LOCUS       NZ_CP058577            14294 bp    DNA     circular CON 18-AUG-2023
DEFINITION  Escherichia coli strain EC1 plasmid pEC1-3, complete sequence.
ACCESSION   NZ_CP058577
VERSION     NZ_CP058577.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15410927
            Assembly: GCF_013402895.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14294)
  AUTHORS   Mowlaboccus,S., Daley,D., Pang,S., Gottlieb,T., Merlino,J.,
            Nimmo,G.R., George,N., Korman,T.M., Streitberg,R., Robson,J.,
            Peachey,G., Collignon,P., Bradbury,S., Colombi,E., Ramsay,J.P.,
            Rogers,B.A. and Coombs,G.W.
  TITLE     Identification and characterisation of fosfomycin resistance in
            Escherichia coli urinary tract infection isolates from Australia
  JOURNAL   Int J Antimicrob Agents 56 (4), 106121 (2020)
   PUBMED   32739475
REFERENCE   2  (bases 1 to 14294)
  AUTHORS   Ramsay,J.P., Colombi,E. and Mowlaboccus,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2020) School of Pharmacy & Biomedical Sciences,
            Curtin University, Kent street, Bentley, WA 6102, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-FEB-2020
            Assembly Method :: Flye v. 2.6; Racon v. 1.4.15; Pilon v. 1.23
            Assembly Name                     :: EC1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 626.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/18/2023 05:40:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,230
            CDSs (total)                      :: 5,111
            Genes (coding)                    :: 4,869
            CDSs (with protein)               :: 4,869
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 98 of 242
            Pseudo Genes (incomplete)         :: 159 of 242
            Pseudo Genes (internal stop)      :: 33 of 242
            Pseudo Genes (multiple problems)  :: 49 of 242
            Pseudo Genes (short protein)      :: 4 of 242
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058577.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC1"
                     /isolation_source="urinary tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC1-3"
                     /geo_loc_name="Australia"
                     /lat_lon="24.49 S 133.26 E"
                     /collection_date="2019"
                     /collected_by="Hospital staff"
     gene            1..936
                     /locus_tag="HYH05_RS25740"
                     /old_locus_tag="HYH05_25740"
     CDS             1..936
                     /locus_tag="HYH05_RS25740"
                     /old_locus_tag="HYH05_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666805.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            complement(1129..1527)
                     /locus_tag="HYH05_RS25745"
                     /old_locus_tag="HYH05_25745"
     CDS             complement(1129..1527)
                     /locus_tag="HYH05_RS25745"
                     /old_locus_tag="HYH05_25745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016249654.1"
                     /translation="MRISNVEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQTIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQELKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1704..2630
                     /locus_tag="HYH05_RS25750"
                     /old_locus_tag="HYH05_25750"
     CDS             1704..2630
                     /locus_tag="HYH05_RS25750"
                     /old_locus_tag="HYH05_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_113227874.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDGVSRENRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2633..3166
                     /locus_tag="HYH05_RS25755"
                     /old_locus_tag="HYH05_25755"
     CDS             2633..3166
                     /locus_tag="HYH05_RS25755"
                     /old_locus_tag="HYH05_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011666803.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAIEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3220..3441)
                     /locus_tag="HYH05_RS25760"
                     /old_locus_tag="HYH05_25760"
     CDS             complement(3220..3441)
                     /locus_tag="HYH05_RS25760"
                     /old_locus_tag="HYH05_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144240.1"
                     /translation="MTKLDENIPHIIDAAVDFMASSQAFREYLKKLPPDNTIPPDLTDE
                     HVPLYIRRLEYYRLLYRPKQTESYWKKR"
                     /name="hypothetical protein"
     gene            complement(3985..4331)
                     /locus_tag="HYH05_RS25765"
                     /old_locus_tag="HYH05_25765"
                     /pseudo=""
     CDS             complement(3985..4331)
                     /locus_tag="HYH05_RS25765"
                     /old_locus_tag="HYH05_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329508.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            4492..6150
                     /locus_tag="HYH05_RS25770"
                     /old_locus_tag="HYH05_25770"
     CDS             4492..6150
                     /locus_tag="HYH05_RS25770"
                     /old_locus_tag="HYH05_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372656.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_001529697.1"
                     /translation="MKIVIVGGVAGGASAAARARRLSEDVSIVVFERGSDVSFANCGLP
                     YHIGGKIPLRQSLILKTPEDFKSRFNIDVRIRHEVMSVDPVNKMVTVKNLTSGEVYTEE
                     WDRLLLSTGAAPVVPPLPGLQEAGVFTLRNLTDMDAIQAWIEQHNIAHTTLVGGGFIGL
                     EVMEALSERGISVTLLEMGEQVMAPVDPEMASALHQEIRSHGVDLRLRTALTEVLRTET
                     GFRVALSDGGFLQTDMVILAIGVKPESSLATGAGLAVGKRGGISVNACMQTSIPDIYAV
                     GDAVETPDLVFQEPANVPLAGPANRQGRIAADNMLDRHSLYHGSQGTSICKVFSLSIGS
                     VGANEKQLKAHGTRYEKVYVHAADHASYYPGATMISLKLLFSPDTGKILGAQASGKKGV
                     DKRIDVLAVVQRAGLTVNDLEHLELTYAPPFNSARDVVNQAGMVAANVIKGDTVICHVR
                     DVLQREPGSYCLLDIRSPAELKQFGEYPDALSIPLDSLRENLDKLPEDKEIIIGCQSGL
                     RGHVAYRMLQSHGFRTYNLSGGFITWQAVTESMSQ"
                     /name="FAD-dependent oxidoreductase"
     gene            <7322..7768
                     /locus_tag="HYH05_RS25775"
                     /old_locus_tag="HYH05_25775"
                     /pseudo=""
     CDS             <7322..7768
                     /locus_tag="HYH05_RS25775"
                     /old_locus_tag="HYH05_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7962..8360)
                     /locus_tag="HYH05_RS25780"
                     /old_locus_tag="HYH05_25780"
     CDS             complement(7962..8360)
                     /locus_tag="HYH05_RS25780"
                     /old_locus_tag="HYH05_25780"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016249654.1"
                     /translation="MRISNVEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQTIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQELKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            8537..9463
                     /locus_tag="HYH05_RS25785"
                     /old_locus_tag="HYH05_25785"
     CDS             8537..9463
                     /locus_tag="HYH05_RS25785"
                     /old_locus_tag="HYH05_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_113227874.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDGVSRENRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            9466..9999
                     /locus_tag="HYH05_RS25790"
                     /old_locus_tag="HYH05_25790"
     CDS             9466..9999
                     /locus_tag="HYH05_RS25790"
                     /old_locus_tag="HYH05_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011666803.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAIEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(10053..10274)
                     /locus_tag="HYH05_RS25795"
                     /old_locus_tag="HYH05_25795"
     CDS             complement(10053..10274)
                     /locus_tag="HYH05_RS25795"
                     /old_locus_tag="HYH05_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144240.1"
                     /translation="MTKLDENIPHIIDAAVDFMASSQAFREYLKKLPPDNTIPPDLTDE
                     HVPLYIRRLEYYRLLYRPKQTESYWKKR"
                     /name="hypothetical protein"
     gene            complement(10818..11165)
                     /locus_tag="HYH05_RS25800"
                     /old_locus_tag="HYH05_25800"
     CDS             complement(10818..11165)
                     /locus_tag="HYH05_RS25800"
                     /old_locus_tag="HYH05_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329508.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_023144239.1"
                     /translation="MPMNEDTKRQTDMKNAALGAADVLRGLANSDRLLLMCQLSQGEAN
                     VTELEAAVGITQPTLSQQLGVMRRLKLVETRRAGKMVYYRIGDNRIMTLLNTLYDLYCP
                     QADPDKVKKQP"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            11326..12984
                     /locus_tag="HYH05_RS25805"
                     /old_locus_tag="HYH05_25805"
     CDS             11326..12984
                     /locus_tag="HYH05_RS25805"
                     /old_locus_tag="HYH05_25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372656.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_001529697.1"
                     /translation="MKIVIVGGVAGGASAAARARRLSEDVSIVVFERGSDVSFANCGLP
                     YHIGGKIPLRQSLILKTPEDFKSRFNIDVRIRHEVMSVDPVNKMVTVKNLTSGEVYTEE
                     WDRLLLSTGAAPVVPPLPGLQEAGVFTLRNLTDMDAIQAWIEQHNIAHTTLVGGGFIGL
                     EVMEALSERGISVTLLEMGEQVMAPVDPEMASALHQEIRSHGVDLRLRTALTEVLRTET
                     GFRVALSDGGFLQTDMVILAIGVKPESSLATGAGLAVGKRGGISVNACMQTSIPDIYAV
                     GDAVETPDLVFQEPANVPLAGPANRQGRIAADNMLDRHSLYHGSQGTSICKVFSLSIGS
                     VGANEKQLKAHGTRYEKVYVHAADHASYYPGATMISLKLLFSPDTGKILGAQASGKKGV
                     DKRIDVLAVVQRAGLTVNDLEHLELTYAPPFNSARDVVNQAGMVAANVIKGDTVICHVR
                     DVLQREPGSYCLLDIRSPAELKQFGEYPDALSIPLDSLRENLDKLPEDKEIIIGCQSGL
                     RGHVAYRMLQSHGFRTYNLSGGFITWQAVTESMSQ"
                     /name="FAD-dependent oxidoreductase"
     gene            complement(13477..13794)
                     /locus_tag="HYH05_RS25810"
                     /old_locus_tag="HYH05_25810"
     CDS             complement(13477..13794)
                     /locus_tag="HYH05_RS25810"
                     /old_locus_tag="HYH05_25810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_179226639.1"
                     /translation="MFATAPKYPEADKKQLALSVPDKKTTKVGNPRETIGVRLSQNIDN
                     EIVTQINLAASLILIQYSIKNARIINHIYKDIYQTKQPSSQLRIRIFLYVIDKTQFFYK
                     L"
                     /name="hypothetical protein"
     Typing          2..936
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.902"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7316..7768
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.357"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1705..2627
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8538..9460
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgaagaa aatttacccc accgtcccta tcacacggat
       61 gatctcgctt tcggccttcg tatctccggc aaagggcgcg cgcttcttgc acggtatatc
      121 cagcagaacc agcctcatgc acagttctgg ctggtttttg acgttgaccg cgagggggct
      181 gcgattgact ggagcgaccg gaacgctccc gcgccgaaca tcaccgttaa aaatcctgtg
      241 aacggacacg ctcacctgct ctacgcactc aacatcgccg tgagaaccgc gcctgatgcc
      301 tcggttaagg cgctgaaata tgccgcagcg gttgaacgtt cgctgtgtga gaaactttgt
      361 gcggatgtga actacagcgg cctgatttgc aaaaatccgt tccacctgga atggctggtg
      421 atggagtggc gcgaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc
      481 gcctcagcgc gtcgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggccagc attctcacag
      601 tggcttgatg ccgtgattca gcgtgtcgaa atgtacaacg catcgcttcc cgttccgctt
      661 tcacctcctg aatgtcgggc tattggcaag agtattgcga aatacacgca caggaacttc
      721 acgccggaaa ctttcgcaca gtatgtggct gatacgcaca cgccagaaat acaggccaag
      781 agaggcagga aaggtggcat cgctaaaggc gaagcctacg atgacaagcg tttcatggcg
      841 ctatgtatgc tggagaatgg atattctcag aaagctattg cggcgatgtt ggaggtttct
      901 actcgaacca ttcgaaactg gaaaagcgga aaatagccta tatcagataa cagcgccttt
      961 ctggcgttct tttgagcagc aggtcttttg ccgtttttcc cgttcagccc tgcaatggtg
     1021 ctgttttccg catgattgtg ctggtagtgc tcgccgcagt ctcatgaccg agcgtagcga
     1081 gcgaatgagc gaggaagcac cagggaaccg gaacacggat ggaggcactt acgcgtctgg
     1141 gtttggggat gtctccagat cgtacttaat ttccttaagt tcctgtaata tcctgtcttt
     1201 tttatagcat agctcgcctg tttttgtgtc taccagtcct gcttcgatca gtagcccagc
     1261 cgcgagaaag agtcggtgat tgcgttcccg tcgttgtttt tttccctcga ttcgtttctg
     1321 gatcgtctgt tttcgctcgt tctcctgtgc ctgtaagtcg tttttttccg ctgttgccag
     1381 cacgtggaga agtttgcgtt cctgttctgt gagtccggct tcgttgtcga gcaggtcaat
     1441 aatctgtctc tgtcgcgcgg tctgttctcc gagcttcctg atgaatccga tccgtttctt
     1501 cagccattcg acgttgctga tgcgcatgtt tgtggtactc cctggtgttt ttttacggcg
     1561 gtttgtggtc gtgcagtata tcgcttgcgg tcggcgggtg cagggggtat cttccgtgct
     1621 aattcgaata attagaagga agcgcactta tatgtttctc tccttcgtcg agaaacagtg
     1681 cgctcctgcg gagggctgaa accgtggcaa cctatcacct cagcgttaaa tttggtggca
     1741 aggggcaggc tgcaaaccac gccgattaca tcgagcgcaa agagaaatac cgcgaccgtc
     1801 aggatttgga atattccgca cacggaaaca tgccggaatg ggccagggat aatccttccc
     1861 atttctggca ggcggctgac cagtttgaac gtgcgaacgg ttcgacctat agggaacttg
     1921 aaattgcatt accccgtgaa ctgacaccgg aacagcgcct tgaactggtg caggattttg
     1981 tacgtcagga agccggcgaa cggcacgcct ggagttttgc cattcacaat ccgaaagcga
     2041 gcattgacgg cggcgaacag cctcatgcgc atatcatgat gagtcagcgt gtgaatgatg
     2101 gcattgaccg tacaccggaa cagtatttcc ggcgctataa cgccaggtat cccgagcggg
     2161 gaggtgcgaa aaaggattcc ggaagcctga ctcccactca gcaaaaagag cagttgcggg
     2221 aattgcgaaa acgctgggag gtgaagcaca acgaacatat gagaaagcat ggctttgaaa
     2281 ggggcttcat tgactgccgg acactgaaag aacagggcat tgaacgacgt cccgaagtgc
     2341 atctgggttt tgaagctgcc ggacgacttg atgatgccca gcgtcatgac atcatgcaaa
     2401 aacggtctga accggactat tcccgccatc ttgaacaggc gcgaatggtc gggcatgaca
     2461 tcagggaatg gctggataca ccggttaagc aacatgaact ggtcagaagg ctgcaacatg
     2521 atgttgcgcg ggagcatggc tttgacatga gagacggagt gagcagagag aaccgaactg
     2581 aacctgaacg ggttaaatca ccgtcacgtg gattttctat gggattctga acatggccag
     2641 cacagaagaa aaattactca cgctcctgtt atcggtggag catctgcaac ataccgcaat
     2701 agagcaacag aaagctgttg ctgattctct ggctgtggca agggaaactc ttcgcggata
     2761 tcgggatatt tctgacggtg tggcaaaaca ggtcagggat gaagtcagaa acgaggtgca
     2821 gaagctggac gtggcaggac tgataggtga gcgcatcagc caggattttc agcggctgga
     2881 agagtcagtc gcaaacatga aggcaaacgc ggatgccctg aacagggata ttgcgacaat
     2941 tcacagcaat atcgtgcagg aatacaacac ccttaaaggg tatagctgga aatggctggg
     3001 tggggtgtca gtggcttttc tggttatgtt ctgtgtgctc ggatggggaa tgaaaacaat
     3061 actcgacagg aactacaacg ccacagcggc ggtgtatcag aaagttgtga cactggaaga
     3121 taaactggtg gagaaacctg tcacggggaa gagccggaaa aactgacgcg cttcgcttgt
     3181 ccaacgcctc acggagcgcg aaaggatttc gcgaaccgtt caccgttttt tccaatatga
     3241 ttctgtctgc tttggtcgat aaagcagtcg atagtattcc agccttcgta tatataacgg
     3301 tacgtgctca tcagtaagat ctggcggtat tgtgttatca ggggggagtt ttttcagata
     3361 ctcacgaaat gcctggcttg atgccataaa atctacagct gcatcgataa tatgtgggat
     3421 attttcatct aattttgtca tgatgattga tctgatatta tcacggttcg tgaattatag
     3481 tatgtcacta ttatttatgc gttagtgaac cagaaaaaat cccccgttgg tataggggga
     3541 tatgtaagga gaaataactt cgcaggtgtt cttgtttgta ttataacgtg aaaagaatgc
     3601 acgcaactta attaatctgc cgtacttcct ttcagttttc aagataaggt ttataataaa
     3661 acatggaatg gaagcaagct ttaaggtttc agctctaaaa tttttaatat ctattaacta
     3721 gctagttgtt gtccttgtga gcatgttgta tataaaagct aggataatat cctgaccgga
     3781 gtattcatat tccttatccc ctctgtgttc tcagaggttt ttttctgtat ttaatgatat
     3841 gagctggaat gcgattccgg cccccccccg tttacataat tctccctgac atacggcacc
     3901 ggaatcgcat tgattttgca ttgttaatgt ctaactgtgc tggtgaatgt tgggggcagt
     3961 ttggatatcg aaaattattg tacgttaagg ttgttttttg accttgtcgg ggtccgcctg
     4021 cgggcagtag agatcatata aggtattcaa taacgtcatg atgcggttat ccccgatacg
     4081 gtaatatacc attttacctg cccgtcgggt ctcgaccagc ttcaggcgac gcatgacacc
     4141 caactgctga gacagggtcg gctgagtgat accgaccgcc gcttccagct cggtcacatt
     4201 cgcttcccct ggcttaactg acacatcaac aatagccggt cgctgttggc cagtccccgt
     4261 aggacatctg ctgcccccag ggctgcattt ttcatatcag tctgacgttt tgtgtcttca
     4321 ttcatgggca ttgctggttt cttatattat gtatttttat ataatattgt tcagtataat
     4381 gatagtggcg taattttgcc accgtgatct tcaggtctgc agcgtccagc tggaccgggt
     4441 aactaattat atatggcaga tattttgccc cgagtgaaca gagaggatgt aatgaaaata
     4501 gtgattgttg gtggtgttgc cgggggagca tccgctgcag ccagggctcg tcggttatca
     4561 gaggatgtca gtattgttgt gtttgagcgg ggaagcgatg tctctttcgc caactgtgga
     4621 ttgccttatc atataggcgg aaaaattccg ttacggcagt cgctgattct gaaaaccccg
     4681 gaggatttta aatcccgttt taacatagac gtccgtatcc gtcatgaagt gatgtcagta
     4741 gacccggtta ataagatggt gacggttaaa aatctgacct ccggggaggt ctataccgaa
     4801 gaatgggatc gtctgctcct cagtaccggt gccgctccgg ttgttccccc cttgcccgga
     4861 ctacaggaag cgggtgtctt tacactgcga aatcttactg acatggatgc tatccaggcg
     4921 tggattgagc aacataatat cgctcacacc acgcttgtcg gggggggatt tatcgggctc
     4981 gaagtgatgg aggcactgag cgaaaggggg atcagtgtga ccctgctgga gatgggggaa
     5041 caggttatgg ctccggttga tcctgaaatg gcatcggccc tgcatcagga aatcaggagt
     5101 catggcgtgg atctgcgtct cagaacggcg ctcaccgagg tactgcggac agagacggga
     5161 ttccgcgttg cactgtctga cggtgggttt ctgcagaccg acatggttat tctcgccatc
     5221 ggggtaaaac ctgagagcag ccttgccaca ggagccgggc tcgccgtggg taaacgggga
     5281 ggcataagtg tcaatgcctg tatgcagaca agtatccctg acatctatgc cgtgggggat
     5341 gcagttgaaa cacctgacct tgtctttcag gaacccgcta atgttcctct ggcagggcca
     5401 gccaaccgcc agggacgcat tgctgctgat aatatgctcg atcgtcacag cctttaccat
     5461 ggcagtcagg ggacatccat ttgtaaggta ttttcgctga gtattggcag tgtcggggcg
     5521 aatgaaaagc agctgaaagc ccatggtacc cgatacgaga aggtttacgt ccatgccgct
     5581 gatcatgcca gttactaccc gggggcaaca atgatcagtc tgaaactgct gttcagcccc
     5641 gataccggta aaattctcgg ggcccaggcg tcagggaaaa aaggggttga taaacgcatc
     5701 gatgtcctgg ctgtcgtaca gcgcgcgggg cttaccgtta acgatctcga gcatctggag
     5761 ctgacctatg ctccgccttt caacagtgca agggacgtcg ttaaccaggc tggaatggtg
     5821 gcggcaaatg ttatcaaggg cgatacggtc atctgtcatg tccgcgatgt gttacaacgg
     5881 gaaccgggca gttattgttt acttgatatt cgttctcctg cagaactgaa acaatttggt
     5941 gaatatcctg acgcgctatc catccctctt gattccctgc gggagaatct tgataagttg
     6001 cctgaagata aagagattat tatcgggtgt cagagtggtc tgcgggggca tgtggcatac
     6061 cgtatgcttc agtctcacgg cttccggaca tataatctga gtggtggctt tataacttgg
     6121 caggcggtta cggaatcaat gagtcaataa gagatgaggt gccatacaca tggcacctca
     6181 tcttccagat tcattgaaaa cgtattatat ggaatagaaa tctacaaaaa taattaacac
     6241 cctggatggt accctattgc gataatgtgg actgttcaaa aaacaggctt accgtacaat
     6301 aattctctat atccaaactg accccaatgt tcctgaatgt aaatctccgt acaaagatat
     6361 cgttgagttt tatataattc attgttttta ttgtgttttg ttttttttgt tgtgctggtt
     6421 tgtagttata aaaaacccct gggaatccag gggtaggtaa ggaaaacaaa tgatactcag
     6481 ttagtgttgc actttaaacg ggttgaagta taactcgtat taaatgagat gttgaaaata
     6541 agtgaaaacc attgctggtt gcaatagggc agtatgcgta aaaaataaat ttgtgatgct
     6601 tttcaaaaaa acaattaata catgaatatt acagctcata tattacagtt tataaaaaaa
     6661 ctgtgtttta tctattacat agagaaaaat tcttatcctg agctgtgaac ttggttgttt
     6721 cgtttgatat atatctttgt aaatgtggtt tattatgcgg gcatttttta ttgagtattg
     6781 aatcagaata agtgatgcag ctaaatttat ttgcgtaact atttcattgt caatattctg
     6841 actcaatctg acacctatcg tttctcttgg attccccact tttgtggttt ttttatccgg
     6901 aacgggatag tgccagttgt tttttatcag cctcagggta ttttggggcg gttgcgaaca
     6961 ttgacgcatt ccacaactaa aaaaatttct cacagactct gattcttgcc ccgacccttg
     7021 gtcggttttt ttttgtcctg gcgaaacgtg aagctctgaa acagaaaatc tccgggcaga
     7081 accttccatc cggggggaga aaacttttcg ttttgagccg ttcggtgttc agaacgcacg
     7141 aaaccgatcg cacatcgctt tcgtgcatag ttatgcaacg ccctgaaaac gattctgacg
     7201 cgtttttctg gttttgcctg gtgttttctt gtctttttgc gttttttgcg ccagaaagcg
     7261 actgagggcg ttttaagggg ggcgtacaac gggatttatg gtaaatggat cggtctcagc
     7321 gcgtcgtagc atcgataaac attacgggat ggggcgaaac tgccacctgt tcgaaatgac
     7381 gcgcaaatgg gcttacaggg cgattcgtca gggctggcca gcattctcac agtggcttga
     7441 tgccgtgatt cagcgtgtcg aaatgtacaa cgcatcgctt cccgttccgc tttcacctcc
     7501 tgaatgtcgg gctattggca agagtattgc gaaatacacg cacaggaact tcacgccgga
     7561 aactttcgca cagtatgtgg ctgatacgca cacgccagaa atacaggcca agagaggcag
     7621 gaaaggtggc atcgctaaag gcgaagccta cgatgacaag cgtttcatgg cgctatgtat
     7681 gctggagaat ggatattctc agaaagctat tgcggcgatg ttggaggttt ctactcgaac
     7741 cattcgaaac tggaaaagcg gaaaatagcc tatatcagat aacagcgcct ttctggcgtt
     7801 cttttgagca gcaggtcttt tgccgttttt ccccgttcag ccctgcaatg gtgctgtttt
     7861 ccgcatgatt gtgctggtag tgctcgccgc agtctcatga ccgagcgtag cgagcgaatg
     7921 agcgaggaag caccagggaa ccggaacacg gatggaggca cttacgcgtc tgggtttggg
     7981 gatgtctcca gatcgtactt aatttcctta agttcctgta atatcctgtc ttttttatag
     8041 catagctcgc ctgtttttgt gtctaccagt cctgcttcga tcagtagccc agccgcgaga
     8101 aagagtcggt gattgcgttc ccgtcgttgt ttttttccct cgattcgttt ctggatcgtc
     8161 tgttttcgct cgttctcctg tgcctgtaag tcgttttttt ccgctgttgc cagcacgtgg
     8221 agaagtttgc gttcctgttc tgtgagtccg gcttcgttgt cgagcaggtc aataatctgt
     8281 ctctgtcgcg cggtctgttc tccgagcttc ctgatgaatc cgatccgttt cttcagccat
     8341 tcgacgttgc tgatgcgcat gtttgtggta ctccctggtg tttttttacg gcggtttgtg
     8401 gtcgtgcagt atatcgcttg cggtcggcgg gtgcaggggg tatcttccgt gctaattcga
     8461 ataattagaa ggaagcgcac ttatatgttt ctctccttcg tcgagaaaca gtgcgctcct
     8521 gcggagggct gaaaccgtgg caacctatca cctcagcgtt aaatttggtg gcaaggggca
     8581 ggctgcaaac cacgccgatt acatcgagcg caaagagaaa taccgcgacc gtcaggattt
     8641 ggaatattcc gcacacggaa acatgccgga atgggccagg gataatcctt cccatttctg
     8701 gcaggcggct gaccagtttg aacgtgcgaa cggttcgacc tatagggaac ttgaaattgc
     8761 attaccccgt gaactgacac cggaacagcg ccttgaactg gtgcaggatt ttgtacgtca
     8821 ggaagccggc gaacggcacg cctggagttt tgccattcac aatccgaaag cgagcattga
     8881 cggcggcgaa cagcctcatg cgcatatcat gatgagtcag cgtgtgaatg atggcattga
     8941 ccgtacaccg gaacagtatt tccggcgcta taacgccagg tatcccgagc ggggaggtgc
     9001 gaaaaaggat tccggaagcc tgactcccac tcagcaaaaa gagcagttgc gggaattgcg
     9061 aaaacgctgg gaggtgaagc acaacgaaca tatgagaaag catggctttg aaaggggctt
     9121 cattgactgc cggacactga aagaacaggg cattgaacga cgtcccgaag tgcatctggg
     9181 ttttgaagct gccggacgac ttgatgatgc ccagcgtcat gacatcatgc aaaaacggtc
     9241 tgaaccggac tattcccgcc atcttgaaca ggcgcgaatg gtcgggcatg acatcaggga
     9301 atggctggat acaccggtta agcaacatga actggtcaga aggctgcaac atgatgttgc
     9361 gcgggagcat ggctttgaca tgagagacgg agtgagcaga gagaaccgaa ctgaacctga
     9421 acgggttaaa tcaccgtcac gtggattttc tatgggattc tgaacatggc cagcacagaa
     9481 gaaaaattac tcacgctcct gttatcggtg gagcatctgc aacataccgc aatagagcaa
     9541 cagaaagctg ttgctgattc tctggctgtg gcaagggaaa ctcttcgcgg atatcgggat
     9601 atttctgacg gtgtggcaaa acaggtcagg gatgaagtca gaaacgaggt gcagaagctg
     9661 gacgtggcag gactgatagg tgagcgcatc agccaggatt ttcagcggct ggaagagtca
     9721 gtcgcaaaca tgaaggcaaa cgcggatgcc ctgaacaggg atattgcgac aattcacagc
     9781 aatatcgtgc aggaatacaa cacccttaaa gggtatagct ggaaatggct gggtggggtg
     9841 tcagtggctt ttctggttat gttctgtgtg ctcggatggg gaatgaaaac aatactcgac
     9901 aggaactaca acgccacagc ggcggtgtat cagaaagttg tgacactgga agataaactg
     9961 gtggagaaac ctgtcacggg gaagagccgg aaaaactgac gcgcttcgct tgtccaacgc
    10021 ctcacggagc gcgaaaggat ttcgcgaacc gttcaccgtt ttttccaata tgattctgtc
    10081 tgctttggtc gataaagcag tcgatagtat tccagccttc gtatatataa cggtacgtgc
    10141 tcatcagtaa gatctggcgg tattgtgtta tcagggggga gttttttcag atactcacga
    10201 aatgcctggc ttgatgccat aaaatctaca gctgcatcga taatatgtgg gatattttca
    10261 tctaattttg tcatgatgat tgatctgata ttatcacggt tcgtgaatta tagtatgtca
    10321 ctattattta tgcgttagtg aaccagaaaa aatcccccgt tggtataggg ggatatgtaa
    10381 ggagaaataa cttcgcaggt gttcttgttt gtattataac gtgaaaagaa tgcacgcaac
    10441 ttaattaatc tgccgtactt cctttcagtt ttcaagataa ggtttataat aaaacatgga
    10501 atggaagcaa gctttaaggt ttcagctcta aaatttttaa tatctattaa ctagctagtt
    10561 gttgtccttg tgagcatgtt gtatataaaa gctaggataa tatcctgacc ggagtattca
    10621 tattccttat cccctctgtg ttctcagagg tttttttctg tatttaatga tatgagctgg
    10681 aatgcgattc cggccccccc ccgtttacat aattctccct gacatacggc accggaatcg
    10741 cattgatttt gcattgttaa tgtctaactg tgctggtgaa tgttgggggc agtttggata
    10801 tcgaaaatta ttgtacgtta aggttgtttt ttgaccttgt cggggtccgc ctgcgggcag
    10861 tagagatcat ataaggtatt caataacgtc atgatgcggt tatccccgat acggtaatat
    10921 accattttac ctgcccgtcg ggtctcgacc agcttcaggc gacgcatgac acccaactgc
    10981 tgagacaggg tcggctgagt gataccgacc gccgcttcca gctcggtcac attcgcttcc
    11041 ccctggctta actgacacat caacaatagc cggtcgctgt tggccagtcc ccgtaggaca
    11101 tctgctgccc ccagggctgc atttttcata tcagtctgac gttttgtgtc ttcattcatg
    11161 ggcattgctg gtttcttata ttatgtattt ttatataata ttgttcagta taatgatagt
    11221 ggcgtaattt tgccaccgtg atcttcaggt ctgcagcgtc cagctggacc gggtaactaa
    11281 ttatatatgg cagatatttt gccccgagtg aacagagagg atgtaatgaa aatagtgatt
    11341 gttggtggtg ttgccggggg agcatccgct gcagccaggg ctcgtcggtt atcagaggat
    11401 gtcagtattg ttgtgtttga gcggggaagc gatgtctctt tcgccaactg tggattgcct
    11461 tatcatatag gcggaaaaat tccgttacgg cagtcgctga ttctgaaaac cccggaggat
    11521 tttaaatccc gttttaacat agacgtccgt atccgtcatg aagtgatgtc agtagacccg
    11581 gttaataaga tggtgacggt taaaaatctg acctccgggg aggtctatac cgaagaatgg
    11641 gatcgtctgc tcctcagtac cggtgccgct ccggttgttc cccccttgcc cggactacag
    11701 gaagcgggtg tctttacact gcgaaatctt actgacatgg atgctatcca ggcgtggatt
    11761 gagcaacata atatcgctca caccacgctt gtcggggggg gatttatcgg gctcgaagtg
    11821 atggaggcac tgagcgaaag ggggatcagt gtgaccctgc tggagatggg ggaacaggtt
    11881 atggctccgg ttgatcctga aatggcatcg gccctgcatc aggaaatcag gagtcatggc
    11941 gtggatctgc gtctcagaac ggcgctcacc gaggtactgc ggacagagac gggattccgc
    12001 gttgcactgt ctgacggtgg gtttctgcag accgacatgg ttattctcgc catcggggta
    12061 aaacctgaga gcagccttgc cacaggagcc gggctcgccg tgggtaaacg gggaggcata
    12121 agtgtcaatg cctgtatgca gacaagtatc cctgacatct atgccgtggg ggatgcagtt
    12181 gaaacacctg accttgtctt tcaggaaccc gctaatgttc ctctggcagg gccagccaac
    12241 cgccagggac gcattgctgc tgataatatg ctcgatcgtc acagccttta ccatggcagt
    12301 caggggacat ccatttgtaa ggtattttcg ctgagtattg gcagtgtcgg ggcgaatgaa
    12361 aagcagctga aagcccatgg tacccgatac gagaaggttt acgtccatgc cgctgatcat
    12421 gccagttact acccgggggc aacaatgatc agtctgaaac tgctgttcag ccccgatacc
    12481 ggtaaaattc tcggggccca ggcgtcaggg aaaaaagggg ttgataaacg catcgatgtc
    12541 ctggctgtcg tacagcgcgc ggggcttacc gttaacgatc tcgagcatct ggagctgacc
    12601 tatgctccgc ctttcaacag tgcaagggac gtcgttaacc aggctggaat ggtggcggca
    12661 aatgttatca agggcgatac ggtcatctgt catgtccgcg atgtgttaca acgggaaccg
    12721 ggcagttatt gtttacttga tattcgttct cctgcagaac tgaaacaatt tggtgaatat
    12781 cctgacgcgc tatccatccc tcttgattcc ctgcgggaga atcttgataa gttgcctgaa
    12841 gataaagaga ttattatcgg gtgtcagagt ggtctgcggg ggcatgtggc ataccgtatg
    12901 cttcagtctc acggcttccg gacatataat ctgagtggtg gctttataac ttggcaggcg
    12961 gttacggaat caatgagtca ataagagatg aggtgccata cacatggcac ctcatcttcc
    13021 agattcattg aaaacgtatt atatggaata gaaatctaca aaaataatta acaccctgga
    13081 tggtacccta ttgcgataat gtggactgtt caaaaaacag gcttaccgta caataattct
    13141 ctatatccaa actgacccca atgttcctga atgtaaatct ccgtacaaag atatcgttga
    13201 gttttatata attcattgtt tttattgtgt tttgtttttt ttgttgtgct ggtttgtagt
    13261 tataaaaaac ccctgggaat ccaggggtag gtaaggaaaa caaatgatac tcagttagtg
    13321 ttgcacttta aacgggttga agtataactc gtattaaatg agatgttgaa aataagtgaa
    13381 aaccattgct ggttgcaata gggcagtatg cgtaaaaaat aaatttgtga tgcttttcaa
    13441 aaaaacaatt aatacatgaa tattacagct catatattac agtttataaa aaaactgtgt
    13501 tttatctatt acatagagaa aaattcttat cctgagctgt gaacttggtt gtttcgtttg
    13561 atatatatct ttgtaaatgt ggtttattat gcgggcattt tttattgagt attgaatcag
    13621 aataagtgat gcagctaaat ttatttgcgt aactatttca ttgtcaatat tctgactcaa
    13681 tctgacacct atcgtttctc ttggattccc cacttttgtg gtttttttat ccggaacgga
    13741 tagtgccagt tgttttttat cagcctcagg gtattttggg gcggttgcga acattgacgc
    13801 attccacaac taaaaaaatt tctcacagac tctgattctt gccccgaccc ttggtcggtt
    13861 tttttttgtc ctggcgaaac gtgaagctct gaaacagaaa atctccgggc agaaccttcc
    13921 atccgggggg agaaaacttt tcgttttgag ccgttcggtg ttcagaacgc acgaaaccga
    13981 tcgcacatcg ctttcgtgca tagttatgca acgccctgaa aacgattctg acgcgttttt
    14041 ctggttttgc ctggtgtttt cttgtctttt tgcgtttttt gcgccagaaa gcgactgagg
    14101 gcgttttaag gggggcgtac aacgggattt atggtaaatg gatcggtttt tcgggaagga
    14161 tcgacaggat ttgccgttgg gtgtagtgta agcgactgaa aaacaaacgc ccctgaaatt
    14221 cgtgtctatc cgccaagact ttcacgaaaa tacaggggcg atgtcggtcg ataacctgga
    14281 aggaatcgta acac
//
