LOCUS       NZ_CP058792            19857 bp    DNA     circular CON 02-MAY-2024
DEFINITION  Shigella flexneri strain STLIN_6 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP058792
VERSION     NZ_CP058792.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925576
            Assembly: GCF_022494435.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19857)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19857)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. Unicycler 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494435.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 05:11:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,890
            CDSs (total)                      :: 4,767
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 65 of 209
            Pseudo Genes (incomplete)         :: 135 of 209
            Pseudo Genes (internal stop)      :: 41 of 209
            Pseudo Genes (multiple problems)  :: 32 of 209
            Pseudo Genes (short protein)      :: 3 of 209
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058792.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..19857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /strain="STLIN_6"
                     /isolation_source="Stanley WWTPs_6 WWTPs influent"
                     /db_xref="taxon:623"
                     /plasmid="unnamed2"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.2 N 114.2 E"
                     /collection_date="2018-12"
     gene            3..635
                     /locus_tag="HZT08_RS24150"
                     /old_locus_tag="HZT08_23925"
     CDS             3..635
                     /locus_tag="HZT08_RS24150"
                     /old_locus_tag="HZT08_23925"
                     /EC_number="2.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002075262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B-3 chloramphenicol O-acetyltransferase
                     CatB3"
                     /protein_id="WP_000186237.1"
                     /translation="MTNYFDSPFKGKLLSEQVKNPNIKVGRYSYYSGYYHGHSFDDCAR
                     YLFPDRDDVDKLIIGSFCSIGSGASFIMAGNQGHRYDWASSFPFFYMQEEPAFSSALDA
                     FQKAGNTVIGNDVWIGSEAMVMPGIKIGHGAVIGSRSLVTKDVEPYAIVGGNPAKKIKK
                     RFTDEEISLLLEMEWWNWSLEKIKAAMPMLCSSNIVGLHKYWLEFAV"
                     /name="type B-3 chloramphenicol O-acetyltransferase CatB3"
     gene            720..1172
                     /locus_tag="HZT08_RS24155"
                     /old_locus_tag="HZT08_23930"
     CDS             720..1172
                     /locus_tag="HZT08_RS24155"
                     /old_locus_tag="HZT08_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase Arr-3"
                     /protein_id="WP_001749986.1"
                     /translation="MVKDWIPISHDNYKQVQGPFYHGTKANLAIGDLLTTGFISHFEDG
                     RILKHIYFSALMEPAVWGAELAMSLSGLEGRGYIYIVEPTGPFEDDPNLTNKRFPGNPT
                     QSYRTCEPLRIVGVVEDWEGHPVELIRGMLDSLEDLKRRGLHVIED"
                     /name="NAD(+)--rifampin ADP-ribosyltransferase Arr-3"
     gene            1395..1742
                     /locus_tag="HZT08_RS24160"
                     /old_locus_tag="HZT08_23935"
     CDS             1395..1742
                     /locus_tag="HZT08_RS24160"
                     /old_locus_tag="HZT08_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="WP_000679427.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAFY
                     FLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSP
                     SWKSLRRPTPW"
                     /name="quaternary ammonium compound efflux SMR transporter
                     QacE delta 1"
     gene            1736..2575
                     /gene="sul1"
                     /locus_tag="HZT08_RS24165"
                     /old_locus_tag="HZT08_23940"
     ARG             1737..2572
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2703..2906
                     /locus_tag="HZT08_RS24170"
                     /old_locus_tag="HZT08_23945"
     CDS             2703..2906
                     /locus_tag="HZT08_RS24170"
                     /old_locus_tag="HZT08_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000376616.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARID
                     RTFAETFVLRSELLGIASENGK"
                     /name="hypothetical protein"
     gene            complement(3024..3728)
                     /locus_tag="HZT08_RS24175"
                     /old_locus_tag="HZT08_23950"
     CDS             complement(3024..3728)
                     /locus_tag="HZT08_RS24175"
                     /old_locus_tag="HZT08_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042893601.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <3874..4704
                     /locus_tag="HZT08_RS24180"
                     /old_locus_tag="HZT08_23955"
                     /pseudo=""
     CDS             <3874..4704
                     /locus_tag="HZT08_RS24180"
                     /old_locus_tag="HZT08_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /name="IS91-like element ISVsa3 family transposase"
     gene            4735..5619
                     /locus_tag="HZT08_RS24185"
                     /old_locus_tag="HZT08_23960"
     CDS             4735..5619
                     /locus_tag="HZT08_RS24185"
                     /old_locus_tag="HZT08_23960"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="WP_001447541.1"
                     /translation="MLRSQRRGRDPPCWLSEKEPLVGKRRVNFQRCWLPVSRIGLHRRG
                     VERVNCGPKLLDLGEKGVYSSYTCRVPGGDYEGYVDAHVRRLEALRRAGIVERIDADQW
                     RIPDDLVSRAAAHDAGRDSQASVRVLSPVDLNKQIGSDGATWLDRRLIHGETADLAPTG
                     FGQQVREAMDQRREHHIEQGDATRSRDSRVFYRRNLLAILREREVAGVGSDMALSKGLP
                     FRAATDGESVSGKFTGTVHLSSGKFAVVEKSHEFTLVPWRPIIDRQLGREVMGIVQGGS
                     VSWQLGRQRGLER"
                     /name="DUF3363 domain-containing protein"
     gene            5836..7050
                     /gene="floR"
                     /locus_tag="HZT08_RS24190"
                     /old_locus_tag="HZT08_23965"
     ARG             5837..7047
                     /name="floR"
                     /gene="floR"
                     /gene_name="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7078..7383
                     /locus_tag="HZT08_RS24195"
                     /old_locus_tag="HZT08_23970"
     CDS             7078..7383
                     /locus_tag="HZT08_RS24195"
                     /old_locus_tag="HZT08_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_001255015.1"
                     /translation="MRRTNHRNLVNVGILSGRIPLISLVQFIAVAEHLNFRHAAKALGI
                     SQSSVSARVKALEDNLGVLLFERHARGVRLTDAGRHFMERVTAGVDQLDHAVKTAE"
                     /name="LysR family transcriptional regulator"
     gene            7495..8988
                     /locus_tag="HZT08_RS24200"
                     /old_locus_tag="HZT08_23975"
     CDS             7495..8988
                     /locus_tag="HZT08_RS24200"
                     /old_locus_tag="HZT08_23975"
                     /function="catalyse the transposition of transposable
                     elements or transposons"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="WP_015344975.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMAE
                     QGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARRMA
                     ESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKKAGH
                     THQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPELTQL
                     THTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKV
                     FTLQTLPTSGDPFGDGIGKVAGFSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLT
                     RGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALV
                     TPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIED
                     PIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
                     /name="IS91-like element ISVsa3 family transposase"
     gene            complement(<9224..9466)
                     /gene="glmM"
                     /locus_tag="HZT08_RS24205"
                     /old_locus_tag="HZT08_23985"
                     /pseudo=""
     CDS             complement(<9224..9466)
                     /gene="glmM"
                     /locus_tag="HZT08_RS24205"
                     /old_locus_tag="HZT08_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891390.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /name="glmM"
     gene            complement(9553..10368)
                     /gene="sul2"
                     /locus_tag="HZT08_RS24210"
                     /old_locus_tag="HZT08_23990"
     ARG             complement(9557..10368)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            10458..11548
                     /locus_tag="HZT08_RS24215"
                     /old_locus_tag="HZT08_23995"
     CDS             join(10458..10998,10998..11548)
                     /locus_tag="HZT08_RS24215"
                     /old_locus_tag="HZT08_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612069.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISAba1 family transposase"
                     /protein_id="WP_085940648.1"
                     /translation="MTHLNELYLILNKSLKWNKSHLKCFALIMLVIILKQTCNLSSASK
                     ALPIKCLPQSFYRRMQRFFAGQYFDYRQISQLIFNMFSFDQVQLTLDRTNWKWGKRNIN
                     ILMLAIVYRGIAIPILWTLLNKRGNSDTKERIALIQRFIAIFGKDRIVNVFADREFIGE
                     QWFTWLIEQDINFCIRVKKNFIVTNHLGKNHKISDLFRHLKVGQIECRKRRILVGRVKL
                     YISALQLENGELLLVVSPQFNANAIQDYALRWEIETLFSCLKGRGFNLENTRLTDPRRV
                     KKLIAVLAISFCWCYLTGEWQHNQKKAIKIKKHGRLSMSLFRYGLDYVQMAIQRLIGFG
                     KKEEFKEILAILRKQNPDRIRVL"
                     /name="IS4-like element ISAba1 family transposase"
     gene            complement(11745..12230)
                     /locus_tag="HZT08_RS24220"
                     /old_locus_tag="HZT08_24000"
     CDS             complement(11745..12230)
                     /locus_tag="HZT08_RS24220"
                     /old_locus_tag="HZT08_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_006581703.1"
                     /translation="MQIDIKTSSVKPLRNTYAYIEKRFGDKPASRYQEATYDIQEEINF
                     HYKPLWQPEFDLYDKGRTVIQMKDWYVLKDPRQFYYGAYTQTRAKQQEILESNFTLVEK
                     HDLLRNISEEILNKVTKLLLPLYCKQDIFIFYIQWLIFLLIGNTMKNTMLRKGLTIF"
                     /name="hypothetical protein"
     gene            complement(12456..>13979)
                     /locus_tag="HZT08_RS24225"
                     /old_locus_tag="HZT08_24010"
                     /pseudo=""
     CDS             complement(12456..>13979)
                     /locus_tag="HZT08_RS24225"
                     /old_locus_tag="HZT08_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612398.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            14041..14793
                     /locus_tag="HZT08_RS24230"
                     /old_locus_tag="HZT08_24015"
     CDS             14041..14793
                     /locus_tag="HZT08_RS24230"
                     /old_locus_tag="HZT08_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654541.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_000602738.1"
                     /translation="MILNTIAEKLKRQSKDDFKGRHFEAWLIVQAVAWYLRYPLSYRDL
                     EEMFRERGFEVDHSTINRWVLAYAPVIEKRLRQFRRPHCGSVRIDETYVKIRGKWRYLY
                     RAIDKHGNPVDFLLTAKRDLDAAKRFFRKMLKDEPLLSPNRIGTDGANTFPSTIKTSVD
                     DGLLHPDPVHYVTKHLQQGIESDHFRVKKNMPKIGGFQSFNTARRTIAGFEAMLWLRKG
                     FGFSGGWTVNDQNDLLARLFGLQKVNKA"
                     /name="IS6 family transposase"
     gene            complement(<14856..15209)
                     /locus_tag="HZT08_RS24235"
                     /old_locus_tag="HZT08_24020"
                     /pseudo=""
     CDS             complement(<14856..15209)
                     /locus_tag="HZT08_RS24235"
                     /old_locus_tag="HZT08_24020"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /name="AAC(3) family N-acetyltransferase"
     gene            complement(15215..16240)
                     /gene="aph(4)-Ia"
                     /locus_tag="HZT08_RS24240"
                     /old_locus_tag="HZT08_24025"
     ARG             complement(15219..16240)
                     /name="aph(4)-Ia"
                     /gene="aph(4)-Ia"
                     /gene_name="aminoglycoside O-phosphotransferase APH(4)-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(16469..17245)
                     /gene="aac(3)-IVa"
                     /locus_tag="HZT08_RS24245"
                     /old_locus_tag="HZT08_24030"
     ARG             complement(16473..17245)
                     /name="aac(3)-IVa"
                     /gene="aac(3)-IVa"
                     /gene_name="aminoglycoside N-acetyltransferase AAC(3)-IVa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            17359..18063
                     /locus_tag="HZT08_RS24250"
                     /old_locus_tag="HZT08_24035"
     CDS             17359..18063
                     /locus_tag="HZT08_RS24250"
                     /old_locus_tag="HZT08_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            18207..18761
                     /locus_tag="HZT08_RS24255"
                     /old_locus_tag="HZT08_24040"
     CDS             18207..18761
                     /locus_tag="HZT08_RS24255"
                     /old_locus_tag="HZT08_24040"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoroquinolone-acetylating aminoglycoside
                     6'-N-acetyltransferase AAC(6')-Ib-cr5"
                     /protein_id="WP_063840321.1"
                     /translation="MTNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLAD
                     VQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGRWEEETDPGVRGIDQLLA
                     NASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFERQGTVTTP
                     YGPAVYMVQTRQAFERTRSDA"
                     /name="fluoroquinolone-acetylating aminoglycoside
                     6'-N-acetyltransferase AAC(6')-Ib-cr5"
     gene            18892..19722
                     /locus_tag="HZT08_RS24260"
                     /old_locus_tag="HZT08_24045"
     CDS             18892..19722
                     /locus_tag="HZT08_RS24260"
                     /old_locus_tag="HZT08_24045"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="WP_001334766.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCFL
                     LYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGMEI
                     WNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLTEAW
                     LESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFTA
                     NRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
                     /name="oxacillin-hydrolyzing class D beta-lactamase OXA-1"
     ARG             1396..1739
                     /name="qacEdelta1"
                     /gene="qacEdelta1"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             721..1169
                     /name="arr-3"
                     /gene="arr-3"
                     /gene_name="NAD(+)--rifampin ADP-ribosyltransferase Arr-3"
                     /tool="amrfinderplus"
                     /drug_class="RIFAMYCIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             18208..18758
                     /name="aac(6')-Ib-cr5"
                     /gene="aac(6')-Ib-cr5"
                     /gene_name="fluoroquinolone-acetylating aminoglycoside
                     6'-N-acetyltransferase AAC(6')-Ib-cr5"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE/QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             18893..19719
                     /name="blaOXA-1"
                     /gene="blaOXA-1"
                     /gene_name="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4..632
                     /name="catB3"
                     /gene="catB3"
                     /gene_name="type B-3 chloramphenicol O-acetyltransferase
                     CatB3"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             18163..18761
                     /name="AAC(6')-Ib-cr6"
                     /gene="AAC(6')-Ib-cr6"
                     /gene_name="AAC(6'); AAC(6')-Ib-cr"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic; aminoglycoside
                     antibiotic"
                     /identity="98.99"
                     /coverage="100.0"
ORIGIN
        1 ttatgaccaa ctactttgat agccccttca aaggcaagct gctttctgag caagtgaaga
       61 accccaatat caaagttggg cggtacagct attactctgg ctactatcat gggcactcat
      121 tcgatgactg cgcacggtat ctgtttccgg accgtgatga cgttgataag ttgatcatcg
      181 gtagtttctg ctctatcggg agtggggctt cctttatcat ggctggcaat caggggcatc
      241 ggtacgactg ggcatcatct ttcccgttct tttatatgca ggaagaacct gcattctcaa
      301 gcgcactcga tgccttccaa aaagcaggta atactgtcat tggcaatgac gtttggatcg
      361 gctctgaggc aatggtcatg cccggaatca agatcgggca cggtgcggtg ataggcagcc
      421 gctcgttggt gacaaaagat gtggagcctt acgctatcgt tggcggcaat cccgctaaga
      481 agattaagaa acgcttcacc gatgaggaaa tttcattgct tctggagatg gagtggtgga
      541 attggtcact ggagaagatc aaagcggcaa tgcccatgct gtgctcgtct aatattgttg
      601 gcctgcacaa gtattggctc gagtttgccg tctaacaatt caatcaagcc gatgccgctt
      661 cgcggcacgg cttatttcag gcgttatgca gccaaatccc aacaattaag ggtcttaaaa
      721 tggtaaaaga ttggattccc atctctcatg ataattacaa gcaggtgcaa ggaccgttct
      781 atcatggaac caaagccaat ttggcgattg gtgacttgct aaccacaggg ttcatctctc
      841 atttcgagga cggtcgtatt cttaagcaca tctacttttc agccttgatg gagccagcag
      901 tttggggagc tgaacttgct atgtcactgt ctggcctcga gggtcgcggc tacatataca
      961 tagttgagcc aacaggaccg ttcgaagacg atccgaatct tacgaacaaa agatttcccg
     1021 gtaatccaac acagtcctat agaacctgcg aacccttgag aattgttggc gttgttgaag
     1081 actgggaggg gcatcctgtt gaattaataa ggggaatgtt ggattcgttg gaggacttaa
     1141 agcgccgtgg tttacacgtc attgaagact agtcctttgc ataacaaagc catcaaaccg
     1201 gacgccagag attccgcgcc tgttgcgcat ggcttcgcca ttttatgcgc aataggcgcg
     1261 ccaccctgtc gccgtttatg gcggcgttag atgcactaag cacataattg ctcacagcca
     1321 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa
     1381 ttgggagata tatcatgaaa ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa
     1441 tcgcaacatc cgcattaaaa tctagcgagg gctttactaa gcttgcccct tccgccgttg
     1501 tcataatcgg ttatggcatc gcattttatt ttctttctct ggttctgaaa tccatccctg
     1561 tcggtgttgc ttatgcagtc tggtcgggac tcggcgtcgt cataattaca gccattgcct
     1621 ggttgcttca tgggcaaaag cttgatgcgt ggggctttgt aggtatgggg ctcataattg
     1681 ctgccttttt gctcgcccga tccccatcgt ggaagtcgct gcggaggccg acgccatggt
     1741 gacggtgttc ggcattctga atctcaccga ggactccttc ttcgatgaga gccggcggct
     1801 agaccccgcc ggcgctgtca ccgcggcgat cgaaatgctg cgagtcggat cagacgtcgt
     1861 ggatgtcgga ccggccgcca gccatccgga cgcgaggcct gtatcgccgg ccgatgagat
     1921 cagacgtatt gcgccgctct tagacgccct gtccgatcag atgcaccgtg tttcaatcga
     1981 cagcttccaa ccggaaaccc agcgctatgc gctcaagcgc ggcgtgggct acctgaacga
     2041 tatccaagga tttcctgacc ctgcgctcta tcccgatatt gctgaggcgg actgcaggct
     2101 ggtggttatg cactcagcgc agcgggatgg catcgccacc cgcaccggtc accttcgacc
     2161 cgaagacgcg ctcgacgaga ttgtgcggtt cttcgaggcg cgggtttccg ccttgcgacg
     2221 gagcggggtc gctgccgacc ggctcatcct cgatccgggg atgggatttt tcttgagccc
     2281 cgcaccggaa acatcgctgc acgtgctgtc gaaccttcaa aagctgaagt cggcgttggg
     2341 gcttccgcta ttggtctcgg tgtcgcggaa atccttcttg ggcgccaccg ttggccttcc
     2401 tgtaaaggat ctgggtccag cgagccttgc ggcggaactt cacgcgatcg gcaatggcgc
     2461 tgactacgtc cgcacccacg cgcctggaga tctgcgaagc gcaatcacct tctcggaaac
     2521 cctcgcgaaa tttcgcagtc gcgacgccag agaccgaggg ttagatcatg cctagcattc
     2581 accttccggc cgcccgctag cggaccctgg tcaggttccg cgaaggtggg cgcagacatg
     2641 ctgggctcgt caggatcaaa ctgcactatg aggcggcggt tcataccgcg ccaggggagc
     2701 gaatggacag cgaggagcct ccgaacgttc gggtcgcctg ctcgggtgat atcgacgagg
     2761 ttgtgcggct gatgcacgac gctgcggcgt ggatgtccgc caagggaacg cccgcctggg
     2821 acgtcgcgcg gatcgaccgg acattcgcgg agaccttcgt cctgagatcc gagctcctag
     2881 ggatcgcctc agaaaacgga aaataaagca cgctaagccg taagtaagcg tgctcctgtg
     2941 aaagccacag ctaaaactgc gtagtacaca tggcactgtt gcaaagttag cgatgaggca
     3001 gccttttgtc ttattcaaag gccttacatt tcaaaaactc tgcttaccag gcgcatttcg
     3061 cccaggggat caccataata aaatgctgag gcctggcctt tgcgtagtgc acgcatcacc
     3121 tcaatacctt tgatggtggc gtaagccgtc ttcatggatt taaatcccag cgtggcgccg
     3181 attatccgtt tcagtttgcc atgatcgcat tcaatcacgt tgttccggta cttaatctgt
     3241 cggtgttcaa cgtcagacgg gcaccggcct tcgcgtttga gcagagcaag cgcgcgacca
     3301 taggcgggcg ctttatccgt gttgatgaat cgcgggatct gccacttctt cacgttgttg
     3361 aggattttac ccagaaaccg gtatgcagct ttgctgttac gacgggagga gagataaaaa
     3421 tcgacagtgc ggccccggct gtcgacggcc cggtacagat acgcccagcg gccattgacc
     3481 ttcacgtagg tttcatccat gtgccacggg caaagatcgg aagggttacg ccagtaccag
     3541 cgcagccgtt tttccatttc aggcgcataa cgctgaaccc agcggtaaat cgtggagtga
     3601 tcgacattca ctccgcgttc agccagcatc tcctgcagct cacggtaact gatgccgtat
     3661 ttgcagtacc agcgtacggc ccacagaatg atgtcacgct gaaaatgccg gcctttgaat
     3721 gggttcatgt gcagctccat cagcaaaagg ggatgataag tttatcacca ccgactattt
     3781 gcaacagtgc cgaccatgga aaggattcat atgctgcacc tttagctaaa acagtcttca
     3841 gcttaccatt cgtggttatt tgcaacagtg ccgctgacgc acaccatcgc ccaccgggtg
     3901 ggtcgctatc tggaacggca aggcctgctg gaacgggatg tcgaaaacag ctatctggcc
     3961 tcggatgcgg tggatgacga cccgatgaca cccctgctgg ggcactcgat cacttaccgt
     4021 atcgctgtcg gttcacaggc ggggcgaaag gtgttcactt tgcaaactct gccgaccagt
     4081 ggtgatccgt tcggtgacgg gattggcaag gtagccgggt tcagcctgca cgccggcgtg
     4141 gcggccaggg ccgatgaacg caagaagctc gaacggctgt gccggtacat cagccgcccg
     4201 gcggtatccg agaagcggct gtcgttaaca cgaggcggca acgtgcgcta ccagctcaag
     4261 acgccgtacc gggacggcac cacgcacgtc attttcgaac cattggattt cattgcaagg
     4321 ctggccgccc tggtaccgaa gcccagagtc aacctaaccc gcttccacgg ggtgttcgca
     4381 cccaacagtc ggcaccgggc gttggtcacg ccggcaaaac ggggcagggg caacaaggtc
     4441 agggtggctg atgaaccggc aacaccagca caacggcgag cgtcgatgac atgggcgcaa
     4501 cggctcaagc gtgttttcaa tatcgacatc gagacctgca gcggctgcgg cggcgccatg
     4561 aaagtcatcg cctgcattga agaccctata gtgatcaagc agatccttga tcacctgaag
     4621 cacaaagccg aaaccagcgg gaccagggcg ttacccgaaa gccgggcgcc accggctgag
     4681 ctgctcctgg gtctgtttga ctgacgagcc tgaaggccaa cgataccaat caaaatgctg
     4741 cgttcacagc gccgcggcag ggatccgccg tgctggttgt cggaaaagga gccgctagtg
     4801 ggaaagagga gggtaaattt tcagcgttgc tggctccccg tcagccggat tgggttgcat
     4861 cgcaggggtg tcgaaagagt caactgcggt ccaaagctgt tggacttggg tgaaaagggc
     4921 gtttattctt cctatacgtg ccgcgttcca ggcggcgact atgagggcta tgtcgatgcc
     4981 catgtgcgcc ggctggaggc gctacgccgg gccggtatcg tcgagcggat cgacgccgac
     5041 caatggcgca tccccgatga tctggtcagc cgtgccgccg cccatgacgc cggccgagac
     5101 agtcaggcca gcgttcgcgt cctttccccg gtcgatctga acaaacagat cggatcggac
     5161 ggcgcgacct ggctggaccg gcggctgatc cacggcgaga cggccgacct tgcgccaacc
     5221 ggcttcgggc aacaagtccg cgaagccatg gaccagcgcc gcgagcacca tatcgaacag
     5281 ggcgacgcca cccgcagccg ggacagccgc gtcttctacc ggcgcaacct tctcgccatc
     5341 ctgcgggagc gcgaggtagc cggcgtcgga tcggatatgg ctttgagtaa gggcctgccg
     5401 ttccgcgccg ccacggacgg cgagagcgtc agcggcaagt ttaccggaac cgtgcatcta
     5461 tcgagcggca agttcgccgt ggtcgagaaa tcccatgagt tcacccttgt cccgtggcgg
     5521 ccgatcatcg accgccaact cggccgcgag gttatgggca tcgtgcaggg cgggtcggtg
     5581 tcgtggcagt tagggcggca gagggggctg gaacgctgag tgcgcccatg ccgcattgcg
     5641 aagcaaaaga taatcggata aaatgtagca attcatattc gtaagcgtgg agtaatcaga
     5701 tgggaaattc caagtcagca gacaagtaag ccgcaacaac cagtattgtt gttgcggcgc
     5761 tctgtaaggc tagtctcatc tgattgctga cgagcagacg tcgcccggta ttccttaatc
     5821 gagggttgat tcgtcatgac caccacacgc cccgcgtggg cctatacgct gccggcagca
     5881 ctgctgctga tggctccttt cgacatcctc gcttcactgg cgatggatat ttatctccct
     5941 gtcgttccag cgatgcccgg catcctgaac acgacgcccg ctatgatcca actcacgttg
     6001 agcctctata tggtgatgct cggcgtgggc caggtgattt ttggtccgct ctcagacaga
     6061 atcgggcgac ggccaattct acttgcgggc gcaacggctt tcgtcattgc gtctctggga
     6121 gcagcttggt cttcaactgc accggccttt gtcgctttcc gtctacttca agcagtgggc
     6181 gcgtcggcca tgctggtggc gacgttcgcg acggttcgcg acgtttatgc caaccgtcct
     6241 gagggtgtcg tcatctacgg ccttttcagt tcgatgctgg cgttcgtgcc tgcgctcggc
     6301 cctatcgccg gagtattgat cggcgagttc ttgggatggc aggcgatatt cattactttg
     6361 gctatactgg cgatgctcgc actcctaaat gcgggtttca ggtggcacga aacccgccct
     6421 ctggatcaag tcaagacgcg ccgatctgtc ttgccgatct tcgcgagtcc ggctttttgg
     6481 gtttacactg tcggctttag cgccggtatg ggcacctact tcgtcttctt ctcgacggct
     6541 ccccgtgtgc tcataggcca agcggaatat tccgagatcg gattcagctt tgccttcgcc
     6601 actgtcgcgc ttgtaatgat cgtgacaacc cgtttcgcga agtcctttgt cgccagatgg
     6661 ggcatcgcag gatgcgtggc gcgtgggatg gcgttgcttg tttgcggagc ggtcctgttg
     6721 gggatcggcg aactttacgg ctcgccgtca ttcctcacct tcatcctacc gatgtgggtt
     6781 gtcgcggtcg gtattgtctt cacggtgtcc gttaccgcga acggcgcttt ggcagagttc
     6841 gacgacatcg cgggatcagc ggtcgcgttc tacttctgcg ttcaaagcct gatagtcagc
     6901 attgtcggga cattggcggt ggcactttta aacggtgaca cagcgtggcc cgtgatctgt
     6961 tacgccacgg cgatggcggt actggtttcg ttggggctgg tgctccttcg gctccgtggg
     7021 gctgccaccg agaagtcgcc agtcgtctaa ccgacgactg gtagcaggcc cgctccgatg
     7081 cggcgcacta accatcgaaa cctcgtgaat gtcggtatcc tgtctggcag gataccgctc
     7141 atttcccttg ttcagttcat cgccgtcgcc gagcatctga attttcggca tgcggccaag
     7201 gcacttggta tcagccagtc gagcgtcagc gcgcgtgtga aagcgctgga ggataacctt
     7261 ggtgtcctgc tatttgagcg ccatgcgcgg ggcgttcggc taacagacgc aggcaggcac
     7321 ttcatggagc gtgtcacggc gggtgtcgat caactcgatc acgcagtgaa gaccgcggag
     7381 tgacgggcac tggctggcaa tgtctagcaa cggcaggcat ttcggctgag ggtaaaagaa
     7441 ctttccgcta agcgatagac tgtatgtaaa cacagtattg caaggacgcg gaacatgcct
     7501 catgtggcgg ccaggacggc cagccgggat cgggatactg gtcgttacca gagccaccga
     7561 cccgagcaaa cccttctcta tcagatcgtt gacgagtatt acccggcatt cgctgcgctt
     7621 atggcagagc agggaaagga attgccgggc tatgtgcaac gggaatttga agaatttctc
     7681 caatgcgggc ggctggagca tggctttcta cgggttcgct gcgagtcttg ccacgccgag
     7741 cacctggtcg ctttcagctg taagcgtcgc ggtttctgcc cgagctgtgg ggcgcggcgg
     7801 atggccgaaa gtgccgcctt gctggttgat gaagtactgc ctgaacaacc catgcgtcag
     7861 tgggtgttga gcttcccgtt tcagctgcgt ttcctgtttg ccagccggcc cgagatcatg
     7921 gggtgggtgc tgggcatcgt ttaccgcgtc attgccacgc acctggtcaa gaaagcgggc
     7981 catacccacc aagtggccaa gacgggcgcg gtcaccctga tccagcgttt tggatcggcg
     8041 ctcaatctga atgttcactt ccacatgctg tttctcgacg gtgtgtatgt cgagcaatcc
     8101 cacggctcag cgcgtttccg ctgggtcaag gcgccgacca gcccagagct cacccagctg
     8161 acgcacacca tcgcccaccg ggtgggtcgc tatctggaac ggcaaggcct gctggaacgg
     8221 gatgtcgaaa acagctatct ggcctcggat gcggtggatg acgacccgat gacacccctg
     8281 ctggggcact cgatcactta ccgtatcgct gtcggttcac aggcggggcg aaaggtgttc
     8341 actttgcaaa ctctgccgac cagtggtgat ccgttcggtg acgggattgg caaggtagcc
     8401 gggttcagcc tgcacgccgg cgtggcggcc agggccgatg aacgcaagaa gctcgaacgg
     8461 ctgtgccggt acatcagccg cccggcggta tccgagaagc ggctgtcgtt aacacgaggc
     8521 ggcaacgtgc gctaccagct caagacgccg taccgggacg gcaccacgca cgtcattttc
     8581 gaaccattgg atttcattgc aaggctggcc gccctggtac cgaagcccag agtcaaccta
     8641 acccgcttcc acggggtgtt cgcacccaac agtcggcacc gggcgttggt cacgccggca
     8701 aaacggggca ggggcaacaa ggtcagggtg gctgatgaac cggcaacacc agcacaacgg
     8761 cgagcgtcga tgacatgggc gcaacggctc aagcgtgttt tcaatatcga catcgagacc
     8821 tgcagcggct gcggcggcgc catgaaagtc atcgcctgca ttgaagaccc tatagtgatc
     8881 aagcagatcc ttgatcacct gaagcacaaa gccgaaacca gcgggaccag ggcgttaccc
     8941 gaaagccggg cgccaccggc tgagctgctc ctgggtctgt ttgactgacg agcctgaagg
     9001 ccaacgatac caatcaaaat gctgcgttca cagcgccgcg gcagggatcc gccgtgctgg
     9061 ttgtcggaaa aggagccgct agtgggaaag aggagggtaa attttcagcg ttgctggctc
     9121 cccgtcagcc ggattgggtt gcatcgcagg ggtgtcgaaa gagtcaactg cggtccaaag
     9181 ctgttggact tgggtgaaaa gggcgtttat tcttcctata cgttgtcggc agcgggccaa
     9241 aaaggaatac gtccatgccc atcgaggtga aaccggctgt gagcgcgggt tcaagcatat
     9301 agcccgacag gcgcgtatcc ttgccgatca cgacacgatg gcggtggtca ccgcgacgaa
     9361 agacacggcc agccgccatg ccgacgcgca aggcggtttc cgccgtcatc gcgccttcgt
     9421 tggctttgcc acgaataccg tctgtgccga aatatttgcg caccataagg tcgattatcc
     9481 tgtcgtcggg tcgccctcaa aggggacatg cctgctgaac cgcgaatata gagaaatatc
     9541 ccgaatgtgc agttaacgaa ttcttgcggt ttctttcagc gccgccaata ccgccagccc
     9601 gtcgcgcaag gggcgcggct cgtgtgtgcg gatgaagtca gctccacctg cggcggcggc
     9661 aagctctgca gcgagtgtcg cggccccgac atcccccgga ccacggcctg tgagcgcgcg
     9721 cagaaaggat ttgcgcgaaa cagacagaag caccggcaaa tcgaagcgca gccgcaattc
     9781 atcgaaccgc gccagcaccg agagcgaggt ttcgggagca gcccccagaa aaaaccccat
     9841 gccgggatca aggacaaggc ggttgcgttt gataccggca cccgtcagcg ccgcgatgcg
     9901 cgcgtcaaag aacgccgcaa tgtgatccat gatgtcgcca gcgggtgcct cgcgccgatc
     9961 tgcctgcccg tcttgcaccg aatgcataac gacgagtttg gcagatgatt tcgccaattg
    10021 cggatagaac gcagcgtctg gaaaaccgcg aatatcattg agataggcca caccacgcga
    10081 caaggcatag gcttgcgtcg cgggttgata actgtcgagc gagacgggaa tgccatctgc
    10141 cttgagcgcg tccagcaccg gcgcgatacg cgcgatttct gtgtcggacg aaacaggcgc
    10201 ggcgtcggga ttgctggatg ccggaccgag gtcgatcaca tctgccccct cggccatcag
    10261 cttacgcgcc tgcgcaatgg ctgcgtctgg cgccagatac cggcctccat cggagaaact
    10321 gtccgaggtt atgttgacga tgccgaaaat gatgagcgat ttattcatgg gggcttctat
    10381 aataatctct gtacacgaca aaaatagata actcattgaa ataatgtcac aataattgtt
    10441 ttctaacgac gaatactatg acacatctca atgagttata tcttatctta aacaaatctc
    10501 taaaatggaa caagtcacat ttaaagtgct ttgcgctcat catgcttgtg attattttaa
    10561 agcaaacatg taatctttct tctgcatcta aagccttgcc catcaagtgt ttaccacaat
    10621 cattttatcg acgtatgcag cgcttctttg caggtcagta ttttgattat cgtcaaattt
    10681 ctcagttgat tttcaatatg ttttcattcg accaagtgca actgacttta gatagaacca
    10741 attggaaatg gggaaaacga aatattaata tcctgatgct cgcaatcgtt tatcgtggaa
    10801 tagcgatacc tatcctttgg acattgctta ataaacgtgg aaattcagat acgaaagagc
    10861 gtattgcttt gattcaacgc tttatagcca tttttggtaa agaccgtatt gtgaatgtgt
    10921 tcgcagacag agagtttatc ggtgagcagt ggtttacatg gttaattgaa caagacatca
    10981 acttctgcat tcgtgttaaa aaaacttcat tgtcaccaat catttaggaa agaatcataa
    11041 aattagtgat ttatttcgcc atcttaaagt tggtcaaatt gaatgtcgta aacgacggat
    11101 tttggttggt cgggtgaaac tatatataag tgcactacag ttagaaaatg gagagctttt
    11161 actcgtcgtt tctcctcagt ttaatgccaa tgctattcag gattatgcat tacgctggga
    11221 aattgaaacc ttattcagtt gtctcaaagg acgcgggttt aatcttgaaa atacgcgctt
    11281 gacagaccct agacgagtga aaaaattgat tgcggtgtta gctataagct tctgttggtg
    11341 ttacttaacg ggtgaatggc aacataatca aaaaaaagcg ataaaaataa agaagcatgg
    11401 acgactctca atgagtttat ttcgctatgg tttagactat gttcaaatgg cgattcagcg
    11461 tttaattggt tttgggaaaa aagaagagtt taaggaaatt ttggcaattt taagaaagca
    11521 gaatcctgat aggataaggg ttctgtgaaa tttgtcgtgt acagagtata ataataataa
    11581 tcgagcatga gtctcatacg gatgctcggg tcgaaaggga atccccaggc gagtaacctg
    11641 tttgcggtga tccattagct gcaggagcag aagagcatac atctggaagc aaagccagga
    11701 aagcggccta tggagctgtg cggcagcgct cagtaggcaa tttttcaaaa tattgttaag
    11761 ccttttctga gcatggtatt tttcatggta ttaccaatta gcaggaaaat aagccattga
    11821 atataaaaga taaaaatgtc ttgtttacaa taaagtggga gtagtaattt cgttactttg
    11881 tttagaattt cttcagaaat atttcttaat aaatcgtgtt tttcaacaag cgtaaaatta
    11941 ctttctaaaa tctcttgttg tttagctcga gtttgggtat aagccccata ataaaattga
    12001 cgaggatctt taagcacgta ccaatctttc atttggatta cagtacggcc cttatcgtaa
    12061 agatcaaatt caggctgcca taaaggtttg taatgaaaat taatttcttc ctgaatatcg
    12121 taggtcgctt cttgataacg tgaagctggt ttatcaccaa atcttttttc aatataagca
    12181 taagtatttc ttaaaggctt aactgaagat gttttaatat caatttgcat ataaatcttc
    12241 ctgatttttt aataaagact agagttttta ttctctaata ccggcctgtc caaatcgcca
    12301 cttcatgcgg tcatattcaa tctttgcaaa atcatccgta cttaggtgct gtacttgatg
    12361 gtgtgcgcag aattcttcaa aagccttgac tggcataatc atttctacag ctaactctgg
    12421 atggggtcgt ttgcgggagg gggcggaatc ctacgctaag gctttggcca gcgatattct
    12481 ccggtgagat tgatgtgttc ccatccgagc ggcgaaacat gggccaagag atcgggcgat
    12541 agcagctttc catcgcgttt ctggtttgca acgacctcgc cgagcttcat ggtgttccag
    12601 aagatgatga tggcggcgag cagattcatg ccggcgatgc ggtaatgctg gccttcggcg
    12661 gaacggtcgc ggatttcacc gcggcggtgg aagctgattg cccgcttcag cgcatgatga
    12721 gcttcgcctt tgttgagccc gatctgggca cgccgttgga gttcggcatc cagaatccag
    12781 tcgatcatga acagggtgcg ctcgacgcga ccgacttccc gcagggctgt cgcgagctcg
    12841 ttctgccgcg gataggaggc gagtttccgc agaatctggc ttggcgcgac ggtcccggca
    12901 gcaatggtgg cggcgatgcg caggatgtcg ggccaattgc gctcgatcat ggcttggttg
    12961 acctttccgc cgatcaacgc tcgcaggtgc gccggggcgg ccgacggatt gaacgcgtag
    13021 agccgtttgg atggcaggtc gcggatgcgc ggagcgaacc ggtagccgag aatggcacat
    13081 gcggcaaaga cgtgatcggt gaagccgccc gtgtcggtga actgctcgcg gatatggcgt
    13141 ccagcatcgt tcatcagcag gccatcgagg atgtaaggcg cttcgcttgc cgttgcagga
    13201 atcacctggg ttgcgaacgg cgcatattgg tcggagacgt ggctataggc tttcaggccc
    13261 ggggtattgc catatttcgc gttgaccagg ttcatggcct caccttgctc tgtagcgacg
    13321 aagaactgtc cgtcgctcga agccgacgtg cccatgcccc agaaccgggc catgggtaac
    13381 gctgcctgtg cctcgaccac catggccagc gcccggtcat aggcttcgcc ctcgacatgc
    13441 caccgtccaa tgcggatcaa ttcccagaag gtgtgggtgt ttgtcgcatc cgccattttg
    13501 cgcaagccga ggttgatccc ttccgccaag ataacgttca ttagcccgat ccggtcagcg
    13561 cagggtgctc ctgtgcgcag atgggtgaac gcttcggtga agccggtcgc cgcatccacc
    13621 tccagcagga gatcggtgat gcgcgtgggc gggatctgct tgtagagatc gagcaccaga
    13681 tcttcggcgc ctgtcggcgc ggcggcttcg agtttctcga tatgcagaac gccgttttca
    13741 atcgacccgc ccgggatcgt gcctgcgcga gcggcacggc caagctcgcg caaccgcatg
    13801 tcgaggcgag cttgccggtc tgccagccat tcctccggcc gcaatggcac agcgagacga
    13861 ccgccttccg cgatggattg tgccggaacg agtgcgtgtt tcagatcgcc atagcgccgg
    13921 gacctagtaa gccagacatc tccggagcgg aacgcatggc cttgtcgcaa attcttcagg
    13981 ttaactcgat gttgaccatg gggagagaag ttgccgctcc tgatattgcg gagcgagccg
    14041 atgattttga ataccattgc cgaaaagctg aagcgccagt cgaaggatga tttcaagggc
    14101 aggcatttcg aggcctggct gattgtacag gcggttgcct ggtacttgcg ctacccgctc
    14161 agctatcgtg acttggaaga gatgtttcgc gagcggggct tcgaggtcga tcatagcacg
    14221 atcaaccgct gggtattggc ttatgcacct gtcatcgaga aacggctgcg gcagtttcgt
    14281 cgaccccatt gcggctcggt ccggattgat gagacctatg tcaagatccg cggcaaatgg
    14341 cgctatctgt accgagccat cgataagcat ggcaatccgg tggatttcct gctgaccgct
    14401 aagcgcgatc tcgacgctgc caagcggttc ttccgaaaga tgcttaaaga tgaacccttg
    14461 ttgtcgccga acaggattgg gacggacggg gccaacacct tcccgtcaac gatcaaaacg
    14521 tcggttgatg atgggcttct ccatcccgat cccgtgcatt atgtgaccaa acatctccag
    14581 caggggattg agagcgacca tttccgggtg aagaagaaca tgccgaagat cggcggtttc
    14641 caatccttta acacggcgcg gcgaaccatc gcgggtttcg aggcgatgct gtggctgcgg
    14701 aagggctttg gcttttcagg cggctggacc gtcaatgacc agaatgattt gcttgcgcgc
    14761 ctcttcggac tgcaaaaggt taacaaagca tgaaaatacc ggccttggct gggtgatagc
    14821 tgcctccaaa agtgtttgcg acacgcccgg aacaattcag ccactgcacc catgttccga
    14881 gttggtgtgg tttgcggatc gtatgccggc atctcgttgc gcaagatctg cacccaatcg
    14941 gcaggcacgg gcggcgatct ccaatctgcg ggatcagtca gatcacccga gtgcgtgggc
    15001 atgacaatcg tgccctgggg accaacacaa tccagaaggg cctgaatcac tgcgaccggc
    15061 cctcccgcga cccagccgag cgagcttagc gaactgtgga cgagaactgt gccaccaagc
    15121 gtaaggccgt tctctcgcat ttgccttgct aggctcgcgc gagttgctgg ctgaggcgtt
    15181 ctcgaaatca gctcttgttc ggtcggcatc tactctattc ctttgccctc ggacgagtgc
    15241 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
    15301 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
    15361 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
    15421 gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg
    15481 gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga
    15541 agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
    15601 ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga
    15661 ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga
    15721 cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
    15781 tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
    15841 cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg
    15901 gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac
    15961 ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa
    16021 tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
    16081 cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
    16141 attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
    16201 acttctcgac agacgtcgcg gtgagttcag gctttttcat atctcattgc ccccggacga
    16261 gcgtctgctc cgccattcgc cgtccgccgt gccaatcgga tcagccgtcc aaatgcggga
    16321 ttttcgttag tcggaggcca aacggcattg agcgtcagca tatcatcagc gagctgaaga
    16381 aagacaatcc ccgatccgct ccacgtgttg ccccagcaat cagcgcgacc ttgcccctcc
    16441 aacgtcatct cgttctccgc tcatgagctc agccaatcga ctggcgagcg gcatcgcatt
    16501 cttcgcatcc cgcctctggc ggatgcagga agatcaacgg atctcggccc agttgaccca
    16561 gggctgtcgc cacaatgtcg cgggagcgaa tcaaccgagc aaaggcatga ccgactggac
    16621 cttccttctg aaggctcttc tccttgagcc acctgtccgc caaggcaaag cgctcacagc
    16681 agtggtcatt ctcgagataa tcgacgcgta ccaacttgcc atcctgaaga atggtgcagt
    16741 gtctcggcac cccataggga acctttgcca tcaactcggc aagatgcagc gtcgtgttgg
    16801 catcgtgtcc cacgccgagg agaagtacct gcccatcgag ttcatggaca cgggcgaccg
    16861 ggcttgcagg cgagtgaggt ggcaggggca atggatcaga gatgatctgc tctgcctgtg
    16921 gccccgctgc cgcaaaggca aatggatggg cgctgcgctt tacatttggc aggcgccaga
    16981 atgtgtcaga gacaactcca aggtccggtg taacgggcga cgtggcagga tcgaacggct
    17041 cgtcgtccag acctgaccac gagggcatga cgagcgtccc tcccggaccc agcgcagcac
    17101 gcagggcctc gatcagtcca agtggcccat cttcgagggg ccggacgcta cggaaggagc
    17161 tgtggaccag cagcacaccg ccgggggtaa ccccaaggtt gagaagctga ccgatgagct
    17221 cggcttttcg ccattcgtat tgcacgacat tgcactccac cgctgatgac atcagtcgat
    17281 catagcacga tcaacggcac tgttgcaaat agtcggtggt gataaactta tcatcccctt
    17341 ttgctgatgg agctgcacat gaacccattc aaaggccggc attttcagcg tgacatcatt
    17401 ctgtgggccg tacgctggta ctgcaaatac ggcatcagtt accgtgagct gcaggagatg
    17461 ctggctgaac gcggagtgaa tgtcgatcac tccacgattt accgctgggt tcagcgttat
    17521 gcgcctgaaa tggaaaaacg gctgcgctgg tactggcgta acccttccga tctttgcccg
    17581 tggcacatgg atgaaaccta cgtgaaggtc aatggccgct gggcgtatct gtaccgggcc
    17641 gtcgacagcc ggggccgcac tgtcgatttt tatctctcct cccgtcgtaa cagcaaagct
    17701 gcataccggt ttctgggtaa aatcctcaac aacgtgaaga agtggcagat cccgcgattc
    17761 atcaacacgg ataaagcgcc cgcctatggt cgcgcgcttg ctctgctcaa acgcgaaggc
    17821 cggtgcccgt ctgacgttga acaccgacag attaagtacc ggaacaacgt gattgaatgc
    17881 gatcatggca aactgaaacg gataatcggc gccacgctgg gatttaaatc catgaagacg
    17941 gcttacgcca ccatcaaagg tattgaggtg atgcgtgcac tacgcaaagg ccaggcctca
    18001 gcattttatt atggtgatcc cctgggcgaa atgcgcctgg taagcagagt ttttgaaatg
    18061 taaggccttt gaataagaca aaaggctgcc tcatcgctaa ctttgcaaca gtgcctttaa
    18121 gcgtgcataa taagccctac acaaattggg agttagacat catgagcaac gcaaaaacaa
    18181 agttaggcat cacaaagtac agcatcgtga ccaacagcaa cgattccgtc acactgcgcc
    18241 tcatgactga gcatgacctt gcgatgctct atgagtggct aaatcgatct catatcgtcg
    18301 agtggtgggg cggagaagaa gcacgcccga cacttgctga cgtacaggaa cagtacttgc
    18361 caagcgtttt agcgcaagag tccgtcactc catacattgc aatgctgaat ggagagccga
    18421 ttgggtatgc ccagtcgtac gttgctcttg gaagcgggga cggacggtgg gaagaagaaa
    18481 ccgatccagg agtacgcgga atagaccagt tactggcgaa tgcatcacaa ctgggcaaag
    18541 gcttgggaac caagctggtt cgagctctgg ttgagttgct gttcaatgat cccgaggtca
    18601 ccaagatcca aacggacccg tcgccgagca acttgcgagc gatccgatgc tacgagaaag
    18661 cggggtttga gaggcaaggt accgtaacca ccccatatgg tccagccgtg tacatggttc
    18721 aaacacgcca ggcattcgag cgaacacgca gtgatgccta acccttccat cgagggggac
    18781 gtccaagggc tggcgccctt ggccgcccct catgtcaaac gttgggcgaa cccggagcct
    18841 cattaattgt tagccgttaa aattaagccc tttaccaaac caatacttat tatgaaaaac
    18901 acaatacata tcaacttcgc tattttttta ataattgcaa atattatcta cagcagcgcc
    18961 agtgcatcaa cagatatctc tactgttgca tctccattat ttgaaggaac tgaaggttgt
    19021 tttttacttt acgatgcatc cacaaacgct gaaattgctc aattcaataa agcaaagtgt
    19081 gcaacgcaaa tggcaccaga ttcaactttc aagatcgcat tatcacttat ggcatttgat
    19141 gcggaaataa tagatcagaa aaccatattc aaatgggata aaacccccaa aggaatggag
    19201 atctggaaca gcaatcatac accaaagacg tggatgcaat tttctgttgt ttgggtttcg
    19261 caagaaataa cccaaaaaat tggattaaat aaaatcaaga attatctcaa agattttgat
    19321 tatggaaatc aagacttctc tggagataaa gaaagaaaca acggattaac agaagcatgg
    19381 ctcgaaagta gcttaaaaat ttcaccagaa gaacaaattc aattcctgcg taaaattatt
    19441 aatcacaatc tcccagttaa aaactcagcc atagaaaaca ccatagagaa catgtatcta
    19501 caagatctgg ataatagtac aaaactgtat gggaaaactg gtgcaggatt cacagcaaat
    19561 agaaccttac aaaacggatg gtttgaaggg tttattataa gcaaatcagg acataaatat
    19621 gtttttgtgt ccgcacttac aggaaacttg gggtcgaatt taacatcaag cataaaagcc
    19681 aagaaaaatg cgatcaccat tctaaacaca ctaaatttat aaaaaatcta atggcaaaat
    19741 cgcccaaccc ttcaatcaag tcgggacggc caaaagcaag cttttggctc ccctcgctgg
    19801 cgctcggcgc cccttatttc aaacgttaga cggcaaagtc acagaccgcg ggatctc
//
