LOCUS       NZ_CP058313             2979 bp    DNA     circular CON 18-AUG-2023
DEFINITION  Staphylococcus aureus strain BLR-DV plasmid pSTA-BLR-MRSA-DV-1,
            complete sequence.
ACCESSION   NZ_CP058313
VERSION     NZ_CP058313.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15361562
            Assembly: GCF_013389715.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Slizen,V., Akhremchuk,A.E. and Valentovich,L.N.
  TITLE     Characteristics of complete genome of methicilin resistant
            Staphylococcus aureus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Slizen,V., Akhremchuk,A.E. and Valentovich,L.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2020) Microbiology, Virology, Immunology,
            Belarusian State Medical University, Dzerzhinski Ave., 83, Minsk
            220116, Belarus
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JUN-2020
            Assembly Method :: Barapost v. v 3.14; Flye v. v. 2.7.1-b1590;
            SPAdes v. v. 3.13.2
            Assembly Name                     :: Staph_aur_4_v1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/18/2023 04:40:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,983
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 80
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 52 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 18 of 102
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058313.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BLR-DV"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pSTA-BLR-MRSA-DV-1"
                     /geo_loc_name="Belarus:Baranavichy"
                     /lat_lon="53.08 N 26.01 E"
                     /collection_date="2016-12-10"
                     /collected_by="Slizen V.V."
                     /note="MRSA strain"
     gene            1..996
                     /locus_tag="HW113_RS14890"
                     /old_locus_tag="HW113_14890"
     CDS             1..996
                     /locus_tag="HW113_RS14890"
                     /old_locus_tag="HW113_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199895.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAYNVKQCGNVLEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EEVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQKALQVDYRPVANIKAI
                     KPNQKGDKDIESAIKETSKYSVKSSDFLTDNDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKITDEEAKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            complement(1947..2528)
                     /locus_tag="HW113_RS14895"
                     /old_locus_tag="HW113_14895"
     CDS             complement(1947..2528)
                     /locus_tag="HW113_RS14895"
                     /old_locus_tag="HW113_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991701.1"
                     /translation="MNAIQKSVSLRKVLVISLSFLTILSMVLNFNFKEAQAQNKNNIAD
                     KNIETLNEKEIEKELDYIYGKIIVLDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKNSSISPRAVGGNYKNSNDCFYSEVLNSYGELLTGNIIAAVFDDVKAKNIKSAAKKL
                     ARIGIKGNLAGIAATMVSKDVQCTLKYGWF"
                     /name="hypothetical protein"
     Typing          1052..1199
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.329"
                     /coverage="100.0"
                     /evalue="2.78e-70"
     Typing          2..996
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.687"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcaatata atactactag aagtataacc gaaaatcaag ataataaaac gttaaaagat
       61 atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga aaatagataa tgtaagctat
      121 gcagatatac ttgaaatttt aaaaataaaa aaggcttata acgtaaaaca atgtggtaac
      181 gtcttagaat tcaagccgac cgatgaaggt tatttgaaat tacataagac gtggttttgt
      241 aaatcaaagc tatgtccagt ttgtaattgg aggcgtgcta tgaaaaatag ttatcaagct
      301 caaaaagtga ttgaagaagt aattaaggaa aagccaaaag cacgttggtt gtttttaaca
      361 ctttcaacca aaaatgcgat agatggagat actttagaac aaagtttgaa gcatctaact
      421 aaagcatttg ataggttgag tagatataaa aaggttaaac aaaatcttgt tggatttatg
      481 cgttcaacag aagttaccgt taataaaaat gatggtagtt ataatcagca tatgcacgtt
      541 ttgttatgtg ttgaaaatgc atattttaga aaaaaagaga attatataac tcaagaagaa
      601 tgggttaatt tatggcaaaa agcattacaa gttgattatc gacctgttgc aaatattaaa
      661 gcaattaaac ctaatcaaaa aggcgataaa gatattgaat cggcaatcaa agagacatca
      721 aaatattcgg ttaagtcatc tgatttttta actgacaatg acgaaaaaaa tcaagaaatt
      781 gtaagtgatt tagaaaaagg tttatatcga aaacgtatgt taagttatgg tggattgctt
      841 aaacaaaagc ataaaatttt aaacttagac gatgctgaag atggtaattt aattaataca
      901 agtgatgaag ataaaataac agatgaagaa gcaaaagcac attcaattac ggcaatttgg
      961 aattttgaaa aacaaaatta ttatttaaga cattagtgat gactgatgtc ttttttattg
     1021 attttttgta aaaaagtact gtcttatttt tgtgacaaat gctgtatgta gtgtcacaaa
     1081 actgtgacac tacagctttt tatgatatca ctttaaaatt acttaaaacc cttggaatgt
     1141 ctggctttgc cagacctatc atttttgaat gatagcaaat tctccttatg ctcttacgga
     1201 gttcttagag aaaaattaaa aattctcgat ttttgataaa aagcgccctg caggaattta
     1261 gaaaaacatg ggggaagttt taaaggattt tatgctaatt tttaatttgc atgtaaatgg
     1321 gcagtgtccg aaaaatcgac actgggtttg atcgaatttt tgattataaa aaatttttga
     1381 ttataaaatc agaatatatt tatttggctc atatttgcgt tttaagaaac aaataagata
     1441 atacaggtat aaaactatat atttttaaag ttcgttctaa ataccccctt aaaatgcaaa
     1501 ataagtatat tcgattatta cacgtaaatt ttggttttag ctcgacgcat ttttaagtat
     1561 gtaaatgcgc ctcttggata acgaaaaata aaaaatctta attaaacaga gtttaactaa
     1621 gatagtcacg aattttatta ggtttaaaaa ttatgattac ttcagatgtt tagaattaag
     1681 gtgttgatta tagtgtgaat gaaagcaagg gataataatt agtaaatcag tgcacaatta
     1741 atgttttatt taagggatag ttacacgcat aatcaaacat gcatttatgc cgagaaaatt
     1801 tattgatgtt gagaagaacc cttaactaaa cttgaagacg aatgtcggca tagcgtgagc
     1861 tattaagccg acgattcgac aagtttaggg attgttaagg gttccgaggc tcaacgtcaa
     1921 taaagcaatt ggaataaatt atatatttag aaccatccgt atttcagagt acattgtaca
     1981 tcctttgaaa ccatagtagc tgcaattcca gctaaatttc cttttatgcc aattctggct
     2041 aatttttttg ctgcactctt tatgtttttg gcttttacat catcaaaaac tgctgcgatt
     2101 atattaccgg ttaaaagttc tccatatgag tttaaaactt cagaatagaa acagtcatta
     2161 ctatttttgt agtttccacc aactgctcta ggtgatattg agctattttt ctttttattc
     2221 tctatgtcgt tctttagttt ttggaaacct tcaggaacat atccatatct agatttggca
     2281 ttttctagat taacatcttt agcggttccg tctttatcta aaacaataat tttgccatat
     2341 atgtagtcta gttctttttc gatttctttt tcgtttaaag tttcaatgtt tttatctgcg
     2401 atgttgtttt tgttttgggc ttgagcttct ttaaaattaa aattcaagac cattgaaagt
     2461 atagttaaaa aacttaaaga tattactaaa acttttctta gtgatacact tttttgaatt
     2521 gcattcatta taaatgcccc cttgttttgt tatttaaatg aatccaatat aatattaaca
     2581 tataaaaaca aaggagttta atatgaataa gtttatttat tttttaattg ttttattgtc
     2641 cgtattttta atatttaatt ttgttaattc gattttattt aataatcctt ttgacttttt
     2701 agaaagcttt atttattcgt tggttttcac attgatttat ttaggtttag agggctataa
     2761 agagaaaaag aaatagattg tttttgaaac gagttgaaaa cgagtttctt cttgtcttga
     2821 tactatatag aaataactcg attttatata tatagctgta actgttgata ttacagcgtt
     2881 taaacgtgtt tttgtgcgtg aaaggaaaat ttgacaataa aaaaccccag ttatattatt
     2941 aaggtgtcga atcttaaata atactggggg tctttttat
//
