LOCUS       NZ_CP058202             3966 bp    DNA     circular CON 08-APR-2024
DEFINITION  Enterobacter roggenkampii strain RHBSTW-00002 plasmid
            pRHBSTW-00002_7, complete sequence.
ACCESSION   NZ_CP058202
VERSION     NZ_CP058202.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148444
            Assembly: GCF_013635755.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00002
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95.537547253764x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013635755.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 10:12:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,771
            CDSs (total)                      :: 4,659
            Genes (coding)                    :: 4,452
            CDSs (with protein)               :: 4,452
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 63 of 207
            Pseudo Genes (incomplete)         :: 146 of 207
            Pseudo Genes (internal stop)      :: 28 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            Pseudo Genes (short protein)      :: 1 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058202.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00002"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:1812935"
                     /plasmid="pRHBSTW-00002_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            124..987
                     /locus_tag="HV027_RS23525"
                     /old_locus_tag="HV027_23535"
     CDS             124..987
                     /locus_tag="HV027_RS23525"
                     /old_locus_tag="HV027_23535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071032989.1"
                     /translation="MIIQDNHTIEIFKNQLPVKPYCSNNLQSGLSIRNKNRALEMLYLQ
                     ANQPAIQTCLLFDLDEDNAFYKFDEVGLPVPTFITKSPDSGRCHYGYMLKAGVCKTQHA
                     KLKPLKYAAAVEAGIAGKLASDRGYAGLITKNPLHSHWSPFWSGADLYELDYLADFVEL
                     ETPKKAKKSENYGLGRNVNLFEDLRTYAYRRVLKFKKDSTYEAWERDVLSIAEGLNNHC
                     NALNPLQYNEIKATARSVSRWTWKHFDSATFSDIQSNRGKKNLGKTKNTNVKSEMLEIL
                     MSKGAL"
                     /name="replication initiation protein"
     gene            984..1244
                     /locus_tag="HV027_RS23530"
                     /old_locus_tag="HV027_23540"
     CDS             984..1244
                     /locus_tag="HV027_RS23530"
                     /old_locus_tag="HV027_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047739397.1"
                     /translation="MIINGKKIKAKEIMEMTGRSERTVRKYFSQSREVYEDEAAERRRK
                     AYELREKGLKWKDVGKELDTSLHGAVALYKRYLKLDLNQQQ"
                     /name="hypothetical protein"
     gene            complement(1261..2742)
                     /locus_tag="HV027_RS23535"
                     /old_locus_tag="HV027_23545"
     CDS             complement(1261..2742)
                     /locus_tag="HV027_RS23535"
                     /old_locus_tag="HV027_23545"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_181239573.1"
                     /translation="MLILVRAKARKKIKQQCPLTQTKVCSGPCGAKVKGKKIRFPFMAI
                     YSFQCKIVARTKRLNNAVAHAAYINRIDLDNNRDGSKWRYGHSHEDVYKSGVILPDNAD
                     EKFSDPAYLWNEVEKKENRSNSRLARSFIISLPNELTPEQNKNLIEDYIKTHMTNKGMI
                     VNYAIHIDDTNNFHAHLMSTTRELDKSGLEFAKKNVIGRTWNDDDFLEGLRKGWADTAN
                     THLARLDHQVRIDHRTLSAQRDEALEQFEMATTAEAKAEALAKAVSLDRPAIQRIWRSK
                     LNTEEGRALREAQQDRRDVQLVVAEETKKSVLSLPQEIVLNNFAPKKIKSKSKTKTTIE
                     KTRPAPEAVLKTADTGGEIKDLKYSSFSKLMLNIQIMYNEHKKKSIGKKLNKRIKKIEK
                     ADKIGGGKGGYFARHLDNDYSVEDNIKPIPEERKEDEYTKLQKFFDDVNKRGIELNNKK
                     EAMLKLEQENKKALSQQPKHKPKDNNENDRHIKYK"
                     /name="MobA/MobL family protein"
     gene            2847..3038
                     /locus_tag="HV027_RS23540"
                     /old_locus_tag="HV027_23550"
     CDS             2847..3038
                     /locus_tag="HV027_RS23540"
                     /old_locus_tag="HV027_23550"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_147717131.1"
                     /translation="MLTVVYCIWSVIGLGIIILALWAAISKSTEAKINKDDDIRSMKKR
                     LFKIEERLKLDSDDDYDY"
                     /name="hypothetical protein"
     gene            3046..3363
                     /locus_tag="HV027_RS23545"
                     /old_locus_tag="HV027_23555"
     CDS             3046..3363
                     /locus_tag="HV027_RS23545"
                     /old_locus_tag="HV027_23555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181239572.1"
                     /translation="MTKKMEKIEDRVSYHQKQLAQLKAKKKEELSKEKKAEKAKRASKN
                     ILLGASICKVLGIDHTEIDEYLPKIVGLFSSTVQYINDPKVLAKRTDLLESWKNGGDRN
                     E"
                     /name="hypothetical protein"
     gene            complement(3510..3806)
                     /locus_tag="HV027_RS23550"
                     /old_locus_tag="HV027_23560"
     CDS             complement(3510..3806)
                     /locus_tag="HV027_RS23550"
                     /old_locus_tag="HV027_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071032991.1"
                     /translation="MKDKRKLGFLRGQGSIPDQKQLEEMDQEVSSQFFRDLMESAQEAV
                     EIHQGKRPAARRTLFHVNPVTKSTIERSEARTDVFAATDAPDLFKKLKLDRDD"
                     /name="hypothetical protein"
     Typing          125..987
                     /name="rep_cluster_1451"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.884"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1265..2616)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttagatcg gttaaaaccg ttacagattt gaaaaatata acaaaataaa aaccccgctt
       61 cctgtggact gtttggcgac aaacacaaga gagcggggcc agataaaaaa ggactgctta
      121 aaaatgataa tacaagataa ccatactata gaaatattta aaaatcaatt acctgtaaag
      181 ccttactgct ccaacaactt gcaatcaggc ttgagtatca ggaacaaaaa tagagcactt
      241 gagatgcttt accttcaagc aaatcagcca gctatacaaa cctgtttgct gttcgatctc
      301 gatgaggaca acgctttcta taagttcgat gaagtagggc ttccggttcc tacgttcatc
      361 actaaaagcc ctgattctgg taggtgtcat tatggctata tgcttaaagc cggtgtctgt
      421 aagactcaac acgcaaagct gaagccattg aagtatgcag ctgctgttga ggctggtatt
      481 gctgggaagt tagcttctga tcgtgggtat gccgggctga ttaccaaaaa cccactgcat
      541 agccattggt cgccgttctg gtcgggtgct gatttatatg agctggatta tttagctgat
      601 tttgttgagc tggagacacc aaagaaggca aaaaaatcag agaactacgg gctagggcgt
      661 aatgttaacc tgtttgaaga tttaaggact tatgcttata ggcgagttct gaaatttaaa
      721 aaggactcaa cttatgaagc atgggaacgt gatgttttaa gtatagctga aggactcaat
      781 aatcattgta atgctttaaa tccgctacaa tataatgaga taaaagcaac tgcgagatct
      841 gttagtcgtt ggacttggaa gcattttgat agtgctactt tttcagatat acaatcaaac
      901 agaggtaaaa agaacttagg taaaacaaaa aataccaacg taaaatctga gatgttagaa
      961 atattaatga gcaagggggc attatgatca ttaatggtaa gaaaataaaa gcaaaagaga
     1021 ttatggaaat gacaggccgt agtgaaagga ctgttaggaa atacttttct caatctcgtg
     1081 aagtctatga agatgaagcg gctgaacgta gaagaaaagc gtatgagctg agagagaaag
     1141 gcttgaagtg gaaagatgta ggaaaagagt tagatacttc tctacatggt gctgtagctc
     1201 tttataagcg ttatttgaag ctagatttaa accaacaaca atagcaggtt tatggcctgc
     1261 ttatttgtat ttaatatgcc tatcattttc gttgttatct tttggtttat gcttaggttg
     1321 ttgtgatagt gcttttttat tttcttgttc gagtttaagc atggcttctt ttttattgtt
     1381 tagctcaata cctcttttat tcacatcatc aaaaaacttc tgtagcttcg tatattcatc
     1441 ttctttgcgt tcttctggta taggtttaat attatcttca actgaataat cattatctaa
     1501 atgtctagca aaatagccac ctttaccacc gcctatttta tccgccttct ctattttctt
     1561 tattcttttg tttagttttt taccaataga ttttttctta tgctcattat acattatttg
     1621 aatgtttaac attaacttac taaaagaact atatttgaga tcttttatct ctccccctgt
     1681 atcagcagtt tttaaaactg cttctggggc ggggcgtgtt ttttcaatag tagtttttgt
     1741 tttggatttt gattttattt ttttaggagc aaagttattt aaaactatct cttgtggtaa
     1801 agacaaaaca ctctttttag tctcttcagc aacaacgagc tgcacatctc ttctatcttg
     1861 ctgtgcttca cgtaaagccc taccctcttc cgtgttcagc ttactacgcc aaatcctttg
     1921 gattgctggc ctatcaaggc ttacagcctt agccagagct tcagctttag cctctgcagt
     1981 ggtagccatt tcaaactgtt caagggcttc atcacgttga gctgatagcg ttctatggtc
     2041 aatcctaact tgatgatcaa gccttgctaa atgagtattg gcggtatctg cccagccttt
     2101 gcgtagacct tcaaggaaat catcatcatt ccacgttctg ccgataacat tcttttttgc
     2161 gaactctaaa cctgatttat ccagttctct tgtggtgctc atcaaatgag catgaaagtt
     2221 attagtatca tctatatgaa tagcatagtt tacaatcatg cctttgttgg tcatatgggt
     2281 tttaatgtaa tcttcaataa gatttttatt ctgctctggt gttagttcgt ttggtaaaga
     2341 aataataaag ctgcgagcca agcgactatt agatctattt tctttcttct ctacttcgtt
     2401 ccataaataa gctgggtcag aaaatttttc atcggcatta tctggaagga taacaccaga
     2461 tttatataca tcttcatggc tatgaccata acgccatttt gagccatcac gattattatc
     2521 aagatcaata cggttaatat atgctgcatg agcaacagca ttatttaaac gttttgttct
     2581 tgctacaatc ttacattgaa aactatatat agccataaat ggaaacctta tttttttgcc
     2641 tttgacctta gccccgcagg gaccactaca aactttagtt tgtgtaagtg ggcattgctg
     2701 ttttattttt ttcctcgctt tcgctcggac taaaataagc aatgtcgctt tacttatatt
     2761 tattaaataa catacaaaaa tacttttgac aaatatttat aaatatatta ttgaataagt
     2821 aataaaataa ataatagggg gtttctatgt taactgtagt ttattgtatt tggtctgtta
     2881 tcgggcttgg cattattata ttggcattat gggctgcaat atcaaaatca acagaagcaa
     2941 agataaacaa agatgatgat attagatcca tgaaaaaaag actgttcaaa atagaagaga
     3001 gattaaagtt agatagtgat gatgattatg attattgagg tttaaatgac taaaaagatg
     3061 gaaaaaatag aagatagggt ttcataccac caaaaacagc ttgctcaact aaaagcaaag
     3121 aaaaaagagg agttgagtaa agaaaaaaaa gcagagaaag caaaaagagc aagtaaaaat
     3181 attttgcttg gtgcttctat ctgtaaagtg ttaggtattg atcacactga aatagatgaa
     3241 tatttaccta aaatagtagg tctgttttct agtacggttc aatatataaa tgatccaaag
     3301 gtattggcta aaagaactga tcttttagag agctggaaaa atggcggcga cagaaacgag
     3361 taaaacttgc ttttgctcgt ttgctggcgt ctatgcagtt agaaatgttc ataaaatcaa
     3421 cggagtatag tatatattag tatagtatat actgccctcc gggatagctg gccagcttcg
     3481 taaccggggg gtttgtttgg gggagttggt cagtcgtccc tatcgagctt cagctttttg
     3541 aagagatcag gggcatcagt agctgcaaaa acgtctgtac gggcttctga gcgttcaatc
     3601 gttgatttgg tgactgggtt tacatgaaaa agcgttctac gagctgcagg acgtttacct
     3661 tgatggattt ctacagcttc ctgtgcagat tccatcaggt cacggaaaaa ctggctactg
     3721 acttcctgat ccatctcttc gagctgcttt tggtcaggga tagagccttg accacgtaaa
     3781 aagccgagct tgcgcttatc tttcatatcc tgcgtatctc cactatccag cacagccaga
     3841 tcttactctc tattttatca gccttcggcc ttattggttt attggaatgg gtttccagta
     3901 gtgcaggaaa actgcccctt tacagggctg ttatctgata tggttaagac gcacacttga
     3961 caaact
//
