LOCUS       NZ_CP058763             3300 bp    DNA     circular CON 02-MAY-2024
DEFINITION  Klebsiella pneumoniae strain STLIN_3 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP058763
VERSION     NZ_CP058763.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925574
            Assembly: GCF_022494395.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. Unicycler 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494395.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 05:13:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,437
            CDSs (total)                      :: 5,313
            Genes (coding)                    :: 5,144
            CDSs (with protein)               :: 5,144
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 55 of 169
            Pseudo Genes (incomplete)         :: 110 of 169
            Pseudo Genes (internal stop)      :: 25 of 169
            Pseudo Genes (multiple problems)  :: 19 of 169
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058763.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="STLIN_3"
                     /isolation_source="Stanley WWTPs_3 WWTPs influent"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.2 N 114.2 E"
                     /collection_date="2018-12"
     gene            join(2120..3300,1..352)
                     /locus_tag="HZT06_RS27100"
                     /old_locus_tag="HZT06_27045"
     CDS             join(2120..3300,1..352)
                     /locus_tag="HZT06_RS27100"
                     /old_locus_tag="HZT06_27045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="WP_193951167.1"
                     /translation="MKKNVVRQVLEMSGPCISSDLAERIQWQHPSMSPEAIRKMISRST
                     DIGKLPFLKFSHNRRFIYLKDDFGSFKFWRALEKCMYEANSTYSHAILAVINNGGYLKV
                     KDFGIVSGSPIKQAKHLSYETVLRNLLSAKILRAVYIDGVGDCVLINNNIANDVNIRTM
                     ANCESFFDKPILELVKAWLRNLGLVAFNQIKTKYDGEGNPVVGSFEWDMTAPSYVSPLA
                     EYVGGKLMPGFVACDFSLGFNRDEITTAAAETFIRKVQMTKSSRASQRIMFVIFARRFG
                     KIAFNKLRSEGVLAVTIANAFGNKVDESLTRLSRVVQGSLSIEKHPDELLQMVKDLESV
                     SGENGNLRGYIFELFVSSQISNFYGLGNIYINREYKINGKHAEADVVLESSDDIYIIEC
                     KNVKVLPSTELTRWMKERVPTINSYYKINNLEKKNIHHYLWVTGNVYNKDLKRLDSFKN
                     NNKNIDVDYLFGEHLDDFFYKKKRVFNLYRKVISPDYKKGVMPDFELEDDFF"
                     /name="NERD domain-containing protein"
     gene            complement(541..699)
                     /locus_tag="HZT06_RS27240"
     CDS             complement(541..699)
                     /locus_tag="HZT06_RS27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001403044.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHDKTPLAAAIGIWFTERRGV
                     YKHTQSS"
                     /name="hypothetical protein"
     Typing          complement(401..881)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.687"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 ttatataata gaatgcaaaa atgttaaggt cttaccttca actgaactta cgcgttggat
       61 gaaagaaagg gttccaacta taaactctta ctataaaata aataatttag aaaaaaagaa
      121 tatccaccat tatttgtggg ttacaggtaa tgtgtacaac aaagatttaa aaaggttaga
      181 ctcttttaag aataataata agaatatcga tgttgattat ttgtttggtg agcatttgga
      241 tgatttcttt tacaagaaaa agcgggtgtt caacttatat agaaaagtca tttcacctga
      301 ttataaaaaa ggcgtaatgc ctgattttga gttagaagac gattttttct agctactgcg
      361 tttcttgacg atggcgcttt gaaaggcgta tcttccatct ctgaaacgaa aaaaccaccc
      421 ggcaaggtgg tttttcgaag gttcaggaaa ccggcaagtt tctgaaccgt ggtaactggc
      481 ttaaacggag cgcagtgacc aaatctgtcc ttccagtgta gccgtagtca ggtcaacact
      541 tcaagaactc tgtgtatgct tgtacacgcc tctccgctcc gtaaaccaga taccaatggc
      601 tgctgccagt ggcgttttgt cgtgtctttc cgggttggac tcaagtcgat agttaccgga
      661 acaggcgcag cagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
      721 tgccttcccg gaactgagtg tcaggcgtgg aatgagataa acgcggccat aacagcggag
      781 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg cctatcagaa
      841 atgataggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag tgtcagattt
      901 cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ccgctgtgcg gcctttcctt
      961 ttccacatca cgcgaccgct cggttttctc gagcgtttct acgcacgctc ctgccgctcg
     1021 ccgcagccga acggcaggag cgtagcgact gagtgagcga ggaagcggaa aagagtctgg
     1081 ttttacattg agcactgatg ctccgaggcc ccttatgact aaacaacaga agaccgcgct
     1141 gaatatggca aaatatatcc agagccagtc cctgttttcc tgtccggcca tcccggggag
     1201 ttcctgaacc ttcccaaaaa tcggcagatc tctgaacgct cctcaccacg aaaaataccc
     1261 acacactacg caaaattaaa attttgcaga tttttattta atatcagtgg tttaccttcc
     1321 tgaaatttcg ttttttaaat tttatttata tagatccgac tctatgacga ctcgtcatag
     1381 agtccccggc gagtcgttat agagtcggta acgactcaga actataaaaa caataagtta
     1441 ataacgaccc gactctagac ccgactcccc gtttggttgt tttttagacg cggcgactct
     1501 gggtacgact ctagtgaaga gtcgccggtg aatcgtgtgg taagtcgtcc ggcgacttgc
     1561 cataacgtga tgctggctgt cgacgaaccc gattcctgac cgtgctgaaa acggtttttc
     1621 cggctgcgcc ggcaacgctt cacggccacg caataaattg cgtccccgtt caccgttttc
     1681 ggagtagaag aacgccggaa acaggactta ccttggctga atggtgtcaa tttccgcgct
     1741 atgcacttgt gcgcatactc atgcatgccg taaaaacaga gcctgtgcgt ttctggcggg
     1801 ttttcgggtg gtttgttgcc tgttttaccg gtttcccgtc agaaacgctc tgagggcctc
     1861 tcaggcggtg ctgataacgg atgttatggt aaatggagta gctgatagtt cgataaaaaa
     1921 atgtcacaaa actatcttta tgatttaaaa ggattaaata attataaaga cgatatttct
     1981 ttgtgcttaa tttaaagtaa gtaattaacc atatgatttt aaattgtttt ttatagtcac
     2041 tttttttaaa aagactgcgc gccattacac gtagtgtaat gttataaata gcgtagtgtg
     2101 acagaaataa ggtgaggcta tgaaaaaaaa tgttgttaga caagtacttg aaatgagcgg
     2161 gccatgcatc tcttcagatc ttgcagagag aattcagtgg caacatccct caatgtcccc
     2221 tgaagcgata cgtaaaatga tctcgcgaag cacggacata ggtaagctgc catttttgaa
     2281 attcagccat aacaggagat tcatttatct aaaagatgat tttggcagtt tcaaattttg
     2341 gcgagctctc gaaaagtgca tgtacgaagc aaactcaact tactcacacg cgatacttgc
     2401 cgtaataaat aatggcggtt atttgaaggt gaaagatttt gggattgtgt ctggatctcc
     2461 tataaaacaa gctaaacatt taagttatga aacagttcta agaaatctac tttcggcaaa
     2521 aatacttaga gctgtgtata ttgatggagt tggtgattgc gtactgataa ataacaacat
     2581 agccaacgat gttaatatta gaacaatggc aaattgcgaa tccttttttg acaaaccaat
     2641 attggagctt gttaaagctt ggttgaggaa tttaggccta gtcgctttta atcagataaa
     2701 aacaaaatat gatggggagg gaaatccagt tgtaggctct tttgaatggg atatgaccgc
     2761 tccaagttac gtttcccctc tcgctgaata tgtcggcgga aaactgatgc ctggtttcgt
     2821 tgcctgtgat ttttctctcg gctttaatcg tgatgaaatt actaccgctg cagcagaaac
     2881 attcataaga aaagtccaga tgactaagtc ttctcgagcg agtcagagga taatgtttgt
     2941 tattttcgca aggcgtttcg gcaaaatagc cttcaataag ttgagaagtg agggggttct
     3001 ggctgtaact atagcaaatg cgtttggtaa taaagttgat gaaagcttaa ccaggttgtc
     3061 gagggtggtt cagggttctt tgtccattga aaagcatcca gatgagttgc ttcagatggt
     3121 taaagatctg gagtctgttt ctggagagaa tggcaatttg agagggtata tttttgagct
     3181 ttttgtatcc tcgcaaattt ctaattttta tggtcttggt aatatttata ttaatagaga
     3241 gtataagata aatggtaagc atgcagaagc tgatgttgta ttagagagta gtgacgatat
//
