LOCUS       NZ_CP058757             4593 bp    DNA     circular CON 02-MAY-2024
DEFINITION  Klebsiella pneumoniae strain STLIN_19 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP058757
VERSION     NZ_CP058757.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925573
            Assembly: GCF_022494375.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. Unicycler 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494375.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 05:10:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,627
            CDSs (total)                      :: 5,505
            Genes (coding)                    :: 5,327
            CDSs (with protein)               :: 5,327
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 77 of 178
            Pseudo Genes (incomplete)         :: 106 of 178
            Pseudo Genes (internal stop)      :: 31 of 178
            Pseudo Genes (multiple problems)  :: 33 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058757.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="STLIN_19"
                     /isolation_source="Stanley WWTPs_19 WWTPs influent"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.2 N 114.2 E"
                     /collection_date="2018-12"
     gene            complement(561..677)
                     /locus_tag="HZT05_RS28050"
                     /old_locus_tag="HZT05_27920"
     CDS             complement(561..677)
                     /locus_tag="HZT05_RS28050"
                     /old_locus_tag="HZT05_27920"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_240726601.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKVISRVFRSKR"
                     /name="small membrane protein"
     gene            1707..1898
                     /locus_tag="HZT05_RS28055"
                     /old_locus_tag="HZT05_27925"
     CDS             1707..1898
                     /locus_tag="HZT05_RS28055"
                     /old_locus_tag="HZT05_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_240726602.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     EHLFRTLSLRLDALQKGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1898..2302
                     /locus_tag="HZT05_RS28060"
                     /old_locus_tag="HZT05_27930"
     CDS             1898..2302
                     /locus_tag="HZT05_RS28060"
                     /old_locus_tag="HZT05_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_023301406.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRVFGAFAPVPVEQRGEQVSGQRDTVTSDRNEQVIYILKQEIEHLNTLLASRDSHIDS
                     LKQAMLLLENREEKRQNTTRPRAWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2309..2935)
                     /locus_tag="HZT05_RS28065"
                     /old_locus_tag="HZT05_27935"
     CDS             complement(2309..2935)
                     /locus_tag="HZT05_RS28065"
                     /old_locus_tag="HZT05_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_240726600.1"
                     /translation="MGVYYPDASNPQLFTGWKAVTELGPAILATAIAATAFSLWLFILF
                     FRSSESRRIQSETDRYKQALREAEEAHRARQNTLIQNHNLETAALRSHYSGAEDVLREE
                     FAKKENRWRQALQNAREEARQELEEQTEALNQRASGLLVQEQTLKARMEEVRKYVAGER
                     HNAMRARLEAEEAEKRRQNASATAERRRRKLEKLQATDGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(3004..3891)
                     /locus_tag="HZT05_RS28070"
                     /old_locus_tag="HZT05_27940"
     CDS             complement(3004..3891)
                     /locus_tag="HZT05_RS28070"
                     /old_locus_tag="HZT05_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023301404.1"
                     /translation="MAKFASAARQATGVIKILQGDILRSVGTVRNYEQGLTRVAEWMKD
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAPDT
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="hypothetical protein"
     gene            complement(3884..4105)
                     /locus_tag="HZT05_RS28075"
                     /old_locus_tag="HZT05_27945"
     CDS             complement(3884..4105)
                     /locus_tag="HZT05_RS28075"
                     /old_locus_tag="HZT05_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162091.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     Typing          complement(1214..1832)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.94"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgcctgcccg gtcaccgttt cacatcatgg cgaggaggcg ggatctgaaa cggtgtgatg
       61 aaagtgcaga tgaggtcaga cgatgcaggt cggtgagtcc gtggtgaacg cgatagtgga
      121 ataccacgca cagggcagtg aggaggcgca tgcagaggaa aacaggatgt tcctgcgtta
      181 tctgcgcgag ttaacgggaa cacagcgtgt ggcggttgag gtggccagga aatatttttc
      241 gccggtgagg ggctgatatg agctaccttt ttcttgtcgc cggcaccgca ccaggtgcga
      301 cggtcctttt acagagttgt cctcatttga cgggcctgga tggcccgttc ttttatgctt
      361 ttaacgtccg gggccctttt ctcgaccgaa atcacctact atgcagtggt gtgaatagcc
      421 atgcatatgc gtaaaataaa tcaggcgcgt ttctggcggt tttcggtgta cttgccacca
      481 ccttttgccc gttaaccacc agaaacgccc tgaggccgtt ttcgcggtgc gcgtaagaag
      541 ggattatggt aaatagggcg ctatcgctta ctcctaaata cacgactgat tacttttttt
      601 tctctgaagt atgagattcc tgagtagaca gctacaacaa gtagagcaat ggcgatagca
      661 agtgaaatcc agtccataaa tgcctgatta atttaataaa aacagccttt tatgatttca
      721 taaagataat atcatctttt gttagcatga tactgtcgtt acttattgtg gagagatgaa
      781 acataaccgc tgtttttgta tgtgtttgtt aaggccgtta agattattct taatcactga
      841 ttttgtacat atcatcaaaa taaaaataaa aaaaactaat gcccccgtgg tgggtatata
      901 acaaatcacg gggtgcaggt cagaactacc ccaaaacttt gaatgatcgg gtggcagact
      961 aaatgaaatc ttgagattca ttcagcctcg tcgtaatctc tttctctgta aacgaaaaaa
     1021 ccgccctgca aggcggtttt tcgaaggttc agtaagttgg ggaacttctg aaccgtggta
     1081 acagtcttat gcgagacgtg tcacccaagc tgtccttcta gtgtagccgt agtaagggca
     1141 ccacttcaag aactctcaca atatctctcg cacatcctgt taccagtggc tgctgccagt
     1201 ggcgcatttg catgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag
     1261 cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg
     1321 gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt
     1381 aaaccgaaag gcaggaacag gagagcgcac gagggagcca ccagggggaa acgcctggta
     1441 tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt
     1501 gtcagggggg cggagcctat ggaaaaacgc cccgccgggg ccttctcccg tctgttcttt
     1561 tcccttcccg ggtggtggct atgacttttc tgtctgtgtg ccctgtccgc tcgccgcagc
     1621 cgaacggcag gagcgtagcg actgagtgag cgaggaagcg gaaaaccatc agaacgtaca
     1681 gtaagcactt tcgcgccggg gccttcatga acaaacaaca aaagactgcc gttaacatgg
     1741 ctaaattcat ccaggatcag tccctgcttc ttctggacag gcttaatgag ctggatctgg
     1801 atcatgaggc tgatttctgc gagaaactcc atgaggatgc ggagcatctt ttccgtacac
     1861 tgtccctgag actggatgca ctacagaagg gtgactgatg gcgaaagtgt cgatcagtga
     1921 ggcagcacgc ctgacgggta agagcaggac aaccctgcac aggttaatta aagccggtga
     1981 actgtccacc tgtcggggtg aacggaatgc gaaaatgctc gatacttcag agcttttgcg
     2041 ggtttttggt gcctttgcgc ctgtacccgt tgaacagagg ggtgaacagg tcagtggaca
     2101 gcgtgacaca gtcacttctg accgtaatga acaggtcatt tacattctga aacaggaaat
     2161 tgaacacctg aacaccctcc ttgcctccag agattcacat atcgacagcc tgaaacaggc
     2221 gatgttgttg ctggagaacc gggaagagaa gcggcagaac acgacccggc cccgtgcctg
     2281 gtggcgtttc tggcagtcct gaaatgcctc agtgtttttg ccctgctgtt ccgtcagtgg
     2341 cctggagttt ttccagtttc cgtctgcgcc gttccgctgt tgcgctggca ttttgtcgcc
     2401 ttttctctgc ttcctctgcc tccagacggg cccgcatggc gttatgccgc tctccggcaa
     2461 catactttct gacttcctcc attctggctt tcagtgtctg ctcctgtacc agcaggccgg
     2521 acgcgcgctg attcagtgcc tccgtctgtt cctccagctc ctgcctggct tcttcccggg
     2581 cattctgcaa cgcctgacgc cagcggtttt cttttttcgc aaactcttcc cgcagtacat
     2641 cctcagcacc actgtagtgg cttcgcaggg ccgctgtctc cagattgtga ttctgaataa
     2701 gggtattttg tctggctctg tgagcctctt ctgcctccct cagagcctgc ttatacctgt
     2761 ctgtctcgct ctgtatccgc cgggactctg agcttctgaa aaacaaaatg aacagccaca
     2821 gcgagaacgc tgtggctgcg atcgcggtag ccaggattgc gggaccgagt tcggtgaccg
     2881 ccttccagcc agtgaacagc tgcggattgc tggcatccgg atagtaaacg cccatatgtt
     2941 tcagctcata cagggcgtac gcgatggcga aaacaccggt acacagtaaa ataaacgttc
     3001 ttgtcatcgc aggtatgtct ccgtgatttc cggacgaaaa tggcccattt cctggctgac
     3061 ggtacgcagg gccatatcac gaaccagacc gagtttttgc agctcctgca tccgttgctg
     3121 ggcgtagctg tgacgcacgc cgtgtgcccc ccgtgaccag cccagggtac gcatggaggc
     3181 ggcactgaaa gcgttcgacc agcgctgtcc tccgccaatg gcgtactgac tctggtaaaa
     3241 cacgccgcga tcggtcaccc ggaccgggct cgccagcctg acatgctcca gacgttcagc
     3301 caggcgtgac ggcagcagaa cttccctgac caggccgcct ttgccctgaa cggtgtaaat
     3361 cacaccctcc cgaccggcaa atttactgtc cgctgccgga cgggtatctg gtgcacgctc
     3421 ctggcggggc gcgagggtca gcagttcatg ggcgcgtaat ccggcggcca gagcaatcgc
     3481 ggtggccaga gcgttggcgg gctgctgtgc agcggcaatc agggccacct ggtccgggct
     3541 gtaggcccgg ctgctcagga tctgtgcctg ctccgatttc accaccggca ggtgttcgcc
     3601 ggcagggagg gtctgcgtca cgtggatcat catggcctgg atggcctggc gttccatatc
     3661 gagcgttttc tggccgacct gctccccgcg ctgctccagg taggtgatgg cctgcgccgg
     3721 tgtcagtgtg cgtaaatcgc cgtgattaat ccggttgtct ttcatccatt ctgcgacgcg
     3781 ggtcaggccc tgctcgtaat tccggactgt gccgacagag cgcagaatat cgccctggag
     3841 gatttttatc acgcccgtcg cctggcgggc agcgctggcg aatttagcca tttttttcca
     3901 gccagcgggt aaccgtggac aacacgactt tgattcgccg ggcccgtagc cagcgttgca
     3961 gctccgctgc actggccccg gcctgataca ggctcagtag ttcgccggta tagcggtcaa
     4021 ggcgggaggg acggtagcgg acctggcgga tacggcgggt ctgcgcctgc agggcggcca
     4081 gctcggcagc ggtatcaaat tccacaggaa ctccggtgtt gagttgtaag aacgccagtg
     4141 gaaatccact gcggcctgcg ccgatcgccc tcgtttaaac cgtaacgtgg ttaagcccgg
     4201 actgctcgcg gctcaacgtc tctttagtca gagaatgact tgacccggtt gtgggtctga
     4261 cagatttggg ctgttttctc agagttctcg gattgggctg cccggtacag tatccccgga
     4321 gggacccggc tgttttgcct gctgccggta ggcaggtgcg cgtcagagtg cgccttctca
     4381 tggtttggct aaatactagc caggtttttc gtgcgtgtga atgttgtttt ttaaatgttg
     4441 cgcctgactc agtttaatag cgacacccgc tatgtacagg cgcattcatg cgtcgtacat
     4501 agcgggtgtc gcaggtgctg atttggcgga ataaaaaaca ccgggactga ccgccccgga
     4561 ccccgccaac ggtgaccgga cagacaaaaa att
//
