LOCUS       NZ_CP058614             2473 bp    DNA     circular CON 13-SEP-2023
DEFINITION  Staphylococcus aureus strain 128254 plasmid unnamed, complete
            sequence.
ACCESSION   NZ_CP058614
VERSION     NZ_CP058614.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15430097
            Assembly: GCF_013836745.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Sieber,R.N., Overballe-Petersen,S., Kaya,H., Larsen,A.R. and
            Petersen,A.
  TITLE     Complete Genome Sequences of Methicillin-Resistant Staphylococcus
            aureus (MRSA) Strains 110900 and 128254, two Representatives of the
            CRISPR-carrying ST630 / spa Type t4549 Lineage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Sieber,R.N., Overballe-Petersen,S., Kaya,H., Larsen,A.R. and
            Petersen,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2020) Department of Bacteria, Parasites and Fungi,
            Statens Serum Institut, Artillerivej 5, Copenhagen 2300, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 219x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/13/2023 02:41:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,729
            CDSs (with protein)               :: 2,729
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 50 of 108
            Pseudo Genes (incomplete)         :: 54 of 108
            Pseudo Genes (internal stop)      :: 24 of 108
            Pseudo Genes (multiple problems)  :: 17 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058614.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="128254"
                     /isolation_source="UBERON:0002067"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.733467 N 12.441296 E"
                     /collection_date="2018-11-06"
     gene            complement(121..855)
                     /gene="erm(C)"
                     /locus_tag="HZR13_RS14510"
                     /old_locus_tag="HZR13_14510"
     ARG             complement(125..855)
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(916..975)
                     /gene="ermCL"
                     /locus_tag="HZR13_RS14515"
                     /old_locus_tag="HZR13_14515"
     CDS             complement(916..975)
                     /gene="ermCL"
                     /locus_tag="HZR13_RS14515"
                     /old_locus_tag="HZR13_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WP_002497446.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     gene            complement(1670..2146)
                     /locus_tag="HZR13_RS14520"
                     /old_locus_tag="HZR13_14520"
     CDS             complement(1670..2146)
                     /locus_tag="HZR13_RS14520"
                     /old_locus_tag="HZR13_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000664727.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     ARG             complement(122..855)
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          complement(1661..2146)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttattcgct tcgctcatat ttatatagaa aagaagtgaa tgcgcaaaag acataatcga
       61 ttcacaaaaa ataggtacac gaaaaacaag ttaagggatg cataaactgc atcccttaac
      121 ttacttatta aataatttat agctattgaa aagagataag aattgttcaa agctaatatt
      181 gtttaaatcg tcaattcctg catgttttaa ggaattgtta aattgatttt ttgtaaatat
      241 tttcttgtat tctttgttaa cccatttcat aacgaaataa ttatacttct gtttatcttt
      301 gtgtgatatt cttgattttt ttctatttaa tctgataagt gagctattca ctttaggttt
      361 aggatgaaaa tattctcttg gaaccatact taatatagaa atatcaactt ctgccattaa
      421 aaataatgcc aatgagcgtt ttgtatttaa taatctttta gcaaacccgt attccacgat
      481 taaataaatc tcatcagcta tactatcaaa aacaattttg cgtattatat ccgtacttat
      541 gttataaggt atattaccaa atattttata ggattggttt ttaggaaatt taaactgcaa
      601 tatatccttg tttaaaactt ggaaattatc gtgatcaaca agtttatttt ctgtagtttt
      661 gcataattta tggtctattt caatggcagt tacgaaatta cacctctgta ctaattcaag
      721 ggtaaaatgc ccttttcctg agccgatttc aaagatatta tcatgttcat ttaatcttat
      781 atttgtcatt attttatcta tattatgttt tgaagtaata aagttttgac tgtgttttat
      841 atttttctcg ttcattataa ccctctttaa tttggttata atgaattttg cttattaacg
      901 attcattata accacttatt ttttgtttgg ttgataatga actgtgctga ttacaaaaat
      961 actaaaaatg cccatatttt ttcctcctta taaaattagt ataattatag cacgagctct
     1021 gataaatatg aacatgatga gtgatcgtta aatttatact gcaatctgat gcgattattg
     1081 aataaaagat atgagagatt tatctagttt ctttttttac aagaaaaaag aaagttctta
     1141 aaggttttat acttttggtc gtagagcaca cggtttaacg acttaattac gaagtaaata
     1201 agtctagtgt gttagacttt aatgtttttt taaggcatta gtgcatttaa gcgtcagagc
     1261 atggctttat gccgagaaaa ctattggttg gaatggcgtg tgtgttagcc aaagctttgg
     1321 cgagttggtt gggggtttca tgggattaat cccatgaaag taccaactca acaacacact
     1381 aacgcctgtt ggttccaacc aataggaaat tggaataagc aattagtata atgagagtat
     1441 aatgttggta taacgttagt ataatgatgc tttttttcat tatatttttt atgtacttta
     1501 aacctgcacg cttatgtgaa ttagaaaaag cttaatcgca tttcatagat tgacctccca
     1561 ataactacgt ggtgttattg ggaggtcaat ctatttcatt tgcgttaagc ttttgatcta
     1621 cattaagcgt gcaggttttt tgtctatttg tagacgattt tatgttatac tagtccttga
     1681 aagaataata atcagataat gcattttctt gtttttcatt tgcctcttgc tcaaagttcc
     1741 caaattcgag taagaggtat ttttgttttt ggtcgtcgcc tctcattagt agttcagggt
     1801 ttaacattaa tactccagtt tttcttttta taatatttcc ttcttctaag attttaagtg
     1861 ttgttattac tgtttgtaga cttgttcctg tagcttttgc tatttctctt gttgtagcta
     1921 tcattgtatt gttacttaag tggacattat ctaggatata gttaacgatt ttaagttttt
     1981 ttccgccaat catatctaac atacttatta attgcactat atatgccttt acgaagttac
     2041 cagacgtttg tttacggtat aacttgtcta cctctatgac ttctccactt tcttcgtcta
     2101 tgagcctctg agagccttta tagactgttc catatctttc tttcatcttt ttctcactcc
     2161 ttattttaaa ctattctaac tatatcataa ctgttctaaa aaaaaaagaa catttgttaa
     2221 aagaaattag aacaaaatga gtgaaaaatt agaacaaaca aattccttat aaaccttatc
     2281 atctcaacct atattaagat tttacctagt tgaatcttct tttctatata aagcgtcgga
     2341 gcatatcagg gggttatcta acgtaaatgc tacccttcgg ctcgctttcg ctcggcattg
     2401 acgtcagata ctgcaccccc tgaaccccca tgctccaaca gcaaaaagga aactttttgc
     2461 tgcttttccg acg
//
