LOCUS       NZ_CP058314             2348 bp    DNA     circular CON 18-AUG-2023
DEFINITION  Staphylococcus aureus strain BLR-DV plasmid pSTA-BLR-MRSA-DV-2,
            complete sequence.
ACCESSION   NZ_CP058314
VERSION     NZ_CP058314.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15361562
            Assembly: GCF_013389715.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Slizen,V., Akhremchuk,A.E. and Valentovich,L.N.
  TITLE     Characteristics of complete genome of methicilin resistant
            Staphylococcus aureus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Slizen,V., Akhremchuk,A.E. and Valentovich,L.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2020) Microbiology, Virology, Immunology,
            Belarusian State Medical University, Dzerzhinski Ave., 83, Minsk
            220116, Belarus
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JUN-2020
            Assembly Method :: Barapost v. v 3.14; Flye v. v. 2.7.1-b1590;
            SPAdes v. v. 3.13.2
            Assembly Name                     :: Staph_aur_4_v1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/18/2023 04:40:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,983
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 80
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 52 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 18 of 102
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058314.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BLR-DV"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pSTA-BLR-MRSA-DV-2"
                     /geo_loc_name="Belarus:Baranavichy"
                     /lat_lon="53.08 N 26.01 E"
                     /collection_date="2016-12-10"
                     /collected_by="Slizen V.V."
                     /note="MRSA strain"
     gene            1..477
                     /locus_tag="HW113_RS14900"
                     /old_locus_tag="HW113_14900"
     CDS             1..477
                     /locus_tag="HW113_RS14900"
                     /old_locus_tag="HW113_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000664727.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            1185..1919
                     /gene="erm(C)"
                     /locus_tag="HW113_RS14905"
                     /old_locus_tag="HW113_14905"
     ARG             1186..1916
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1186..1919
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          2..487
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaaagaaa gatatggaac agtctataaa ggctctcaga ggctcataga cgaagaaagt
       61 ggagaagtca tagaggtaga caagttatac cgtaaacaaa cgtctggtaa cttcgtaaag
      121 gcatatatag tgcaattaat aagtatgtta gatatgattg gcggaaaaaa acttaaaatc
      181 gttaactata tcctagataa tgtccactta agtaacaata caatgatagc tacaacaaga
      241 gaaatagcaa aagctacagg aacaagtcta caaacagtaa taacaacact taaaatctta
      301 gaagaaggaa atattataaa aagaaaaact ggagtattaa tgttaaaccc tgaactacta
      361 atgagaggcg acgaccaaaa acaaaaatac ctcttactcg aatttgggaa ctttgagcaa
      421 gaggcaaatg aaaaacaaga aaatgcatta tctgattatt attctttcaa ggactagtat
      481 aacataaaat cgtctacaaa tagacaaaaa acctgcacgc ttatgtgaat tagaaaaagc
      541 ttaatcgcat ttcatagatt gacctcccaa taactacgtg gtgttattgg gaggtcaatc
      601 tatttcattt gcgttaagct tttgatctac attaagcgtg caggtttaaa gtacataaaa
      661 aatataatga aaaaaagcat cattatacta acgttatacc aacattatac tctcattata
      721 ctaattgctt attccaattt cctattggtt ggaaccaaca ggcgttagtg tgttgttgag
      781 ttggtacttt catgggatta atcccatgaa acccccaacc aactcgccaa agctttggct
      841 aacacacacg ccattccaac caatagtttt ctcggcataa agccatgctc tgacgcttaa
      901 atgcactaat gccttaaaaa aacattaaag tctaacacac tagacttatt tacttcgtaa
      961 ttaagtcgtt aaaccgtgtg ctctacgacc aaaagtataa aacctttaag aactttcttt
     1021 tttcttgtaa aaaaagaaac tagataaatc tctcatatct tttattcaat aatcgcatca
     1081 gattgcagta taaatttaac gatcactcat catgttcata tttatcagag ctcgtgctat
     1141 aattatacta attttataag gaggaaaaaa taaagagggt tataatgaac gagaaaaata
     1201 taaaacacag tcaaaacttt attacttcaa aacataatat agataaaata atgacaaata
     1261 taagattaaa tgaacatgat aatatctttg aaatcggctc aggaaaaggg cattttaccc
     1321 ttgaattagt acagaggtgt aatttcgtaa ctgccattga aatagaccat aaattatgca
     1381 aaactacaga aaataaactt gttgatcacg ataatttcca agttttaaac aaggatatat
     1441 tgcagtttaa atttcctaaa aaccaatcct ataaaatatt tggtaatata ccttataaca
     1501 taagtacgga tataatacgc aaaattgttt ttgatagtat agctgatgag atttatttaa
     1561 tcgtggaata cgggtttgct aaaagattat taaatacaaa acgctcattg gcattatttt
     1621 taatggcaga agttgatatt tctatattaa gtatggttcc aagagaatat tttcatccta
     1681 aacctaaagt gaatagctca cttatcagat taaatagaaa aaaatcaaga atatcacaca
     1741 aagataaaca gaagtataat tatttcgtta tgaaatgggt taacaaagaa tacaagaaaa
     1801 tatttacaaa aaatcaattt aacaattcct taaaacatgc aggaattgac gatttaaaca
     1861 atattagctt tgaacaattc ttatctcttt tcaatagcta taaattattt aataagtaag
     1921 ttaagggatg cataaactgc atcccttaac ttgtttttcg tgtacctatt ttttgtgaat
     1981 cgattatgtc ttttgcgcat tcacttcttt tctatataaa gcgtcggaaa agcagcaaaa
     2041 agtttccttt ttgctgttgg agcatggggg ttcagggggt gcagtatctg acgtcaatgc
     2101 cgagcgaaag cgagccgaag ggtagcattt acgttagata accccctgat atgctccgac
     2161 gctttatata gaaaagaaga ttcaactagg taaaatctta atataggttg agatgataag
     2221 gtttataagg aatttgtttg ttctaatttt tcactcattt tgttctaatt tcttttaaca
     2281 aatgttcttt tttttttaga acagttatga tatagttaga atagtttaaa ataaggagtg
     2341 agaaaaag
//
