LOCUS       NZ_CP058622             9135 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain JS316 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP058622
VERSION     NZ_CP058622.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15455438
            Assembly: GCF_014826015.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Sommer,J., Krause,F.F., Gerbracht,K.M., Wild,F., Tietgen,M.,
            Riedel-Christ,S., Sattler,J., Hamprecht,A., Kemp,V.A.J. and
            Goetting,S.
  TITLE     Sequencing of carbapenem resistant Enterobacterales
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   Sommer,J., Krause,F.F., Gerbracht,K.M., Wild,F., Tietgen,M.,
            Riedel-Christ,S., Sattler,J., Hamprecht,A., Kemp,V.A.J. and
            Goetting,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2020) Institute for Medical Microbiology and
            Infection Control, University Hospital Frankfurt, Paul-Ehrlich-Str
            40, Frankfurt 60596, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/15/2023 02:14:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,726
            Genes (coding)                    :: 4,492
            CDSs (with protein)               :: 4,492
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 64 of 234
            Pseudo Genes (incomplete)         :: 154 of 234
            Pseudo Genes (internal stop)      :: 42 of 234
            Pseudo Genes (multiple problems)  :: 27 of 234
            Pseudo Genes (short protein)      :: 4 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058622.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="JS316"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="Germany"
                     /lat_lon="50.1239 N 8.5763 E"
                     /collection_date="2019-12"
                     /collected_by="Institute for Medical Microbiology and
                     Infection Control, University Hospital Frankfurt"
     gene            complement(<57..737)
                     /gene="intI1"
                     /locus_tag="HZF20_RS23925"
                     /old_locus_tag="HZF20_23885"
                     /pseudo=""
     CDS             complement(<57..737)
                     /gene="intI1"
                     /locus_tag="HZF20_RS23925"
                     /old_locus_tag="HZF20_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /name="intI1"
     ARG             770..1368
                     /name="dfrA17"
                     /gene="dfrA17"
                     /gene_name="trimethoprim resistant dihydrofolate reductase
                     dfr"
                     /tool="rgi"
                     /drug_class="diaminopyrimidine antibiotic"
                     /identity="99.36"
                     /coverage="126.75"
     ARG             896..1365
                     /name="dfrA17"
                     /gene="dfrA17"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1499..2287
                     /locus_tag="HZF20_RS23935"
                     /old_locus_tag="HZF20_23895"
     CDS             1499..2287
                     /locus_tag="HZF20_RS23935"
                     /old_locus_tag="HZF20_23895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="WP_000503573.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKPD
                     SDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYPAR
                     RELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKEHFS
                     KALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPAEHRP
                     LICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
                     /name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
     gene            2493..2840
                     /locus_tag="HZF20_RS23940"
                     /old_locus_tag="HZF20_23900"
     CDS             2493..2840
                     /locus_tag="HZF20_RS23940"
                     /old_locus_tag="HZF20_23900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="WP_000679427.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAFY
                     FLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSP
                     SWKSLRRPTPW"
                     /name="quaternary ammonium compound efflux SMR transporter
                     QacE delta 1"
     gene            2834..>3421
                     /gene="folP"
                     /locus_tag="HZF20_RS23945"
                     /old_locus_tag="HZF20_23905"
                     /pseudo=""
     CDS             2834..>3421
                     /gene="folP"
                     /locus_tag="HZF20_RS23945"
                     /old_locus_tag="HZF20_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727244.1"
                     /GO_function="GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /name="folP"
     gene            3487..4191
                     /locus_tag="HZF20_RS23950"
                     /old_locus_tag="HZF20_23910"
     CDS             3487..4191
                     /locus_tag="HZF20_RS23950"
                     /old_locus_tag="HZF20_23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            4320..5084
                     /locus_tag="HZF20_RS23955"
                     /old_locus_tag="HZF20_23915"
     CDS             4320..5084
                     /locus_tag="HZF20_RS23955"
                     /old_locus_tag="HZF20_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="WP_001389365.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDHT
                     TIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTIDFY
                     LSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETAHRQ
                     VKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQPGIR
                     GEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
                     /name="IS6-like element IS6100 family transposase"
     gene            complement(5577..6161)
                     /gene="mphR(A)"
                     /locus_tag="HZF20_RS23960"
                     /old_locus_tag="HZF20_23920"
     CDS             complement(5577..6161)
                     /gene="mphR(A)"
                     /locus_tag="HZF20_RS23960"
                     /old_locus_tag="HZF20_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="WP_001137892.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAALI
                     QRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVNYL
                     ISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVDPDG
                     ELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
                     /name="mphR(A)"
     gene            complement(6161..7399)
                     /gene="mrx(A)"
                     /locus_tag="HZF20_RS23965"
                     /old_locus_tag="HZF20_23925"
                     /gene_synonym="mrx"
     CDS             complement(6161..7399)
                     /gene="mrx(A)"
                     /locus_tag="HZF20_RS23965"
                     /old_locus_tag="HZF20_23925"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="WP_000004159.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWAG
                     ATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTGLI
                     ACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGAAIG
                     LLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLLRPLF
                     GIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVGARISA
                     QNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRTPPELLG
                     RVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQFATMAAA
                     ATASAPTHTEGEH"
                     /name="mrx(A)"
     gene            complement(7396..8301)
                     /locus_tag="HZF20_RS23970"
                     /old_locus_tag="HZF20_23930"
     CDS             complement(7396..8301)
                     /locus_tag="HZF20_RS23970"
                     /old_locus_tag="HZF20_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000219391.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDD
                     GRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMV
                     IQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDV
                     DRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSE
                     ARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFA
                     LDSGNEEYLAAAKAQLAAAE"
                     /name="Mph(A) family macrolide 2'-phosphotransferase"
     gene            complement(8423..9127)
                     /locus_tag="HZF20_RS23975"
                     /old_locus_tag="HZF20_23935"
     CDS             complement(8423..9127)
                     /locus_tag="HZF20_RS23975"
                     /old_locus_tag="HZF20_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     ARG             2494..2837
                     /name="qacEdelta1"
                     /gene="qacEdelta1"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7400..8301)
                     /name="mph(A)"
                     /gene="mph(A)"
                     /gene_name="Mph(A) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1500..2284
                     /name="aadA5"
                     /gene="aadA5"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7397..8301)
                     /name="mphA"
                     /gene="mphA"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             2835..3475
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="76.34"
     ARG             complement(6162..7399)
                     /name="Mrx"
                     /gene="Mrx"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 ccatcagcaa aaggggatga taagtttatc accaccgact atttgcaaca gtgccgccag
       61 gaatgcccgg cgcgcggata cttccgctca agggcgtcgg gaagcgcaac gccgctgcgg
      121 ccctcggcct ggtccttcag ccaccatgcc cgtgcacgcg acagctgctc gcgcaggctg
      181 ggtgccaagc tctcgggtaa catcaaggcc cgatccttgg agcccttgcc ctcccgcacg
      241 atgatcgtgc cgtgatcgaa atccagatcc ttgacccgca gttgcaaacc ctcactgatc
      301 cgcatgcccg ttccatacag aagctgggcg aacaaacgat gctcgccttc cagaaaaccg
      361 aggatgcgaa ccacttcatc cggggtcagc accaccggca agcgccgcga cggccgaggt
      421 cttccgatct cctgaagcca gggcagatcc gtgcacagca ccttgccgta gaagaacagc
      481 aaggccgcca atgcctgacg atgcgtggag accgaaacct tgcgctcgtt cgccagccag
      541 gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac accgtggaaa
      601 cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaaact gtaatgcaag
      661 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      721 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg tacagtctat gcctcgggca
      781 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
      841 gttacgcagc agggcagtcg ccctaaaaca aagttagcca ttaagggagt taaattgaaa
      901 atatcattga tttctgcagt gtcagaaaat ggcgtaatcg gtagtggtcc tgatatcccg
      961 tggtcagtaa aaggtgagca actactcttt aaagcgctca catataatca atggctcctt
     1021 gtcggaagaa aaacatttga ctctatgggt gttcttccaa atcgcaaata tgcagtagtg
     1081 tcaaagaacg gaatttcaag ctcaaatgaa aacgtcctag tttttccttc aatagaaaat
     1141 gctttgaaag agctatcaaa agttacagat catgtatatg tctctggcgg gggtcaaatc
     1201 tataatagcc ttattgaaaa agcagatata attcatttgt ctactgttca cgttgaagtc
     1261 gaaggtgata tcaaattccc tataatgcct gagaatttca atttggtttt tgaacagttt
     1321 tttatgtcta atataaatta tacataccag atttggaaaa aaggctaaca atgcgttgca
     1381 gcaccagtcg cttcgctcct tggacagctt ttaagtcgcg tctttgtggt tttgctgcgc
     1441 aaaagtattc cacaaagccg caacttaaaa gctgccgctg aacttaacgt taggcatcat
     1501 gggtgaattt ttccctgcac aagttttcaa gcagctgtcc cacgctcgcg cggtgatcga
     1561 gcgccatctg gctgcgacac tggacacaat ccacctgttc ggatctgcga tcgatggagg
     1621 gctgaagccg gacagcgaca tagacttgct cgtgaccgtc agcgccgcac ctaacgattc
     1681 gctccggcag gcgctaatgc tcgatttgct gaaagtctca tcaccgccag gcgatggcgg
     1741 aacatggcga ccgctggagc taactgttgt cgctcgaagc gaagtagtgc cttggcgcta
     1801 tccggcgcgg cgtgagcttc agttcggtga gtggctccgc cacgacatcc tttccggaac
     1861 gttcgagcct gccgttctgg atcacgatct tgcgattttg ctgaccaagg cgaggcaaca
     1921 cagccttgcg cttctaggcc catccgcagc cacgtttttc gagccggtgc cgaaggagca
     1981 tttctccaag gcgcttttcg acactattgc ccagtggaat gcagagtcgg attggaaggg
     2041 tgacgagcgg aacgtcgttc ttgctcttgc tcgcatttgg tacagcgctt caactggtct
     2101 cattgctcct aaggacgttg ctgccgcatg ggtatcggag cgtttgcctg ccgagcatcg
     2161 gcccctcatc tgcaaggcac gcgcggcgta cctgggtagc gaggacgacg acctagcaat
     2221 gcgcgtcgaa gagacggccg cgttcgttcg atatgccaaa gcaacgattg agagaatctt
     2281 gcgttgagcg gcatgtgcga aaagtgcatc gacccgcgcc gagggcatct gatgcctaac
     2341 tcggcgttca agcggacggg ctgcgcccgc cgctcaacta tgcgttagat gcactaagca
     2401 cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca
     2461 taataagccc tacacaaatt gggagatata tcatgaaagg ctggcttttt cttgttatcg
     2521 caatagttgg cgaagtaatc gcaacatccg cattaaaatc tagcgagggc tttactaagc
     2581 ttgccccttc cgccgttgtc ataatcggtt atggcatcgc attttatttt ctttctctgg
     2641 ttctgaaatc catccctgtc ggtgttgctt atgcagtctg gtcgggactc ggcgtcgtca
     2701 taattacagc cattgcctgg ttgcttcatg ggcaaaagct tgatgcgtgg ggctttgtag
     2761 gtatggggct cataattgct gcctttttgc tcgcccgatc cccatcgtgg aagtcgctgc
     2821 ggaggccgac gccatggtga cggtgttcgg cattctgaat ctcaccgagg actccttctt
     2881 cgatgagagc cggcggctag accccgccgg cgctgtcacc gcggcgatcg aaatgctgcg
     2941 agtcggatca gacgtcgtgg atgtcggacc ggccgccagc catccggacg cgaggcctgt
     3001 atcgccggcc gatgagatca gacgtattgc gccgctctta gacgccctgt ccgatcagat
     3061 gcaccgtgtt tcaatcgaca gcttccaacc ggaaacccag cgctatgcgc tcaagcgcgg
     3121 cgtgggctac ctgaacgata tccaaggatt tcctgaccct gcgctctatc ccgatattgc
     3181 tgaggcggac tgcaggctgg tggttatgca ctcagcgcag cgggatggca tcgccacccg
     3241 caccggtcac cttcgacccg aagacgcgct cgacgagatt gtgcggttct tcgaggcgcg
     3301 ggtttccgcc ttgcgacgga gcggggtcgc tgccgaccgg ctcatcctcg atccggggat
     3361 gggatttttc ttgagccccg caccggaaac atcgctgcac gtgctgtcga accttcaaaa
     3421 gctggcactg ttgcaaatag tcggtggtga taaacttatc atcccctttt gctgatggag
     3481 ctgcacatga acccattcaa aggccggcat tttcagcgtg acatcattct gtgggccgta
     3541 cgctggtact gcaaatacgg catcagttac cgtgagctgc aggagatgct ggctgaacgc
     3601 ggagtgaatg tcgatcactc cacgatttac cgctgggttc agcgttatgc gcctgaaatg
     3661 gaaaaacggc tgcgctggta ctggcgtaac ccttccgatc tttgcccgtg gcacatggat
     3721 gaaacctacg tgaaggtcaa tggccgctgg gcgtatctgt accgggccgt cgacagccgg
     3781 ggccgcactg tcgattttta tctctcctcc cgtcgtaaca gcaaagctgc ataccggttt
     3841 ctgggtaaaa tcctcaacaa cgtgaagaag tggcagatcc cgcgattcat caacacggat
     3901 aaagcgcccg cctatggtcg cgcgcttgct ctgctcaaac gcgaaggccg gtgcccgtct
     3961 gacgttgaac accgacagat taagtaccgg aacaacgtga ttgaatgcga tcatggcaaa
     4021 ctgaaacgga taatcggcgc cacgctggga tttaaatcca tgaagacggc ttacgccacc
     4081 atcaaaggta ttgaggtgat gcgtgcacta cgcaaaggcc aggcctcagc attttattat
     4141 ggtgatcccc tgggcgaaat gcgcctggta agcagagttt ttgaaatgta aggcctttga
     4201 ataagacaaa aggctgcctc atcgctaact ttgcaacagt gccctcaata ctcgtgtggg
     4261 ctctgttgca aaaatcgtga agcttgagca tgcttggcgg agattggacg gacggaacga
     4321 tgacggattt caagtggcgc catttccagg gtgatgtgat cctgtgggcg gtgcgctggt
     4381 attgtcgcta tccgatcagc tatcgcgacc ttgaggaaat gctggcggaa cgcggcattt
     4441 cggtcgacca tacgacgatc tatcgctggg tccagtgcta cgccccggag atggagaagc
     4501 ggctgcgctg gttctggcgg cgtggctttg atccgagctg gcgcctggat gaaacctacg
     4561 tcaaggtgcg gggcaagtgg acctacctgt accgggcagt cgacaagcgg ggcgacacga
     4621 tcgatttcta cctgtcgccg acccgcagcg ccaaggcagc gaagcggttc ctgggcaagg
     4681 ccctgcgagg cctgaagcac tgggaaaagc ctgccacgct caataccgac aaagcgccga
     4741 gctatggtgc agcgatcacc gaattgaagc gcgaaggaaa gctggaccgg gagacggccc
     4801 accggcaggt gaagtatctc aataacgtga tcgaggccga tcacggaaag ctcaagatac
     4861 tgatcaagcc ggtgcgcggt ttcaaatcga tccccacggc ctatgccacg atcaagggat
     4921 tcgaagtcat gcgagccctg cgcaaaggac aggctcgccc ctggtgcctg cagcccggca
     4981 tcaggggcga ggtgcgcctt gtggagagag cttttggcat tgggccctcg gcgctgacgg
     5041 aggccatggg catgctcaac caccatttcg cagcagccgc ctgatcggcg cagagcgaca
     5101 gcctacctct gactgccgcc aatctttgca acagagcccg ccgtgctagt ctgctcggtg
     5161 atggtggagt gaagccaacc cgcaatcggg ttatgaatct gcatcgcgat tcgcaatcag
     5221 ctgtctcttg agcatgtcga actcctgcga tgttatctcg ccgtgctcgt gctgctacac
     5281 caacttgtcc aactcatccg ccacaccgct ccccaccacc tgcgatggcc aggttgtttg
     5341 ctgggcgcat tggcggagta tcgtctcgac ccgggacacc cacaccggca caaccttacg
     5401 ggagtgattc actgtcaaag aatcggcccg gtgctctgac gcaagtatcg ggatggtcac
     5461 catttgtaag ccgtagacct gagtggtgat caagacttcg ataccaccga ccgtaccggt
     5521 actaatcgac gacggtcgtg ttcgtcgcct gccgcaggga ctctgcacac ctccgtttac
     5581 gcatgtgcct ggaggagttg gaaatcgtcg tgttcgggaa acattaaaca caggatggca
     5641 gcgatctgag ccagcacatg atcagctagc tcaccatccg gatcgacggc ccactgcatc
     5701 gtcgcgccag cgatgaccga gtgcaggagc aactcagctg ccgcaggagc acctgggggc
     5761 agtcgcttgc ggatcccctc caccaccgcg cggttccgct ggatcgcaag cgtgcgtagc
     5821 tccggcacct ggagctcgta ccaggagatg agatagttca ccgagaagtc gttgcgagtg
     5881 ttcatgctcc gaacgagcac ctgcaaaaat tcccagagcc cttgcggccc tgcgcctatc
     5941 ggtatcgcat tcaggtaatg ccgcacctgc tcgacgccgc gctccatcat cctcaccagc
     6001 agcgtatcgc ggttggtgaa gcgctggatt aacgctgcgc gggagagccc cacctccttt
     6061 gctactccgc tgagcgtgaa ctctatggga ccgcaacgct tcagcactac ggtggcggcc
     6121 tcgagtacct cgtcatcgga cttgagcttg gggcggggca tcagtgttca ccttctgtat
     6181 gggttggggc ggaggctgtg gctgccgccg ccattgtagc aaattgaaga cggagcgaga
     6241 gtagagccac gagccccgca aacacggccg atacaacgag gccagggagc ggaccagcaa
     6301 ggtcgacaaa cgcgccggcc gcaagcataa ccatgggcga ggctgacagc atcaccgccg
     6361 agaccgtgcc gagtacccgg ccgagaagtt ctggcggcgt gcggttgtag atggcagcgt
     6421 tgagaatggg agagactgag ccggtcagca gtcccacgag cgcgcccaac aacatcagca
     6481 ccggcacgcc tggcaactgt gaaagcagaa gcgagcccac cgcagagcca caaaatgcca
     6541 ccgccagcca gttctgcgct gatatccggg cgccgaccga cgcatgaatg gcaatgccaa
     6601 ggagaccacc agcccccatc attgaggaga acagcccgag ctctgctact tggcgtcctg
     6661 catctacaaa cagcgcaggc atgatgacgc tgccgttggc gccaacgatg cccacgaaga
     6721 tcatcactat accaaagaga gggcgcagca ggggttcgct ccagagaaaa gcgacgccgg
     6781 cgcgcatgga gagagtcgcc gtcgtggtca tcgtccgagc ggcacgcgcg ggaagcaccc
     6841 acgcgccgag cagacctgca aggacggagc agaacgccgt cagcccgagc gttggcgcag
     6901 cgccaagcag gccgattgcg gcccccccaa gggccgggcc acctagaatc gcgacgttcc
     6961 cgatcaccgc tttcagtgac gagacgcgct caacggagag cccggcgacg tggccgagtt
     7021 tgggcagctc actgtcctgc gcggccatac cgggtgcgtc gaacgcggca ccgagcacca
     7081 cgcaagcgat cagcccagtg ttcgagaggg cgccaacggc atcgagcagt gggatgctcg
     7141 ccatggccac gccgcccacc acacccgaga tcaatgcgac gggcgcgcgc ccgaaccgat
     7201 cgacgaggcc accaccaacc cacgcgccga tgatggtcgc gatgacgctg ctagcggccg
     7261 tggcgcccgc ccaggccgcg ctctttgtat gagacaggac gaaccatgga agcgcgaggg
     7321 ccgccaccgc gttgccgatc cggaagagaa aggtcgccgc gaacagcgtc gcgagcgggc
     7381 tatatcgacg ttcgctcatt ccgctgcggc gagctgcgcc ttcgccgcag cgaggtactc
     7441 ttcgttaccc gagtcgaggg cgaagagtgc gtaggtgacc gccccgaacg caaggcgctc
     7501 cgcgatgtgg tgggcgagcc gcggccacac ccggccaccg gccgcttcat acgtgaggag
     7561 gagcttcgcg agcccctctt caccaaagac cataaggtgc gcggccatgt cgatggcagg
     7621 gtcatcaacg cgggcctcgc tccagtcgat catcccgctg acgcgctccg tgttgtcgat
     7681 gagcacatgg cccacgtaga gatcgccatg caccaccacg gagaaatctg gccacgacga
     7741 atcgtcgtcg agccagcgct gccaccggtg gaggcgcttg tcgttcacca cgaactcgcg
     7801 tcggacgcgg tcaacgtcgt cggccacctt ctgacgggcc tgcgtcggtg tacggatgag
     7861 catccccgca tccacggcgg cggaaatggg gacggcatgc agggcggcga gcgcggtcgc
     7921 gaagctctcc gcgaagacct ccgagtcctg cggcacgacc cagtcgggcg tggacgaacc
     7981 aggctggatg accatcgcag tcgagtcttc gagcatggga taggcaacga gctcggcgtt
     8041 ggccacgcgc cagtccggca ccgcgaacgg caggcgattc ttgagcattg ccagcacccg
     8101 cgcctctggt tcgaccttcg cgcttacctc ggctcggcgc gggatgcgca gcacccaccg
     8161 acgtccatcg tcgacggtgg cgatcacgat cctatagtcg agcccaagct cattgacagt
     8221 cagcgggcca tggagcttga gcccatgtcg ggctgcaagt gcgtacagtt gggaggtatc
     8281 ggcggtcgtg actacggtca tgattcactc ctgagggctt gacgggttta gccacctaaa
     8341 tgtaacagtc acgtcggtta tattcaatcc ggcactgttg caaagttagc gatgaggcag
     8401 ccttttgtct tattcaaagg ccttacattt caaaaactct gcttaccagg cgcatttcgc
     8461 ccaggggatc accataataa aatgctgagg cctggccttt gcgtagtgca cgcatcacct
     8521 caataccttt gatggtggcg taagccgtct tcatggattt aaatcccagc gtggcgccga
     8581 ttatccgttt cagtttgcca tgatcgcatt caatcacgtt gttccggtac ttaatctgtc
     8641 ggtgttcaac gtcagacggg caccggcctt cgcgtttgag cagagcaagc gcgcgaccat
     8701 aggcgggcgc tttatccgtg ttgatgaatc gcgggatctg ccacttcttc acgttgttga
     8761 ggattttacc cagaaaccgg tatgcagctt tgctgttacg acgggaggag agataaaaat
     8821 cgacagtgcg gccccggctg tcgacggccc ggtacagata cgcccagcgg ccattgacct
     8881 tcacgtaggt ttcatccatg tgccacgggc aaagatcgga agggttacgc cagtaccagc
     8941 gcagccgttt ttccatttca ggcgcataac gctgaaccca gcggtaaatc gtggagtgat
     9001 cgacattcac tccgcgttca gccagcatct cctgcagctc acggtaactg atgccgtatt
     9061 tgcagtacca gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg cctttgaatg
     9121 ggttcatgtg cagct
//
