LOCUS       NZ_CP058229             5595 bp    DNA     circular CON 21-FEB-2024
DEFINITION  Klebsiella pneumoniae strain KPCZA02 plasmid pKPCZA02_3, complete
            sequence.
ACCESSION   NZ_CP058229
VERSION     NZ_CP058229.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15343240
            Assembly: GCF_018831545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Xu,M., Zhao,J., Xu,L., Yang,Q., Xu,H., Kong,H., Zhou,J. and Fu,Y.
  TITLE     Emergence of transferable ceftazidime-avibactam resistance in
            KPC-producing Klebsiella pneumoniae due to a novel CMY AmpC
            beta-lactamase in China
  JOURNAL   Clin Microbiol Infect 28 (1), 136 (2022)
   PUBMED   34044150
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Fu,Y., Xu,M. and Zhou,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2020) Department of Respiratory Diseases, The
            First Affiliated Hospital, College of Medicine, Zhejiang University,
            79 Qinchun RD, Hangzhou 310003, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 8.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/21/2024 03:03:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,675
            CDSs (total)                      :: 5,553
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 50 of 134
            Pseudo Genes (incomplete)         :: 82 of 134
            Pseudo Genes (internal stop)      :: 18 of 134
            Pseudo Genes (multiple problems)  :: 16 of 134
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058229.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPCZA02"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPCZA02_3"
                     /geo_loc_name="China"
                     /collection_date="2015-06-02"
     gene            join(5201..5595,1..22)
                     /locus_tag="HWQ20_RS27600"
     CDS             join(5201..5595,1..22)
                     /locus_tag="HWQ20_RS27600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_215824897.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQRDGAIVLQGKPEEVRELID
                     ASPYAKKYTSGVLSFAEQDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLE
                     LNFLVPNTELLTGRRLQPYYDRADRPRMMRGRPW"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            293..802
                     /locus_tag="HWQ20_RS27605"
     CDS             293..802
                     /locus_tag="HWQ20_RS27605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            809..1021
                     /locus_tag="HWQ20_RS27610"
                     /old_locus_tag="HWQ20_27610"
     CDS             809..1021
                     /locus_tag="HWQ20_RS27610"
                     /old_locus_tag="HWQ20_27610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1397..1525)
                     /locus_tag="HWQ20_RS28550"
     CDS             complement(1397..1525)
                     /locus_tag="HWQ20_RS28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            1688..1912
                     /locus_tag="HWQ20_RS27615"
                     /old_locus_tag="HWQ20_27615"
     CDS             1688..1912
                     /locus_tag="HWQ20_RS27615"
                     /old_locus_tag="HWQ20_27615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1896..2162
                     /locus_tag="HWQ20_RS27620"
                     /old_locus_tag="HWQ20_27620"
     CDS             1896..2162
                     /locus_tag="HWQ20_RS27620"
                     /old_locus_tag="HWQ20_27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2232..2582)
                     /locus_tag="HWQ20_RS27625"
                     /old_locus_tag="HWQ20_27625"
     CDS             complement(2232..2582)
                     /locus_tag="HWQ20_RS27625"
                     /old_locus_tag="HWQ20_27625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2648..3193)
                     /locus_tag="HWQ20_RS27630"
                     /old_locus_tag="HWQ20_27630"
     CDS             complement(2648..3193)
                     /locus_tag="HWQ20_RS27630"
                     /old_locus_tag="HWQ20_27630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(3219..3545)
                     /locus_tag="HWQ20_RS27635"
                     /old_locus_tag="HWQ20_27635"
     CDS             complement(3219..3545)
                     /locus_tag="HWQ20_RS27635"
                     /old_locus_tag="HWQ20_27635"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3903..4061)
                     /locus_tag="HWQ20_RS28625"
     CDS             complement(3903..4061)
                     /locus_tag="HWQ20_RS28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4701..4877
                     /locus_tag="HWQ20_RS27640"
                     /old_locus_tag="HWQ20_27640"
     CDS             4701..4877
                     /locus_tag="HWQ20_RS27640"
                     /old_locus_tag="HWQ20_27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4867..5211
                     /locus_tag="HWQ20_RS27645"
                     /old_locus_tag="HWQ20_27645"
     CDS             4867..5211
                     /locus_tag="HWQ20_RS27645"
                     /old_locus_tag="HWQ20_27645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4536..4615
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3953..4350)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2..1137
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.736"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5202..5593
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="3.47e-78"
ORIGIN
        1 gatgcgtggc agaccgtggt gaacgggcga ctggggctgc acgacccgaa cgcaccggag
       61 aaccggcgcg cgctggtgac gccatcttcg ttacccaaaa cgaagcagga agccgcagag
      121 gctattacgc ggggcttact ggccctcgcc tcgtcagggg agcttaaaac gcgtcaggac
      181 gtcactgagg cgctggaaag cgcaggtttt gaggtcgtgc gcaccacaaa gagcagcatc
      241 agcattgccg acccggacgg agggcgaaac atccgactta agggagccat ctatgaacag
      301 tcttttaacg ctggcgaagg acttagagca gaaatcgaaa gcgcagcagc agagtaccgg
      361 cgaaatgctg aaagccgcat tcagcgagca cgagaagtct gtcagagcgg aactgagcga
      421 aagcgggaag agaatcagcg ccgccatccg cgcccacgag cagggtatga gcgcggccat
      481 gcagtcgaac cgcctgagcg tgatgcgtat ggtcgggcgg acatggctga ccatcacgat
      541 ggtgtcagga ctgctgttcg ccagcctgag cggggtgttg tggtatcagg ggagcttgat
      601 agcgtcaaat ctggccgaaa tcgaccggca gaacgcagcg ctgtcgaagc tgaacgcgaa
      661 gacctggggc gtgacgtacc tggaagacag caacggacgt tttctggtgc tgccgaaggg
      721 gacggccgta gacaggacgc agagctggac ggtcgggaac gggcggtcga agcagaacgc
      781 gctgagactg gtgaaggagt aagtgccgat gaccgagctg gaaaaacagt tgctgagcgc
      841 attagagcag ctacagcagg actactcgaa aaggctggac gagtgggaga gcgccttcgg
      901 ggaatggcgg aaaatgtcag ggcttatgca acgggagaac gcggcgctga gcgagcgcgt
      961 gacgcgcttg agtcagcagg tggaacgctt gagcgggcaa ctgcagcgtt tgagccggta
     1021 gtgcagcgtc atgagatggc cgtggccgca gaacgggcgc acgagcaacg gcagcacgaa
     1081 aaggaactga cggaagcgcg ttcccggcag cgaagctatg acggtccgtc gctgggctga
     1141 aatgcaggaa aacagggttc tcatcgggca tttacgctga aaccggatgt cattcacctg
     1201 gctgcatggc tatgcagcca ggtaaaattt atctgtcgcg aacggggcgg tttatcgggt
     1261 ggtttgttgc cggttttacc tgactgccac cccgttcgcg gcgaacccgt ccggggcggt
     1321 gcgggcaacg ggtgttatgt ttaatcagcg tgaacggcag atacaaaaaa gccccggagg
     1381 gcttttaact actgacttat ttttttgaag atttgctggg tttcaatttg tcagtatttc
     1441 ccggcgtttt tttattgtca cgctggcgtt tatcaggttc ccctgttgaa tcggcaattc
     1501 gtttcggacc cggcttaaga cccataatcg ttatcctttc aatagttaag aggaaagccc
     1561 tcaagctaga tatattgacc gcgttacgga ttatcaagag gggctaacaa gattattttt
     1621 tgttctttta ggttgattgt ggtactgttg atatacatgt atagcaaatg gtgttcggga
     1681 ggtttatatg cttgctatcc ggttatctga tgagattgag tcccgtctgg actcgctggc
     1741 gaagcaaacc ggcagaacaa agacgtttta tgcgcgggaa gcaatactgg cgcatctgga
     1801 agacctggag gattattatc tttcagcaga aactgctgca cgcgttcgcc gtggtgatga
     1861 agcagtgcat tcgtctgaag acgtgaggaa gtcacttggt ctggacgatt aactattccg
     1921 atcgggcgct caaatcgtta cgcaagatgg acaaacagaa cgcacgacgg attgtggatt
     1981 ttatgagttt acgcattgca gttgctgccg atcctcgcca gtcagggaag ccgctcaaag
     2041 gtgagctggg cgagttctgg cgctatcggg tgggagatta tcgcgttctg tgtgagatcc
     2101 gagatgacga gcttgttatc cttgccgcca cgattggaca tcgccgcgaa gtttacgact
     2161 gaagcccgct atgcgggctt ttttgatgcc gacaacaggt attatggtaa atactcgact
     2221 aactacgtgt tctactgttt gtcggtttta gttaaccagc aggttgtctt aacgataatg
     2281 tagccgttat ttttaactaa taaggtcata ttgttaacgt caggcataat aaaagacatt
     2341 atggcctcag agttatcatc agaagcagct ctctgcatta ctacttcgat tcccttttct
     2401 ttgaatctat aagtatccag ttgcctatta aacttagaaa gacctacaac catacttagt
     2461 ttgttatctt taacagtaag agatgcttcc tgagtaccat tacatcggta gtaagcggtg
     2521 taaccgtcag caaaagatcg accggggaat ataaatgcta ggaacgcaat taaccaatac
     2581 atagttgaga atcttggtat tacacatttc actatcctct ctttcatttt tcgttcacca
     2641 tactttgtta tctgattttt aatttttcta ttttataaac caagtctcta ccaaaaaggc
     2701 gaaaattatc ctcaaataat aataattgtt taagaacatc catgaaagca gctgcttcac
     2761 cctcagttat gggagctact ctatataaca tataacctga gttaagggtt tcttttatac
     2821 tttcttcaat gctcattata aatattagtg gattatcatt tttcttcccc catgggtttc
     2881 taattaatcc ttcgatgtac tcaggtcctt tgcgtcctat tgcctgcgca atatttttta
     2941 tttcatctgc gtgagggctt tgttgtacag ctttgtttat gcctgcttga gtgattttca
     3001 gagactctac accgtgtatg ttgtatgaga taaaatgtaa taaattaaga tgcaatgcaa
     3061 ttaatccatt tttgtttaca tctgagtatt tggataaaaa acgtaatgaa gtgttaactc
     3121 tcccttgaag cctaacaatt gggagtgctg ccttgtatat ttccttctcc gttaaatacc
     3181 gtttaaaaaa cataattctc cgcctccgta tagcgtgcct attttatttc gtcgtaaaac
     3241 ttagctctga aatattcctt tagaaccatc atgtcattaa taattgaatc tatctcatcc
     3301 attttttctt ggtattcttt taatggtgaa gggagcggta actcaatatt tgaaaagtga
     3361 ttggcgagat agggatcaag gtttctcatc cattcaggct tttcgccaat gcgttcttca
     3421 actgaaagtg aacctgataa tctctggtta ataagatcac ttagactcat tttggtttta
     3481 ttcgaaagcc gttgtaagga ttgctttaat cctatatcta gcatgatatt tgttctttct
     3541 ctcattaccc ccccttgcta ttgcgttttt tgttgcgcac aaagctacca ttgcgcatta
     3601 agaaacgcaa taaaaaagga gggagggagt ggtttgtaca tgatgtgcag tgcgctgagg
     3661 gcatcagtgg cgggtgtttt tccgtgggat ggatggagct ccggggataa cagaactgac
     3721 gatcatttaa aggatcttct tgagatcctt tttttctgcg cgtaatctct tgcactgtaa
     3781 acgaaaaaac cgcctgggga ggcggtttga tcgaaggtta agtcagttgg ggaactgctt
     3841 aaccgggtaa ctgggtatac gagaccgcag ataccaaaac tgttctttca gtgtagccgc
     3901 agttaggcac ccacttcaag acctctcaat atctctctcg tatatatcca gttaccaatg
     3961 gctgctgcca gtggcgtttt tgcgtgtccg tccgggttgg actcaagatg atagttaccg
     4021 gaaagggcgc agcagtcggg ctgaacgggg ggttcgtgca tacagcccag cttggagcga
     4081 acgacctaaa ccgagccgag ataccagcag cgtgagctat gagaaagcgc cacgcttccc
     4141 gaaagggaaa aaggcggaac aggtatccgg taaacggcag ggtcggaaca ggagagcgca
     4201 cgagggagcc gccggagaga aatcaccggc atctttaagt cctgtcgggt ttcgtcctcc
     4261 tctgacttga gcgtccattt ctgtgatgct cgtcagggga ggcggagcct atggaaaaac
     4321 ggcaacgccg catccttttc tccggccctt tctgatttga gcgcctttgc tttatcccgt
     4381 tatccgtgag tcggtcacgc ccgccgcagc cgaacggcag gagcgcagct accgagtgag
     4441 cgaggaggcg tcatttcatc tgtccgtgca tggtattgcg gacgctcctg agccacatat
     4501 cacacctgcc acatgaggca ctttcatgaa tcgtgctctc atgccaacat agtaagccag
     4561 tatacacagc gtaaacgctg tgactggttc agggctgcgc cccgaaaccc gctaacacca
     4621 ctgacgcgct ggcgcttgtc caacgccgga gcgacccgga aaacttttcc gtgccgtccg
     4681 gcgtttgcga aggagggata atgcagaagc cgaagaagaa gccgctcgtc gtaaaaaaaa
     4741 gatcctggca tccggcagtg atggcaaatc tgatgcgtca ggcacaggag gaagattacc
     4801 gccgggagca gatggcagag cttgagcgtt cagcccggtc acattttgcg gatatcggag
     4861 gagaggatgg cggttaagcg tacccgtttt ttaggtatcc gggtgaccga tggggagtac
     4921 cagcagctgc tggagcgctg caatggaagg caactggcgg tgtggatgcg ggaaacctgt
     4981 ctcgatacgc gcccggcgcg gtcgttgcgg ctgccgtcca tcgatccggt tttgctgcgc
     5041 cagcttgccg ggatggggaa caacctcaac cagatcgccc ggaaaattaa cggcggccag
     5101 tggtcaggcg ctgaccgggt tcaggtggtg gccgcgctga tggccattga tgccgaactc
     5161 gagcggctgc ggcatgccgt gctggaaaag ggggcagacg atgatcgtta agtttcatcc
     5221 gcgagggcga ggcggcggtg ccgggccggt ggattatctg ctggggaaag accgccagcg
     5281 cgacggggcc atcgtcctgc agggaaagcc ggaggaggtc cgggagctta tcgacgcctc
     5341 gccctacgcc aaaaagtaca cctccggcgt cctgtctttt gctgaacagg atttaccgcc
     5401 cggccagcgt gaaaagctga tggcgagctt cgagcgggtt ctgatgcccg gactcgataa
     5461 agaccagtac agcgtgctgt gggttgaaca ccgggacaag gggcggctgg aactgaattt
     5521 tctggtaccg aacacggaac tgctgaccgg caggcgtctc cagccgtact acgaccgggc
     5581 agaccgtccg cgcat
//
