LOCUS       NZ_CP058199            11613 bp    DNA     circular CON 08-APR-2024
DEFINITION  Enterobacter roggenkampii strain RHBSTW-00002 plasmid
            pRHBSTW-00002_4, complete sequence.
ACCESSION   NZ_CP058199
VERSION     NZ_CP058199.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148444
            Assembly: GCF_013635755.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 11613)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 11613)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00002
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95.537547253764x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013635755.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 10:12:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,771
            CDSs (total)                      :: 4,659
            Genes (coding)                    :: 4,452
            CDSs (with protein)               :: 4,452
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 63 of 207
            Pseudo Genes (incomplete)         :: 146 of 207
            Pseudo Genes (internal stop)      :: 28 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            Pseudo Genes (short protein)      :: 1 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058199.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11613
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00002"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:1812935"
                     /plasmid="pRHBSTW-00002_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(195..440)
                     /locus_tag="HV027_RS23370"
                     /old_locus_tag="HV027_23380"
     CDS             complement(195..440)
                     /locus_tag="HV027_RS23370"
                     /old_locus_tag="HV027_23380"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181239568.1"
                     /translation="MAFENIPRRFQRWKVIMVPGAKSYESWDEMVTGENFSEETRMKTY
                     KKHRTKAIDRTLANQEYIHTMTEDGITVRYRLERWR"
                     /name="hypothetical protein"
     gene            complement(559..840)
                     /locus_tag="HV027_RS23375"
                     /old_locus_tag="HV027_23385"
     CDS             complement(559..840)
                     /locus_tag="HV027_RS23375"
                     /old_locus_tag="HV027_23385"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249415533.1"
                     /translation="MVLAKGTAAPSPGKYESRPGHVNHPTKEANMQSIQQTTHTRYPGL
                     SIEHGEIRQFGKPRHCAANRRGQYQTTLRDQAGRYRTVSIISLYAWEA"
                     /name="hypothetical protein"
     gene            complement(855..1430)
                     /locus_tag="HV027_RS23380"
                     /old_locus_tag="HV027_23390"
     CDS             complement(855..1430)
                     /locus_tag="HV027_RS23380"
                     /old_locus_tag="HV027_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151785.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_181239569.1"
                     /translation="MSRIYGYARVSTGEQTIAGQEKAIHDKYPGIKIVADEGISGTVPA
                     TQRKGFASLLEFAEPGDSIVVVAIDRIGRNARTTLDVCEELQKRQINLISLREGFEFGT
                     PTGKLMLTMIAAFAEMELSTLKERTKAGMAAAAAEGRNAGRPTKQTPENIGNVLLKHDA
                     GIPAARIARDLGISRGTVNRILQKHAKA"
                     /name="recombinase family protein"
     gene            1938..2339
                     /locus_tag="HV027_RS23385"
                     /old_locus_tag="HV027_23395"
     CDS             1938..2339
                     /locus_tag="HV027_RS23385"
                     /old_locus_tag="HV027_23395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181239570.1"
                     /translation="MIADGKAALRAGEPVSPDAGVASFLLPDIEAELVVAEAAEADVAR
                     ERDTLLQDADRRDAAAALALFNWAHSVRVQRIELLLKLAMDEATTLAETDGGRGLYRTV
                     IAPDRRLNQIFATQGAIEILRRNRGMEGV"
                     /name="hypothetical protein"
     gene            2522..2911
                     /locus_tag="HV027_RS23390"
                     /old_locus_tag="HV027_23400"
     CDS             2522..2911
                     /locus_tag="HV027_RS23390"
                     /old_locus_tag="HV027_23400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_149015768.1"
                     /translation="MNDVKGCKTLTKSTGADDFGLPPLSSVVETVVETGGAAQTFAPIG
                     APVASPTEAVHRARAQSIQDAAFLNEMKAAGVAPADLVLLDGVLTSSRGMPDAARAPAF
                     AEVRRQVQAGGMTLSPAARGIDWNA"
                     /name="hypothetical protein"
     gene            2904..3266
                     /locus_tag="HV027_RS23395"
                     /old_locus_tag="HV027_23405"
     CDS             2904..3266
                     /locus_tag="HV027_RS23395"
                     /old_locus_tag="HV027_23405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186829.1"
                     /translation="MRKLPRHLQGEVLMPLSSRITRSQRLELIWLLPVLRLHPGDRSVT
                     ERRALGRFRGSLAAGGQTDAFVRSDASLYALGLRFQRKRTRELVASDPDVAAAGEALEQ
                     RANGVNLSETFGGGDE"
                     /name="hypothetical protein"
     gene            3259..3444
                     /locus_tag="HV027_RS23400"
                     /old_locus_tag="HV027_23410"
     CDS             3259..3444
                     /locus_tag="HV027_RS23400"
                     /old_locus_tag="HV027_23410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162780107.1"
                     /translation="MSEFAVSRLVIPEVVIPDGVIAARRARGQAQRVAAARRAAERRAA
                     PTWRWVRREQPRSTGR"
                     /name="hypothetical protein"
     gene            complement(3464..4105)
                     /locus_tag="HV027_RS23405"
                     /old_locus_tag="HV027_23415"
     CDS             complement(3464..4105)
                     /locus_tag="HV027_RS23405"
                     /old_locus_tag="HV027_23415"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_145062807.1"
                     /translation="MTEPTRPILTIKRRNRTVYVPPEKATKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELDMPPEADRKAVRRILGIVTGTSSYQQS
                     LIDQCMRYDLDGNPTEPATPEEAENAKARLTEIKAFRRKARQAKGEKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(4102..5391)
                     /locus_tag="HV027_RS23410"
                     /old_locus_tag="HV027_23420"
     CDS             complement(4102..5391)
                     /locus_tag="HV027_RS23410"
                     /old_locus_tag="HV027_23420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172743607.1"
                     /translation="MNDDNDTNESTQPNPAALERVSATEKFPEQLPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCHEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTASVCSDCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFTQNETRSLAKIPV
                     ILPQREQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLTLSVLAAVGRWDYINDI
                     IRRIDRDRGDAATLIALADHIRTNRPERDL"
                     /name="hypothetical protein"
     gene            5747..5956
                     /locus_tag="HV027_RS23415"
                     /old_locus_tag="HV027_23425"
     CDS             5747..5956
                     /locus_tag="HV027_RS23415"
                     /old_locus_tag="HV027_23425"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186834.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGNPEIKRFVADQRRPGRAADGVR"
                     /name="hypothetical protein"
     gene            5943..7115
                     /locus_tag="HV027_RS23420"
                     /old_locus_tag="HV027_23430"
     CDS             5943..7115
                     /locus_tag="HV027_RS23420"
                     /old_locus_tag="HV027_23430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181239565.1"
                     /translation="MAFVDFDTTHLKEDPGLFWRAVSAAGAPADRDEWVRLCLALGCWA
                     SLCGEDGLAAIEAWSTRHGRGENRHRQQYRSFLKGDLDTFAAQAFLNFSGVEWRNGRAS
                     VQSDVDRAERLRLSREATAKAKAERDKKGDAAARAAVKLLAKGGDPASTPYWRKKTALA
                     AAGGVQLPALSTDLGVGGMFRSFDPRAYWSDCLLIPVYKFDAARQLYVDGLEAICGRCS
                     AEGSQLTGSKFSPPGTRKQGGFYPMPGFMDVLDGSPHAPYSPLFVCEGFATAFALHHLS
                     RGKVAVCAAFSIAGFGSCVASLRRFWPDVEVMLVPDAGEAYRLAHKAAYGDDREPVPTI
                     PGASVVSFDGLNRFEPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERG"
                     /name="hypothetical protein"
     gene            7373..7831
                     /locus_tag="HV027_RS23425"
                     /old_locus_tag="HV027_23435"
     CDS             7373..7831
                     /locus_tag="HV027_RS23425"
                     /old_locus_tag="HV027_23435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_148502021.1"
                     /translation="MKSKTELRAAATARALEVIASEMGGWSLDGFAHGSLPNFSPLPRQ
                     QTQEGSVVLERPPFDYTWAGTAAFTDRANRALQVKLPASRERNYIWLCAVEREAVATAL
                     MVESFNVTGCAAFAGLPPVDGMVLLTMDEADVELIRAAMLPWLDAAAA"
                     /name="hypothetical protein"
     gene            complement(7849..8352)
                     /locus_tag="HV027_RS23430"
                     /old_locus_tag="HV027_23440"
     CDS             complement(7849..8352)
                     /locus_tag="HV027_RS23430"
                     /old_locus_tag="HV027_23440"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181239566.1"
                     /translation="MRREKTKSFNVRLPVSLLARIESEALRKGLSRSDVVIEALSGVMG
                     IEQSGADTKLLTAIEALRAEVRALGNGSGVAPQSAQRQAKEDVSHTAKQKARQIVPRGA
                     DPRFQAVSDEADQLIVSMTGENATLPAIAQALNAAGHTTATGLEWNTSRVRDYRHKLRE
                     RGLI"
                     /name="hypothetical protein"
     gene            8874..10397
                     /locus_tag="HV027_RS23435"
                     /old_locus_tag="HV027_23445"
     CDS             8874..10397
                     /locus_tag="HV027_RS23435"
                     /old_locus_tag="HV027_23445"
                     /inference="COORDINATES: protein motif:HMM:NF017116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="WP_181239567.1"
                     /translation="MSNHEENSGAAVATVTQIAAAKAKAAAKTERPAFMGGSLPPVPFN
                     EWDGLTGNPTRDGFRIQAAEKRSINFGNEYPLRYVPPLISDAGTGRWDVLNHADNLRWL
                     LYRSGARMELNLMTFKNELSWADSGSLVEPDEHGARAWILNKSAEVGLKRQAVDDFMFY
                     VARSAPYHPVARLLGSREWDGSPRVDAVLDCVPVAPGKEALRSALLRGLLIAAITAVEE
                     GAVSNKYVPCLYSEANNWFKTAFFRALFDLLPGAFGQGVSINPSSKDSVRPAVMRWFNE
                     LGEVSGMMKKNDPDEIKAFVERNVDEWRPENEKAVIAKKRQMVMGATVNKRDYLKDETA
                     ASRFPTITLVAPIDIARVNELLGWEWDNVKGCAVRADEDQLIQFWLEVRHLYRSGVGFN
                     LDDETFAAAKAETAQFVDRGSYYEPVRDILLLPGNASTGQYPWDKPGYDGRFTATDVTA
                     ALRAPSSHSGRVGRALKRLADEGIVDRRNHRGTFVYWKLPDEPTGELPL"
                     /name="VapE domain-containing protein"
     gene            complement(11143..11610)
                     /locus_tag="HV027_RS23440"
                     /old_locus_tag="HV027_23450"
     CDS             complement(11143..11610)
                     /locus_tag="HV027_RS23440"
                     /old_locus_tag="HV027_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064186840.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATSATVARTMGIPPRTVRRWASEKA
                     AAPSNVLSIEEMRKRAAAAVEATPQAAIRRLKNHFVQQQFDLLQRHAKDLQALRSASLQ
                     AMLEKDATMVKAISGLMTALLKAQERERLIYEIKPGTEADIMREGMNRKQQ"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 tcctgctccg gttggtttct ctcccgtatt gaaacgtcac gcgccgaaaa tggcgggggt
       61 ttttgggggg tacacggcgt ttgtgcgtgc gtggtcgtgc ggttgggtgt ttttgtggct
      121 gaggcgctta gaatggctct ggtgcgttct ggtgaagggg gagggcgcag ccgtcgctgc
      181 gctcctggtt ggggttagcg ccagcgttcg aggcggtacc tgacggtgat cccatcctcc
      241 gtcatggtat ggatgtactc ctggtttgct aaggtgcggt cgatagcctt ggtgcggtgc
      301 tttttgtacg ttttcatcct ggtttcttcg gagaagttct cgcctgtaac catctcatcc
      361 catgactcgt atgacttcgc tccaggaacc atgattacct tccatctctg gaacctccgg
      421 ggaatgtttt caaatgccat tgttctctcc ttgtcattct ggtttctggg ggcgctttcg
      481 cgcccctgta agtggttcgt aatggtgtaa ggcttttgct attgcttgtt gcttacacct
      541 gtaaggcttt cgttatggtc acgcctccca tgcgtaaagg ctgatgatgc tgacggtgcg
      601 gtatcgccct gcctggtcgc ggagggtagt ctggtactgg cctcggcggt tggcggcgca
      661 atgacgcggc ttgccaaact ggcggatctc gccgtgttcg atggaaaggc ccgggtagcg
      721 ggtgtgtgtg gtctgctgta tgctctgcat gttagcttcc ttcgttgggt ggttaacgtg
      781 cccgggtcgg ctctcatact ttcccgggct gggtgcggcg gtgcctttag cgagtaccac
      841 cgccgcgctc tctcttacgc tttcgcgtgc ttctgtaaaa tcctgttgac cgttcccctg
      901 ctgatgccga ggtcgcgtgc tatgcgtgcg gcggggatcc ctgcgtcgtg cttgaggagg
      961 acgttgccga tgttctccgg cgtctgtttg gtcgggcgtc ctgcgttgcg tccctccgct
     1021 gctgctgctg ccattcctgc ctttgtgcgc tccttgaggg tggacagttc catctctgcg
     1081 aaggcggcga tcatggtgag catgagcttg cccgtcgggg tgccgaactc gaaaccttcc
     1141 cggaggctga tcaggttgat ctggcgcttc tgcagctcct cgcaaacgtc gagggtggtg
     1201 cgtgcgttcc ggcctatgcg gtcgatggcg acgaccacga tgctgtctcc cggttcggcg
     1261 aactccagca gggaggcaaa tccctttcgc tgggtggcgg gtaccgtgcc gctgatgccc
     1321 tcgtcagcga cgatcttgat gcccgggtac ttgtcgtgga tcgctttctc ctgcccggcg
     1381 atggtctgct cgccagttga aacgcgggcg tagccgtaaa ttctgctcat gttgtctccg
     1441 tggtgctcaa aatggtcttt gaacatattt gatcactttt gcacaaaaat caatgactaa
     1501 aaaccagaaa cgcctagagc cacgggggct gctcagaatc gctccttttt ggacgtgatc
     1561 accccgcgct gggcgagagc cctgcgccct ctgcgttgat gtctgcgctg cgtcgtgacg
     1621 gcatgctgtc tgagcggcgt ttcttgccgc tttgctgata acttgctgag aaccaaacca
     1681 tgaccgaaat taagaaagag agcgccgacg gcgcttggct acgctctacc cttgccgaat
     1741 tggcgggtga tgcgttccct gtgtatgacc tcccgtccct gcgggtagac ccgaactcct
     1801 ccactcagct ttcggcgctc gctgatcgtc aggctgcgcg tgaaatgcgt caggccgctt
     1861 ccgacgtaga ggcgcgacgg ctggacgccg ctcgagtggt ggaagggctc aagaccgagg
     1921 ccgaacggct gcggggcttg atcgccgacg gcaaggctgc gctgcgtgct ggcgagccgg
     1981 tgagccccga cgcgggtgtt gcgtcgttcc tgctgccgga catcgaggcc gagctggttg
     2041 ttgctgaggc tgctgaggcc gacgtggccc gggagcgtga caccctgctg caggatgccg
     2101 atcgccgtga cgcggcggct gcgctggcgc tgttcaactg ggcgcactcc gttcgcgtgc
     2161 agcgtatcga gctgcttctc aagctggcga tggatgaggc gacgacgctc gctgaaaccg
     2221 acggcgggcg cggcctgtac cgtaccgtga tcgcgcctga ccgccgtctt aaccaaattt
     2281 tcgccacgca gggcgctatt gaaatcctgc gccgtaaccg tggcatggag ggtgtgtaat
     2341 gtctgctttc aaactcctgt gtgaccggct tcgcaatgag gcgacgctgg caaaatctga
     2401 cggggccggc gctttcaaaa agcaatctct ggatcccgac ctgaagggcg gcaagaaggg
     2461 caaaaagccc gccggggagc tggaggagga cgaggcgatc gctgcactcc accgtaagcg
     2521 catgaacgac gttaagggct gcaagacgct gaccaaatcc accggcgctg atgacttcgg
     2581 cctccctccg ctgtcgtcag tggtcgagac agtggtggag acaggcggcg ctgcgcagac
     2641 tttcgcgccg atcggggctc cggtggcgtc tccgaccgag gcggttcacc gggcgcgagc
     2701 gcagtccatc caggacgcgg cgtttctgaa cgagatgaag gcggcgggtg tggcaccggc
     2761 tgacctggtg ctgctcgacg gcgtgctgac gtcctctcgc ggtatgccag acgcggcgcg
     2821 tgctccggcg tttgccgagg tgcgtcgtca ggttcaggcg ggtggcatga cgctgtcacc
     2881 ggctgcgcgt ggtattgact ggaatgcgta agctgccgcg tcacctgcag ggcgaggtgt
     2941 tgatgccgct gtccagcagg atcacgcgct cgcaacggct ggagctgatc tggctgttgc
     3001 ccgttctgcg gctccacccg ggcgatcgat cggtcactga gcggcgggcg cttggcaggt
     3061 tccggggatc gctcgctgcg ggtggccaga cggatgcttt cgtccgttcg gatgcgtctc
     3121 tgtacgcgct gggactgcgg ttccagcgca agcgcacccg ggagctggtt gcttcggatc
     3181 ccgatgttgc tgcggctggc gaggcgctgg agcagcgtgc aaacggcgtg aacctgtccg
     3241 agacgttcgg gggtggcgat gagtgaattt gctgtctcgc gcctggtcat tcctgaggtg
     3301 gtgatccccg acggggtcat tgctgcccga cgggcaaggg gacaggctca acgtgtcgca
     3361 gctgctcgcc gtgctgccga gcgtcgtgct gcgcctacgt ggcgctgggt gagacgggaa
     3421 cagccccggt cgacagggcg gtgaaacggt gagacggggc gcgttatgcg cccttttttt
     3481 cgcccttcgc ctggcgtgcc ttgcgccgga atgccttgat ctctgtcagg cgggctttgg
     3541 cgttttcggc ctcctccggg gttgctggtt cggtggggtt gccgtccagg tcgtagcgca
     3601 tgcactggtc gatgaggctc tgctggtacg agctggtacc cgtgacaatg cccaggatgc
     3661 gacggacggc tttgcggtct gcctctggcg gcatgtccag ctctgcgcct cgggtcagga
     3721 tctgctcgat gatgcccttt gccatggggc gacggtcaac tccccgggtc agtaagtcag
     3781 gccagtgctc tttcaggaat gctttggtca cccggttggc cagcgccttt ttggacttgt
     3841 tggcgatcgc gatgtcctgc tggcggcggt cgttctcctc ctgtctgcgg atcccctcgg
     3901 cgacctgctc gggcgtcttt ttcggctttg ccgctgtggg cttctgtgcg ggcttctgcg
     3961 gggctttcgc ttttggctgt accttcgcct tgctggtagt ccgttcggct ggctgtggct
     4021 cagatttcgt ggccttttcc ggcgggacgt agaccgtgcg gttgcgtcgc ttgatggtca
     4081 ggatcgggcg ggtgggttcg gtcataaatc tctctcaggt ctgttggtcc ggatgtggtc
     4141 ggcaagggcg atcagggtgg cggcgtcgcc gcggtcccga tcgatgcgcc ggatgatgtc
     4201 gttgatgtag tcccagcgtc cgacggcggc aaggacggaa agggtgaggc ggttgaaccg
     4261 actctctggt acccggtcga gggtcagggg gatgcgcttg cgggggcgtc ctggaccgcg
     4321 ccctgtccct ggtcttttga gcagctgcgg ctgctccctc tgcggcaaaa taacggggat
     4381 cttcgccagg ctgcgcgtct cgttctgcgt gaactcgggc accgcgatgt gctcgtctgt
     4441 ccatgtcctg cctccgggcc tggctgatgc gctggagtcg caggacgggc aggagacgac
     4501 gatctcccgt cgcggcttgc ccttgaccgg tgccttgctg atcaccaggg gcgcggcttt
     4561 gcaatcggag cagaccgacg ctgtccagac cgcgttgtct ggcagggtgg cgttccggta
     4621 ctggtgcgcg gcgtcatgcg gtgtctggaa gctgaccgtc tgaaacgtgg gggcgacgct
     4681 gaggggtgcc gcccatggct ggcacctggg acaagcaagc tccacctctc cggcgtgccc
     4741 gtcgccaccg ttccagcgca ggggctcgcg gtgttcgtgg cagtagtaca cgaaaccgtc
     4801 gcggcgtgtt tgcccgaccg ggcctggtcg gcgatcgtgg aaccgtgcgc ccgggttggc
     4861 tcgcatggcg cggacgagcg cctcgccctg gtggatgccg taccagacct gccagccatc
     4921 gccgatgccg atcaggaacg gcgagacgaa cggtggggtg cggttgactc tcccgcgtgc
     4981 gttggctccg atggtggctt cgcgttcgcg gttccagcag gagacgcaca ggctcgaacc
     5041 gggcttattg ggggcgatca ggcggttgtc tttccggctg caacgggggc agagcttacg
     5101 ggtgtctgcc ggggtggtca gtggtgcgtc agggggctgt tcggtaccga cccaaagcgc
     5161 gaggccagtg gggcaccggt ggcactcgag gtgggggttt tcaatcgtgc gatcgaggtg
     5221 gctctgctca taaatctgtc ggcatgcctc gacccggagg gagcacccgc cggggcaggt
     5281 gacgaacaaa acgccgggga gctgctcggg gaatttttca gtcgcgctta cgcgttcaag
     5341 tgctgccggg ttcggctgcg tcgattcgtt cgtgtcgtta tcgtcgttca ctgctccccc
     5401 tgcgtcgaaa tggtcgccag acagcgccag cactggctgt gcgtcttttt tagtatctca
     5461 caaaaaacgc cactttggcg ttaattgtgt tacgcttgtc ttactgattc ctgacgagca
     5521 gttgggaaac atgaaaccgg atcgcccggt gccgctttcc ctcctcccca acgaggcggg
     5581 gttgtaaacg aggatgcgcg cttcgtcctt ggcaggtgcg agtaagcgag aagggctgct
     5641 gtcctgccgc tatgcggtaa gtcctcaagg gcggctttat cgataatcgt cactcccgcc
     5701 ccccgatgcc cgttaagctg aggggggcac ctatctggta ctcccaatga aaaaacgaat
     5761 ctataccgct gacctcgctg cgctgctgaa cgtgtcgctg cgcacgctct cgcggctaag
     5821 ttcgcggggg atcgtgccga ctccggcagg gcgctccctc cgtggcgggc attactggct
     5881 ggctggcaat cccgagatca aacgcttcgt tgccgaccag cgtcgtccgg ggagggctgc
     5941 tgatggcgtt cgttgatttc gacactaccc atctgaaaga ggatccgggc ctgttctggc
     6001 gtgccgtatc ggctgcaggt gctccggctg atcgagacga atgggtgcgt ctctgcctgg
     6061 ctcttggttg ctgggcgtct ctctgcggcg aggacgggct ggcagctatc gaggcctggt
     6121 ccactcgaca tgggcgaggc gagaatcgcc atcgccagca gtacaggtcg tttttaaaag
     6181 gcgacctgga tacattcgcc gctcaggctt tcctcaattt ctccggcgtc gaatggcgca
     6241 acggtcgcgc ttctgtccag tccgacgtcg atcgcgctga acggctgcgg ttgtcccgtg
     6301 aggccacagc gaaggcaaag gctgaacgcg acaagaaggg tgatgctgct gcgcgtgctg
     6361 cggtcaagct gctggcgaag ggaggagatc ctgcgtctac cccatactgg cgcaaaaaga
     6421 ccgctctggc ggctgcgggt ggcgtccagc tgcccgccct cagcacagat cttggtgttg
     6481 gcgggatgtt tcgatcgttc gatccgcgtg cttactggtc tgattgcctc ctgattccgg
     6541 tttacaagtt cgacgccgcc cgtcagctgt atgttgacgg cctggaggcc atctgcgggc
     6601 gctgctcggc tgagggttcc cagctcactg gctcgaagtt ctctccgccg ggtacccgca
     6661 agcagggcgg cttctacccg atgccggggt tcatggacgt gctcgacggc tccccgcacg
     6721 cgccctactc gcctctgttc gtctgtgagg ggttcgctac ggcgttcgcc ctgcaccacc
     6781 tgagccgtgg caaggtggcg gtctgcgctg cgttctcgat cgccgggttc ggcagctgcg
     6841 tggcgtcgct gcgtcggttc tggcctgatg ttgaggtgat gctggtaccg gacgcgggcg
     6901 aggcgtaccg gctggcgcat aaagcggcct atggcgacga tcgcgagccg gtcccgacaa
     6961 ttccaggggc gtcggtggtg tcgttcgacg gcctgaaccg cttcgagccc cgcgataacc
     7021 tcgactggtt cgacgtgctg gttgaggagg gcgaggatcg ggcgcgtgag ctgctccggt
     7081 atgcggtgcg tcatgcccgg cttgagcggg gctgatggct gggctttttt gcgtcggttt
     7141 cctcgggagg ccgctgaccg taaagggtcg ttttctggct cgtctttttt gcatcgacgc
     7201 gctggcttct gaggcatggt taaagacgca ctaccgcgac tttttcgact cgccacgttt
     7261 taggcgcact ttccgtcgtg acgctatcct gcagctggtt tggtttccag cagcacactc
     7321 aagcccggca ccgttttggt gtcgggcttt ttttcgcccg taaggagggc acatgaaatc
     7381 aaagactgaa ttacgcgctg ccgcgacggc tcgcgccctt gaggtgatcg cttctgagat
     7441 ggggggttgg tcgctcgacg ggtttgctca cggctcgctg ccgaacttct ccccgctgcc
     7501 gcgccagcag acgcaggagg gctctgtggt gctcgaacgc ccgccgttcg actacacctg
     7561 ggcgggtacc gctgcgttta ccgatcgtgc aaaccgtgcg ctgcaggtga agctaccggc
     7621 gtcgcgtgag cgtaactaca tctggctgtg cgcggtcgaa agggaggctg tggccaccgc
     7681 gctgatggtg gagtcgttca acgtgaccgg ctgcgcggcg ttcgccgggt tgcctcctgt
     7741 ggatggcatg gtgctgctga ccatggacga ggccgacgtg gagctgatca gggcggcgat
     7801 gctgccgtgg ctggatgcgg cggcggcgtg agccgtcgcc cctctgcgtc aaatcaggcc
     7861 gcgctcgcgc agcttgtggc ggtagtcccg gacgcggctg gtattccact ccagcccggt
     7921 ggcggtggtg tgcccggctg cgttcagcgc ctgggcgatc gcgggcaggg tggcgttctc
     7981 cccggtcatg ctgacgatga gctggtcggc ctcatcgctg acggcctgga agcggggatc
     8041 ggctccccgg gggactatct gtctcgcttt ttgttttgca gtgtgagaaa catcttcctt
     8101 cgcttgtcgt tgtgctgatt gaggtgcaac gccacttcca tttcccagcg ccctgacctc
     8161 agctcgcagc gcctcgatgg cggtcaaaag tttggtgtca gcaccgctct gctcaatgcc
     8221 catgacgccg ctcagcgcct ctatgactac atcgcttctg gacaacccct tacgcaatgc
     8281 ctcggactca atacgcgcaa ggaggctaac cggcaagcgg acgttgaatg atttcgtttt
     8341 ttccctgcgc atgttgacct cttgatttgc tgttgcgggt gctttttgtt tttcgttctg
     8401 ctctcaaatt gtacgtcgtt atgttgtgca tgtcgatttc gacgtcttcc gcgcagaggg
     8461 gcgacggcgt ggcgtcgcct tgggtgcctg tggacagttt ctgtggacaa gtattttgcg
     8521 gcggggctgg cccgtggata agtcgcggag gctgcgcggc tctaagatcg gcgcgggatc
     8581 gcgtttatcg ctgcgggatc gctatgtact ggcgcagggg gtgggtgtaa cggtatgatg
     8641 gcgcggctga aaaagaaaaa cccgccttgg ggagacgggt tttcttcaaa gctgttttga
     8701 gtttggagct ttcacggcgg tcaccgtgga aaacacataa taactcatgt tgggtgtgaa
     8761 gccagcgtga attatacgcg atcataatga cgcgtgcaaa gggtgttttc ctcgtaaagt
     8821 cccgccgttg taaggctcct tgagattggc tgttcaattt tgaggacgta aaattgtcaa
     8881 accatgaaga aaattctggc gctgctgtcg ccaccgtaac gcagatcgcc gccgccaagg
     8941 ccaaagctgc cgccaagacc gagcgccctg cgttcatggg tggttctctg cctcctgtcc
     9001 ctttcaatga gtgggatggg ctaaccggta acccgacccg cgatggtttc cgcattcagg
     9061 cggctgaaaa gcgatcgata aatttcggca atgagtaccc gctgcggtac gtcccgccgc
     9121 tgatctctga tgctggcact gggcgctggg acgttctcaa tcacgccgac aatctccgct
     9181 ggttgctcta ccgcagtggt gcccggatgg agcttaacct catgaccttc aagaatgagc
     9241 tctcatgggc tgatagcggc tctctggtcg aaccggacga gcatggcgct cgcgcctgga
     9301 tactgaacaa aagcgccgag gttggcctga aacgtcaggc tgttgacgat ttcatgttct
     9361 acgttgcccg ttccgctcct taccaccctg ttgcccgcct cctcggttcc cgcgagtggg
     9421 acggttctcc tcgcgtcgat gccgtgctgg actgcgtgcc tgtcgcacct ggtaaggagg
     9481 cgctgcgctc ggctctgctg cgtggactgc tgattgcggc gatcaccgct gttgaggagg
     9541 gggcggtgtc gaacaagtac gttccttgcc tctactccga ggctaacaac tggtttaaga
     9601 cggcgttctt tcgcgctctg ttcgacctgc tgcctggtgc gttcgggcag ggtgtgtcta
     9661 tcaacccttc gagtaaggac agcgtgcgcc ctgcggtcat gcgctggttt aacgagctgg
     9721 gtgaagtgtc gggcatgatg aagaaaaacg accccgatga aatcaaggcg ttcgtggagc
     9781 gcaacgtgga tgagtggcgt cctgaaaacg agaaggctgt gatcgccaaa aagcgccaga
     9841 tggtgatggg tgccacggtg aacaagcgcg actacctgaa ggacgagacg gcggcgtccc
     9901 gcttcccgac cattaccctc gtcgccccta tcgacattgc tcgcgttaac gagctgctcg
     9961 ggtgggagtg ggataacgtc aaaggctgcg ctgttcgggc ggatgaggat cagctgatcc
    10021 agttctggct cgaggttcgt cacctgtacc gctctggcgt cggtttcaac ctggacgacg
    10081 agacctttgc tgcggcgaaa gctgagacgg ctcaattcgt cgatcgcggc tcgtactatg
    10141 aaccggtgcg cgacatcctc ctattgccgg gcaatgcgtc tactggccag tacccgtggg
    10201 acaagcctgg ctatgacggc aggtttaccg cgacggacgt gacggcggcg ctgcgtgccc
    10261 catcaagcca ttctggtcgc gtcggtaggg cgttgaagcg cctcgctgat gaggggatcg
    10321 ttgatcgcag gaaccaccgg ggtacttttg tttactggaa attgccagac gaaccgaccg
    10381 gggaactccc gctctgaaaa tgacccgctt cggcgggttt ttttatgcct ctgccatgtt
    10441 ggggtacccg tgtgggggtt ccgttttgtg tgggggtatg gggtgatcgt gtgggggttc
    10501 ggtgtggggg taacgtgtgg gggtaattgg tcgtgtgggg gtaaaatgtg ggggtaaaat
    10561 cgcaaagaaa atctaccccc acacgctcta aatcccttta atggcggtgc ttgtggattt
    10621 agtgttgggg tatgttgggg tacatttttt tatttatcta ctatgtaaaa aaaagggtaa
    10681 taagtagcac ccacaccccc tgtatgccca tgggttaccc ctacaccctc atacccccac
    10741 acgctcaaaa attgcgcagt tgacgcgccg cagctgcacc ccttcgggga tgcactttgc
    10801 ttgtcttggt cgatcggtag ataggttcgt cgtgccgggg tggtgggtgg tagtggttag
    10861 ggggtggatc gggtggttgg ggtggcgctg agggcacgag ctgtcgctcg cgctgggtgg
    10921 tcgtctgttg gttctgcgtg agggcttgcc ctcgcgccct ctgcgttatg ggtggaatgc
    10981 gtagctgcag ctgcgcgttt ggttcgtccg ggcatcaaaa cggggcttct cgcgcacgcg
    11041 agcggtaaca attgcgtaac gtgcggatcc gcgtattccg tgggcttccg ggttttggcg
    11101 gtcacaaaaa cggtcacatg gcggtcattt cggtcatggc ggtcattgtt gtttgcggtt
    11161 catgccctcg cgcatgatgt cggcctcggt gcccggcttg atctcgtaga tgaggcgttc
    11221 gcgttcctgc gctttgagca gggcggtcat gaggccgctg atcgctttga ccatggtggc
    11281 gtccttctcc agcatggcct gtaacgatgc gctgcggagg gcttgcaagt cctttgcgtg
    11341 gcgctgtagc aggtcaaatt gctgctgtac aaagtggttt ttaaggcgtc ggatcgcggc
    11401 ctgtggtgtg gcctcgacgg cggcggcggc gcgtttccgc atctcctcga tgctgaggac
    11461 gttgctgggt gctgcggcct tctcgctcgc ccatcggcga acggtgcggg gtgggatccc
    11521 catggtgcgt gcgacggtgg cactggttgc gccgtcctgc tccagtagcc tgagcgcctg
    11581 ggctttctgt tcggtggtgt atttcttcat ggt
//
