LOCUS       NZ_CP058747             4588 bp    DNA     circular CON 30-APR-2024
DEFINITION  Klebsiella pneumoniae strain STLIN_10 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP058747
VERSION     NZ_CP058747.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925567
            Assembly: GCF_022494255.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. Unicycler 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494255.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 04:30:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,530
            CDSs (total)                      :: 5,408
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 56 of 145
            Pseudo Genes (incomplete)         :: 96 of 145
            Pseudo Genes (internal stop)      :: 22 of 145
            Pseudo Genes (multiple problems)  :: 27 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058747.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="STLIN_10"
                     /isolation_source="Stanley WWTPs_10 WWTPs influent"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.2 N 114.2 E"
                     /collection_date="2018-12"
     gene            324..515
                     /locus_tag="HZS96_RS27580"
                     /old_locus_tag="HZS96_27460"
     CDS             324..515
                     /locus_tag="HZS96_RS27580"
                     /old_locus_tag="HZS96_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023301407.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQKGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            515..919
                     /locus_tag="HZS96_RS27585"
                     /old_locus_tag="HZS96_27465"
     CDS             515..919
                     /locus_tag="HZS96_RS27585"
                     /old_locus_tag="HZS96_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_023301406.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRVFGAFAPVPVEQRGEQVSGQRDTVTSDRNEQVIYILKQEIEHLNTLLASRDSHIDS
                     LKQAMLLLENREEKRQNTTRPRAWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(926..1552)
                     /locus_tag="HZS96_RS27590"
                     /old_locus_tag="HZS96_27470"
     CDS             complement(926..1552)
                     /locus_tag="HZS96_RS27590"
                     /old_locus_tag="HZS96_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_240726600.1"
                     /translation="MGVYYPDASNPQLFTGWKAVTELGPAILATAIAATAFSLWLFILF
                     FRSSESRRIQSETDRYKQALREAEEAHRARQNTLIQNHNLETAALRSHYSGAEDVLREE
                     FAKKENRWRQALQNAREEARQELEEQTEALNQRASGLLVQEQTLKARMEEVRKYVAGER
                     HNAMRARLEAEEAEKRRQNASATAERRRRKLEKLQATDGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(1621..2508)
                     /locus_tag="HZS96_RS27595"
                     /old_locus_tag="HZS96_27475"
     CDS             complement(1621..2508)
                     /locus_tag="HZS96_RS27595"
                     /old_locus_tag="HZS96_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023301404.1"
                     /translation="MAKFASAARQATGVIKILQGDILRSVGTVRNYEQGLTRVAEWMKD
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAPDT
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="hypothetical protein"
     gene            complement(2501..2722)
                     /locus_tag="HZS96_RS27600"
                     /old_locus_tag="HZS96_27480"
     CDS             complement(2501..2722)
                     /locus_tag="HZS96_RS27600"
                     /old_locus_tag="HZS96_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162091.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     Typing          complement(2..289)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.946"
                     /coverage="92.0"
                     /evalue="1.03e-70"
     Typing          complement(4192..4588)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.333"
                     /coverage="94.0"
                     /evalue="5.14e-98"
ORIGIN
        1 gaaaggcagg aacaggagag cgcacgaggg agccaccagg gggaaacgcc tgggatcttt
       61 atagtcctgt cgggtttcgc caccgctgat ttgagcgtca gattttgtga tgcttgtcag
      121 gggggcggag cctatggaaa aacggcaacg ccgtggcctt ctcccgtctg ttcttttccc
      181 ttcccgggtg gtggctatga cttttctgtc tgtgtgccct gtccgctcgc cgcagccgaa
      241 cggctggagc gtagcgacgg agtgagcgag gaagcggaaa agagaccggt tttacactga
      301 gcactgacgc cctgaggccc tttatgacga aacaacagaa aactgcgctg aacatggcta
      361 aattcatcca gaatcagtcc ctgctcctgc tggagaagct taacgagctt gatctcgatg
      421 cggaggctga cctttgtgag aaactccacg atgatgcgga gcatcttttc cgtacactgt
      481 ccctgagact ggatgcacta cagaagggtg actgatggcg aaagtgtcga tcagtgaggc
      541 agcacgcctg acgggtaaga gcaggacaac cctgcacagg ttaattaaag ccggtgaact
      601 gtccacctgt cggggtgaac ggaatgcgaa aatgctcgat acttcagagc ttttgcgggt
      661 ttttggtgcc tttgcgcctg tacccgttga acagaggggt gaacaggtca gtggacagcg
      721 tgacacagtc acttctgacc gtaatgaaca ggtcatttac attctgaaac aggaaattga
      781 acacctgaac accctccttg cctccagaga ttcacatatc gacagcctga aacaggcgat
      841 gttgttgctg gagaaccggg aagagaagcg gcagaacacg acccggcccc gtgcctggtg
      901 gcgtttctgg cagtcctgaa atgcctcagt gtttttgccc tgctgttccg tcagtggcct
      961 ggagtttttc cagtttccgt ctgcgccgtt ccgctgttgc gctggcattt tgtcgccttt
     1021 tctctgcttc ctctgcctcc agacgggccc gcatggcgtt atgccgctct ccggcaacat
     1081 actttctgac ttcctccatt ctggctttca gtgtctgctc ctgtaccagc aggccggacg
     1141 cgcgctgatt cagtgcctcc gtctgttcct ccagctcctg cctggcttct tcccgggcat
     1201 tctgcaacgc ctgacgccag cggttttctt ttttcgcaaa ctcttcccgc agtacatcct
     1261 cagcaccact gtagtggctt cgcagggccg ctgtctccag attgtgattc tgaataaggg
     1321 tattttgtct ggctctgtga gcctcttctg cctccctcag agcctgctta tacctgtctg
     1381 tctcgctctg tatccgccgg gactctgagc ttctgaaaaa caaaatgaac agccacagcg
     1441 agaacgctgt ggctgcgatc gcggtagcca ggattgcggg accgagttcg gtgaccgcct
     1501 tccagccagt gaacagctgc ggattgctgg catccggata gtaaacgccc atatgtttca
     1561 gctcatacag ggcgtacgcg atggcgaaaa caccggtaca cagtaaaata aacgttcttg
     1621 tcatcgcagg tatgtctccg tgatttccgg acgaaaatgg cccatttcct ggctgacggt
     1681 acgcagggcc atatcacgaa ccagaccgag tttttgcagc tcctgcatcc gttgctgggc
     1741 gtagctgtga cgcacgccgt gtgccccccg tgaccagccc agggtacgca tggaggcggc
     1801 actgaaagcg ttcgaccagc gctgtcctcc gccaatggcg tactgactct ggtaaaacac
     1861 gccgcgatcg gtcacccgga ccgggctcgc cagcctgaca tgctccagac gttcagccag
     1921 gcgtgacggc agcagaactt ccctgaccag gccgcctttg ccctgaacgg tgtaaatcac
     1981 accctcccga ccggcaaatt tactgtccgc tgccggacgg gtatctggtg cacgctcctg
     2041 gcggggcgcg agggtcagca gttcatgggc gcgtaatccg gcggccagag caatcgcggt
     2101 ggccagagcg ttggcgggct gctgtgcagc ggcaatcagg gccacctggt ccgggctgta
     2161 ggcccggctg ctcaggatct gtgcctgctc cgatttcacc accggcaggt gttcgccggc
     2221 agggagggtc tgcgtcacgt ggatcatcat ggcctggatg gcctggcgtt ccatatcgag
     2281 cgttttctgg ccgacctgct ccccgcgctg ctccaggtag gtgatggcct gcgccggtgt
     2341 cagtgtgcgt aaatcgccgt gattaatccg gttgtctttc atccattctg cgacgcgggt
     2401 caggccctgc tcgtaattcc ggactgtgcc gacagagcgc agaatatcgc cctggaggat
     2461 ttttatcacg cccgtcgcct ggcgggcagc gctggcgaat ttagccattt ttttccagcc
     2521 agcgggtaac cgtggacaac acgactttga ttcgccgggc ccgtagccag cgttgcagct
     2581 ccgctgcact ggccccggcc tgatacaggc tcagtagttc gccggtatag cggtcaaggc
     2641 gggagggacg gtagcggacc tggcggatac ggcgggtctg cgcctgcagg gcggccagct
     2701 cggcagcggt atcaaattcc acaggaactc cggtgttgag ttgtaagaac gccagtggaa
     2761 atccactgcg gcctgcgccg atcgccctcg tttaaaccgt aacgtggtta agcccggact
     2821 gctcgcggct caacgtctct ttagtcagag aatgacttga cccggttgtg ggtctgacag
     2881 atttgggctg ttttctcaga gttctcggat tgggctgccc ggtacagtat ccccggaggg
     2941 acccggctgt tttgcctgct gccggtaggc aggtgcgcgt cagagtgcgc cttctcatgg
     3001 tttggctaaa tactagccag gtttttcgtg cgtgtgaatg ttgtttttta aatgttgcgc
     3061 ctgactcagt ttaatagcga cacccgctat gtacaggcgc attcatgcgt cgtacatagc
     3121 gggtgtcgca ggtgctgatt tggcggaata aaaaacaccg ggactgaccg ccccggaccc
     3181 cgccaacggt gaccggacag acaaaaaatt tgcctgcccg gtcaccgttt cacatcatgg
     3241 cgaggaggcg ggatctgaaa cggtgtgatg aaagtgcaga tgaggtcaga cgatgcaggt
     3301 cggtgagtcc gtggtgaacg cgatagtgga ataccacgca cagggcagtg aggaggcgca
     3361 tgcagaggaa aacaggatgt tcctgcgtta tctgcgcgag ttaacgggaa cacagcgtgt
     3421 ggcggttgag gtggccagga aatatttttc gccggtgagg ggctgatatg agctaccttt
     3481 ttcttgtcgc cggcaccgca ccaggtgcga cggtcctttt acagagttgt cctcatttga
     3541 cgggcctgga tggcccgttc ttttatgctt ttaacgtccg gggccctttt ctcgaccgaa
     3601 atcacctact atgcagtggt gtgaatagcc atgcatatgc gtaaaataaa tcaggcgcgt
     3661 ttctggcggt tttcggtgta cttgccacca ccttttgccc gttaaccacc agaaacgccc
     3721 tgaggccgtt ttcgcggtgc gcgtaagaag ggattatggt aaatagggcg ctatcgccta
     3781 aatgtacgac tgattgtttt tttttctctg aagtatgaga ttcctgagta gacagctaca
     3841 acaagtagag caatggcgat agcaagtaaa atccagtcca taaatgcctg attaatttaa
     3901 taaaaacagc ctgttatgat ttcgtaaaga taatatcatc tttttttagc atgatactgt
     3961 cgttacttat tgtggtgaga tgaaacataa ccgctgtttt tgtatgtgtt tgttaaggcg
     4021 gctaagatta ttcctaacca ctgattttgt acatatcatc aaaaaaataa aaaaactaat
     4081 gccctgtggt gggtatgtaa caaatcacgg ggagcgggtc agaactacgc caaaactttg
     4141 aatgatcggg tggcaggcta aatgaaatct tgagattcat tcagcctcgt cgtaatctct
     4201 tgctctgtaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttca gcaagttggg
     4261 gaacttctga accgtggtaa cagggtgtac aagaccgctg ccaccaaata cgtccttcca
     4321 gtttagccgt agttaggctt caacttcaag aactctgtac cagttatctc ttgtacaccc
     4381 ctttaccagt ggctaccgcc aatggcgatt tgtcgtgcct tttcgggttg gactcaagac
     4441 gatagttacc gaaaagagcg tggtagtcgg actgaacggg gggttcgtgc atacagtcca
     4501 gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga taaacgcggc
     4561 cataacagcg gaatgacacc ggtaaacc
//
