LOCUS       NZ_CP058205             2495 bp    DNA     circular CON 08-APR-2024
DEFINITION  Enterobacter roggenkampii strain RHBSTW-00002 plasmid
            pRHBSTW-00002_10, complete sequence.
ACCESSION   NZ_CP058205
VERSION     NZ_CP058205.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148444
            Assembly: GCF_013635755.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00002
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95.537547253764x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013635755.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 10:12:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,771
            CDSs (total)                      :: 4,659
            Genes (coding)                    :: 4,452
            CDSs (with protein)               :: 4,452
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 63 of 207
            Pseudo Genes (incomplete)         :: 146 of 207
            Pseudo Genes (internal stop)      :: 28 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            Pseudo Genes (short protein)      :: 1 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058205.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00002"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:1812935"
                     /plasmid="pRHBSTW-00002_10"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(958..1143)
                     /locus_tag="HV027_RS23595"
                     /old_locus_tag="HV027_23605"
     CDS             complement(958..1143)
                     /locus_tag="HV027_RS23595"
                     /old_locus_tag="HV027_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1509..1736)
                     /locus_tag="HV027_RS23600"
                     /old_locus_tag="HV027_23610"
     CDS             complement(1509..1736)
                     /locus_tag="HV027_RS23600"
                     /old_locus_tag="HV027_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          824..911
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(4..197)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.265"
                     /coverage="98.0"
                     /evalue="1.5100000000000005e-58"
     Typing          complement(1972..2478)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.774"
                     /coverage="99.0"
                     /evalue="6.33e-132"
ORIGIN
        1 tctgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttggcg cggccttatg
       61 ctttcttcgt taagtatctt cctggcatcc cccaggaaat ttctgatcca tccgtaagcc
      121 cgtcccgctc gccgcagccg aacgaccgag cggagcgagt cagtgagcga ggaagcggaa
      181 tatattctgt atcacatttt ctcctgacgc gttttctttc actttctgcg cctgtcttat
      241 gtggcattaa tgctatgtgt tactgccatg ctacatctta agccagtata cactccgcta
      301 gtgctccgtg actggtccgg cgctgcgccc ggaacccgcc tgtaccggtt cagcagccgt
      361 tccggcctga ctgcaatttt ttttttttca tccctgcccg ctaccctgta aacctttctt
      421 ctgcgttgcc gttaacagta ggtaagccat gtttggtttt tcttccggag ccctgatgac
      481 tcttagtgat gatgagcgac acctgcttgt gtccgttgtt tcggtctggc tacggcgtgc
      541 cggaggggat gtcggagcca tgatgctgga tgcataccgg cagattttgt ctgaaaccga
      601 accggccgta aggactgtca tgctcgaatt tcttgaatcc gttcgcattc attacatttc
      661 gtcctgagca ttttctgtat ttctgtccat acttaaacga ggcggccgtc acggacggtg
      721 gtcgtcgttc catgatgctg ttatggacga gtgagagtaa tctaatctaa cttgattgag
      781 ttccgggcgt gccgcccggt tttttttgct gtttcccggt gtccgttccg ggacaggatg
      841 tgcatttgta ataccgcgca cgcgccggca tcacaaatac cctggtcagg gctgcgcccc
      901 gacaccccgc catcgcctca cggccgcgca ctgaattgcg cacccgttca ccgattttca
      961 cgcattttcc tcgcctatct gtgcgttcag catggcgtaa agcgcttcgg cctgatcgtg
     1021 caggtgctca cagcagccgg cagcctcatc cagatccatc tgctcgagcc gctcaaggag
     1081 gagaagtgtc tgtgcgcgga taaatttggc catattgagg gcggcggatt cctgtctggt
     1141 cactggtgat tttccccgtc tggtgatgat ttttgcgtga aaagagcatg gcgtcattct
     1201 ggtgcatagt catgcatgcc gttaaaattt aacaggcgcg tttctgacgg gttccggggt
     1261 ggtttgttgt ggtttttgtc atggtttcgt caggaacgcg ctgaacgcgt ctcaggcggt
     1321 gcgggcaacg gatgttatgg taaattcttg ggggcttgat tataaaatac cgcacttcct
     1381 cttatgtctt gtgcttttta gacaaccctg aaagctttta gctttggtga tgcaatttgc
     1441 tgggattttg gcggagaacc acaggtaaag aaaaaggcca cattagcggc ctttttcgga
     1501 gaagatgctc agtgcggaat ttcggagact tctttaacgt cggttttatt gatctgctgt
     1561 ttgacaccat tcgcgtctgt atagccaagc aggcctgagt ctgattcttt aggtttgccg
     1621 tcgctgacaa tagtgcgacc gtcattggtg tgtatggcat agcttgtgcg cgtgcagccg
     1681 gtgagggctg aaagcgcaac tgccgctgcg aacactgaaa caaaaatctt tttcaaagcc
     1741 agctcctttt atccatatca gttttggtca ctaaccatct aactataggt cattttttat
     1801 gtgaaaagag gcttgatggt gggctgtata tcgagttagg cgcgaggtgc tatggtcaaa
     1861 gtgtggtgtc aggcggggag gaggcttata tgagatgtaa tccggccgat acatatacca
     1921 agatgattta agtattcctc tttgtgaggt tggatgtcta atttataaag gatcttcttg
     1981 agatcctttt tttccgcgcg taatctcttg ccctgtaaac gaaaaaacca ccctggcagg
     2041 tggtttttcg aaggttaggt aatcctggca gatcccctaa ccgtggtaac agtcttgtgc
     2101 gagacatgtc accaaatttg tcctttcagt gtagcctcac taaggccgcc acttcaagaa
     2161 ctcttgagac atctctcgca catcctgttt gccaatggcc gttgccaatg gcgattagtc
     2221 gtgtctttcc gggttggact caagttgata gttaccggat aaggcgcagc ggtcggactg
     2281 aacggggggt tcgtgcatac agtccatcct ggagcgaact gccttcccgg aactgagtgt
     2341 caggcgtgga atgagaaacc gcggccataa cagcggagtg acaccggtaa accgaaaggc
     2401 aggaatgcgg gggagcacga gggagccacc agggggaaac gcctggtatc tttaagccgc
     2461 atcgggtttc gccaccactg atttgagcgt cagat
//
