LOCUS       NZ_CP058623             1551 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain JS316 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP058623
VERSION     NZ_CP058623.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15455438
            Assembly: GCF_014826015.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Sommer,J., Krause,F.F., Gerbracht,K.M., Wild,F., Tietgen,M.,
            Riedel-Christ,S., Sattler,J., Hamprecht,A., Kemp,V.A.J. and
            Goetting,S.
  TITLE     Sequencing of carbapenem resistant Enterobacterales
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Sommer,J., Krause,F.F., Gerbracht,K.M., Wild,F., Tietgen,M.,
            Riedel-Christ,S., Sattler,J., Hamprecht,A., Kemp,V.A.J. and
            Goetting,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2020) Institute for Medical Microbiology and
            Infection Control, University Hospital Frankfurt, Paul-Ehrlich-Str
            40, Frankfurt 60596, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/15/2023 02:14:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,726
            Genes (coding)                    :: 4,492
            CDSs (with protein)               :: 4,492
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 64 of 234
            Pseudo Genes (incomplete)         :: 154 of 234
            Pseudo Genes (internal stop)      :: 42 of 234
            Pseudo Genes (multiple problems)  :: 27 of 234
            Pseudo Genes (short protein)      :: 4 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058623.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="JS316"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="Germany"
                     /lat_lon="50.1239 N 8.5763 E"
                     /collection_date="2019-12"
                     /collected_by="Institute for Medical Microbiology and
                     Infection Control, University Hospital Frankfurt"
     gene            1..477
                     /locus_tag="HZF20_RS23980"
                     /old_locus_tag="HZF20_23940"
     CDS             1..477
                     /locus_tag="HZF20_RS23980"
                     /old_locus_tag="HZF20_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     gene            525..815
                     /locus_tag="HZF20_RS23985"
                     /old_locus_tag="HZF20_23945"
     CDS             525..815
                     /locus_tag="HZF20_RS23985"
                     /old_locus_tag="HZF20_23945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_153274583.1"
                     /translation="MIVYPALLFCRLKILCVFLLPVLQKKPSRHGSGSALSATGNRLGR
                     TPDPHRCNTDGLRPPTNRVATCSVLMGFAGGWRGSWRPESPWLGVSARLTS"
                     /name="hypothetical protein"
     Typing          1070..1155
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.552"
                     /coverage="95.0"
                     /evalue="9.38e-33"
     Typing          complement(1365..1473)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.091"
                     /coverage="100.0"
                     /evalue="9.09e-49"
     Typing          complement(709..969)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.748"
                     /coverage="100.0"
                     /evalue="8.56e-103"
ORIGIN
        1 atgtttattg attcagaaaa acgactgaaa caactttcag atgaggcaaa gaaaaacacc
       61 gaggatctcg aagaagcaaa gaaaaattca aggtttacac aggtatcccc aaaaggttgg
      121 gaacgcgttc gagagctgct gaaggatagc caaggcatat cagcactgaa gctgtactca
      181 tttttagcgg agcatatcga tcctacgtgt ggcgctgtcg ttgcggatca gcaattccta
      241 gctgaaaaac ttggagttag cagaagcaca attattcgtt ggctcaatta cttagaatca
      301 aaaaatgcat tagttagaat ccccgttgct ggtaaggttt gtgcgtatgc cctcgatcca
      361 catgaagtct ggaagggata caacactacg aaaaaccatg cagcgtttgt cactaaaaca
      421 ctggtcaaca aagacggtga tattcagcgc cgaatcatgg ccatgttttc aaattgagct
      481 agcggcaggc ggacaatcag gggctacgtg ttaacgttct gaccatgatt gtctatcctg
      541 cattgctctt ttgccgcctc aaaatccttt gcgtgttttt gctccccgtt ctccagaaaa
      601 aaccgagccg ccacggttcc ggcagcgcct tgagcgctac cggaaaccgt ctggggagaa
      661 ccccagaccc ccatcgctgt aacaccgacg gtctacgacc acctaccaac cgtgtggcta
      721 cctgttccgt gctaatgggc tttgcagggg gctggcgcgg ttcctggcga cctgaaagcc
      781 cctggttagg ggtatcggcg cgcctgacct cctgaaaccc tctgtaagcg tttttcgcgc
      841 cttccatgcc ctggcgggca tctaaaaccc gtttcgtgcg tctgagagct tgtgagcgcg
      901 tttgaggggc tatctggcga ctcatccccg aaagaacaac aacgggatca ccgacaacat
      961 gcgagaggtg atcccgctcg ccgcaagtcg atgaccgagc gtagcgagcg aatcgacgag
     1021 gaagcggaag agaaccggaa accacattga gcacgtacgc actgatgcgg ggtgtcgggg
     1081 cgaagccctg accagatggc aattgtgata gcgtcgcgtg tgacggtatt acaattacac
     1141 atcctgtccc gatttctgag gcgttttaac ggtgaacgga cggcaaaaac atgatggagc
     1201 tgttgaacgt ggagagcgcg aaatcggaca aaaatcacgg ctgacgaggc ttgcagagga
     1261 gtaagccagt atacactccg ctagcgctac gtgactgggt cagggcttcg ccccgacacc
     1321 cccaaagggc gttgcgtttc acgcaacacc cttgcctaga atagatcttt tacgccgatt
     1381 tgtaagttgt tgttttttta gtgtgttttt tatttacctg tcgcatccag ggtgacacaa
     1441 cgttgtcgca tcctggatgc gacaacacgc aacaaggtgt tgcacggttg tgtcatgatg
     1501 gcttaaattt gaaccaacgt tagtgcatgg gattttcaga gggaaaaaat c
//
