LOCUS       NZ_CP058204             3141 bp    DNA     circular CON 08-APR-2024
DEFINITION  Enterobacter roggenkampii strain RHBSTW-00002 plasmid
            pRHBSTW-00002_9, complete sequence.
ACCESSION   NZ_CP058204
VERSION     NZ_CP058204.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148444
            Assembly: GCF_013635755.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00002
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95.537547253764x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013635755.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 10:12:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,771
            CDSs (total)                      :: 4,659
            Genes (coding)                    :: 4,452
            CDSs (with protein)               :: 4,452
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 63 of 207
            Pseudo Genes (incomplete)         :: 146 of 207
            Pseudo Genes (internal stop)      :: 28 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            Pseudo Genes (short protein)      :: 1 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058204.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00002"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:1812935"
                     /plasmid="pRHBSTW-00002_9"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            691..1659
                     /gene="mobV"
                     /locus_tag="HV027_RS23580"
                     /old_locus_tag="HV027_23590"
     CDS             691..1659
                     /gene="mobV"
                     /locus_tag="HV027_RS23580"
                     /old_locus_tag="HV027_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_181239579.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSESTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPQQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDSQQKRLQSVTEPFKGLSREQM
                     THILTMAQTFQQQNHDREKQRQLEREQERQQRQKTDRGISR"
                     /name="mobV"
     gene            1656..1847
                     /locus_tag="HV027_RS23585"
                     /old_locus_tag="HV027_23595"
     CDS             1656..1847
                     /locus_tag="HV027_RS23585"
                     /old_locus_tag="HV027_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_023063538.1"
                     /translation="MSEKQRREGFRKAEASLRLEGLDPSGTPHYELVKARIISGEISYE
                     QGRKEIFDYYVQKARSKE"
                     /name="antitoxin VbhA family protein"
     gene            complement(2255..2806)
                     /locus_tag="HV027_RS23590"
                     /old_locus_tag="HV027_23600"
     CDS             complement(2255..2806)
                     /locus_tag="HV027_RS23590"
                     /old_locus_tag="HV027_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_181239578.1"
                     /translation="MSLVRYKTNPFLDDMIVPVKGQKVQISKLGKDNNILVNQSTGEVH
                     GTHVTTYRKVDSEQFVKLFTANIALTFQLSSAGIKAFGVLLWVVQNKALSKDEVDLDSY
                     ALDEFYELHKDSEPPLRLSHPTFKRGINELEKAQIVAKTLRKGRYFINPNFVFNGDRVA
                     FTTVIERKKAESPKIKEISS"
                     /name="replication/maintenance protein RepL"
     Typing          683..1554
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.502"
                     /coverage="86.0"
                     /evalue="0.0"
ORIGIN
        1 ctggatcgtc aatgattaaa tcgcggggtc ccgccgcaag cggtgggttc cgatttaacc
       61 attgatgact caccgtaaaa caaatgctta tcaggggtgg atggtttcac gcagtgaggc
      121 catccacctg ccagacgctt tttctgccaa accccaagaa gattttttat cggacaccag
      181 gtaaaaaacg actttgccct tgacctcgat gttgtcgtga tccctgacaa cccacacgaa
      241 tacaaaaagt tcgccatacc tcaaagtcgt gatgcgaccc tgttcgggcg tctgcaggaa
      301 ggtttgtttt gtatggcctg cgatacaacg gctaccgttt cgggccaaag cacagaccga
      361 ttatcttctt gtcatagccg gatgttgtga agcgacggcc ggtacaaaaa ccaatacgga
      421 gaaagactaa atgagataca cacatgtttt ggaaattgtc tggcaattgt tcaatttcag
      481 catcgagctg ctgagattta tcaacgacac tttaccgctg ttgttccctg gcggttgatg
      541 ttgtttttcc tgtctggcgt gagccagacc gaacgatttt gcgaagcaaa gtctagtgag
      601 tacactatca atctgatagt ggcccgccgg aggcatcgca atgcgatccc acgtctggcc
      661 ggatggacgc cggagcgagg ggggggaagc atggcgaact acgcgataat gcgctgtaaa
      721 aagctgacgg gaatgggcag tgtggccagc gctttgcagc actgctacag ggagcgtgag
      781 acgccgaatg ctaacgctga acggacacca gaaaactact gctctgtatc cgaatcaacg
      841 gatcaggcaa tggggcgtgt ccgggagctg ctgcctgaaa aacgccgtaa agatgctgta
      901 ctggccgttg aatacgttat gactgcaagc cctgagtggt ggaaagaagc gacgccgcag
      961 caacaggcgg agtttttcgc ccgctctgag caatggctgg agaagaaata cgggaaggat
     1021 cgtgttgtgg cggcggtggt tcaccgggac gaggccaccc cgcacttgtc agcgttcgtg
     1081 gtgccattga cgcaggatgg acggctgtcc gcgaaagagt ttattggcgg gcggtcaaag
     1141 atgcgtgaag atcagagcac ctacgctgaa tcagtaaaaa aactgggtct tgagcgcggc
     1201 atcgagggaa gccgtgccac gcatcagaca gttcagcact actacgaatc gataaaccgt
     1261 ggcacccgta gccaggtatc gatctcgccg gaggcgctgg agccgcgtgt attgcggaag
     1321 ggcattttca ccaaagacgt ggaagatcag gcagccatag ccaaacgtct aagccacgca
     1381 gtgaacgatg gctttgcagg aaccatcgct atggcctctc agagcgctca gaatgcaaaa
     1441 agagcgcggg agctacagaa gactatggat tcgcagcaaa aacgcctaca gagcgttaca
     1501 gagcctttta agggactttc ccgggagcag atgacccata ttctgacgat ggcgcagacg
     1561 ttccagcaac agaatcacga tcgtgaaaaa cagcgccagc ttgaacggga gcaggaacgc
     1621 cagcaacgtc agaaaactga cagaggtatt agccgatgag cgaaaaacag cgacgtgaag
     1681 gttttcgcaa agcggaagcc tcattaaggc tcgaagggct ggatcccagc ggaacgcctc
     1741 actatgagtt agttaaagcg cgcattattt cgggtgaaat cagctatgaa cagggacgaa
     1801 aggagatttt tgattattac gttcagaaag ctcgctccaa agaataagaa ctcatattaa
     1861 gacacaatat ttatatttat ttaaaagcgt gaaaatatat tttccatttt atataaaata
     1921 tatttgcttt ttatgtttat tataatatat aaagtttctg ttacttttta tttatatttt
     1981 taagtaagaa gaacggcgag taatgtcgtc aatataaata ataaaatatg aggctacgca
     2041 tgaataaaaa agaaaaagta gccaagcgta tgagaataat aaaaataacg attgttatta
     2101 tttctatgta cccttggtct gtttgttggt gaaagtaaaa agcagctttt agctgctttt
     2161 ttattttgga tgcatccgtt ttccattagt tacacagggt aggcatgctg cggatgcttg
     2221 ataggtagtc cttggttgag ttctgtgagt cttatcaaga cgaaatctct tttatttttg
     2281 gggactctgc tttttttctt tcaatgacag ttgtgaaagc aactctatcg ccattaaata
     2341 caaagtttgg gttaataaaa tagcgacctt tccgaagtgt ttttgcaaca atctgggctt
     2401 tttctaattc gtttatcccc cgtttaaaag tagggtggct tagtcgcagt gggggttctg
     2461 agtccttatg taattcataa aactcatcta aggcatatga atctaaatcg acctcatctt
     2521 ttgagagagc tttgttttga acaacccata ataaaacccc gaatgctttt atacctgctg
     2581 atgacaactg gaaagttaaa gcaatattgg ctgtgaaaag ctttacgaac tgctctgaat
     2641 caacctttcg atatgtagtt acgtgcgttc cgtgtacctc acctgtcgat tggttaacca
     2701 agatattgtt gtcttttccc agcttagaga tctgcacttt ctgtcccttt actggaacaa
     2761 tcatatcatc caaaaatggg ttagttttgt atcttactaa gctcattatc tacccctaaa
     2821 ttgataccat caattcgatt ttagatagcc gcaagctcaa aatcaacaac tattttgagc
     2881 ttacaaagta cgtagattga gccttgaagg tactgatttt gaaaatacga tccttgctac
     2941 atgcgggttt gcgagtagtg ccttcttagt tcttatctat cgctaagaac taagaagaaa
     3001 aaacagaggt cctgagcgaa agtgcctgag gtagtttccg tgatattccg cttcctcaat
     3061 cggacagact cgcggcgttg ccgctcccta atccgatctt gcggcgagcg gtccttgctc
     3121 tctcaaaacc aattccatta g
//
