LOCUS       NZ_CP058660             2101 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain WCHEC035125 plasmid p3_035125, complete
            sequence.
ACCESSION   NZ_CP058660
VERSION     NZ_CP058660.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09202771
            Assembly: GCF_013426095.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,K., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain 035125
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Ma,K., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUL-2020) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 04:03:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,864
            CDSs (total)                      :: 4,739
            Genes (coding)                    :: 4,505
            CDSs (with protein)               :: 4,505
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 67 of 234
            Pseudo Genes (incomplete)         :: 156 of 234
            Pseudo Genes (internal stop)      :: 43 of 234
            Pseudo Genes (multiple problems)  :: 32 of 234
            Pseudo Genes (short protein)      :: 4 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058660.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WCHEC035125"
                     /isolation_source="sewage"
                     /db_xref="taxon:562"
                     /plasmid="p3_035125"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2018"
     gene            complement(join(1156..2101,1..20))
                     /locus_tag="HZR25_RS24030"
                     /old_locus_tag="HZR25_24040"
     CDS             complement(join(1156..2101,1..20))
                     /locus_tag="HZR25_RS24030"
                     /old_locus_tag="HZR25_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            complement(886..1152)
                     /locus_tag="HZR25_RS24035"
                     /old_locus_tag="HZR25_24045"
     CDS             complement(886..1152)
                     /locus_tag="HZR25_RS24035"
                     /old_locus_tag="HZR25_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          complement(1497..1728)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 aggaatttat cttctgacat taagcctgct ccgatctggg caggctgtga tttgatgtgg
       61 tatattcatc acgtacctgt gcattgcgag ccagtacatg tcaccagagt ttgccgctga
      121 ggtgacacat tcgaaatgtt ggggagtgtt gacgcactcc ccaacgccga ttaaaaacgc
      181 ctcaattctg ctccattttc tttgacctgt caaaacccac tcaaagcctc gtcattactg
      241 ggctaaacca ctatttcata tccgccattt atcccttaag tataaataca aggatcacgc
      301 gcgagaattg cgcaaaaaat aaccacaatt ttaacccccc aaaacaagca cccccgaacg
      361 gcttacccca accttttcca cacacccgat caccaaaaca gggaataaaa acaaaaccct
      421 gtcttacagg tggatggcct cacttcgtga aaccatccac cccgcataag ttttttaatc
      481 ggtggtgtcg tcaatcacta aatcggaacc ccaaagggga cccccacgat ttagcatttg
      541 acgagattaa ctctcggaga tggcatcatg cttttaacgg actttaaaaa acggtgaacg
      601 gatgcggatt ttaatccgtg tctgtgaggc gttgagcaac gagcaaagcg aggcggtaga
      661 aagagagcca gcggcaggct caggacgata atccggggaa aatgtaccac tcgcagagtg
      721 ttaacatttg acatcggatt gtctatcctg cattggtgtc gagttggcaa ggctgaggat
      781 aatgctgagg ataatgctga ggataatgca ggatagacag agcactagag attgcatgca
      841 atctcgtacc tatcacctca gcctgccgct ggctcttttc cagaactaag aatgcgttct
      901 taaaaatcca aacttgatac cgtttaaatc tattttcaca aaaagagttt ttaccctctc
      961 gtttgttttc tgaatatata aaattaattc atcctcaaaa ccttcgtgcg gatgaaaacc
     1021 aaatgtgcca aaacttaatc catcaataag caaaataagg tttttaaacc tgtcaggaat
     1081 caccgcttca cttcttaaaa aaaataaacc aatagtctta tttccattca ggtattcaat
     1141 attaatattc atacgttaat ccgatttatc cttctcaggg gcgcgtttgt aacgccgttt
     1201 acctgaatcc cagacaaacg ccgtccgctt cccgtcatca gtgccatccc ctacatcgtc
     1261 ggcaatgaca agatcctcat tggtttctcg atccaactgg aggacgtttt ttagcgcccc
     1321 accggtcgag ataaaacggc gcttgtgaag ctgccgcgtc agctcaagaa accactcagg
     1381 atcgtttgcc atatcttccg gtttaacgga gtatttcagc gtttcagcaa ccgcgctttg
     1441 cagttgctcg gcaacgttgg ccacaacctc acctgtctta gtttttactg cccgaatatc
     1501 gatattcggc tcatagttca cccgcaagca atcgcgccag agttctaccc aacgttcgtg
     1561 cttaacgtag ttcttccctt taaaccaaga aggttgcacc atcagcagac agtgaaaatg
     1621 cggatgtgcg ctgccatcct tacctcgcgt cacctccgta gccctgatcc acccctgaac
     1681 aggtgatagc tcctttcgct tttccattcg cttaaacgcc gcattcattg ctgtaaggac
     1741 tgttccaagt tcacctatct cgcagttcct gacagttaac gtcagaaaca accatcggga
     1801 agacgggtaa tccacaacga ttttcggtag agcctgataa aaacgggctt gccacatgag
     1861 ggttcttctc cactggcaga cagggcaatg acggacacgg caaaattgcg cactacgaag
     1921 ccgtaggcgc gtttctccgg tttccatgat ggtagaccaa ccaaaacgta acaaaccgct
     1981 acaggaggcc atacggagtg catagcgctc gaattcagca gcagtctggt agataccccc
     2041 gacagaatcg gtaagcgtgc gctgggtatc ccaaactgca tcacgggggc tgtagtccga
     2101 a
//
