LOCUS       NZ_CP058203             3573 bp    DNA     circular CON 08-APR-2024
DEFINITION  Enterobacter roggenkampii strain RHBSTW-00002 plasmid
            pRHBSTW-00002_8, complete sequence.
ACCESSION   NZ_CP058203
VERSION     NZ_CP058203.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148444
            Assembly: GCF_013635755.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00002
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95.537547253764x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013635755.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 10:12:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,771
            CDSs (total)                      :: 4,659
            Genes (coding)                    :: 4,452
            CDSs (with protein)               :: 4,452
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 63 of 207
            Pseudo Genes (incomplete)         :: 146 of 207
            Pseudo Genes (internal stop)      :: 28 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            Pseudo Genes (short protein)      :: 1 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058203.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00002"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:1812935"
                     /plasmid="pRHBSTW-00002_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            130..1074
                     /locus_tag="HV027_RS23555"
                     /old_locus_tag="HV027_23565"
     CDS             130..1074
                     /locus_tag="HV027_RS23555"
                     /old_locus_tag="HV027_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_181239574.1"
                     /translation="MGLLIRPQATALGYSHIQFNPPPHVAMLVFDVDKKHQHDPQRDGY
                     HEWQDRGLPAPHWISINPENGNYHLGYLLASPVARSSVARLGPLRYLAAIEHVLAKRLG
                     ADLRYAGLVTKNPVHSDWMTFWHDIEPYTLDYLAEFCPDADLAAFSGRKRKEASGLGRN
                     IEVFDNVREWAYKAVRRFWRPNGYDAWADAVLAACESANAFGLEQGGPLPVSEIKSTAK
                     SIARWVWRNLTPSSFADYVDRTHTPEIQARRGAKGGKVSKGGGRPSNSGKDKSDLLPEV
                     LRLKAQGYTNRDIADDLQISPSTVSVYLKRDHP"
                     /name="replication initiation protein"
     gene            1475..1693
                     /locus_tag="HV027_RS23560"
                     /old_locus_tag="HV027_23570"
     CDS             1475..1693
                     /locus_tag="HV027_RS23560"
                     /old_locus_tag="HV027_23570"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181239575.1"
                     /translation="MPTEYDYQITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFR
                     DEKALQTLLDSLYEDALAEASAWLASR"
                     /name="hypothetical protein"
     gene            1675..2013
                     /locus_tag="HV027_RS23565"
                     /old_locus_tag="HV027_23575"
     CDS             1675..2013
                     /locus_tag="HV027_RS23565"
                     /old_locus_tag="HV027_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_181239576.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLREICPKAQLAEWMREQCLGVV
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1994..3448
                     /locus_tag="HV027_RS23570"
                     /old_locus_tag="HV027_23580"
     CDS             1994..3448
                     /locus_tag="HV027_RS23570"
                     /old_locus_tag="HV027_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011116726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_249415534.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASERRVHEARESYLRGVEIKRGENERRY
                     CRAQPEHGLAVAQNVDLDAGQHRSVLERNERDPLVSGGIDSGAASQSPSLGGEGRQDKA
                     GPVREEVGAPLRADLRFQPGRLGEGYVLGRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQARGREQPGSGETLDVASGRLNAASGRLEQATGGLDRASQQVA
                     KEREHRLLKVLERGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          1995..3445
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.975"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agcataacac atgcacgatc tcagacaacc ctgcaccgcc aataagcgcg gtgatctctg
       61 actcagacgg cccaaaacct cttcatagac aggcttccgc gccgccccta ctgcaccaac
      121 gacaaatcga tggggctgct cattcgtccg caggctactg cgctgggcta tagccacatc
      181 cagttcaacc cgccgccgca tgtcgccatg ctggtgttcg acgtggacaa gaagcaccag
      241 catgaccccc agcgcgatgg ctaccacgag tggcaggata gggggctgcc tgccccgcac
      301 tggatctcga tcaacccaga gaacggcaat taccaccttg gttacctgct ggcctcgccc
      361 gtggcgcgat ccagtgtcgc cagattgggg ccgttgcgtt acctggctgc catcgagcac
      421 gttctagcga aacgattggg ggccgatctg aggtatgcag ggctggtcac caaaaacccc
      481 gtccacagcg attggatgac gttctggcac gacattgaac cctacaccct cgactatctc
      541 gccgagtttt gcccagatgc tgatctggcg gcctttagtg gccgtaagcg caaagaagcg
      601 agcgggttgg ggcgtaacat agaggtgttc gataacgtgc gggaatgggc ctataaggcc
      661 gtcagacggt tttggcggcc caacggctat gacgcatggg ccgatgccgt gcttgcggcc
      721 tgtgagagcg ccaatgcgtt tggcttggag caaggcggtc cgctgccagt gtccgagatt
      781 aagtctacgg ccaagagcat tgcccgctgg gtctggcgga acctaacccc atcctcgttt
      841 gcggattatg ttgatcggac acataccccc gagatccaag cgcggagagg ggctaagggc
      901 ggtaaggtat ccaagggagg gggaaggccg agcaatagcg gtaaggacaa gtccgatcta
      961 ttgccggagg ttctgcggct taaggctcag gggtacacca accgcgatat agcggacgat
     1021 cttcaaatct cccccagtac ggtgagcgtt tacctaaagc gtgatcatcc gtgatcgaaa
     1081 caagcctata tcagataaca gcgcccgaaa gggcgttctc gtgcttttgc cttttagtct
     1141 taaaaatcct tttagcggag agatctggcg aggtgggtaa ctgcgcccgt ccgtccatcg
     1201 ccccccctgt ttcttcgggg ggtgtgggcg gcagcagtta tccacggagc agctcttgcc
     1261 cttgatctag atctagggct tgcagtatcc cgcccatggt tgaataatgc attaagcaag
     1321 gctacacaga ggcccctcta cctgcttcgc ggtctcgggc ctctgtggcc ttgttcaggg
     1381 gtgctacgcc cccccgaaac cccctgtggc ccccgctcga tccgcccctt cgggacggtc
     1441 tgcgcggcgg cttcttagct ctggagaatg gacgatgccc actgaatacg actaccaaat
     1501 taccccccgc actctcgggc ttggcggggg ctggaatctg cgtttgctgg agaacggcga
     1561 agaggtcggc gggggtgttt tccccttgcc ggagcactgc gattttcggg atgaaaaggc
     1621 gctccagacg ctactcgata gcctctatga ggacgcctta gcggaggcct ctgcatggct
     1681 ggccagccgt taaagcgcct ccgctctatc aagatccggg tctcagatgc ggagcttgag
     1741 cgcctgcggg agatctgccc caaggcccaa ctggcggagt ggatgcggga gcaatgtctc
     1801 ggcgttgtgc agccccagcg ccgaacgccg gcgcctaccg tagatccggc gctcttgcgg
     1861 cagttggccg gaatgggcaa caacctgaac cagatcgccc ggcgcgtaaa ttccggggag
     1921 tgggggccgt tggatcggct gcgcatcata gcggagctgt ccgctatagg ccgcgagctg
     1981 gaggaactgc accatgatca tcaaattcca taaccgtggg gtaggccggg gctccgggcc
     2041 tgttggctac ctgttgggcc gtgacggcaa ccgggaaggc gcaacgctcg atcggggcaa
     2101 ccctgcggtg attgaagccc tgattgattc cagtccctac gccaaaaaat acacctctgg
     2161 cgtcctgtcg tttgccgagg ctgatttacc aagggcgacc aaggacaagc tgatgagcga
     2221 ctttgaaaag gcgctcctgc ctggactgga ggggaaccag tacgctgttt tgtgggtaga
     2281 gcaccgagac aaggggcggc tggagctgaa ttttgtagtc ccgaacatcg aattgcagag
     2341 cgggaagcgg ctgcaaccct acttccacaa agccgacaat ccgcgcctgg atgcctggcg
     2401 aaccgtggta aacggggagc tgaaattgca cgacccggac gatccgttaa accgtcaggc
     2461 cgttatcacg ccaaaggatt tgccgagggg gcacaaggag gccgctcagg ctattacaga
     2521 cgggcttttg cgcatggctg gggcagggga gatccagagc cgcgatgatg tgcgcaaagc
     2581 cttgcagggt gcgggtttca gcgtggtacg cgaaacaaaa aacagcctga gcattgccga
     2641 cccggacggg gggcggaaca tccgactaaa aggggtgctg tatgagcaag attttagaca
     2701 tggcgaagga cttcgagcag aactcgaagc agcaggccaa cgctatcgag aagcgtctga
     2761 gcgaagagtt cacgaggcac gagaaagcta tctccggggc gttgaaatca agcgaggaga
     2821 gaatgagcgc cgctattgcc gagcacaacc agagcatggg ctggctgttg ctcaaaacgt
     2881 ggatctggat gctggtcagc atcgttctgt tcttgagcgc aacgagcggg atcctttggt
     2941 atcaggggga attgatagcg gagcggcaag ccaatctcca agccttggcg gagaagggcg
     3001 gcaagataaa gctggcccgg tgcgggaaga agtcggagca cctttgcgtg cagatctccg
     3061 attccaaccc ggacgtttgg gggaaggata cgttttgggt cgtgccgaag gggtactaaa
     3121 tgacgggatt ggagcgggaa ttgctctgcg cgttcgaggc tttgcagagc gcatacgagc
     3181 agcagcacaa ggcatggcag gaaagctacg cgagcttggc gagcatgtac gagagcacca
     3241 agcgagaggc cgagagcagc cgggaagcgg cgaaacgctt gacgtggcaa gtggacggct
     3301 taacgcagca agtggacggc ttgagcaagc aactggaggg cttgaccggg ctagtcagca
     3361 agttgcaaaa gaaagggagc acaggctcct gaaggtactg gaaagaggct gggagcgtgg
     3421 agacagatcg cgaggctttg agcgatagcg cccagtagga tcaggacgtt ttttggtgta
     3481 acgtactgtc cagaaaaaac aaaccccgaa tctctgttaa tcaacttggc gggagattca
     3541 gagagttcgg ggccgttcac aaagaacagg aaa
//
