LOCUS       NZ_CP058616             2473 bp    DNA     circular CON 13-SEP-2023
DEFINITION  Staphylococcus aureus strain 110900 plasmid unnamed, complete
            sequence.
ACCESSION   NZ_CP058616
VERSION     NZ_CP058616.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15430096
            Assembly: GCF_013836525.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Sieber,R.N., Overballe-Petersen,S., Kaya,H., Larsen,A.R. and
            Petersen,A.
  TITLE     Complete Genome Sequences of Methicillin-Resistant Staphylococcus
            aureus (MRSA) Strains 110900 and 128254, two Representatives of the
            CRISPR-carrying ST630 / spa Type t4549 Lineage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Sieber,R.N., Overballe-Petersen,S., Kaya,H., Larsen,A.R. and
            Petersen,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2020) Department of Bacteria, Parasites and Fungi,
            Statens Serum Institut, Artillerivej 5, Copenhagen 2300, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 334x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/13/2023 02:30:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,875
            Genes (coding)                    :: 2,768
            CDSs (with protein)               :: 2,768
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 52 of 107
            Pseudo Genes (incomplete)         :: 53 of 107
            Pseudo Genes (internal stop)      :: 28 of 107
            Pseudo Genes (multiple problems)  :: 21 of 107
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058616.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="110900"
                     /isolation_source="UBERON:0002067"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed"
                     /geo_loc_name="Denmark"
                     /lat_lon="56.189472 N 10.172893 E"
                     /collection_date="2016-09-02"
     gene            228..704
                     /locus_tag="HZR12_RS14700"
                     /old_locus_tag="HZR12_14700"
     CDS             228..704
                     /locus_tag="HZR12_RS14700"
                     /old_locus_tag="HZR12_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000664727.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            1399..1458
                     /gene="ermCL"
                     /locus_tag="HZR12_RS14705"
                     /old_locus_tag="HZR12_14705"
     CDS             1399..1458
                     /gene="ermCL"
                     /locus_tag="HZR12_RS14705"
                     /old_locus_tag="HZR12_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WP_002497446.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     gene            1519..2253
                     /gene="erm(C)"
                     /locus_tag="HZR12_RS14710"
                     /old_locus_tag="HZR12_14710"
     ARG             1520..2250
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1520..2253
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          229..714
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtagcattta cgttagataa ccccctgata tgctccgacg ctttatatag aaaagaagat
       61 tcaactaggt aaaatcttaa tataggttga gatgataagg tttataagga atttgtttgt
      121 tctaattttt cactcatttt gttctaattt cttttaacaa atgttctttt ttttttagaa
      181 cagttatgat atagttagaa tagtttaaaa taaggagtga gaaaaagatg aaagaaagat
      241 atggaacagt ctataaaggc tctcagaggc tcatagacga agaaagtgga gaagtcatag
      301 aggtagacaa gttataccgt aaacaaacgt ctggtaactt cgtaaaggca tatatagtgc
      361 aattaataag tatgttagat atgattggcg gaaaaaaact taaaatcgtt aactatatcc
      421 tagataatgt ccacttaagt aacaatacaa tgatagctac aacaagagaa atagcaaaag
      481 ctacaggaac aagtctacaa acagtaataa caacacttaa aatcttagaa gaaggaaata
      541 ttataaaaag aaaaactgga gtattaatgt taaaccctga actactaatg agaggcgacg
      601 accaaaaaca aaaatacctc ttactcgaat ttgggaactt tgagcaagag gcaaatgaaa
      661 aacaagaaaa tgcattatct gattattatt ctttcaagga ctagtataac ataaaatcgt
      721 ctacaaatag acaaaaaacc tgcacgctta atgtagatca aaagcttaac gcaaatgaaa
      781 tagattgacc tcccaataac accacgtagt tattgggagg tcaatctatg aaatgcgatt
      841 aagctttttc taattcacat aagcgtgcag gtttaaagta cataaaaaat ataatgaaaa
      901 aaagcatcat tatactaacg ttataccaac attatactct cattatacta attgcttatt
      961 ccaatttcct attggttgga accaacaggc gttagtgtgt tgttgagttg gtactttcat
     1021 gggattaatc ccatgaaacc cccaaccaac tcgccaaagc tttggctaac acacacgcca
     1081 ttccaaccaa tagttttctc ggcataaagc catgctctga cgcttaaatg cactaatgcc
     1141 ttaaaaaaac attaaagtct aacacactag acttatttac ttcgtaatta agtcgttaaa
     1201 ccgtgtgctc tacgaccaaa agtataaaac ctttaagaac tttctttttt cttgtaaaaa
     1261 aagaaactag ataaatctct catatctttt attcaataat cgcatcagat tgcagtataa
     1321 atttaacgat cactcatcat gttcatattt atcagagctc gtgctataat tatactaatt
     1381 ttataaggag gaaaaaatat gggcattttt agtatttttg taatcagcac agttcattat
     1441 caaccaaaca aaaaataagt ggttataatg aatcgttaat aagcaaaatt cattataacc
     1501 aaattaaaga gggttataat gaacgagaaa aatataaaac acagtcaaaa ctttattact
     1561 tcaaaacata atatagataa aataatgaca aatataagat taaatgaaca tgataatatc
     1621 tttgaaatcg gctcaggaaa agggcatttt acccttgaat tagtacagag gtgtaatttc
     1681 gtaactgcca ttgaaataga ccataaatta tgcaaaacta cagaaaataa acttgttgat
     1741 cacgataatt tccaagtttt aaacaaggat atattgcagt ttaaatttcc taaaaaccaa
     1801 tcctataaaa tatttggtaa tataccttat aacataagta cggatataat acgcaaaatt
     1861 gtttttgata gtatagctga tgagatttat ttaatcgtgg aatacgggtt tgctaaaaga
     1921 ttattaaata caaaacgctc attggcatta tttttaatgg cagaagttga tatttctata
     1981 ttaagtatgg ttccaagaga atattttcat cctaaaccta aagtgaatag ctcacttatc
     2041 agattaaata gaaaaaaatc aagaatatca cacaaagata aacagaagta taattatttc
     2101 gttatgaaat gggttaacaa agaatacaag aaaatattta caaaaaatca atttaacaat
     2161 tccttaaaac atgcaggaat tgacgattta aacaatatta gctttgaaca attcttatct
     2221 cttttcaata gctataaatt atttaataag taagttaagg gatgcagttt atgcatccct
     2281 taacttgttt ttcgtgtacc tattttttgt gaatcgatta tgtcttttgc gcattcactt
     2341 cttttctata taaatatgag cgaagcgaat aagcgtcgga aaagcagcaa aaagtttcct
     2401 ttttgctgtt ggagcatggg ggttcagggg gtgcagtatc tgacgtcaat gccgagcgaa
     2461 agcgagccga agg
//
