LOCUS       NZ_CP058201             4509 bp    DNA     circular CON 08-APR-2024
DEFINITION  Enterobacter roggenkampii strain RHBSTW-00002 plasmid
            pRHBSTW-00002_6, complete sequence.
ACCESSION   NZ_CP058201
VERSION     NZ_CP058201.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148444
            Assembly: GCF_013635755.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00002
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95.537547253764x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013635755.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 10:12:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,771
            CDSs (total)                      :: 4,659
            Genes (coding)                    :: 4,452
            CDSs (with protein)               :: 4,452
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 63 of 207
            Pseudo Genes (incomplete)         :: 146 of 207
            Pseudo Genes (internal stop)      :: 28 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            Pseudo Genes (short protein)      :: 1 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058201.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00002"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:1812935"
                     /plasmid="pRHBSTW-00002_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(4377..4509,1..107)
                     /locus_tag="HV027_RS23480"
                     /old_locus_tag="HV027_23490"
     CDS             join(4377..4509,1..107)
                     /locus_tag="HV027_RS23480"
                     /old_locus_tag="HV027_23490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="WP_128312513.1"
                     /translation="MPVSAMRHKKTVSVTIEPALLHQAREAGINLSATLTTALKHQIRE
                     SEAERWKRANRKAIQELNRITNEHGLLSDDHRTF"
                     /name="type II toxin-antitoxin system CcdA family
                     antitoxin"
     gene            107..415
                     /locus_tag="HV027_RS23485"
                     /old_locus_tag="HV027_23495"
     CDS             107..415
                     /locus_tag="HV027_RS23485"
                     /old_locus_tag="HV027_23495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="WP_094309572.1"
                     /translation="MQFMVYRNNSNNRAYPYLLDVQSDIIDELNTRLVIPLFLLDEVNG
                     RPARRLTPVLNVDGEDFLVMTHEMASVRQSQLGEEVVSVQEHRQAIKNALDFLLDGF"
                     /name="CcdB family protein"
     gene            complement(519..755)
                     /locus_tag="HV027_RS23490"
                     /old_locus_tag="HV027_23500"
     CDS             complement(519..755)
                     /locus_tag="HV027_RS23490"
                     /old_locus_tag="HV027_23500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_037427059.1"
                     /translation="MTETEKLLLSALERLQDDYETRQQAWQASYDSLQTMFTTTSAALM
                     RSDQACQHLSGQVESLRQQVMSLTEAVSRLTRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(816..1301)
                     /locus_tag="HV027_RS23495"
                     /old_locus_tag="HV027_23505"
     CDS             complement(816..1301)
                     /locus_tag="HV027_RS23495"
                     /old_locus_tag="HV027_23505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_139592782.1"
                     /translation="MSGILDLAKAFEETSKQQAQRTGESVKSEFVKLEQSISEALKSSG
                     KNISDAIHEQNTRQTTLLRRSVTIALIWILVCGVLLIGILSGILWFQGTLIASRLDEMN
                     SYSQQLTDLKAKSGAGVEILTDDTRKNKYYVVLPKSAKGVELYTSKNDKQVVTYTAK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1345..1770)
                     /locus_tag="HV027_RS23825"
                     /pseudo=""
     CDS             complement(<1345..1770)
                     /locus_tag="HV027_RS23825"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1970..2305)
                     /gene="mobC"
                     /locus_tag="HV027_RS23505"
                     /old_locus_tag="HV027_23515"
     CDS             complement(1970..2305)
                     /gene="mobC"
                     /locus_tag="HV027_RS23505"
                     /old_locus_tag="HV027_23515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_080202507.1"
                     /translation="MDKRTKEIKIRLTESEHERLLARCDRAHLAEWLRSLGLGEHVLRR
                     RRVPDVDPALLRQVTGIGNNLNQIARKLHGLDAIPAYQQVSLLAVLTSLDRQLSELLET
                     QRDRQNS"
                     /name="mobC"
     gene            2595..2990
                     /locus_tag="HV027_RS23510"
                     /old_locus_tag="HV027_23520"
     CDS             2595..2990
                     /locus_tag="HV027_RS23510"
                     /old_locus_tag="HV027_23520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128312516.1"
                     /translation="MKKAAVCLVVLSVSACGVSQQDARNLNYGPSPKNYAAEVVSLLKS
                     ELKDPDSVQIKSITKPRRGYASYGFGTKEYGWHVDVTYNAKNSYGGYTGYETRQYIYNG
                     GYSIPHTYEITFIDNKEYYCNNNCSEK"
                     /name="hypothetical protein"
     gene            complement(3033..3188)
                     /locus_tag="HV027_RS23515"
                     /old_locus_tag="HV027_23525"
     CDS             complement(3033..3188)
                     /locus_tag="HV027_RS23515"
                     /old_locus_tag="HV027_23525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181239571.1"
                     /translation="MKEKKHECSHSNLEKEYISGMATGDYKCVDCGAVGWGKNWPENEQ
                     DRKEDK"
                     /name="hypothetical protein"
     gene            complement(3169..4095)
                     /locus_tag="HV027_RS23520"
                     /old_locus_tag="HV027_23530"
     CDS             complement(3169..4095)
                     /locus_tag="HV027_RS23520"
                     /old_locus_tag="HV027_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_063448382.1"
                     /translation="MSNQALDLFNERLPRKPYFTDELQFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRPGAAIDWSDRNAPAPTLTIMNPENGHAHLLYALKTAVRTAPDGKIK
                     PLKYAAAVEHALCKKLDADTGYSGLICKNPNHNHWQLAVWQPELYTLDWLADSLDLSAA
                     NDKEIIPDYGLGRNCTLFDKTRKWAYRAIRQGWPDYEQWLQACYERASAYNLQFASPLD
                     DKEVKGIVKSIAKWTNKQFTPESFHEYVIRTHSKDIQSIRGTKGGAISKGGGRKIDPLS
                     VESLKPWNDLGVSRRTYYRMLYERKKT"
                     /name="replication initiation protein"
ORIGIN
        1 agtcagaagc ggagcgctgg aaacgcgcca accgcaaggc aatccaggag ctcaaccgta
       61 ttacgaacga gcacggctta ctctctgacg atcacaggac gttctgatgc agttcatggt
      121 ttaccgcaat aacagcaaca accgggctta tccctatctg ctcgatgtac agagcgatat
      181 tatcgacgaa ctgaataccc gcctggtgat cccgctgttt ctgctggatg aagtgaatgg
      241 acggcccgcc cgccgcctga ccccggtgct gaatgtggac ggtgaggatt ttctggtcat
      301 gacacatgaa atggccagcg ttcgccagtc acagctaggg gaagaggtgg tgagcgtcca
      361 ggagcaccgg caggccatca agaatgcgct ggattttctg ctcgacggtt tctaagttta
      421 cctttccatc ccgtgatcgt gtccccggct gcgtccgggg gcaaatgagc gcgttttctg
      481 tcgttcctgt ttctgcgcca gctccctgtc cagtctggct acctgcgcgt caagcggctg
      541 acggcttcgg ttaagctcat gacctgctgc cgcaagctct ccacctgccc gctcaagtgc
      601 tgacacgcct ggtcgctgcg catcagcgcg gcggaagtag tggtgaacat ggtctgcaag
      661 ctgtcgtaac tggcctgcca tgcctgctgt cgtgtctcgt aatcgtcctg taagcgctct
      721 aacgcactca gcagcagttt ttcggtctcg gtcatggatt tgcttgtcct tatcgctaaa
      781 aacgccgtat ggcgcttctg tggcaactga cggggctatt tcgcggtgta ggtcactacc
      841 tgtttgtcgt ttttcgaggt gtacagctcg acgcccttcg cgctcttcgg cagcaccacg
      901 taatacttgt tcttccgggt gtcgtccgtg agtatttcca cccccgcccc cgatttcgct
      961 ttcaggtcgg taagctgctg gctgtagctg ttcatctcgt ccagccgtga ggcgatgagt
     1021 gtcccctgaa accacagtat ccccgacaga ataccgatca gcagcacacc acaaaccagt
     1081 atccatatca gcgctatcgt gaccgagcgc cgcagcaggg ttgtctgccg ggtgttctgc
     1141 tcgtggatag cgtcgctgat gttcttcccg cttgatttca aggcctcgct gatggattgc
     1201 tcgagcttca caaactcgct tttcacgctc tccccggtac gctgtgcctg ctgcttcgag
     1261 gtctcctcga aggctttcgc taaatctaaa atcccgctca tataacgctc cttttaaccg
     1321 gatatttcgt ccaccgtccg ggtcggcaat gctgagactg cttttcgttt ctcgcaccac
     1381 tgtcaacccg ccgttttcca gcgccctgac cacatcctca cggtcgcgga tcatcccctg
     1441 tgccaccatc gtttccagcg agtccgtaat ggtctgcgcc gccttctgac ggtcgccggg
     1501 aaggtcattt cccggtgtca gggttcgcct tcgggcggga tcatgagggt catcaagatt
     1561 caggcggtca ttggtcagag tctgccaggc gttgaccctg ggacggtcag cccggtcata
     1621 gtagggttgc aggcgtttcc cgctctgcaa ttcgatgttc gggatcacaa aattcagctc
     1681 cagcctgtcc ttgtcccggt gctccaccca aaggcaggcg tactggtcgc catccagacc
     1741 gggcaacagt gtctgctcga actcctccat cagacgctct ttctcatcgt cagggaggtt
     1801 ttcttcccgg aacgacaaca ccccggaggt gtaggcgcgg gcaaaatcac aaccatcaat
     1861 cagttgcctg atacgctcag gctcaccgcg cagcaccacc gctttttccc tgtctcgttc
     1921 tttccccagc aggtaatcca ccgggccact gcctgcaccc gcaccccggc tatgaatttt
     1981 gacgatcacg ctgtgtctcc agaagctcag ataactggcg atcaagactg gtcagtaccg
     2041 ccagaagaga gacctgctga taggccggga tagcgtccag accatgcagc ttacgggcta
     2101 tctgattaag gttatttccg atgccggtca cctggcgaag cagtgccgga tctacgtcag
     2161 gcacacgccg acgacgtaat acatgttcac ccaaaccgag cgagcgcagc cattcagcga
     2221 gatgagcacg atcacaacgt gccagtagtc gctcatgttc actctcagtc aggcggattt
     2281 ttatctcttt cgttcgcttg tccatgacac tccgctgaga aaactttcgc acccgagtgc
     2341 gaattttcgc agtggatgcc aggggtttcg gggggcggcg agccccctga accagtcaca
     2401 gaaggaaccc tcgcagaggg gacgctgtgt gtactggctt acggtatgtt gtagtatgtc
     2461 aggttctgtg ctcgctgcaa aaaaaaaagc cctcttctag agagggctgt tatctgatat
     2521 aggcttgttt tagtgccaaa gtgagttatc gttacgaccg gaaattataa gccagtgata
     2581 aaggacatct ttctatgaaa aaagcagcag tatgcttagt tgtgttatca gtttctgcct
     2641 gtggtgtatc tcaacaagat gctaggaatc taaattatgg tccgtcacca aaaaattatg
     2701 ccgccgaagt agtttcctta ttaaaaagcg aacttaaaga tcctgattct gtgcaaataa
     2761 aatcaataac aaaaccacgg cgtgggtatg caagttatgg atttggaact aaagaatatg
     2821 gctggcatgt agatgttaca tataatgcaa agaattcata tggaggctac acaggatatg
     2881 aaacaagaca atatatttac aacggtggtt attccatacc tcacacttat gaaattacat
     2941 tcatagataa taaagaatat tactgtaata ataattgctc tgaaaaataa aaaaacaagg
     3001 ctgccttaat attatcgcag ccttgtttta cgctacttat cttctttgcg atcctgctca
     3061 ttttcaggcc agtttttacc ccaaccgaca gcaccacaat caacacactt gtaatctcct
     3121 gtagccattc ctgaaatata ttctttctct aaattactat ggctacattc atgttttttt
     3181 tctttcatat aacatcctat aataagttct tcgactgaca ccaagatcat tccatggttt
     3241 taaagattcc acggataaag gatctatttt tctaccacca cctttgctaa tcgcccctcc
     3301 tttggtgcct cttatagatt gaatatcttt tgaatgagtt cttattacat actcgtgaaa
     3361 tgattcaggg gtaaactgtt tattagtcca cttagcaata cttttaacaa ttcctttcac
     3421 ctctttatca tcaagaggcg atgcaaattg aagattgtag gcgctggcac gctcatagca
     3481 ggcttgcagc cattgttcgt aatcaggcca gccctgacga atggcacggt aagcccactt
     3541 gcgggtttta tcgaacagag tgcagttacg gcctaaaccg taatcgggaa taatctcttt
     3601 gtcgtttgcc gcactcaggt cgagtgaatc cgctagccag tccagtgtgt agagttccgg
     3661 ctgccagacg gcaagctgcc agtgattgtg gttcggattc ttgcagatta gtcccgaata
     3721 gccagtatca gcgtccagtt ttttacacag cgcatgctca accgctgccg catacttcag
     3781 cggtttaatt ttgccgtcag gtgccgtgcg caccgctgtt ttcagcgcat acagtaagtg
     3841 ggcgtgcccg ttttccgggt tcatgatggt gagagtcggt gcaggagcgt tccggtcgct
     3901 ccagtctatc gccgcgccgg ggcggtcaac gtcaaaaccg agccagaaca tcgcgtgtgg
     3961 ctggttgaac tggatgtatt tcgcaagaat tgcgcgctct ttcccggcaa tgcggacgcc
     4021 gaactggagt tcatcggtaa aatagggttt gcggggaagc cgctcgttaa aaaggtcgag
     4081 tgcctggtta ctcaatctcg cctgcgctta tggcgggctg ttgtttcgca cggttcatgt
     4141 gctagtatcc ttcttaggtt ttgtgcctgc gccctcgtta atcatgtcct tccgccaagt
     4201 cgagcatgat tacgggggcg tcgtcttttc tgccgcttaa cgttacacac cccgccgtat
     4261 tttgtcacct gtcaccgtca gttattgcct gaagcaacag accattacct tccgggtaaa
     4321 aaacgcgtac acttaaaaca cgtgtcagat acacgtgtaa aaatcaggag atcatcatgc
     4381 ccgtatcagc gatgcgccat aaaaaaaccg tcagtgtgac aatcgagcct gcgcttttgc
     4441 atcaggcccg cgaagccggg attaatctgt ctgccacgct caccaccgcc ctgaaacatc
     4501 agatccgcg
//
