgcggagtta tttttcttgg gttacttcct
     3841 ttttgctcta ttaatatttc gctaccatct ttatagtacc tcgcacttat ggtattagta
     3901 tatgggctat tttcattaaa taaaccatac ccaaagcctt taccggctgc tgcatttaca
     3961 agtttcctgc gttggtgtcc attccagagc gcatctgata gcgtatattt attgtctacg
     4021 ctcttttcaa gaatatctcc aactcttgtt tttgttttta atggggttgg aaaagtaaaa
     4081 tgttcatgat ttcttacttt ttctttatta aagcctacaa tgtatatgcg ttctctattt
     4141 tgtggaacac caaagtcttt tgcagcgaag atattgtaat acacagtgta attcaattct
     4201 tcaagtgtgt tttttattat tgaaaaagtt ctacctttat catgtcctag caagtttttt
     4261 acattttcaa gtaaaaaagc atgaggtttt ttttccttta ttatccttgc tatatcaaaa
     4321 aacaaagttc ctcttgtatc attaaatcct tttttgagcc ctgcttgaga gaatgctacg
     4381 caaggaaacc caccgacaag aatttcatga tcaggaatat ctttttcatc gatttttgtt
     4441 atgtccccat cagggaaatc accataattt gcatgatatg tcttttgagc aaacttatcc
     4501 cattcgctgg aaaaaacaac atttacagcg ttagtttgat gaaatccaag tctggttcca
     4561 ccgattccag caaataaatc gatcatgcga tatctgccat tctcgttgtt tgggtaaggt
     4621 ggtgtgtctg gaaagtccat aactgctttt agttccgccc cagtaggttt tgtttccccg
     4681 cgctcccatc ttcttatggt tctgtctcca tattttgaaa gaccaagggc gtctgcaaac
     4741 tctttttgag tcatatgaag tctttctctt tttcctttga ttgttgctgc gatattatca
     4801 gtcatgcatg tctaccagaa ttaagtgttg gttttaggac aatttgtcct gttttgattc
     4861 caattatgaa cgtgcatgaa aaaactttca agtagatata aaaggttatt ttttatgcaa
     4921 agattgagtc ctggtgaatt taaaactcta atttctaaag agagaaagtc gcattttatt
     4981 acgccatttg ctttggttta taaaacattt tgtgatttag ggtatgatca aaaaaatagt
     5041 gattattttt tgaataatcc aagtgaatat ataattgcaa tgagaaaaaa ctgttggaaa
     5101 gaatttgaac catttgaaaa agaattcaca acaagaatgt tatcatatct tattgatgaa
     5161 gaaagaataa aagatatgag tccttatgat gccattcgtg attttacgat ggaataccct
     5221 acacacatat atgatttggc actgtctaac actcaatcca gacgtagcag ggcaggaaaa
     5281 gagtttgagt ctattcttga gctattaatg atgggggcag gaattcctgt tgatgtgcag
     5341 ggggctattg ggaagtcatt ttttcaaaaa aaccaaatag gtaaacttgt agatcttgtt
     5401 atgccaggtg tagtccagta cacatctaac aaaagaaata caatgttgat ttccgctaaa
     5461 acaacgctga gggagcgatg gcaagaagta cctgaagagg ttaatagaac aggaattaga
     5521 gaaatgtatc tagctacact agatgattct tttagcgagg agacaataaa tatattatac
     5581 gaggctaatg tggtcgttgt aacgacgata gaaaataaaa atttcaaata taaaaataat
     5641 aatagggtgc taacctttga agatatgcta cagtcagcaa tggaattatc aagaaagtgg
     5701 aataatgtaa gttacacaga ctctgaaaaa gaggaaattc aacagtcaat attaaaacaa
     5761 atagaaaaat atagtgattt cccttatgtt gttaattatt atagaaatag gcttagtgct
     5821 cttgttgact aaaaaactaa aaggatctct tgagatcctt tttttctgcg cgtaatcttg
     5881 gacctgtaaa cgaaaaaacc accctggcag gtggtttttt cgaaggttag ctaatcctgg
     5941 cagattatct aaccgaggta atctggcttc agcagagcac agataccaaa tactgtcctt
     6001 ccagtgtagc cgtagttagg ccatcacttc aagaactctg taagcatctg gataaatctc
     6061 gctctgctaa tccggttacc agtggctgct gccagtggcg ttaaggcgtg tcttaccggg
     6121 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6181 tgcacacagc ccagcttgga gcgaacgacc tacaccgggc cgagatacca acagcgtgag
     6241 ctatgagaaa gcgccacgct tcccgaag
//
