LOCUS       NZ_CP058194             4161 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00012 plasmid pRHBSTW-00012_4,
            complete sequence.
ACCESSION   NZ_CP058194
VERSION     NZ_CP058194.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148450
            Assembly: GCF_013635495.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00012
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 82.1581862133776x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013635495.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:58:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,924
            CDSs (total)                      :: 4,810
            Genes (coding)                    :: 4,695
            CDSs (with protein)               :: 4,695
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 39 of 115
            Pseudo Genes (incomplete)         :: 80 of 115
            Pseudo Genes (internal stop)      :: 20 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            Pseudo Genes (short protein)      :: 1 of 115
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058194.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00012"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00012_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            357..737
                     /locus_tag="HV033_RS24470"
                     /old_locus_tag="HV033_24480"
     CDS             357..737
                     /locus_tag="HV033_RS24470"
                     /old_locus_tag="HV033_24480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_213070177.1"
                     /translation="MEYRGLDFEEQEFHDCLNILHKEIVSFLNENNMAMDFFQYPVEDI
                     CRIIFRDKIDLRTAVILDFGITTYDLSPLADHDPFVWRLLEWPLYDYENFQFVIVQKLA
                     SYELDKLNEIIQVEDNKDKRRL"
                     /name="hypothetical protein"
     gene            739..1008
                     /locus_tag="HV033_RS24475"
                     /old_locus_tag="HV033_24485"
     CDS             739..1008
                     /locus_tag="HV033_RS24475"
                     /old_locus_tag="HV033_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_063153150.1"
                     /translation="MSKSTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LNIKPEEINKALVLGLLSKINELDEEEKRDFTRIGMDILQQWKS"
                     /name="conjugal transfer protein TraD"
     gene            complement(1252..1521)
                     /locus_tag="HV033_RS24480"
                     /old_locus_tag="HV033_24490"
     CDS             complement(1252..1521)
                     /locus_tag="HV033_RS24480"
                     /old_locus_tag="HV033_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="WP_001324694.1"
                     /translation="MRRSTIRKGLTQKEMAERFNISVKTVKKYTAEPREQYEQTAADRR
                     RQAYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPKNSL"
                     /name="response regulator transcription factor"
     gene            complement(1518..2375)
                     /locus_tag="HV033_RS24485"
                     /old_locus_tag="HV033_24495"
     CDS             complement(1518..2375)
                     /locus_tag="HV033_RS24485"
                     /old_locus_tag="HV033_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_063153153.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEQVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQKKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPQNPLQYKELKATARSIARWTWKNFDSHTFSLIQSSRGAKNKNKPKKSNVKEMLGFI
                     GGEK"
                     /name="replication initiation protein"
     gene            complement(2914..4107)
                     /gene="mobQ"
                     /locus_tag="HV033_RS24490"
                     /old_locus_tag="HV033_24500"
     CDS             complement(2914..4107)
                     /gene="mobQ"
                     /locus_tag="HV033_RS24490"
                     /old_locus_tag="HV033_24500"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_063153152.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYLDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQREWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANVTYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNLNKKKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="mobQ"
     Typing          complement(3098..4107)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.11"
                     /coverage="85.0"
                     /evalue="0.0"
ORIGIN
        1 cccatacgtc gcttgcgacg tgtaagtggg cattactaat attatttttt cggttgcacc
       61 tcaaaataat aaagtaataa acccaggctt cgcccaatat aaatatatta aaatatattt
      121 gactaaagta aataatggag gtaatgaata ataaaataaa taattaggag gttatatgga
      181 aaaagaaact ctcggtcctg atatacaagt caattggtgt gatcattgta aaaaaaacac
      241 tcgccatata tttagattta ctgaggagta ttcaaaagaa gagggaccaa ttacagaggt
      301 tgagtgtact aaatgtggta atacattcac tatcttatag tgtggaggtt tttaatatgg
      361 aatatagagg attagatttt gaagaacaag aatttcacga ctgtttaaat atattacaca
      421 aagagatagt gtctttttta aatgaaaata atatggctat ggattttttt caatatccag
      481 ttgaggatat atgcagaata atttttcgag ataaaataga tctgagaact gctgttattt
      541 tagattttgg aattacaacc tatgatttaa gccctttggc agatcacgat ccttttgttt
      601 ggcgtttgct tgaatggcca ttatatgatt atgagaattt ccagtttgtg attgttcaaa
      661 aactggcttc ttatgaatta gataaattaa atgaaataat ccaagtagaa gataataaag
      721 ataaaaggag gttgtaatat gagcaaatca acagcacagc agatcgcaga tctacaaaat
      781 aaactacaag cattaagaac aaaaaaagca aggcaggaca gaagggaaga aacaagacaa
      841 aaaataatcc tgggtgcaga agtggctaag gttttaaata ttaaaccaga agaaataaat
      901 aaagctcttg tattaggctt gttaagtaaa atcaatgaat tagacgaaga agagaaaaga
      961 gattttacac ggatcggaat ggatatattg cagcaatgga aatcgtaaac cgtgaacagt
     1021 gaagaaccag ccgcctttgg ctggtttttc attgtgaagg gttgccagcc gtaggctgga
     1081 ggttttaagg ttgccagagg gcctctggtg gctgggtggg tttgttgatt tgttggtacc
     1141 agagtgtccc tcacctatcc agacacaagc ccggatacgg ggaaagggga aaaaatcccc
     1201 ccaggggtgg agggtttggt taggttaggg ggaaaaactg cttagaagtc gttacaggct
     1261 gtttttaggc atatctaagg caacataacg gcgatacagg gcaacggctc cgtgatacga
     1321 acagcccatt ttgtcggcta cctgttgcca tttcagcccc tggcttcgta gttcgtaagc
     1381 ctgacgacga cgatcagccg ctgtttgttc gtattgttca cgaggttcgg ctgtatattt
     1441 ttttaccgtt ttaacagata tattaaatct ttcagccatt tctttttgag ttaaaccttt
     1501 tcttattgtg cttcttctca tttttcacct cctataaaac caagcatttc tttaacatta
     1561 ctcttcttgg gtttgttttt atttttcgca cctcttgaag attgtattag tgaaaatgta
     1621 tggctatcaa aatttttcca agtccaacgg gctatgcttc ttgctgttgc ttttaactct
     1681 ttgtattgta acgggttttg tgggttgcaa aaagtattta aaccctgtgc ttttaatagt
     1741 agttcgtttt cgaatttctc gaaagtagat atttttttga aatttaaaac cgttctgtaa
     1801 gcatattggc gtagatcttc aaacaaatta acattgcgac ctaaaccata agcctcgctt
     1861 tttttctttt gtatttgagg cttttctaag tccacaaaat cagctaaata atccaggtca
     1921 taaaggtcag cacctgacca gaacggcgac cagtgatcat tgagtgggtt ttttgtaatc
     1981 aatccagcaa accccagatc tgctttgagt tttttcgcca tagccatttc aacggctgcg
     2041 gcatacttca acggcttcaa ccttgcttgt tgtgtcttac aaaccccggc tttcagcata
     2101 tagccgtagt gacaacgacc tgtctttggt gtttttgtaa taaagtgagg tatgggtaag
     2161 cccacctgct caaatgtata gaatgaattt ttcttatcta aatcaaagag taagcaagtt
     2221 tgaatggctg gctgatttgc ctgtaaatac agcatttcaa gggcttttgc cttgttcctt
     2281 atgcttaatc ccatatcgag gttatttgtg caataaggtt tgttaggcat ttgattttta
     2341 aaaaaatcga tgttatgttc ttcttgtatt atcattttta ttcagtcctt ttttatctgg
     2401 ccctgctcct ctgttctgtc gccaaaacaa tcacagaggg gtggggtttt tatttttata
     2461 gatttttcaa tcctgtacca gttctaaccg atcttaaaaa tttgtcaagt ggggagctta
     2521 accatatcag ataacagccc aacgaagttg gggcagttct ttacatacgg cagcaaagca
     2581 ttactatcag atctatggtg cgaagcatta ccgaagatag aaaaacaata aaaaaagaaa
     2641 aaagagtatt gttcgaagaa aaattggcga ttcgattttt ttaggtgaat atatgaaaaa
     2701 aaagaaagtg cagaaaaaac ggaaaagaaa taagcatagt aaatttttta aaatgcttat
     2761 gaagtcagtt aatgaagctg tggcaataca taaaggggaa atagaaccgg cgaggatcac
     2821 aactctaaga atgtagtata ttatagtata gtattaaata atattaagtt gtattaagtg
     2881 atactactat atgggcatat ataccactat atactacatt ccaaatttct tcttcctctt
     2941 aggttgctga tcttcaaatt ccggcattgg ttccggctca ggctcttcaa tttttggttg
     3001 tggttggttc ataggagaca ttccagagcc tgaacccatt tttccgcctc cattatttcc
     3061 accggttccg gttcctgtgc ctgttttagg ttttgtttct aactctgttt ttaatatatg
     3121 ttcgccaaat tcatttatta cataactact aacgaaattt tgaggatctt ctttttcttc
     3181 ttttttcttt ttatttaaat taggttttga tttaaatata tgctgggtct ttttgtataa
     3241 tgcctttaaa ttatcaatga ttgaccttgc aagaggcttt ttctctattt tcatagtttc
     3301 ctctggctga ggtggtttgg tttgctgcaa gtaggttaca ttagctattt ttaataataa
     3361 tgctttttct ttttgctcca tagctcttaa tttttgacct tttcgccatt ggtgcctgtg
     3421 tatcctggtc attggcgttc tgtccagttc aatggttttt gaaatccact tggctttctc
     3481 tttcggagtt ttagcaaaaa cagcctttct ttctgcctct tctttttggt cttttaatga
     3541 acgggagtca atacgagatt tatgaccata tttttttaat cggtcatttg ctattatttc
     3601 ccagttaact cgccaggctt caatgttttc tctattattc cattctcttt gtttgttccc
     3661 aaatccattt gggctaacag ttcttaaagt tagcattaca tgagcatgag ggttatgact
     3721 atcaagatca tgaaatgcta tgtctgcaat cattccataa tccacgaaat tttcttggca
     3781 gtaatcgagg acgatctgct tcttctggcc gtcgtccagc tccttgggaa tggctatgtc
     3841 cagataacgg catagttcgg catcctttcg cttctcgcca gcctctacgg cattccagag
     3901 ggtgcctgag tccttcacaa gttcggcagg tgtgccggca ggggagagga tagcagagcc
     3961 gaaaaatccc tctttttttg aataatccca ggttgccccg gttcgattat cttttgtctt
     4021 ttctcggttt tgatatgcag ataaataaac cgaagattta ccttctgatc ttcgtaattt
     4081 tttaaagctg atatgaaatg tcgccatagt ctacccctta ctttttttgt cttttgaatt
     4141 ttggttttta gccccgcagg g
//
