LOCUS       NZ_CP058655             2101 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain WCHEC035123 plasmid p3_035123, complete
            sequence.
ACCESSION   NZ_CP058655
VERSION     NZ_CP058655.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08932748
            Assembly: GCF_013426075.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,K., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain 035123
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Ma,K., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUL-2020) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 03:45:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,895
            CDSs (total)                      :: 4,770
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 73 of 239
            Pseudo Genes (incomplete)         :: 157 of 239
            Pseudo Genes (internal stop)      :: 47 of 239
            Pseudo Genes (multiple problems)  :: 37 of 239
            Pseudo Genes (short protein)      :: 4 of 239
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058655.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WCHEC035123"
                     /isolation_source="Sewage"
                     /db_xref="taxon:562"
                     /plasmid="p3_035123"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2018"
     gene            join(1826..2101,1..690)
                     /locus_tag="HZR24_RS24190"
                     /old_locus_tag="HZR24_24190"
     CDS             join(1826..2101,1..690)
                     /locus_tag="HZR24_RS24190"
                     /old_locus_tag="HZR24_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            694..960
                     /locus_tag="HZR24_RS24195"
                     /old_locus_tag="HZR24_24195"
     CDS             694..960
                     /locus_tag="HZR24_RS24195"
                     /old_locus_tag="HZR24_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          119..350
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 cgtttttatc aggctctacc gaaaatcgtt gtggattacc cgtcttcccg atggttgttt
       61 ctgacgttaa ctgtcaggaa ctgcgagata ggtgaacttg gaacagtcct tacagcaatg
      121 aatgcggcgt ttaagcgaat ggaaaagcga aaggagctat cacctgttca ggggtggatc
      181 agggctacgg aggtgacgcg aggtaaggat ggcagcgcac atccgcattt tcactgtctg
      241 ctgatggtgc aaccttcttg gtttaaaggg aagaactacg ttaagcacga acgttgggta
      301 gaactctggc gcgattgctt gcgggtgaac tatgagccga atatcgatat tcgggcagta
      361 aaaactaaga caggtgaggt tgtggccaac gttgccgagc aactgcaaag cgcggttgct
      421 gaaacgctga aatactccgt taaaccggaa gatatggcaa acgatcctga gtggtttctt
      481 gagctgacgc ggcagcttca caagcgccgt tttatctcga ccggtggggc gctaaaaaac
      541 gtcctccagt tggatcgaga aaccaatgag gatcttgtca ttgccgacga tgtaggggat
      601 ggcactgatg acgggaagcg gacggcgttt gtctgggatt caggtaaacg gcgttacaaa
      661 cgcgcccctg agaaggataa atcggattaa cgtatgaata ttaatattga atacctgaat
      721 ggaaataaga ctattggttt atttttttta agaagtgaag cggtgattcc tgacaggttt
      781 aaaaacctta ttttgcttat tgatggatta agttttggca catttggttt tcatccgcac
      841 gaaggttttg aggatgaatt aattttatat attcagaaaa caaacgagag ggtaaaaact
      901 ctttttgtga aaatagattt aaacggtatc aagtttggat ttttaagaac gcattcttag
      961 ttctggaaaa gagccagcgg caggctgagg tgataggtac gagattgcat gcaatctcta
     1021 gtgctctgtc tatcctgcat tatcctcagc attatcctca gcattatcct cagccttgcc
     1081 aactcgacac caatgcagga tagacaatcc gatgtcaaat gttaacactc tgcgagtggt
     1141 acattttccc cggattatcg tcctgagcct gccgctggct ctctttctac cgcctcgctt
     1201 tgctcgttgc tcaacgcctc acagacacgg attaaaatcc gcatccgttc accgtttttt
     1261 aaagtccgtt aaaagcatga tgccatctcc gagagttaat ctcgtcaaat gctaaatcgt
     1321 gggggtcccc tttggggttc cgatttagtg attgacgaca ccaccgatta aaaaacttat
     1381 gcggggtgga tggtttcacg aagtgaggcc atccacctgt aagacagggt tttgttttta
     1441 ttccctgttt tggtgatcgg gtgtgtggaa aaggttgggg taagccgttc gggggtgctt
     1501 gttttggggg gttaaaattg tggttatttt ttgcgcaatt ctcgcgcgtg atccttgtat
     1561 ttatacttaa gggataaatg gcggatatga aatagtggtt tagcccagta atgacgaggc
     1621 tttgagtggg ttttgacagg tcaaagaaaa tggagcagaa ttgaggcgtt tttaatcggc
     1681 gttggggagt gcgtcaacac tccccaacat ttcgaatgtg tcacctcagc ggcaaactct
     1741 ggtgacatgt actggctcgc aatgcacagg tacgtgatga atataccaca tcaaatcaca
     1801 gcctgcccag atcggagcag gcttaatgtc agaagataaa ttcctttcgg actacagccc
     1861 ccgtgatgca gtttgggata cccagcgcac gcttaccgat tctgtcgggg gtatctacca
     1921 gactgctgct gaattcgagc gctatgcact ccgtatggcc tcctgtagcg gtttgttacg
     1981 ttttggttgg tctaccatca tggaaaccgg agaaacgcgc ctacggcttc gtagtgcgca
     2041 attttgccgt gtccgtcatt gccctgtctg ccagtggaga agaaccctca tgtggcaagc
     2101 c
//
