LOCUS       NZ_CP058195             2495 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00012 plasmid pRHBSTW-00012_5,
            complete sequence.
ACCESSION   NZ_CP058195
VERSION     NZ_CP058195.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148450
            Assembly: GCF_013635495.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00012
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 82.1581862133776x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013635495.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:58:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,924
            CDSs (total)                      :: 4,810
            Genes (coding)                    :: 4,695
            CDSs (with protein)               :: 4,695
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 39 of 115
            Pseudo Genes (incomplete)         :: 80 of 115
            Pseudo Genes (internal stop)      :: 20 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            Pseudo Genes (short protein)      :: 1 of 115
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058195.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00012"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00012_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            342..527
                     /locus_tag="HV033_RS24495"
                     /old_locus_tag="HV033_24505"
     CDS             342..527
                     /locus_tag="HV033_RS24495"
                     /old_locus_tag="HV033_24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(818..1036)
                     /locus_tag="HV033_RS24500"
                     /old_locus_tag="HV033_24510"
     CDS             complement(818..1036)
                     /locus_tag="HV033_RS24500"
                     /old_locus_tag="HV033_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            2244..2471
                     /locus_tag="HV033_RS24505"
                     /old_locus_tag="HV033_24515"
     CDS             2244..2471
                     /locus_tag="HV033_RS24505"
                     /old_locus_tag="HV033_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          complement(575..662)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          1301..1829
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 aatgtggcct ttttctttac ctgtggttct ccgccaaaat cccagcaaat tgcatcacca
       61 aagctaaaag ctttcagggt tgtctaaaaa gcacaagaca taagaggaag tgcggtattt
      121 tataatcaag cccccaagaa tttaccataa catccgttgc ccgcaccgcc tgagacgcgt
      181 tcagcgcgtt cctgacgaaa ccatgacaaa aaccacaaca aaccaccccg gaacccgtca
      241 gaaacgcgcc tgttaaattt taacggcatg catgactatg caccagaatg acgccatgct
      301 cttttcacgc aaaaatcatc accagacggg gaaaatcacc agtgaccaga caggaatccg
      361 ccgccctcaa tatggccaaa tttatccgcg cacagacact tctcctcctt gagcggctcg
      421 agcagatgga tctggatgag gctgccggct gctgtgagca cctgcacgat caggccgaag
      481 cgctttacgc catgctgaac gcacagatag gcgaggaaaa tgcgtgaaaa tcggtgaacg
      541 ggtgcgcaat tcagtgcgcg gccgtgaggc gatggcgggg tgtcggggcg cagccctgac
      601 cagggtattt gtgatgccgg cgcgtgcgcg gtattacaaa tgcacatcct gtcccggaac
      661 ggacaccggg aaacagcaaa aaaaaccggg cggcacgccc ggaactcaat caagttagat
      721 tagattactc tcactcgtcc ataacagcat catggaacga cgaccaccgt ccgtgacggc
      781 cgcctcgttt aagtatggac agaaatacag aaaatgctca ggacgaaatg taatgaatgc
      841 gaacggattc aagaaattcg agcatgacag tccttacggc cggttcggtt tcagacaaaa
      901 tctgccggta tgcatccagc atcatggctc cggcatcccc tccggcacgc cgtagccaga
      961 ccgaaacaac ggacacaagc aggtgtcgct catcatcact aagagtcatc agggctccgg
     1021 aagaaaaacc aaacatggct tacctactgt taacggcaac gcagaagaaa ggtttacagg
     1081 gtagcgggca gggatgaaaa aaaaaaaatt gcagtcaggc cggaacggct gctgaaccgg
     1141 tacaggcggg ttccgggcgc agcgccggac cagtcacgga gcactagcgg agtgtatact
     1201 ggcttaagat gtagcatggc agtaacacat agcattaatg ccacataaga caggcgcaga
     1261 aagtgaaaga aaacgcgtca ggagaaaatg tgatacagaa tatattccgc ttcctcgctc
     1321 actgactcgc tccgctcggt cgttcggctg cggcgagcgg gacgggctta cggatggatc
     1381 agaaatttcc tgggggatgc caggaagata cttaacgaag aaagcataag gccgcgccaa
     1441 agccgttttt ccataggctc cgcccccctg acaagcatca cagaatctga cgctcaaatc
     1501 agtggtggcg aaacccgatg cggcttaaag ataccaggcg tttccccctg gtggctccct
     1561 cgtgctcccc cgcattcctg cctttcggtt taccggtgtc actccgctgt tatggccgcg
     1621 gtttctcatt ccacgcctga cactcagttc cgggaaggca gttcgctcca ggatggactg
     1681 tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcaacttga
     1741 gtccaacccg gaaagacacg actaatcgcc attggcaacg gccattggca aacaggatgt
     1801 gcgagagatg tctcaagagt tcttgaagtg gcggccttag tgaggctaca ctgaaaggac
     1861 aaatttggtg acatgtctcg cacaagactg ttaccacggt taggggatct gccaggatta
     1921 cctaaccttc gaaaaaccac ctgccagggt ggttttttcg tttacagggc aagagattac
     1981 gcgcggaaaa aaaggatctc aagaagatcc tttataaatt agacatccaa cctcacaaag
     2041 aggaatactt aaatcatctt ggtatatgta tcggccggat tacatctcat ataagcctcc
     2101 tccccgcctg acaccacact ttgaccatag cacctcgcgc ctaactcgat atacagccca
     2161 ccatcaagcc tcttttcaca taaaaaatga cctatagtta gatggttagt gaccaaaact
     2221 gatatggata aaaggagctg gctttgaaaa agatttttgt ttcagtgttc gcagcggcag
     2281 ttgcgctttc agccctcacc ggctgcacgc gcacaagcta tgccatacac accaatgacg
     2341 gtcgcactat tgtcagcgac ggcaaaccta aagaatcaga ctcaggcctg cttggctata
     2401 cagacgcgaa tggtgtcaaa cagcagatca ataaaaccga cgttaaagaa gtctccgaaa
     2461 ttccgcactg agcatcttct ccgaaaaagg ccgct
//
