LOCUS       NZ_CP058724             4017 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Escherichia coli strain M159-1 plasmid pM159-1.5, complete sequence.
ACCESSION   NZ_CP058724
VERSION     NZ_CP058724.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15437881
            Assembly: GCF_022369015.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Wang,C., Zhao,J., Kang,Q., Ji,F., Wang,X. and Liu,Z.
  TITLE     Illegal smuggling Malayan pangolin as potential reservoirs for
            Multidrug-Resistant bacteria: An increasing public health risk
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Wang,C., Zhao,J., Kang,Q., Ji,F., Wang,X. and Liu,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2020) Guangdong Public Laboratory of Wild Animal
            Conservation and Utilization, Guangdong Institute of Applied
            Biological Resources, Guangdong Academy of Science, Microbial
            Pathogenic Ecology Group, 105 Xingang West Road, Guangzhou,
            Guangdong 510260, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-DEC-2018
            Assembly Method                   :: Canu v. V1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 101.36x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022369015.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 04:05:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,832
            CDSs (total)                      :: 4,708
            Genes (coding)                    :: 4,438
            CDSs (with protein)               :: 4,438
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 75 of 270
            Pseudo Genes (incomplete)         :: 184 of 270
            Pseudo Genes (internal stop)      :: 46 of 270
            Pseudo Genes (multiple problems)  :: 31 of 270
            Pseudo Genes (short protein)      :: 4 of 270
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058724.1.
            Bacteria and source DNA available from Guangdong Key Laboratory of
            Animal Conservation and Resource Utilization, Guangdong Public
            Laboratory of Wild Animal Conservation and Utilization, Guangdong
            Institute of Applied Biological Resources, Guangdong Academy of
            Science, Guangzhou, China.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="M159-1"
                     /isolation_source="Manis javanica"
                     /db_xref="taxon:562"
                     /plasmid="pM159-1.5"
                     /geo_loc_name="China:Guangzhou"
                     /lat_lon="23.16 N 113.23 E"
                     /collection_date="2018-12"
                     /collected_by="Guangzhou"
     gene            join(3993..4017,1..320)
                     /locus_tag="HZS86_RS23990"
     CDS             join(3993..4017,1..320)
                     /locus_tag="HZS86_RS23990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_016245707.1"
                     /translation="MAEKRNKMLTMWVTEDEHRRLLERCEGKQLAAWMRQICLDEKPSR
                     AGKLPSISPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAVLMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            529..>963
                     /locus_tag="HZS86_RS23995"
                     /pseudo=""
     CDS             529..>963
                     /locus_tag="HZS86_RS23995"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            998..1483
                     /locus_tag="HZS86_RS24000"
     CDS             998..1483
                     /locus_tag="HZS86_RS24000"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_061390563.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMISQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIQEQKSTQAMLSEKNNGVQLTTCGEERRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1487..1702
                     /locus_tag="HZS86_RS24005"
     CDS             1487..1702
                     /locus_tag="HZS86_RS24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1831..2358
                     /locus_tag="HZS86_RS24010"
     CDS             1831..2358
                     /locus_tag="HZS86_RS24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016246513.1"
                     /translation="MKCFWACSVSVVLAMSFTVAAAENNDNVTYTKPSAGRDLYLTCKG
                     YQVKGASVINVEKPSYLEGTGEQVEFAIITPVGASDGLKVFFDSWGFSSFGLIESHVMH
                     EHWSLVHVGGRASVKGEKIDYSFVDNQPQEGGSYKPKTGVFLYGCMNSDKRPSWVEKIY
                     TRTGVFRGGIED"
                     /name="hypothetical protein"
     gene            2613..2825
                     /locus_tag="HZS86_RS24015"
     CDS             2613..2825
                     /locus_tag="HZS86_RS24015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_034169353.1"
                     /translation="MGDFERTFGAGADFDRIIDENNSEYFREDRRKTKNSKKKFSSFQD
                     ALVWAKANPGKTIIRSPDGVGFIEK"
                     /name="hypothetical protein"
     gene            complement(3254..3367)
                     /locus_tag="HZS86_RS24225"
     CDS             complement(3254..3367)
                     /locus_tag="HZS86_RS24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072035093.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHDKTPLAAATGN"
                     /name="replication initiation protein"
     Typing          3724..3901
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.222"
                     /coverage="83.0"
                     /evalue="4.5e-50"
     Typing          complement(3255..3774)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.374"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          311..1794
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.717"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcactatgtg ggtgacagaa gacgagcacc gccgacttct ggagcgctgt gagggtaaac
       61 aactcgcggc ctggatgcgc cagatatgcc tggacgaaaa gccctcgcgt gctggcaaac
      121 ttccatccat ctcgccggcg ctgcttcgtc agcttgccgg catgggtaac aacctcaacc
      181 agattgcccg gcaggttaac gccggtggcg gtactgggca cgatcgcgtg caggttgtcg
      241 ccgtgctgat ggccattgat gccggactcg agcggctgcg gcacgccgtg ctggaaaagg
      301 gggcagacga tgatcgttaa gtttcatccc agaggccgcg gcggtggcgg cggtccggtg
      361 gattatctgc tggggaaaga ccggcagcgc gacggcgcca gcgttctgca gggtaagccg
      421 gaggaagtgc gggagcttat tgacgcctcg ccttacgcca agaaatacac ctccggcgtg
      481 ctgtcttttg ccgaacagga tttgccgccc ggccagcgtg aaaagctgat ggcgagcttt
      541 gagagggttc tgatgcccgg actcgataaa gaccagtaca gcgtactgtg ggttgagcac
      601 cgggacaaag ggcggctgga gctgaacttc ctgatcccga acacggagct gctgaccggc
      661 agacgtctcc agccgtatta cgaccgcgcc gaccgtccgc gcattgatgc ctggcagacc
      721 atagtgaacg gcagactggg gctgcacgac ccgaacgcgc cggaaaaccg gcgggcgctg
      781 gttacaccgt ccgcgctgcc ggaagcgaag caggaagccg cccaggcgat tacgcgcggc
      841 ctactttccc ttgcctcatc cggggagctg aaaacccgtc aggacgtcac tgaggcgctg
      901 gaaagtgcgg gttttgaggt ggtgcgcacc acgaaaagca gcatcagcat tgccgaccct
      961 gacggggggc gaaacatccg actgaaggga gccatctatg aacagtcttt taacgctggc
     1021 gaaggactta gagcagaaat cgaaagcgca gcagcagact accggcgaga tgctgaaagc
     1081 cgcattcagc gagcacgaga agtctgtcag agcggaactg agcgaaagcg agaagagaat
     1141 cagcgccgcc atcctcgacc acgaccggaa gctgtcctca gccatgagcc aacgcacgaa
     1201 agggatgctg cgtatgatca gccagacgtg gctgaccata gtcctggtct ccgcgctact
     1261 gatcgcctcg agcgcgggca ttctgtggtg gcaggggcaa cagatactcg acaactacac
     1321 gaccatccag gaacagaaga gcacgcaggc catgctttca gagaagaaca acggcgtcca
     1381 gctcacgacc tgcggggagg agagacgccg ctgcgtgagg gtgaacccgg aagcgggaag
     1441 attcggagag gattcgagct ggatgatact ggcggggaaa tagcacatga cggaactgga
     1501 aaaacagttg ctgagcgcat tagagcagct acagcaggac tactcgaaaa ggctggacga
     1561 gtgggagaac gccttcgcgg aatggcggac gatgtctggg cttatgcaac gggagaacgc
     1621 ggcgctgagc gagcgcgtca cgggcttgag caggcaggtg cagagtttga gcgatcagct
     1681 gcgccgattg tcgcaaggct gaatggcatc gaggcgcacc gggagcagga gcgcgcggcg
     1741 cagcaccaga aagcgctgga gctggaacga tcgcagcggc agaaaggttt tgagcttgga
     1801 atgtagttaa tcaatgaaaa gggatgcgtt atgaaatgtt tctgggcttg ctcagtctcg
     1861 gtggttttgg ctatgtcatt tacagtcgct gctgcggaga ataacgacaa cgttacatat
     1921 acaaaaccgt cagccgggag ggacttatat cttacctgta aaggttatca ggtgaagggg
     1981 gcatctgtaa ttaacgtcga gaaaccttct taccttgaag gtactggtga gcaggttgaa
     2041 tttgcaatca ttactcctgt tggtgcgtct gacggactca aggtcttttt tgattcatgg
     2101 ggtttttctt catttggatt gattgaaagc catgtgatgc atgagcactg gtctttagtt
     2161 catgttgggg ggcgtgcttc agtcaaaggt gaaaagattg attattcatt tgtagataac
     2221 cagccacagg agggggggag ctataaaccc aaaacaggtg tgttcctgta cggctgcatg
     2281 aactcggata aaagaccatc ttgggtcgaa aagatctaca caagaacagg tgtgtttcga
     2341 gggggcatag aagactaaaa tactgcttac agcatgaaaa tccattgaac agagtgtcag
     2401 ttcatttccg tgcataacta tgcataccgt aagaacttac ggggggcatt tatcggcgtt
     2461 tttacgtggt aagaggtgct cttttgcggt tcatggcgca gaacggtgct gaggctgtct
     2521 gcggcggtgc gaacaaggga tgttatggta aataagagtg tcttttggat attttaaatc
     2581 tgttttttta ttaaatttaa tgggggcaga aaatggggga ttttgagcgt acctttggag
     2641 ctggtgctga ttttgatcgg ataattgatg agaataacag cgaatatttt cgagaagatc
     2701 gccgaaaaac taaaaattca aaaaaaaaat tctcttcttt tcaagatgct ttggtttggg
     2761 cgaaagctaa tcctggcaaa acgataataa gatctccaga cggtgtgggg tttattgaaa
     2821 aatgaaagag tggattcact gtaaaacaac agtcattttt tggacaacac tgtctatata
     2881 cgtactcata tgtttcacag tgtcgtatgt tggagtctat gttacgtacg ttgcagggcc
     2941 tattttgtta gttacaggca ttcttgcatg gcttacgaaa cctagctgta atccaaagtg
     3001 actgggctag acgaacttta tccccccata aaacccgctt ttttaaacgg atcttcttga
     3061 gatcattcat ggagaatcgt aatctcttag cactgtaaac gaaaaaaccg cccagcaagg
     3121 cggttttttc gaaggttctc agagcagcaa ctctttgaac cgaggtaact ggcttggagg
     3181 agcacagtca ccaaaacttg tcctttcagt ttagccttaa ccggcgcata acttcaagac
     3241 taactcctct aaatcagtta ccagtggctg ctgccagtgg cgttttgtcg tgtctttccg
     3301 ggttggactc aagtcgatag ttaccggata aggcgcagcg gtcggactga acggggggtt
     3361 cgtgcataca gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa
     3421 ttagataaac gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga
     3481 gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc tgtcgggttt
     3541 cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg
     3601 aaaacggcaa cgccgcatcc tttttccgcc ctttctgatt gatttccgct tagtctcttt
     3661 atccagccat tcgtaagctg gcaacgcccg ccgcagtctc acggcaggag cgcagcgacc
     3721 gagtgagcga ggaggcgtca tttcaccggg atctgcattt cgcacttacg ccccacagca
     3781 gcattttctc cctgccacat gaagcacttt cctgaatccc cactccgatt taccatgtta
     3841 agccagtata cactccgcta gcgctacgtg actggttcag ggctgcgccc cgaaacccgc
     3901 tgaaaagtac tgccgcgccc tgcgggcttg cccaacaccg ctccggacag gaaaaaattt
     3961 cccgcccgaa ccggtgttat ccggaggatg gtttggcaga aaagcgcaac aaaatgc
//
