LOCUS       NZ_CP058836             6268 bp    DNA     circular CON 30-APR-2024
DEFINITION  Shigella flexneri strain STLEFF_27 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP058836
VERSION     NZ_CP058836.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925556
            Assembly: GCF_022494035.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. Unicycler 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494035.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 04:32:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,914
            CDSs (total)                      :: 4,793
            Genes (coding)                    :: 4,554
            CDSs (with protein)               :: 4,554
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 76 of 239
            Pseudo Genes (incomplete)         :: 163 of 239
            Pseudo Genes (internal stop)      :: 44 of 239
            Pseudo Genes (multiple problems)  :: 44 of 239
            Pseudo Genes (short protein)      :: 4 of 239
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058836.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /strain="STLEFF_27"
                     /isolation_source="Stanley WWTPs_27 WWTPs effluent"
                     /db_xref="taxon:623"
                     /plasmid="unnamed3"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.2 N 114.2 E"
                     /collection_date="2018-12"
     gene            complement(join(6060..6268,1..310))
                     /locus_tag="HZT22_RS24350"
                     /old_locus_tag="HZT22_24195"
     CDS             complement(join(6060..6268,1..310))
                     /locus_tag="HZT22_RS24350"
                     /old_locus_tag="HZT22_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001749510.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<348..998)
                     /locus_tag="HZT22_RS24355"
                     /old_locus_tag="HZT22_24200"
                     /pseudo=""
     CDS             complement(<348..998)
                     /locus_tag="HZT22_RS24355"
                     /old_locus_tag="HZT22_24200"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(988..1311)
                     /locus_tag="HZT22_RS24360"
                     /old_locus_tag="HZT22_24205"
     CDS             complement(988..1311)
                     /locus_tag="HZT22_RS24360"
                     /old_locus_tag="HZT22_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001749507.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1375..1566
                     /locus_tag="HZT22_RS24365"
                     /old_locus_tag="HZT22_24210"
     CDS             1375..1566
                     /locus_tag="HZT22_RS24365"
                     /old_locus_tag="HZT22_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2236..2397
                     /locus_tag="HZT22_RS24650"
     CDS             2236..2397
                     /locus_tag="HZT22_RS24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596883.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRARFI
                     QMLTEFLK"
                     /name="replication initiation protein"
     gene            complement(2589..3545)
                     /locus_tag="HZT22_RS24370"
                     /old_locus_tag="HZT22_24215"
     CDS             complement(2589..3545)
                     /locus_tag="HZT22_RS24370"
                     /old_locus_tag="HZT22_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640333.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="WP_224985182.1"
                     /translation="MKKLSSRYKRLFFMQRLSPGEFKTLISKERKSHFITPFALVYKTF
                     CDLGYDQKNSDYFLNNPSEYIIAMRKNCWKEFEPFEKEFTTRMLSYLIDEERIKDMSPY
                     DAIRDFTMEYPTHIYDLALSNTQSRRSRAGKEFESILELLMMGAGIPVDVQGAIGKSFF
                     QKNQIGKLVDLVMPGVVQYTSNKRNTMLISAKTTLRERWQEVPEEVNRTGIREMYLATL
                     DDSFSEETINILYEANVVVVTTIENKNFKYKNNNRVLTFEDMLQSAMELSRKWNNVSYT
                     DSEKEEIQQSILKQIEKYSDFPYVVNYYRNRLSALVD"
                     /name="type II restriction endonuclease"
     gene            3616..4755
                     /gene="dcm"
                     /locus_tag="HZT22_RS24375"
                     /old_locus_tag="HZT22_24220"
     CDS             3616..4755
                     /gene="dcm"
                     /locus_tag="HZT22_RS24375"
                     /old_locus_tag="HZT22_24220"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131511.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="WP_000131512.1"
                     /translation="MTDNIAATIKGKRERLHMTQKEFADALGLSKYGDRTIRRWERGET
                     KPTGAELKAVMDFPDTPPYPNNENGRYRMIDLFAGIGGTRLGFHQTNAVNVVFSSEWDK
                     FAQKTYHANYGDFPDGDITKIDEKDIPDHEILVGGFPCVAFSQAGLKKGFNDTRGTLFF
                     DIARIIKEKKPHAFLLENVKNLLGHDKGRTFSIIKNTLEELNYTVYYNIFAAKDFGVPQ
                     NRERIYIVGFNKEKVRNHEHFTFPTPLKTKTRVGDILEKSVDNKYTLSDALWNGHQRRK
                     LVNAAAGKGFGYGLFNENSPYTNTISARYYKDGSEILIEQKGSNPRKITPREASRLQGF
                     PSDFIIPVSDTQAYKQFGNSVAVPVINAIAEKIISTLDS"
                     /name="dcm"
     gene            complement(4894..5337)
                     /locus_tag="HZT22_RS24380"
                     /old_locus_tag="HZT22_24225"
     CDS             complement(4894..5337)
                     /locus_tag="HZT22_RS24380"
                     /old_locus_tag="HZT22_24225"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001605388.1"
                     /translation="MTSGIFLSREDLMLLSDQAKEEVLALATGDSVSGVYEESEDGPTD
                     LSSLQAERLVRGLSDKSRTVLKAILESEDGERGFWCKSLAKELGFDVSELSGVWSGLTR
                     RTRTVSDDSEAYLISWAWDDDRDDYYGTLHPTTYRNCKKAMNF"
                     /name="hypothetical protein"
     gene            complement(5820..6053)
                     /locus_tag="HZT22_RS24385"
                     /old_locus_tag="HZT22_24230"
     CDS             complement(5820..6053)
                     /locus_tag="HZT22_RS24385"
                     /old_locus_tag="HZT22_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1652..1737)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="3.99e-36"
     Typing          1759..2308
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.756"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..998)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.687"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(995..1341)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.103"
                     /coverage="98.0"
                     /evalue="1.36e-63"
     Typing          complement(5717..6267)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.241"
                     /coverage="99.0"
                     /evalue="1.36e-82"
ORIGIN
        1 cactcgcggt aaacaggaga aagactatcc ccagtaacat catgcccgta ttccctgcca
       61 gctttaatac gtccttccgg tgctgcatca tcgcctcttt caccccttcc cggtgttttt
      121 ccagcgattt ctctgtcgag gctgtgaaca gggctatagc gtctctgatt ttcgtctcgt
      181 ttgaggtcac agcctcgctg acagactcgc cgagcttcct gaactcgtcg ttcagcattt
      241 tctctgtaga ttcggctctc tctttcagct ttttctcgaa ctccgcgccc gtctgtaaaa
      301 gattgctcat aaaacgctcc tttcagtctg atattcctcc cgccgttcgg gtctgcaatg
      361 ctgatactgc ttcgcgtcac cctgaccact tcaagccccg cctctgtgag cgcctgaatc
      421 acatcctgac ggccttttat ctctccggca tggtaaagtg cgtctatacc gcgtgtaacg
      481 ctctcagcaa gtgcctgttt cgtttccggc aggttatcag gaagggtcag cgtccggcgg
      541 ttctccgggg cgttggggtc atgcagtccg taatggtgat tcaccagcgt ctgccaggca
      601 tcaattctcg gcctgtctgc ccggtcgtag tacggctgga ggcgttttcc ggtctgtagc
      661 tccatgttcg gaatgacaaa attcagctca agccgtccct tgtcctggtg ctccacccac
      721 aggatgctgt actgattctt ttcgagaccg ggcatcagta cacgctcaaa actcgccatc
      781 accttttcac gccctcccgg cggcagctcc ttctccgcga acgacagaac acccgacgtg
      841 tatttcttcg caaatggcgt ggcatcgatg agttcccgga cttcttccgg attaccctga
      901 agcaccgttg cgccttcccg gtttcgctcc cttcccagca ggtaatcaac cggaccactg
      961 ccaccgcctt ttcccctggc atgaaattta acaatcatcc cgcgctccct gttccctgac
     1021 gacctgccgt aagctgcgca actctctctc gatggccatc agtgcggcca ccacctgaac
     1081 ccggtcaccg gaagaccact gcccgctgtt caccttcctc gccgtctgat tcaggttatt
     1141 cccgatggca gccagctgac gcagtaacgg cggtgccagt gtcggcagct tcccggaacg
     1201 ggcaaccggc tcacccaggc agacccgccg catccatacc gccagctgtt taccctcaca
     1261 gcgttccagt aacctggcat gttcatcatc agtaacccgt atcgtgagca tcctctcgcg
     1321 tttcatcggt atcattaccc catgaacaga aatccccctt acacggaggc atcagtgact
     1381 aaacaggaaa aaacagccct taacatggcg cgctttatca gaagccagac attaacgctg
     1441 ctggaaaagc ttaacgaact ggacgcggat gaacaggccg atatttgtga atcgcttcac
     1501 gaccacgccg atgagcttta ccgcagctgc ctcgcacgtt tcggggatga cggtgaaaac
     1561 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtgagcgga tgccgggagc
     1621 agacaagccc gtcagggcgc gtcagcaggt tttagggggt gtcggggcgc agccctgacc
     1681 cagtcacgta gcgatagcgg agtgtatact ggcttaacca tgcggcatca gtgcagattg
     1741 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatgca
     1801 gcttccggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1861 ggcaagcggt gccggctcac tcaaaggcgg aaatacggtt atccacagaa tcaggggata
     1921 aggccggaaa gaacatgtga gtaaaaaggc aaagggcaac aaaaggccgc gccgctggcg
     1981 ttttcccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2041 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2101 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2161 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2221 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2281 aactatcgtc ttgagtccaa cccggtaaga cacgccttaa cgccactggc agcagccact
     2341 ggtaaccgga ttagcagagc gagatttatc cagatgctta cagagttctt gaagtgatgg
     2401 cctaactacg gctacactgg aaggacagta tttggtatct gtgctctgct gaagccagat
     2461 tacctcggtt agataatctg ccaggattag ctaaccttcg aaaaaaccac ctgccagggt
     2521 ggttttttcg tttacaggtc caagattacg cgcagaaaaa aaggatctca agagatcctt
     2581 ttagtttttt agtcaacaag agcactaagc ctatttctat aataattaac aacataaggg
     2641 aaatcactat atttttctat ttgttttaat attgactgtt gaatttcctc tttttcagag
     2701 tctgtgtaac ttacattatt ccactttctt gataattcca ttgctgactg tagcatatct
     2761 tcaaaggtta gcaccctatt attattttta tatttgaaat ttttattttc tatcgtcgtt
     2821 acaacgacca cattagcctc gtataatata tttattgtct cctcgctaaa agaatcatct
     2881 agtgtagcta gatacatttc tctaattcct gttctattaa cctcttcagg tacttcttgc
     2941 catcgctccc tcagcgttgt tttagcggaa atcaacattg tatttctttt gttagatgtg
     3001 tactggacta cacctggcat aacaagatct acaagtttac ctatttggtt tttttgaaaa
     3061 aatgacttcc caatagcccc ctgcacatca acaggaattc ctgcccccat cattaatagc
     3121 tcaagaatag actcaaactc ttttcctgcc ctgctacgtc tggattgagt gttagacagt
     3181 gccaaatcat atatgtgtgt agggtattcc atcgtaaaat cacgaatggc atcataagga
     3241 ctcatatctt ttattctttc ttcatcaata agatatgata acattcttgt tgtgaattct
     3301 ttttcaaatg gttcaaattc tttccaacag ttttttctca ttgcaattat atattcactt
     3361 ggattattca aaaaataatc actatttttt tgatcatacc ctaaatcaca aaatgtttta
     3421 taaaccaaag caaatggcgt aataaaatgc gactttctct ctttagaaat tagagtttta
     3481 aattcaccag gactcaatct ttgcataaaa aataaccttt tatatctact tgaaagtttt
     3541 ttcatgcacg ttcataattg gaatcaaaac aggacaaatt gtcctaaaac caacacttaa
     3601 ttctggtaga catgcatgac tgataatatc gcagcaacaa tcaaaggaaa aagagaaaga
     3661 cttcatatga ctcaaaaaga gtttgcagac gcccttggtc tttcaaaata tggagacaga
     3721 accataagaa gatgggagcg cggggaaaca aaacctactg gggcggaact aaaagcagtt
     3781 atggactttc cagacacacc accttaccca aacaacgaga atggcagata tcgcatgatc
     3841 gatttatttg ctggaatcgg tggaaccaga cttggatttc atcaaactaa cgctgtaaat
     3901 gttgtttttt ccagcgaatg ggataagttt gctcaaaaga catatcatgc aaattatggt
     3961 gatttccctg atggggacat aacaaaaatc gatgaaaaag atattcctga tcatgaaatt
     4021 cttgtcggtg ggtttccttg cgtagcattc tctcaagcag ggctcaaaaa aggatttaat
     4081 gatacaagag gaactttgtt ttttgatata gcaaggataa taaaggaaaa aaaacctcat
     4141 gcttttttac ttgaaaatgt aaaaaacttg ctaggacatg ataaaggtag aactttttca
     4201 ataataaaaa acacacttga agaattgaat tacactgtgt attacaatat cttcgctgca
     4261 aaagactttg gtgttccaca aaatagagaa cgcatataca ttgtaggctt taataaagaa
     4321 aaagtaagaa atcatgaaca ttttactttt ccaaccccat taaaaacaaa aacaagagtt
     4381 ggagatattc ttgaaaagag cgtagacaat aaatatacgc tatcagatgc gctctggaat
     4441 ggacaccaac gcaggaaact tgtaaatgca gcagccggta aaggctttgg gtatggttta
     4501 tttaatgaaa atagcccata tactaatacc ataagtgcga ggtactataa agatggtagc
     4561 gaaatattaa tagagcaaaa aggaagtaac ccaagaaaaa taactccgcg ggaagcgtca
     4621 agacttcaag ggtttcctag tgattttatt atccctgtat cggatacaca ggcttataag
     4681 caatttggga attcagtcgc agtccctgtt atcaatgcaa tagcagaaaa aatcatatcg
     4741 accttagatt cataaactaa gtcataagga cacaatgcga cccagaatcc aattcccccc
     4801 ccatatggat ttaccataac gtctcactac acgaaccaat gaaaaaagcc cgcacatggc
     4861 gggctcacag ggaaagtatc acagcgagaa caatcaaaag ttcatagctt tcttgcagtt
     4921 gcgataggtt gttggatgta gcgttccata gtaatcatca cggtcatcat cccaagccca
     4981 gctaatcaaa taagcctcag aatcatcact taccgtgcga gttctgcgtg taagccctga
     5041 ccagacacct gaaagctcag agacatcaaa accaagctcc tttgcgaggc tcttgcacca
     5101 gaatcctcgc tcaccatcct cgctttcaag aatagctttc agcactgttc gcgacttatc
     5161 acttaaacca cgaacaagcc tttctgcctg aagcgaggaa agatcagtag gcccatcttc
     5221 cgactcctca taaacaccag aaaccgaatc cccagtagcc aatgccagca cttcttcttt
     5281 tgcttggtct gataacaaca tcaaatcttc acgggataag aaaataccac tagtcatgaa
     5341 acactccatt gtcttgttaa gataaaagga gaataaacat agacaagata gttgtcaaca
     5401 agacaagtga caagtcaata caactaacat caattaacag ctagttgcgt ttgatttacc
     5461 gcattaactg ggttgcgata gtgagaggtc aacactcaac agcccggatt taccataacg
     5521 tcgcattaca cgcaccgcta aaacgcgttc agcgcgatca cggcagcaga caggtaaaaa
     5581 tggcaacaaa tcacccgaaa aactgccgcg atcgcgcctg ataaatttta accgcatgaa
     5641 tacctatgca accagatggt acaggccaca ttaacccctc ttaatccact gaagctgcca
     5701 tttttcatga tttcaccatc ccagcgaagg accatccagc gtgcgttcct gtatttccgg
     5761 ctgacgctcc cgttccaggg ataacgcatg ttcgcgctcc tgtatcagcc gtgcctctct
     5821 tatctccagt tctcgctgta taactggctc aagcgttctg tctgctcgct caagtgctgc
     5881 acctgctgac tcaactgcat gacccgctcg ttcagcatcg cgttgtcccg ttgcgtaagc
     5941 gaaaacatct tctgcaattc cacgaaggcg ctctcccatt cgctcagccg ctgcatatag
     6001 tcctgttgca gctgctctaa ggcgttcagc aaatgtattt ccagctctgt cactctgtgt
     6061 cactccttca gatgtaccca ctctttcccc tgaaagggaa tcacctccgc tgatttcccg
     6121 tacggaagga caaggaattt cctgttcccg tcctgcacaa actccacgcc ccatgtcttc
     6181 gcgttcagtt tctgcaatgt ctcttcctgc ttcctgattt cttccaggtt cgtctgtatc
     6241 cgtccgccaa gataccagag cgtcccgc
//
