LOCUS       NZ_CP058227            10059 bp    DNA     circular CON 21-FEB-2024
DEFINITION  Klebsiella pneumoniae strain KPCZA02 plasmid pKPCZA02_1, complete
            sequence.
ACCESSION   NZ_CP058227
VERSION     NZ_CP058227.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15343240
            Assembly: GCF_018831545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Xu,M., Zhao,J., Xu,L., Yang,Q., Xu,H., Kong,H., Zhou,J. and Fu,Y.
  TITLE     Emergence of transferable ceftazidime-avibactam resistance in
            KPC-producing Klebsiella pneumoniae due to a novel CMY AmpC
            beta-lactamase in China
  JOURNAL   Clin Microbiol Infect 28 (1), 136 (2022)
   PUBMED   34044150
REFERENCE   2  (bases 1 to 10059)
  AUTHORS   Fu,Y., Xu,M. and Zhou,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2020) Department of Respiratory Diseases, The
            First Affiliated Hospital, College of Medicine, Zhejiang University,
            79 Qinchun RD, Hangzhou 310003, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 8.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/21/2024 03:03:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,675
            CDSs (total)                      :: 5,553
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 50 of 134
            Pseudo Genes (incomplete)         :: 82 of 134
            Pseudo Genes (internal stop)      :: 18 of 134
            Pseudo Genes (multiple problems)  :: 16 of 134
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058227.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10059
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPCZA02"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPCZA02_1"
                     /geo_loc_name="China"
                     /collection_date="2015-06-02"
     gene            join(8722..10059,1..30)
                     /locus_tag="HWQ20_RS26520"
                     /old_locus_tag="HWQ20_26515"
     CDS             join(8722..10059,1..30)
                     /locus_tag="HWQ20_RS26520"
                     /old_locus_tag="HWQ20_26515"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_228745764.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKRRMQKIS"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            108..347
                     /locus_tag="HWQ20_RS28375"
     CDS             108..347
                     /locus_tag="HWQ20_RS28375"
                     /inference="COORDINATES: protein motif:HMM:NF020569.4"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="WP_201286985.1"
                     /translation="MKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIYEWDSQHGELE
                     GYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            357..614
                     /locus_tag="HWQ20_RS26525"
                     /old_locus_tag="HWQ20_26520"
     CDS             357..614
                     /locus_tag="HWQ20_RS26525"
                     /old_locus_tag="HWQ20_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            699..848
                     /locus_tag="HWQ20_RS26530"
                     /old_locus_tag="HWQ20_26525"
     CDS             699..848
                     /locus_tag="HWQ20_RS26530"
                     /old_locus_tag="HWQ20_26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            2332..4296
                     /locus_tag="HWQ20_RS26535"
                     /old_locus_tag="HWQ20_26530"
     CDS             2332..4296
                     /locus_tag="HWQ20_RS26535"
                     /old_locus_tag="HWQ20_26530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4296..5027
                     /gene="mobC"
                     /locus_tag="HWQ20_RS26540"
                     /old_locus_tag="HWQ20_26535"
     CDS             4296..5027
                     /gene="mobC"
                     /locus_tag="HWQ20_RS26540"
                     /old_locus_tag="HWQ20_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5034..5564
                     /locus_tag="HWQ20_RS26545"
                     /old_locus_tag="HWQ20_26540"
     CDS             5034..5564
                     /locus_tag="HWQ20_RS26545"
                     /old_locus_tag="HWQ20_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5591..5770)
                     /locus_tag="HWQ20_RS26550"
                     /old_locus_tag="HWQ20_26545"
     CDS             complement(5591..5770)
                     /locus_tag="HWQ20_RS26550"
                     /old_locus_tag="HWQ20_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5796..6224)
                     /locus_tag="HWQ20_RS26555"
                     /old_locus_tag="HWQ20_26550"
     CDS             complement(5796..6224)
                     /locus_tag="HWQ20_RS26555"
                     /old_locus_tag="HWQ20_26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(6388..6702)
                     /locus_tag="HWQ20_RS26560"
                     /old_locus_tag="HWQ20_26555"
     CDS             complement(6388..6702)
                     /locus_tag="HWQ20_RS26560"
                     /old_locus_tag="HWQ20_26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6690..6968)
                     /locus_tag="HWQ20_RS26565"
                     /old_locus_tag="HWQ20_26560"
     CDS             complement(6690..6968)
                     /locus_tag="HWQ20_RS26565"
                     /old_locus_tag="HWQ20_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(7143..7508)
                     /locus_tag="HWQ20_RS28380"
     CDS             complement(7143..7508)
                     /locus_tag="HWQ20_RS28380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(7505..7876)
                     /gene="tolA"
                     /locus_tag="HWQ20_RS26575"
                     /old_locus_tag="HWQ20_26570"
     CDS             complement(7505..7876)
                     /gene="tolA"
                     /locus_tag="HWQ20_RS26575"
                     /old_locus_tag="HWQ20_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(8150..8395)
                     /locus_tag="HWQ20_RS26580"
                     /old_locus_tag="HWQ20_26575"
     CDS             complement(8150..8395)
                     /locus_tag="HWQ20_RS26580"
                     /old_locus_tag="HWQ20_26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             3722..10059
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(952..1530)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4297..5024
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 aaaaagcgaa ggatgcagaa aataagttaa atgaggaaaa gaacaagcct cgcaagggga
       61 ctaaagacta cggccatgat tatcatccag tacctaaaac agaagacata aagggacttg
      121 gagagttaaa gcgaggtgat cctaaaacac ctaaacaagg tggtggtggt aagcgggccc
      181 gatggtatgg cgataaaaaa cgtaaaattt atgaatggga ttcccagcac ggtgagcttg
      241 aagggtatcg tgccagtgat ggcgaacacc taggcgcatt tgatcccaaa acgggtaagc
      301 aggttaaagg gccagatcca aaacggaaca ttaaaaaata tctttgagag gtaaatatgg
      361 ggcttaaatt acgtttgaca tggtttgata agaaaactga agagtttaaa ggggaagagt
      421 attctaaaga ctttggtgac gatggttctg tcattgaaag cttgggaatg cctttaaagg
      481 ataatattaa taatggtgag tttgatttga agaaagaatg ggttccttta ctacagccat
      541 tttttaaaaa tcaaataaaa cctgatgtgg ataattgtta tatagctttt gattatcggg
      601 acggtaactg gtaacaaaaa attatcaagg atgatttgaa atactgactt ccggtgttca
      661 gggatgaatg ccggagtctt taaatacagg gaaagtatat gaaaaaggta aagactattt
      721 ttctttttat attgattgtt gctggctttc ttctggccgc atgtcaggca aattatatcc
      781 gggatgttca gggcggaacg gtggcaccat cgtcctcctc tgaactgacg gggatcgcgg
      841 ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatcttttg
      901 ccctgtaaac gaaaaaacca cctggggagg tggtttgatc gaaggttaag tcagttgggg
      961 aactgcttaa ccgtggtaac tggctttcgc agagcacagc aaccaaatct gtccttccag
     1021 tgtagccgga ctttggcgca cacttcaaga gcaaccgcgt gtttagctaa acaaatcctc
     1081 tgcgaactcc cagttaccaa tggctgctgc cagtggcgtt ttgcgtgctt tcccgggttg
     1141 gactcaagtg aacagttacc ggataaggcg cagcagtcgg gctgaacggg gagttcttgc
     1201 ttacagccca gcttggagcg aacgacctac accgagccga gataccagtg tgtgagctat
     1261 gagaaagcgc cacacttccc gcaagggaga acggcggaca ggtatccggt aaacggcagg
     1321 gtcggaacag gagagcgcaa gagggagcga cctaccggaa acggtgggga tctttaagtc
     1381 ctgtcgggtt tcgcccgtac tgtcagattc atggttgagc ctcacggctc ccacagatgc
     1441 accgaaaaag cgtctgttta tgtgaactct ggcaggaggg cggagcctat ggaaaaacgc
     1501 caccggcgcg gccctgccgt tttgcctcac atgttagtcc cctgcttatc cacggaatct
     1561 gtgggtaact ttgtatgtgt ccgcagcgcc cgccgcagtc tcacgcccgg agcgtagcga
     1621 ccgagtgagc gaggaggcgc aatttccccg gcacctgcaa gacggtcacc ggacgcgctg
     1681 atgtgggata ttgtcccctg attaagtctt tctattccct gacgatcaat tatcaggata
     1741 ggtgaaccat gtcggttttt cgctgaacat tttacgttaa cctatctctg ttgctttttt
     1801 ctccttgcgg agaaatgcaa gtcatccccg ctggggcgtg gctgacttca ggggaaaaga
     1861 ccttgcttac gctggctgga cgccaccgac gcacggtctt ttcccctctt tctggctctg
     1921 tccggctgga cgccggccac gccagaaatt cacccggccc ccgtcaggct cacgcctgac
     1981 cacccacaaa aatccacggt gctggacgca cctgacgaag cagcaaagct gctcctgatt
     2041 ttgcgggtgg tcagccgctc gctatggcga actctgacgg gggccggaac accggaaaag
     2101 accgcactgg ggtgccgcct tttcctctca cacgctgggg cgtgctcgtt gttcctccgg
     2161 ggctgcgcct tttccgcatg gggcggaaag aggctggcct ccgcggtgaa agctgaacgc
     2221 aatcaccacg gacgtgaccg ccgccgccat gccttggggc atgtccgcgg cttgtgcagc
     2281 gggggctgcg catcacgccg gtaaacccgt cgtctgaagg agagagattg catgtttaat
     2341 acggattcgc ttgcctggca gtggcatatc ccggagctgg cccggcagat ttacccgtgg
     2401 gtcagctggc tgtaccggga gtattacagt ctggccggtt tttttctggt tatcggcctg
     2461 tttttcggtc tggctgaact ctgggccggc cgaatgcgtt ttgtggggct gagccataag
     2521 gcgcggcggg agtatgccga tcgctataag cgcgataata accggactgt gccattttcg
     2581 gtgctggcct cgctggattc acccgggcgg atggtcaggg cacggcgaca tgtctggaac
     2641 tacgcgtact ggctgaccgg cgctgccggt ctggcccttc tgggggggct ggttgtcgcc
     2701 gtgtttatcg agagctgggg agaaaccgtc ctgcgcccgg acctctatgc gaacgcagat
     2761 tattacctgc tgcagccggc taacctgttc gggatagcct ggtgtgccgg agggcttgtc
     2821 gccggcgtcc ttctggcgcg gctgctggcg ttcctgctgc ttgatggtca ttttgcggcc
     2881 gctgacgagg cggtgaacgc gaaactgaat caggagagcc ggcgcagcag ccagcggacc
     2941 ggagaaatga cagacgtccg ccacctgcat ttcggggagc cggtaccggt taacgcgctg
     3001 gccgattttt ccacggagca ggcccggaaa cagcaggcgg tgtttctggg taaggatgag
     3061 cagggccagc cggtgctggt ccctcgtgat acctggcgca agaccaatat tcagatcctc
     3121 ggcctgcccg ggagcggcaa gagcgcgatg gccaccaacg cgctcattcg ctgcgtacgc
     3181 gatttcgggg acgctgtggt gtacttcgac cccaaagggg acgcgtgggc gccgcacgtc
     3241 ttccgggcgc actgcccgga cttcaccctg ctggatctgc ggccggggaa accggcgcag
     3301 ctgaatctgt tccgcgatct cgaccagtac gccctgaaga atctgctggt tgccgggttt
     3361 aacctgagtg agaccggcga cgtggcggac cactaccgca tcagcgagca gaaagcggcg
     3421 aagctgattg cggagcagtt cccgcagggg gcaaacattc agcaggtgct ggcggcggcg
     3481 tacgcgttgc cggaggccct gaaaaaggac gtgaaggggc tgatcaccaa gctggagaac
     3541 gtggccgacc tgagcgtcct gcagacggac agcggtatcg atgtggcggg gataatcaac
     3601 ggcggcggct gcctgtacgt catcggctcg atggacgatg aggcggtgat ccgggtgcag
     3661 aagatgctgt ttgcccgctg cgcgcaaatc atcattgcgc gggatgagtt ccggaggtgg
     3721 ccgcacgcga gcatcatgct cgatgagatt aaatatctgc tgtcgaagta cgtgctgaac
     3781 gcgctgggga cgctgcgcag ccgggactgc aacctgctgc tcgcgcacca gtcgctgggc
     3841 gacttcggtc agtgcgggca ggatctgccc gctgattttg tgaaaaccac ggtactggat
     3901 aacacgccga tccgctggtt ctatcgcgcg gccagccagg agtccgcgca gtgggcggcg
     3961 ggccagaccg gggagatccg ggtggacgtg gagcgccgcc gggccagccg ggaggcgggg
     4021 aacgtggagc acatcagcgg cgacagcttc atccagaagg aggcgcggcc gctgtttgac
     4081 gtgaacaccc tgcaacacct gcctgacggg ttcgcggtga tgaccgggct gggcgtggcg
     4141 cggctggcgt tcagcagccc gctgcgggtt gagcgccggg aaatttcgct gaaaaccttc
     4201 ccggtgctgg cgaaaacgga cccgctggcg gagtacctgc ctccggccga cggcggcgga
     4261 ccaaaagcgg atgatgattt tgcggggctt tactgatgac actggagcag tacaacgtca
     4321 gccacgcgaa gcggcaggcg cggaacgcgg agaaaacacg gctgaccctg cgctggctgc
     4381 gggaagagct gtgttccacg gcggagctgg ttgcgcgacg gctggggatt gcggcggtac
     4441 agccggttta ccgcttcctg gacagtctgg tggcgaaagg gctgctggtc cgggcgaaat
     4501 acccggttga cggccgtcag gtcagcgtgt gggggctgac gccgcacggg gtggcgttct
     4561 cgttcgacga ggacgagccg ctgacggacg ttattccgtt tcagccgtca agggtgtcgg
     4621 cggcgcagct gccgcaccgg ctggccgtgc agtccctgcg cctctccatg gagggccggg
     4681 gggccaccgg ctggcggtac ctgcaccgta tggccctgaa agggatgaaa gtgccggacg
     4741 cgctggcgga gctggacggc agaacggtgg catttgaggt agagcgcacc gtcaagagcc
     4801 gcaggcggta tcaggaagtg gtgtccgggt atctgttcaa ccgccgggcg aacggcatcg
     4861 atgaaatctg gtacatctgc cctgaccggc cgacgcttgt ccgggtacag cgggcgattc
     4921 tggcggtgga tgaaatcgtg aatccacaga ccggcgaggc ccgcaaaacg gtggagctgg
     4981 accgggagag gctgttcgcg tgctttaaat ttatgacaac ggagtgaggg attatggcgc
     5041 tgtacatggt atttattgag gacgtggaag acaaagacgg cgagcgctgc attatgccaa
     5101 ccagcgagca tctggaagac atggtggtgc tcgcctggca gaccggggcg tatgttttct
     5161 gttttgtcga ccgggcccag catctggcga tgatgaaatc catcgggatg tatgacgagc
     5221 ggctcaacag cgacgaaaaa gccacgtact ttttcaccgg tgaaaatccg caatgggaac
     5281 gggacaaacc cacaggaatg aagctgtgtg attacctcta tcgaaaggtg gagcggttcg
     5341 ccatcgagaa cggtgtgcag gtggttgatg ccgggaagtg ctcaccggag cagattaacg
     5401 cgatgattga ggtggtcagc gagcggcacg gggaaagcgc ggtgaacgag aagctgaaag
     5461 cggagctgat gcgggaatat aacagccgct attttaacgt gccggtgcct gaactgccgg
     5521 aggacaaacc cgcccgccgg tggtggccgt tcggcaaaaa ataacgccag caccggcatg
     5581 gccggtgctg ttattcttcc ccgagcttat cccgcatcac ctgccagagt gtttccgcat
     5641 cctcgtgaag ccgttcgcac atgtcggccg gggtatccat gtccagctcg ttcagctttt
     5701 ccagcaacag cagcgactga tccttaatga atttagccat attgagggca gttttttcct
     5761 gcttcgtcat gttctccatt cccttcggta agttctcagc taaaccagcc ggaccagaag
     5821 cgttttttct tctgtgtcag ttctgttgtc aatctgtcca cttcggcctg gagtttccgg
     5881 cgctcatcat cgtgctggcg gcgttcttct tcccgggatg ttttatcttc cagcatcagg
     5941 gtgacagcct gccggagatc ggccagttcc ttttgcattg ccagaagctg ttcctgtggg
     6001 aaattttttt gtgtattttc acggtgtgaa atgccgggaa tttgtttctc agggatattc
     6061 acatttccgt atacacggat cagttctgac acatccacga cagggttgtt tttcccgtca
     6121 cgtgacacgg taacctttcc ctgttttaca tgattgtata gcgttcttct ggtaatgccc
     6181 gcggcttgtg ctgcctgtga aaggttgagc aatgttttcg ccactgtata cccctgattt
     6241 tatcgggtgt gaaaggtgtg taatttctca cggtaaatgt atcgctgaat tttacccgtt
     6301 tttccgttca ggatgtggtt ttctgacgcg ctgcgcttgt caaaccggcg acggccacgc
     6361 aatgaattgc gcccccgtca tcggttatta gaggctgtct gcccccggat acccggattc
     6421 tttctgatgc ctgacggtga cgatcacaat ctggtccatt tcccggtcgt ggcggtagag
     6481 catcacatag ccactgtcgc caaacccgat caccagttcc ttatattcca gcggcagaaa
     6541 cggcaccggg cggccaatgt ctggcaatgt tttcagttgc tggatagccc ggacgatcac
     6601 ctcacctcct tttctggctg ccagccgatt tttggttttg agaaagtcct gaaggcgttg
     6661 taaatcgcgc tgtgccagcg cggaaatcgt tacctgtggc atggcggagc atcctgctcg
     6721 ttctcagttc cccaggtact tatccaggct tccgcttctt ccgcagtaag gtgcagaccc
     6781 gtttcctgat agtgctgcca tgcggcctgt ccgtcccgca ggtactggct gcgcttctct
     6841 tcccggtcga tgtattcggt gattgcctcc agcattagcg cgtgcgctga gcggtgccgg
     6901 tcatcggcca gcgttttcag ccggtcctta agttcttcat cgagcctgat ggatgttgct
     6961 gatgacataa aagccccttt gtagtcatat gtattacaca tgactacttt aatccgtggc
     7021 ggcgctattt gccagaaata cggcttttgc tgcccatttt tcagcttcgc ttccactaat
     7081 cagggctgct acaggctcac tcatcatcca ggactattgc agcctgcggg ttaaaggacc
     7141 tgttactggc ggcaaccatc atcattgagc ttttgggtgg tgactggtcg cggatcctgt
     7201 aagccgtcat ttttcagact gaattcaaaa tttataacct gtcgcattgc cgggtgccct
     7261 gcgggatata gccagtgcct cattgccgtc agcgtttccc gaaaaaaaag gtgacagccg
     7321 gtaagcgaca cgttgtccac tcgtccatcg tccagaatat caaacgtggc ggtcacctga
     7381 ccttcgatgc gttttgctgc ggcctgtacc gggtaggttg gctgcagtgt ggttacgggt
     7441 tttagtggta tcgcatgtac cggagtgagg aacatggccg ccagcactgt cagagtaaaa
     7501 gctttcatgg tctgaaatcc agaggggcgt ttttgaatat ttgccaggta ttttcatccg
     7561 gtgccggcgg aattttggcg tgggtaacgg ctgatattgc tgctttacag agtttcggat
     7621 ctcctccttc agcaatggcg ctgttcagag tgccatcacg ttgtatggat atccgcacgg
     7681 tgcacatttt tcccgcatag gcctctggtt caaacatctg catttcaaca gcggtcttga
     7741 tggccgcagc gtagctactg attttagatg acttatccag agtataaatt ttcggcggct
     7801 gggtggtgga atgcttacag gccgtcagga gtaaggggct gcacaatgcc agcaggataa
     7861 agggtattaa acgcatcggg ttgatgacct gtcagtaaaa aatgttacat gtatatttgt
     7921 cactctttac ggccacgttc atttacataa acacggctta tcgtatcgtt tttacaccaa
     7981 acggcccgcc atgcagttgc gtgcataggc atgcagcggg ttacaattta ccgggcgcgt
     8041 ttccggcggt tttccgggtg gtttgttgcc tgttttatcc cgtagccgcc ggaaacgccc
     8101 tcagaccgtc tgagcggtgc gggcaatgcg acgttatggt aaatacccct taccggcggc
     8161 gctgtggctg ccaggtgaag gtggtttccg tagcctcgta tttctcccga cgcatttcct
     8221 gcgcccgcct ggcttcacgt tcgtcctgaa tggcacgaag caccctttcc cgcacctgtt
     8281 gttttaccgc ctttggcagc gttttgttcc ctttcaggcc catggccagt gcctgagcga
     8341 caaaggcgtc cgccaggcgc ttttcttcag cgggagtcag tttcaggtgc gtcatagtgg
     8401 cctccgggcg tctcaggttt cccgaacagt ttaaccgcag gtcctctcct catgccagtt
     8461 agctttcaga tgtgtctata ccatctgaaa tgtaagtgtg atgaatgtct taaataataa
     8521 aatttgttgc catgagaaat tttcgggaaa agacgcgatc tgcttacagg tggctccggg
     8581 cgcttttttg tgcctgaaga tgagaactaa tctcatatat tgacacctga aaacgggagg
     8641 agtaaggtaa taattatact gtgtatatgt acagtatgtt ggctttaatt tattgtttta
     8701 aatgtcaaag aggaaaacgg tatgagtggt ggagacggtc gaggtccggg taattcaggt
     8761 ctgggacata atggtggtca ggccagtggg aatgtgaacg gtacgtctgg taaaggtggc
     8821 ccttcatcag gtggtggtac ggatccaaac agcgggccgg gctggggtac gacgcatacg
     8881 ccgaatggag atattcataa ctataatccg ggggagtttg gtaacggcgg gagtaaaccc
     8941 ggtggtaatg gtggtaacag cggcaatcat agcggtagct ctggtggcgg acagtcttcg
     9001 gccactgcga tggccttcgg tctgcctgcc ctggctactc cgggggctga aggactggct
     9061 ttatcagttt ccggcgatgc gttgtcggcc gccgttgctg atgtgctggc tgccctgaag
     9121 gggccgttta agtttggtct gtgggggatt gcgatctacg gtgtgctgcc ttctgagatt
     9181 gcaaaagatg atccgaaaat gatgtcaaaa attatgacgt cattaccggc cgatgcggtg
     9241 acggagactc cggcaagtac tttaccactg gaccaggcga cggttcgtgt cagacaacgg
     9301 gttgtggatg tggtgaagga tgagcggcag catattgcgg ttgtcgcagg tcggccaatg
     9361 agtgtccctg tggtggatgc gaaaccgaca aaacgtccgg gggtattcag tgtgtcgatt
     9421 ccgggtctcc cgtctctgca ggtgagcgta ccgaaaggtg ttccgacagc gaaagccccg
     9481 ccaaaaggca ttgttgctga aaaaggtgat tcacgtccgg ctggttttac agccggtggt
     9541 aactcccgtg aggccgttat tcgtttcccg aaagagaccg gacagaagcc ggtttatgtg
     9601 tcggtgacag atgttcttac cccggcacag gtaaaacagc gtcaggagga agaaaagcgt
     9661 cgccagcagg catgggacgc cgctcatccg gaagaggggc tgaaaagaga ctatgataaa
     9721 gcgaaagctg agctggatgc cgaagataaa aatattgcga ccttaaacag ccgcattgca
     9781 tcgacagaga aggcgctccc cggtgcaagg gctgctgttc aggaagccga taaaaaggta
     9841 aaagaggcag aggcaaataa agatgatttt gtgacttata acccgcctca tgaatatggc
     9901 tccgggtggc aggatcaggt tcgctatctt gataaggata ttcagaatca gaatgagaaa
     9961 ttaaaagcgg ctcaggcatc tttaaacgca atgaatgaat ccttatccag agataaggct
    10021 gcacttaccg gggcgatgga gagccggaaa caaaaggag
//
