LOCUS       NZ_CP058764             2022 bp    DNA     circular CON 02-MAY-2024
DEFINITION  Klebsiella pneumoniae strain STLIN_3 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP058764
VERSION     NZ_CP058764.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925574
            Assembly: GCF_022494395.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. Unicycler 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494395.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 05:13:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,437
            CDSs (total)                      :: 5,313
            Genes (coding)                    :: 5,144
            CDSs (with protein)               :: 5,144
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 55 of 169
            Pseudo Genes (incomplete)         :: 110 of 169
            Pseudo Genes (internal stop)      :: 25 of 169
            Pseudo Genes (multiple problems)  :: 19 of 169
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058764.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="STLIN_3"
                     /isolation_source="Stanley WWTPs_3 WWTPs influent"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.2 N 114.2 E"
                     /collection_date="2018-12"
     gene            complement(join(2003..2022,1..172))
                     /locus_tag="HZT06_RS27105"
                     /old_locus_tag="HZT06_27055"
     CDS             complement(join(2003..2022,1..172))
                     /locus_tag="HZT06_RS27105"
                     /old_locus_tag="HZT06_27055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1203..1319
                     /locus_tag="HZT06_RS27110"
                     /old_locus_tag="HZT06_27060"
     CDS             1203..1319
                     /locus_tag="HZT06_RS27110"
                     /old_locus_tag="HZT06_27060"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_240726601.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKVISRVFRSKR"
                     /name="small membrane protein"
     Typing          48..667
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.086"
                     /coverage="99.0"
                     /evalue="5.48e-173"
ORIGIN
        1 cattcaggcg cgctgacagg cttcggtaaa ggcgttccgc atcctcatgg agtttctcgc
       61 agaaatcagc ctcatgatcc agatccagct cattaagcct gtccagaaga agcagggact
      121 gatcctggat gaatttagcc atgttaacgg cagtcttttg ttgtttgttc atgaaggccc
      181 cggcgcgaaa gtgcttactg tacgttctga tggttttccg cttcctcgct cattcgctca
      241 ctccgttcgg tcatgagtct gcggcgagcg gagggaggat aagcagataa cgtaacggta
      301 agcataacga aagctaagga cgagggcgaa aaggccaggc cccggcgggg cgtttttcca
      361 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
      421 cccgacagga ctataaagat accaggcgtt tccccctggt ggctccctcg tgcgctctcc
      481 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
      541 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
      601 cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      661 aaagacatgc aaatgcgcca ctggcagcag ccactggtaa caggatgtgc gagagatatt
      721 gtgagagttc ttgaagtggt gcccttacta cggctacact agaaggacag cttgggtgac
      781 acgtctcgca taagactgtt accacggttc agaagttccc caacttactg aaccttcgaa
      841 aaaccgcctt gcagggcggt tttttcgttt acagagaaag agattacgac gaggctgaat
      901 gaatctcaag atttcattta gtctgccacc cgatcattca aagttttggg gtagttctga
      961 cctgcacccc gtgatttgtt atatacccac cacgggggca ttagtttttt ttatttttat
     1021 tttgatgata tgtacaaaat cagtgattaa gaataatctt aacggcctta acaaacacat
     1081 acaaaaacag cggttatgtt tcatctctcc acaataagta acgacagtat catgctaaca
     1141 aaagatgata ttatctttat gaaatcataa aaggctgttt ttattaaatt aatcaggcat
     1201 ttatggactg gatttcactt gctatcgcca ttgctctact tgttgtagct gtctactcag
     1261 gaatctcata cttcagagaa aaaaaagtaa tcagtcgtgt atttaggagt aagcgatagc
     1321 gccctattta ccataatccc ttcttacgcg caccgcgaaa acggcctcag ggcgtttctg
     1381 acgggaaacc ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa cgcgcaggct
     1441 ctgtttttac ggcatgcatg agtatgcgcg caagtgcata gcgcggaaat tcacgcaact
     1501 cagccggggt aagtcctgtt tccggcattc ttcccacccg aaaacggtga acgggaacgc
     1561 aatttattgc gtggccgtga ggcgttgccg gcacagccgg aaaaaccgtt ttcagcacgt
     1621 tcaggaaggg ggtcggatgt acaaatggca cagaggatgg aactgacgta aaagtgacgt
     1681 atgagctgaa ggagcaatgt aattctgtag tcaggaagaa taacccgttc atgcaggcat
     1741 gaacgataca gtgaggaacg ctttttttgt cacgttctcc ccttaacctg cgtaaatcac
     1801 ccgccatggt tgattatttt tcaacctcaa aatggtcata cacctaacgc agccgcgatt
     1861 ttcatcgcaa cttcatgaaa aataaataaa aaagaaaaaa tacagggatt gtgggtatta
     1921 ccagggtaat aatgttaagc gctaagacaa tgaaaattaa ggcgatgctg gtattactcc
     1981 ggtaataccc agaacggaga cattacgcac cgtcctgctg ct
//
