LOCUS       NZ_CP058206             2345 bp    DNA     circular CON 08-APR-2024
DEFINITION  Enterobacter roggenkampii strain RHBSTW-00002 plasmid
            pRHBSTW-00002_11, complete sequence.
ACCESSION   NZ_CP058206
VERSION     NZ_CP058206.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148444
            Assembly: GCF_013635755.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00002
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95.537547253764x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013635755.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 10:12:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,771
            CDSs (total)                      :: 4,659
            Genes (coding)                    :: 4,452
            CDSs (with protein)               :: 4,452
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 63 of 207
            Pseudo Genes (incomplete)         :: 146 of 207
            Pseudo Genes (internal stop)      :: 28 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            Pseudo Genes (short protein)      :: 1 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058206.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00002"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:1812935"
                     /plasmid="pRHBSTW-00002_11"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(143..355)
                     /locus_tag="HV027_RS23605"
                     /old_locus_tag="HV027_23615"
     CDS             complement(143..355)
                     /locus_tag="HV027_RS23605"
                     /old_locus_tag="HV027_23615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039509481.1"
                     /translation="MGDFERTYGAGADFDRIIDENNSEYFRDERQKAKNSKKEFSSFQD
                     ALVWAKANPGKTIVRSPDGVGFIEK"
                     /name="hypothetical protein"
     gene            616..804
                     /locus_tag="HV027_RS23610"
                     /old_locus_tag="HV027_23620"
     CDS             616..804
                     /locus_tag="HV027_RS23610"
                     /old_locus_tag="HV027_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(852..1079)
                     /locus_tag="HV027_RS23615"
                     /old_locus_tag="HV027_23625"
     CDS             complement(852..1079)
                     /locus_tag="HV027_RS23615"
                     /old_locus_tag="HV027_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_206659768.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSCMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            1933..2067
                     /locus_tag="HV027_RS24100"
                     /old_locus_tag="HV027_23630"
     CDS             1933..2067
                     /locus_tag="HV027_RS24100"
                     /old_locus_tag="HV027_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072035952.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDKAPLAAATGNRFRGGES"
                     /name="replication initiation protein"
     Typing          1553..2042
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.13"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 gcaagaattc ctgtaactaa caaaataggc cctgcaatgt acgtaacata gactccaaca
       61 tacgacactg tgaaacatat gagtacgtat atagccagtg ttgtccaaaa aatgactgcc
      121 gttttacagt gaatccactc tttcattttt caataaatcc cacaccgtct ggagatctta
      181 ctatcgtttt accaggatta gctttcgccc aaaccaaagc atcttgaaaa gaagagaatt
      241 cttttttcga atttttagct ttttggcgct catctcgaaa atattcgctg ttattctcat
      301 caattatccg atcaaaatca gcaccagctc cgtaggtacg ctcaaaatcc cccatttcct
      361 acccccataa atttaataaa aaaacagatt taaaatatcc aacagacact cttatttacc
      421 ataacatccg ttgcgtgcac cgcctgagag gctctcaggg cgtttctacg acctcacctg
      481 caaaagaaca catcttaccc cctgaaaacc gctcagaaac gcgctgatga tttctgcgta
      541 cctgcatggc tatgcactca agtgaatgaa gtaagatttc agtctgcgat caccataaaa
      601 ccgggaaaaa gccgtatgac aaaacaggaa tcagccgcac tgaacatggc aaaatttatc
      661 cgggcgcagt cgctgctgct gctcgaaaaa ctcgacgtgc tcgatcttga tgacgaggca
      721 gccgactgtg agcgaatgca cgaacaggca gaagcgcttt atcagaagct gagcgcacgc
      781 ttcggtatac aggacggcga ataaagagac gaaaagaacg ctcacttaga cgaaaagcgt
      841 atccagggca actatgtata tccaccgtgt acatagtcat gcctgacgct ttcgagcagg
      901 tcgagcatgc aggaacgacg agcttgattg gtatcggaca caatatggcg atacgcctca
      961 aacataacag cacccgcatc accgcccgaa cggcgaagca tagcggaaat catccgcaca
     1021 aagagcagac gttcatcggg gcaaaggttc ataagttctc ctggtgaaaa accaaacatg
     1081 aaagcaccca tctattaacg tcagctgtga acgcttcatt agaagaaaat aggtaaaccc
     1141 gggaaaaaga tccaaacgtt aacatcacag gtaaacagaa acgtcgggtc gatcgggaaa
     1201 ttctttcccg gacggcgcgg ggttgggcaa gccgcaggcg cgtcagtgct tttagcgggt
     1261 gtcggggcgc agccctgaac cagtcacgta gcgctagcgg agtgtatact ggctaaactt
     1321 cagagatgcc aaggaaggga caggatatgt gttgtagcac cgccaccacg gtggcgatac
     1381 tccaaaacgc ctggtcaggg cacagccccg acaccccaga gccgactgta ggtcactgga
     1441 cgaacgcggc ggaatgatgt cggatgacgt cattttgcgg cgtttgccct atcctgcatc
     1501 gtattaaaaa atgcagctat ggcgcgtacg gtgccaaaga taagcccgga gatattccgc
     1561 ttcctcgctc actgactcgc tccgctcggt cgttcggctg cggcgagcgg taccggctta
     1621 ctggcggggc ggaaatttcc tggcatcttc caggaagaag cttaacaggg aagcaagaag
     1681 gccacggcga agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     1741 cgctcaaatc agtggtggcg aaacccgaca ggacttaaag ataccaggcg tttccccctg
     1801 gtggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     1861 tatggccgcg tttatctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     1921 aagctggact gtatgcacga accccccgtt cagtccgact gctgcgcctt atccggtaac
     1981 tatcgtcttg agtccaaccc ggaaagacac gacaaagcgc cactggcagc agccactggt
     2041 aacaggttta gaggaggaga gtcttgaaat ttcgggtgcg ataaggctac actgaaagga
     2101 ctattttgtg actgtgctcc tccaagccag ttaccgcggt tcaaaagagc gtcaactctt
     2161 gcgaaccttc gaaaaagcca ccttgccggg tggttttttc gtttacagag ctaagagatt
     2221 acgattctcc ctaaatgatc tcaagaagat ccgtttaaaa aagcgggttt tatggttgga
     2281 tgaagttcgt cttacccagt cacatattag atttatttga attactacta ggtttcgtaa
     2341 gccat
//
