LOCUS       NZ_CP058687             4742 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain MBT-5 plasmid unnamed5, complete sequence.
ACCESSION   NZ_CP058687
VERSION     NZ_CP058687.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15423745
            Assembly: GCF_014058445.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Fiedler,G., Kabisch,J., Brinks,E., Sprotte,S., Boehnlein,C. and
            Franz,C.M.A.P.
  TITLE     Complete Genome Sequence of a Shiga Toxin-Producing Escherichia coli
            O26:H11 Strain (Sequence Type 21) and Two Draft Genome Sequences of
            Listeria monocytogenes Strains (Clonal Complex 1 [CC1] and CC59)
            Isolated from Fresh Produce in Germany
  JOURNAL   Microbiol Resour Announc 9 (49), e00973-20 (2020)
   PUBMED   33272986
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Fiedler,G., Kabisch,J., Brinks,E., Sprotte,S., Boehnlein,C. and
            Franz,C.M.A.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2020) Microbiology and Biotechnology, Max
            Rubner-Institut, Hermann-Weigmann-Str. 1, Kiel 24103, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: "Other" unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 260x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/15/2023 02:13:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,121
            CDSs (total)                      :: 5,980
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 141
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 444
            CDSs (without protein)            :: 444
            Pseudo Genes (ambiguous residues) :: 0 of 444
            Pseudo Genes (frameshifted)       :: 240 of 444
            Pseudo Genes (incomplete)         :: 204 of 444
            Pseudo Genes (internal stop)      :: 75 of 444
            Pseudo Genes (multiple problems)  :: 68 of 444
            Pseudo Genes (short protein)      :: 3 of 444
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058687.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MBT-5"
                     /serotype="O26:H11"
                     /isolation_source="ready to eat mixed salad leaves
                     (obtained from discount store)"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="Germany: Kiel"
                     /lat_lon="54.19166 N 10.07240 E"
                     /collection_date="2015-07-20"
                     /collected_by="Gregor Fiedler"
     gene            complement(502..696)
                     /locus_tag="HZT35_RS30200"
                     /old_locus_tag="HZT35_30200"
     CDS             complement(502..696)
                     /locus_tag="HZT35_RS30200"
                     /old_locus_tag="HZT35_30200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041633.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1772..2728
                     /locus_tag="HZT35_RS30205"
                     /old_locus_tag="HZT35_30205"
     CDS             1772..2728
                     /locus_tag="HZT35_RS30205"
                     /old_locus_tag="HZT35_30205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000280763.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQEAGKY
                     RGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     gene            2712..3578
                     /locus_tag="HZT35_RS30210"
                     /old_locus_tag="HZT35_30210"
     CDS             2712..3578
                     /locus_tag="HZT35_RS30210"
                     /old_locus_tag="HZT35_30210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="WP_000409995.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            3575..4228
                     /locus_tag="HZT35_RS30215"
                     /old_locus_tag="HZT35_30215"
     CDS             3575..4228
                     /locus_tag="HZT35_RS30215"
                     /old_locus_tag="HZT35_30215"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /GO_function="GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein phosphatase"
                     /protein_id="WP_000560060.1"
                     /translation="MIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCSEWNMK
                     ILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYNKSGVL
                     LTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFDLNDEN
                     KEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS"
                     /name="PP2C family serine/threonine-protein phosphatase"
     Typing          347..429
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.79e-36"
     Typing          733..831
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          956..1211
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="89.0"
                     /evalue="1.21e-116"
     Typing          complement(2..334)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.433"
                     /coverage="100.0"
                     /evalue="6.65e-137"
     Typing          complement(4414..4737)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.231"
                     /coverage="100.0"
                     /evalue="1.4900000000000002e-132"
ORIGIN
        1 gggaaacgtc tggtatcttt aagtcctgtc gggtttcgcc acctctgact tgagcgtcga
       61 tttctgtgat gctcgtcagg gggggcggag cctatggaaa aacgcctgcg gcgcggcctt
      121 cttcctgttc ttggtctttt gctcacatgt tctttccggc cttatcccct gattctgtgg
      181 ataaccgtgc tgccgttttt gtgtaagtca gtaccgctcg ccgcagccga acgaccgagc
      241 gtagcgagtc agtgagcgag gaagcggaaa agcgcctgga cgcgcatttt ctccttacgc
      301 atctgtgcgg aatttcacac ccggcatggg gcacttttca tacaatctgc actgatgccg
      361 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcaggg cttcgccccg
      421 acacccgctg acgcgccctg gcgggcttgt ctgctgccga aaacgctcgc ggacaagccg
      481 tgagcgcctc cgtcagcttc gttatgttcc tgtttcactt tcggtctcaa agcgaacctg
      541 gatgctgttc tggagttctt ccgcgagttc gtgcagtcgt tcgcacatgg ccgcctgttc
      601 gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt tgataaatcg
      661 cgccatattc agcgcagttt gttgctgttt gttcaccttc cgttgttctc cgtcagtacc
      721 gggtgtcggg tggaccctga ccaggtggta attgtaagac gttgcgggcg acggtattac
      781 aattgcacat cctgtcctgt ttttttagga cgtatcatgg cacagcatat caactgataa
      841 ataatccctg tgcagtgcag ccgctgttgc ataaaatttc gctctgcgtc gagtgtttct
      901 taatcaataa ttccgctgat actccggaaa aacgcgagtc tgttctcatg cctgaaatgc
      961 ccacacccca cgcaaaaaca attttttgct gatttttctt tataaataga gagttatgac
     1021 aaattagttc ttcttgctct ctttgtgata tttaaaaaag cggtgtcggc gcggtgttgt
     1081 agctgcgcca acaccgcttt ttaggggtgg tactgactat tttcataaaa aacatcattt
     1141 tatattaggg gtgctgctag cggcgcggtg tgttttttta caggacgccc ctgggggcgc
     1201 tgctaggggt gtctgttcag atatgtagca atgagtcagg gattcacgaa ctgaaaatgt
     1261 ccgaaaatgt gtctcatggg caccgtatgc gcccaaaccg atcgccattc actttcatgc
     1321 atagctatgc agtgagctga aagcgatcct gacgcatttt tccggttttc gcttggtaaa
     1381 acatctcttt ttgcggtgtt cgcggcagaa tcgcgttcag cgcgttttag cggtgcgcgt
     1441 aatgcgacgt tatggtaaat gacgctgaaa aatgatcttt tacgctcatc gtttatgagc
     1501 aacagatgtc ctaatcatgg atttgaacta taaccgccgc tgaattgatc gcatgaggtg
     1561 ttatgaaaac ggtgatataa gtaaactttt tcttgacttc ctccttatta tcctataaaa
     1621 taaatcctta ggatttattt tataggatat caaaaatata aaaatggcaa atgcttgttt
     1681 gttattattt tttccttgac aatcaatctc taatagaata tacgtcctta ggacgtatat
     1741 tctattagag actaaaccga aaaggttgaa tatgtatctt atcgaagaag cgctaacgtc
     1801 ttggttgttg aatgaatata aaaaaccagt cataaccaaa gaagaaatct ctttgcagat
     1861 ttaccttttt tatttaaaaa aagaatataa tggtgagagg atatatcaaa ttagaaagaa
     1921 caaagcagga cctaatgaat ttgaaaaaaa tatattaaaa ttattaaagt caggggtttt
     1981 atcaagagtt gaaagtaacg ataagcatca tgcttacccc catttaattg gcggtttcta
     2041 tgaggtcttt tttgttaaag gtaacgatgg ttctccggaa aatgatatag tgtgctctac
     2101 gtatccatat ggatacttat cctatttatc cgctatggat tggcataaca tcacagataa
     2161 aatacccaag ataatccact ttacgacatg tcgttccgaa cagtggaaga ataagttcta
     2221 tcatgatttt caaaaggata atcctgatat agacgaagcc aaacaattca tgcctagatt
     2281 tccaagaaat tctgaggtca atggaataaa aattatcgtt acacaagaaa caaattatgt
     2341 tgagccaatg aaggctcgta attcccctgt cagggtatca tctatcggta agacctttat
     2401 ggatatgacc aggcggcctg attactgtgg tggtgaggag catgtattgg aatcatttat
     2461 tgagcacggt aaaaaatatg cacctctgat tataaaggaa gtcgataagc ttggccgtca
     2521 tattgacaag gtaagggttg gattcatttt ggatagaatt attggggtta ataataaaaa
     2581 aataagcgaa tggaaacaag aggcgggtaa atacagaggt agttcgaaag tgctatctcc
     2641 aaagagtcca ttctcacctt actttgatga agaatggttt atttctatta atgttgatat
     2701 ggttaagtct tatggaaaaa gaaattaata attggatcga agagtctggt gggaagtaca
     2761 ggttgatgcg gcaggctgta catataatac ttaaatctat ttctatagag gagagtttga
     2821 atgaaatgat ggttatgaag ggagggactc ttcttggtgt taagcatggt agtgatagat
     2881 acacaaagga tattgatttt tctaccgaaa aaaaactttc tgaaattgac ttgggggatt
     2941 ttttcaatag gtttaatgac tcaatggatg ttgctgaggc tgaattggga tatggtatta
     3001 aatgtaaagt tcaatctcat aaaattcaac ctaatgcaca aggaacattt ccaactgtac
     3061 agataaatat agccttttgc gatcgttcta atgcgtctgc aatgaaaagg cttgaatcta
     3121 atcaatcacc ttcagtgata aatatcgatt ttagctttaa cgaaaaaacg tatgattttg
     3181 actttttatc attagatggt gatgatgaca atgatagcgt aaagacctac agcttaactg
     3241 atttgatggc tgaaaaatat cgatccgttt tgcaacaaaa agttagagag agaaatcgag
     3301 agcaagatat atatgatatc aattatcttt taggtaaata tgagttttct cggcaagata
     3361 agtataaaat actggagtct cttttaaaaa agagtgaagg taaacaattg gataatctct
     3421 taaacgttaa aggtataagg gatgtagaaa ttatcgagcg ttccagtcag cgatatcagg
     3481 atttatcatc caccgttaaa tcattacctc tttttgaaga tgcttatgaa aaagttgcct
     3541 gtttctatga aagtcttccg tgggatatat tcaaatgata gcgttcagtt ttagtgatat
     3601 tggtaacaga tgtgaaaact tagacttatt atctacgata aattgtcaaa atgatgtgtt
     3661 tggaatggtt attatggatg ggtattcctg ctctgagtgg aatatgaaaa ttctctctgg
     3721 taggttagat aagtgcgtgt tttctggtga tctgaataaa gatattattg aaccatgcaa
     3781 gtcagttcct gttaaggctg cagtatgttt cctttttaaa aataatacgc acctccatat
     3841 atataacgca ggagattgcc gtgtatataa caaaagtgga gtccttctaa cagaggataa
     3901 ctcggttgcc tggaaaagat taaaggaaaa tggaatcgat aataaaaaaa tacctcacct
     3961 cgtttgttat tgcccagaca gaaattatct aacatcattt gttgatttct gctctgatga
     4021 gataattttc aatcattcag tttttgattt aaatgatgaa aacaaagagg ttattttatg
     4081 tactgatggg ttttgggagc attttaatga tggtactgtt attgacctca tgaaagataa
     4141 cccagaatct aacttggcta aatttactaa aaatatcccg ggtaacgcag agaatcatac
     4201 ggtttgctac gttagattcc cttcttgatt tcgtgttttt tgaagaagct attgagatct
     4261 tcgtgtcata tagataatct cttttctgta aaagaaaaaa accgcctttg cggggcggtt
     4321 ttctcttagg ttcaaagagt ttgcggctcg aagaaccaag gtaactggct tggaggagcg
     4381 cagtcaccaa aacctgttct ttcagtttag ccttaactgg cgcatgactt caagactaac
     4441 tcctctaaat cagttaccag tggctgctgc cagtggcgtt aaggcgtgtc ttaccgggtt
     4501 ggactcaaga cgatagttac cggataaggc gcagcagtcg ggctgaacgg ggggttcgtg
     4561 cacacagccc agcttggagc gaacgaccta caccgaactg agataccaac agcgtgagct
     4621 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4681 ggtcggaaca ggagagcgca cgagggagcc accaggggga aacgtctggt atctttacga
     4741 gg
//
