LOCUS       NZ_CP058686             6715 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain MBT-5 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP058686
VERSION     NZ_CP058686.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15423745
            Assembly: GCF_014058445.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Fiedler,G., Kabisch,J., Brinks,E., Sprotte,S., Boehnlein,C. and
            Franz,C.M.A.P.
  TITLE     Complete Genome Sequence of a Shiga Toxin-Producing Escherichia coli
            O26:H11 Strain (Sequence Type 21) and Two Draft Genome Sequences of
            Listeria monocytogenes Strains (Clonal Complex 1 [CC1] and CC59)
            Isolated from Fresh Produce in Germany
  JOURNAL   Microbiol Resour Announc 9 (49), e00973-20 (2020)
   PUBMED   33272986
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Fiedler,G., Kabisch,J., Brinks,E., Sprotte,S., Boehnlein,C. and
            Franz,C.M.A.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2020) Microbiology and Biotechnology, Max
            Rubner-Institut, Hermann-Weigmann-Str. 1, Kiel 24103, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: "Other" unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 260x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/15/2023 02:13:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,121
            CDSs (total)                      :: 5,980
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 141
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 444
            CDSs (without protein)            :: 444
            Pseudo Genes (ambiguous residues) :: 0 of 444
            Pseudo Genes (frameshifted)       :: 240 of 444
            Pseudo Genes (incomplete)         :: 204 of 444
            Pseudo Genes (internal stop)      :: 75 of 444
            Pseudo Genes (multiple problems)  :: 68 of 444
            Pseudo Genes (short protein)      :: 3 of 444
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058686.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MBT-5"
                     /serotype="O26:H11"
                     /isolation_source="ready to eat mixed salad leaves
                     (obtained from discount store)"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="Germany: Kiel"
                     /lat_lon="54.19166 N 10.07240 E"
                     /collection_date="2015-07-20"
                     /collected_by="Gregor Fiedler"
     gene            join(6689..6715,1..132)
                     /locus_tag="HZT35_RS30875"
     CDS             join(6689..6715,1..132)
                     /locus_tag="HZT35_RS30875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001557254.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDLTPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="hypothetical protein"
     gene            complement(360..1517)
                     /locus_tag="HZT35_RS30155"
                     /old_locus_tag="HZT35_30155"
     CDS             complement(360..1517)
                     /locus_tag="HZT35_RS30155"
                     /old_locus_tag="HZT35_30155"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:1904047 -
                     S-adenosyl-L-methionine binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001219937.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSHP
                     IKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKRIK
                     PKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGKLDV
                     RDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTVRGVN
                     RPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGNAVPVE
                     LAKYVAVTIMEYEKKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDSEG
                     VDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
                     /name="DNA cytosine methyltransferase"
     gene            1605..2666
                     /locus_tag="HZT35_RS30160"
                     /old_locus_tag="HZT35_30160"
     CDS             1605..2666
                     /locus_tag="HZT35_RS30160"
                     /old_locus_tag="HZT35_30160"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="WP_044862115.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMNP
                     IYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVIKG
                     NNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHEIRK
                     IFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKSPILD
                     QNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNINRIYT
                     EMNFGYQSDKEFSLNGKITKAYMVHPRRTKPILPPSVLKEIILNGGQNKLSPERRFDQS
                     VYYTASHLFDEIK"
                     /name="HindVP family restriction endonuclease"
     gene            3057..3440
                     /locus_tag="HZT35_RS30165"
                     /old_locus_tag="HZT35_30165"
     CDS             3057..3440
                     /locus_tag="HZT35_RS30165"
                     /old_locus_tag="HZT35_30165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000065853.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKELI
                     SKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKIVFTLGVVNKLKIEDGWINHKI
                     SDTEDAVLSVGKIINLIEDSQHG"
                     /name="hypothetical protein"
     gene            3433..3639
                     /locus_tag="HZT35_RS30170"
                     /old_locus_tag="HZT35_30170"
     CDS             3433..3639
                     /locus_tag="HZT35_RS30170"
                     /old_locus_tag="HZT35_30170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096539.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQS
                     GDGKLMATYRRNKKAIDKLDAEG"
                     /name="hypothetical protein"
     gene            complement(4014..4247)
                     /locus_tag="HZT35_RS30175"
                     /old_locus_tag="HZT35_30175"
     CDS             complement(4014..4247)
                     /locus_tag="HZT35_RS30175"
                     /old_locus_tag="HZT35_30175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001448503.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4254..4772)
                     /locus_tag="HZT35_RS30180"
                     /old_locus_tag="HZT35_30180"
     CDS             complement(4254..4772)
                     /locus_tag="HZT35_RS30180"
                     /old_locus_tag="HZT35_30180"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001447511.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGMSAEVTHYQ
                     GKDWIQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4810..5241)
                     /locus_tag="HZT35_RS30880"
                     /pseudo=""
     CDS             complement(<4810..5241)
                     /locus_tag="HZT35_RS30880"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5450..5773)
                     /locus_tag="HZT35_RS30190"
                     /old_locus_tag="HZT35_30190"
     CDS             complement(5450..5773)
                     /locus_tag="HZT35_RS30190"
                     /old_locus_tag="HZT35_30190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955994.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIEGELRSLRQVVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5838..6029
                     /locus_tag="HZT35_RS30195"
                     /old_locus_tag="HZT35_30195"
     CDS             5838..6029
                     /locus_tag="HZT35_RS30195"
                     /old_locus_tag="HZT35_30195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165984.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGEDL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(6108..6192)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          6212..6715
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.069"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3917..5460)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.529"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(5457..5806)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.162"
                     /coverage="99.0"
                     /evalue="1.96e-64"
ORIGIN
        1 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca cgacttaacg
       61 ccactggcag cagccactgg taactggaca aggtggattg agatattaag agttcttgaa
      121 gtggtggcct aactgcggct acactagaag gacagtattt ggtatctgcg ctccaccaag
      181 ccagttacct tcggaagaaa gagttggtag ctctagattc cggcaaacaa accaccgttg
      241 gtagcggtgg tttttttgtt tacaggcagc agattacgcg cagaaaaaaa ggatcacaag
      301 aggatccttt gatcttttta tcccctcccc ctcccctgag agacataaat tcgtatttat
      361 taccttcgac aatattcgta atataattta aaagcccttc ttaactgaga cttcaggcta
      421 accccaagcc tgacaaacga ttcaagcctt tccagtttca taataatttc atctatttca
      481 cagttataaa aatccacccc ctctgaatca aaaaatgaaa cacctctgca acatctagat
      541 ataatatcgc tagaggttct ttttgttagt ttcttttcat tcagcaacca cgccctaaag
      601 ccttctttat cataaatacc ttttacttgt tttttttcat actccatgat tgtcaccgca
      661 acatattttg ctaactcaac cggaacagca ttacctatca tttgctccaa atttgttttt
      721 gcacctttaa atttaaagtc ttcaggaaat gtttgcaatc ttgctctttc aaaggttgtt
      781 aatggcctta cattctcaga aatactgaca ggatcgcctg catgccctaa atatccatca
      841 ggtatcggtc tattaacccc tcttactgtt ggcgcaggtt catcaataga gaaaatagcc
      901 cttctgttat aatttctcgg atgtctgtaa taatactcaa tccccaatga atcaccaaga
      961 taatttctaa ctgtcatact atctttcgat atcccgcaca tcacttcgtt aaggataaaa
     1021 ccatccctca cgtcaagctt tccaattaca aataatcttt ttcttttttg cggaacacca
     1081 cacttactgg catccaaaac aatttctgtt aacccatacc ctgattcttt aaagattcct
     1141 cgagcttcta aataggcacc acttcttaat gcccgatcta cattttccat cacaaaccac
     1201 tttggcttta ttctttttat aatttctgaa aatgagcgag ttaagttagc tctagcacct
     1261 tcaattctta aacctgcatg actgaagtct tggcaaggag gccctccaat aatcatatcg
     1321 aagtctatat ctttaagctc cctcaccaca tcatcaacat tgctcaaatc tagttttttt
     1381 atcggatggc taaaattatc tctgtaacat tcaatagcag gatcccagaa atcataagca
     1441 cctacaacat caaacccccc cttcaaaaag cctagggaca accccccaca cccactaaaa
     1501 agatcaatta ttctcataac ataaaaaact cattatcata tcactccaca tcatgatata
     1561 gaaacacacc atagacgtca ataaattgac gtcaggagca aaaagtgagc aaaatagaat
     1621 ctctttatgg tttaaaaaac accaacagga aagagagcga tctgtgggga aagaaccaat
     1681 ttaacagttc ttttcccacc tctctagcat gttatatgag agacaacaaa atgaatccga
     1741 tttatcttta tttagataag gatttaaaag tgcatgccaa agaagtatct tttgacttcg
     1801 tttttaacac aacattaaaa aatagtgatc ttgaatttca atttgagaaa aaatatgaac
     1861 cataccaaca gtatgcttat gatgacataa aaggaataga tctagttata aaaggaaaca
     1921 atagtttttt aaaagcacta gaaataaaat taaccgtact tccagatagc acgacatata
     1981 aagaaaacga gagcgaatgg agttcagaga ttgtaataag acctgcaagc acttcttatt
     2041 gtgcccttgg cataatgcac tcttgcagcg ataaattcca tgaaataaga aaaatatttg
     2101 aatccacatg cgaaagcata cagcactggg ggaatgaaac tgaagttctt tcaaagaaag
     2161 aaaatatatt aaattgccta aacactttcc aagaaaattt tcttgattac cagaaacctt
     2221 ttttaatgca accattatgg aaaacaaaag gcaagagccc aatattagat caaaatgcct
     2281 ttgacatatt catctggagt gattttgctt tgtgtcgaac ctttattgat agatcctcat
     2341 ataacaattc atctgccagc agactaatga gatcatcagc cagactcgcc agaatggtgt
     2401 atgaaattgc gagcaaaaac aagacaaaca tcaatagaat ttatactgag atgaactttg
     2461 gatatcaaag tgataaagag ttctcgctaa acggaaaaat aacaaaagct tatatggttc
     2521 acccaagaag aacaaaaccc atcctcccac catcagtatt aaaagaaata attttaaatg
     2581 gcgggcaaaa taaattaagc ccagagagac gctttgatca aagcgtttat tatactgcat
     2641 ctcacctgtt tgatgaaata aagtaatttt tcttggtggc tcgccagccc tgaattcgcc
     2701 catcaaaaat cagtagcgga acaggcatac tagacatgct cgcttttacg gcgagcatct
     2761 tgacggagtt tgagacgcaa tggaacagca accaactcta tgcatcacaa gctcaaaaat
     2821 gattaccatg cattgaactc tagttacctt tgccaatgat tgctcctggt tgcttcatgc
     2881 ccgtaaaggc aatttcgacg gtcattacat tgaacataac atctcgaccg cgtactgaac
     2941 ggtctcagcc ggatcttcga acccatctat cgtagaattg atactctacc tctgtgacta
     3001 acaagacagt tccgctactc ttctgagtat tcattcatca aaaagcagga tatccagtgt
     3061 caaacattgt tcatctatct gataaaaaac aaaaaaacaa accagttgat atttcttttg
     3121 atgattacac agacaatctc agcaaagggt tcgtcaaaaa tctcagcaat aaacaaaaaa
     3181 aagaattaat tagtaaaatc aaagcgatac ttaaatcgag gaatgtagcg ttccgttcga
     3241 cgccgccctc tgattggaaa gagaaaagta gaacctttgc aattttcttt cctgaaaaaa
     3301 agattgtttt tacattagga gtagtgaaca aattaaagat agaggacggt tggataaatc
     3361 ataaaatcag cgacacagaa gatgccgtct tatctgttgg aaaaataatc aaccttatag
     3421 aggattcaca gcatggctaa attacctcaa gaaaagccga aagaagtcat ccaagccgga
     3481 tttgatgatg ttaaaaaggc ctataaaaaa ggcgctaaga ctgtaaacga tacctatgct
     3541 gagcaagcag gtcgtgactt acaatcaggc gatggtaaac taatggctac ctatcggcgc
     3601 aataaaaaag cgatagacaa gttagatgct gaaggttgat ctgaaaagtc agtcttctgg
     3661 gcggactttt cgtctctaag actggctgca tattgtattt accataacat ccgttacgca
     3721 caccgctaaa acgcgttcgg tgcgatcacg gcagcagaca ggtaaaaatg gcaacaaacc
     3781 acccgaaaaa ctgccgcgat cgcgcctgat aaattttaac cgcatgaata cctatgcaac
     3841 cagatggtgc agactacatt agtccgtcaa aatccactga acctgccatt ttttatagtt
     3901 tcgccatcac agcgacaacg aaggaccatc cagtatgcgt tcctgtattt ccggctgacg
     3961 ctcccgttcc agggataacg catgttcgcg ctcctgtatc agccgttcct ctcttatctc
     4021 cagttctcgc tgtataactg gctcaagcgt tctgtccgct cgctcaagtg ctgcacctgc
     4081 tgactcaact gcatgacccg ctcgttcagc atcgcgttgt cccgttgcgt aagcgaaaac
     4141 atcttctgca attccacgaa ggcgctctcc cattcgttca gccgctgcat atagtcctgt
     4201 tgcagctgct ctaaggcgtt cagcaaatgt gtttccagct ctgtcattcc gtatcactcc
     4261 gtcagttgta tccagtcctt cccctgatag tgcgtcacct ctgctgacat cccttgcgga
     4321 atgaccagaa atttccttct cccgtcctgc acaaactcca cgccccatgt cttcgcgttc
     4381 agtttctgca atgtctcttc ctgcttcctg atttcttcca ggttcgcctg tatcctcccg
     4441 ccaagatacc agagcgtccc gccactcgcg gtaaacagga gaaagactat ccccagtaac
     4501 atcatgcccg tattccctgc cagctttaac acgtccttcc tgtgctgcat catcgcctct
     4561 ttcacccctt ccctgtgttt ttccagcgat ttctctgtcg aggctgtgaa cagggctata
     4621 gcgtctctga ttttcgtctc gtttgaggtc acagcctcgc tgacagactc gccgagcttc
     4681 ctgaactcgt cgttcagcat tttctctgta gattcggctc tctctttcag ctttttctcg
     4741 aactccgcgc ccgtctgtaa aagattgctc ataaaacgct cctttcagcc tgatattctt
     4801 cccgccgttc gggtctgcaa tgctgatgct gcttcgcgtc accctgacca cttcaagccc
     4861 cgcctctgtg agcgcctgaa tcacatcctg acggcctttt atctccccta catgataaag
     4921 agcgtctatc ccgcgtgtga cgctctcagc aagcgcctgt ttcgtttccg gcaggttatc
     4981 agagagagtc agtatccggc ggttctccgg ggcgttgggg tcatgcagcc cgtaatggtg
     5041 attcaccagt gtctgccagg catcaattct cggcctgtct gctcggtcgt agtacggctg
     5101 gaggcgtttt ccggtctgta gctccatgtt cggaatgaca aaattcagct caagacgccc
     5161 cttgtcctgg tgctccaccc acagaatgct gtactgattt ttttcgagac cgggcatcag
     5221 tacgcgctca aagctctcca tcactttttc acgtcctccc ggcggcagtt ccttctccgc
     5281 gaacgacaga acaccggacg tgtatttctt cgcaaatggc gtggcatcga tgagttccct
     5341 gacttcttcc ggattaccct gaagcaccgt tgccccttcc cggttacgct cccttcccag
     5401 caggtaatca accggaccac tgccaccgcc ttttcccctg gcatgaaatt taacaatcat
     5461 cccgcgctcc ctgctccctg acgacctgcc gtaagctgcg caactctccc tcgatggcca
     5521 tcagtgcggc caccacctga acccggtcac cggaagacca ctgcccgctg tttaccttcc
     5581 tcgctgtctg attcaggtta ttcccgatgg cggccagctg acgcagtaac ggcggtgcca
     5641 gtgtcggcag cttcccggaa cgggcaaccg gctcacccag gcagacccgc cgcatccata
     5701 ccgccagttg tttaccctca cagcgttcca gtaaccgggc atgttcatca tcagtaaccc
     5761 gtatcgtgag catcctctcg cgtttcatcg gtatcattac ccccatgaac agaaatcccc
     5821 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     5881 tcagaagcca gacattaacg ctgctggaga aactcaacga actggacgca gatgaacagg
     5941 ccgatatttg tgaatctctt cacgaccacg ccgatgagct ttaccgcagc tgcctcgcgc
     6001 gtttcggtga tgacggtgaa gacctctgac acatgcagct cccggagacg gtcacagctt
     6061 gcctgtgagc ggatgccggg agcagacaag cccgtcaggg cgcgtcaggg ggtttcgggg
     6121 cgaagccctg aaccagtcac gtagcgatag cggagtgtat actggcttaa ctatgtggca
     6181 tcagtgcgga ttataagaaa agagcaccat atgcggtgtg aaataccgca cagatgcgta
     6241 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctacgctcg
     6301 gtcgttcggc tgcggcgagc ggagtcggct cactcaaagg cggtaatacg gttatccaca
     6361 gaatcagggg ataaggccgg aaagaacatg tgagcaaaaa ggcaaaggac aacaaaaggc
     6421 cgcgccgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca gaaatcgacg
     6481 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6541 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6601 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgttggtatc tcagttcggt
     6661 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgac
//
