LOCUS       NZ_CP058193             6013 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00012 plasmid pRHBSTW-00012_3,
            complete sequence.
ACCESSION   NZ_CP058193
VERSION     NZ_CP058193.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148450
            Assembly: GCF_013635495.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00012
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 82.1581862133776x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013635495.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:58:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,924
            CDSs (total)                      :: 4,810
            Genes (coding)                    :: 4,695
            CDSs (with protein)               :: 4,695
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 39 of 115
            Pseudo Genes (incomplete)         :: 80 of 115
            Pseudo Genes (internal stop)      :: 20 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            Pseudo Genes (short protein)      :: 1 of 115
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058193.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00012"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00012_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            217..702
                     /locus_tag="HV033_RS24430"
                     /old_locus_tag="HV033_24440"
     CDS             217..702
                     /locus_tag="HV033_RS24430"
                     /old_locus_tag="HV033_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_181241453.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTEQSVKREFARLETSISEDLKSSS
                     QSISNAIREQNDQLTGIVRYSLLSALGWIFLFAALLIMILGGIIWYQGTVITSRLNEMD
                     SYSQQLESLKAQSGAGVAIYKDDKQKNTYLVVLPKKAHGIETYTSTAGNTVMKYTAK"
                     /name="MbeB family mobilization protein"
     gene            763..999
                     /locus_tag="HV033_RS24435"
                     /old_locus_tag="HV033_24445"
     CDS             763..999
                     /locus_tag="HV033_RS24435"
                     /old_locus_tag="HV033_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_181241455.1"
                     /translation="MTEMERQLLSALESLQTSYEQQQQAWQDSYSNLQRMFETTSQALE
                     RNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1070..1378)
                     /locus_tag="HV033_RS24440"
                     /old_locus_tag="HV033_24450"
     CDS             complement(1070..1378)
                     /locus_tag="HV033_RS24440"
                     /old_locus_tag="HV033_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="WP_181241457.1"
                     /translation="MQFTVYRNNGNSRAYPYLLDVQSDIIGELKTRLVIPLYSLNEFSG
                     RPARRLNPVLNVEDEDYLVMTHEMASVRLSQLGDEVVSVREHREVIKNALDFLLDGF"
                     /name="CcdB family protein"
     gene            complement(1381..1620)
                     /locus_tag="HV033_RS24445"
                     /old_locus_tag="HV033_24455"
     CDS             complement(1381..1620)
                     /locus_tag="HV033_RS24445"
                     /old_locus_tag="HV033_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="WP_181241458.1"
                     /translation="MTTSVMRHKKTVSVTLEPALLRQAREAGINLSATLATALRHEIRE
                     SESERWKRTNRKAIQELNRITDEHGLLSDDYRTF"
                     /name="type II toxin-antitoxin system CcdA family
                     antitoxin"
     gene            1901..2833
                     /locus_tag="HV033_RS24450"
                     /old_locus_tag="HV033_24460"
     CDS             1901..2833
                     /locus_tag="HV033_RS24450"
                     /old_locus_tag="HV033_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_181241460.1"
                     /translation="MSNSALELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRIGAAIDWSDRNAPAPTLTITNPENGHAHLLYALETSIRTAPDGKMK
                     PLRYAAAVENALRKKLGADTGYSGLICKNPNHSHWKIAVWQPELYTLDWLADSLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERASAYNLQFPAPLD
                     ENEVSGIAKSIAKWTKSKFSRDSFESYVAKTHTSEIQRKRGALGGVIGGKVSRGGGRPI
                     GSNDINSLSFEKPWVKENISRSTWYRRMK"
                     /name="replication initiation protein"
     gene            2830..4986
                     /locus_tag="HV033_RS24455"
                     /old_locus_tag="HV033_24465"
     CDS             2830..4986
                     /locus_tag="HV033_RS24455"
                     /old_locus_tag="HV033_24465"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_181241462.1"
                     /translation="MNADLFFDMSESLKLYRRAEIKPKANNNKNPIDDLYVDPLNNNAI
                     LKSILSDNTVFLLGRKGTGKSTVFSKAESVLKGSKDLISVYVDVKSLYDVVSVNDYSDS
                     SQIDKEVSFDVYKMHQARKEILNQILTKLILTFKEKLNSLSIIDSITGKKKKVAQCIEK
                     LDEVRSTIGDQSIENMVLPTLKKITTRITNNQQSENSSTEALSGSMAAKGALSLGVNGN
                     SASFESSANGSVNLSDFQKVLNGTDLYNEYSDTFVRSYPFQKIILSIQEILDDLGMSKL
                     FIFLDDFSEIELIDQRLFVDVILSTLNNSSNEYIKLKIAAYPGRVYYGKLDPAKCTISH
                     LDFYDLYESNETQEMERKACLYTKELIERRFNYYDLNVDDYFDVSTAVNMDSYYYHLFR
                     ASFNAPRIIGNIFHILYKDRISQGSKINASSIDLAARKYYESITSTHFDLLSRFALEPF
                     DNKIDRHNQKELMNFIIDLAKDNRTKIKKGIVGGSFFDGLKVIPTSHFHIKEELSSIFA
                     SLEANYLISRYKKIRNKETETVYVYSMAYGLCVYEKIDWGYPEGREYRAYFQQRCFDYS
                     SRIHQFLSSKSCFKCTNCQHVMRDDEKNNSILEMFDYLCPNCKKSKMVKQNIVSAELDS
                     LMGELNDSLLLEPIELSILGVLNQNGGPLSASEIAGTLDTNRQLIGKRTAKLQEQGLVE
                     KDKESVFNKTMNALTDRAKQLYFS"
                     /name="helix-turn-helix domain-containing protein"
     gene            5226..5552
                     /locus_tag="HV033_RS24460"
                     /old_locus_tag="HV033_24470"
     CDS             5226..5552
                     /locus_tag="HV033_RS24460"
                     /old_locus_tag="HV033_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_181241452.1"
                     /translation="MEKRTKEIKIRMTETEHQRLLERCDRIHLAEWLRQLGLGEQTSRK
                     RPVPEVAPELLRQVSGMGNNLNQIARRLNQSDSLTPSERVSLLSILNSLDRQLGELLEQ
                     HRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5761..>5946
                     /locus_tag="HV033_RS24740"
                     /pseudo=""
     CDS             5761..>5946
                     /locus_tag="HV033_RS24740"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          5543..6012
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.446"
                     /coverage="98.0"
                     /evalue="3.42e-87"
ORIGIN
        1 cgcaggccag cgacttaccc cgtgacaaac agcaagccgc agagaaaatc accgccggac
       61 taatgaacct gatgcagcag ggcgagatcc gcagtcgtca ggatgtggtt aagcagctgg
      121 aacgttacgg cctgagtgtg gcgcgggaaa ccaaaaacag catcagcatt gccgatccgg
      181 acggcggaaa aaatatccgg ctgaaaggaa tgatttatga gcgagatttt aaatttggcg
      241 aagggcttcg aggagaaatc gaagcagcag gcgcaggata ccgagcagag cgtgaagcgc
      301 gagttcgcga ggctggaaac gtctatcagc gaggacttga aatcaagctc gcagagcatc
      361 agcaacgcta tccgcgaaca gaacgaccaa ctgacgggca tagtcagata ctccctcctg
      421 agcgcactgg gctggatatt cctgttcgcc gcgctgctga tcatgatact gggcgggata
      481 atctggtatc agggaacggt catcacttcc cgcctgaacg aaatggacag ctacagccag
      541 caactggaga gcctgaaagc ccagagcggc gcaggggtag cgatttacaa ggacgacaag
      601 cagaagaaca cctatctggt ggtactgccg aagaaagcgc atggcatcga gacatacaca
      661 tcgacagccg gaaacaccgt gatgaagtac accgcgaaat aaccccgaca gagctcacag
      721 aaacgccaca gcacgttttt atcactgagg acaggcaaaa ccatgacaga gatggaaagg
      781 cagcttctga gcgccttaga aagcttacaa acaagttacg aacagcagca gcaggcgtgg
      841 caggacagtt acagcaactt gcaacgcatg ttcgagacta cctcgcaggc actggagcgc
      901 aacgacaggg tatgtctgaa cttgagcaat caggtgaacg ccttgcacga gcaggtcgaa
      961 cgcttgagca gcgatctgag ccgattaaca cgctcgtaaa gcagcaggaa cggcagatac
     1021 ggcagaatac acgttcaacc gtatctgatc gcgggatgac aaggtaaaat cagaacccat
     1081 ccagcagaaa atccagagcg ttcttgatga cctcgcggtg ttcccgaacg cttaccacct
     1141 catcccccag ttgtgacagc cttacgcttg ccatttcatg agtcatgacc agataatcct
     1201 catcctccac attcagcact ggattaagac gacgagcagg ccgtccactg aactcattca
     1261 gcgaatacag gggaatcacc agtcgcgttt tgagttcgcc gataatatcg ctctgcacgt
     1321 ccagcaggta aggataagcc cgactattgc cattattgcg gtaaaccgtg aactgcataa
     1381 tcagaacgtc ctgtaatcgt cagagagtaa gccgtgttcg tccgtaatac ggttcaattc
     1441 ctgaattgcc ttacgattgg tacgcttcca gcgctcagat tcagactcgc gtatctcgtg
     1501 tctcagggca gtggccaatg tagcagacag attgatcccc gcttcgcgtg cctgacgcaa
     1561 aagggctggc tcgagtgtca cactgacggt ttttttatgg cgcatgactg atgttgtcat
     1621 gatgtactcc tgtcttttac acgtgtatat tgcacgtgca ttcagtgtac gcgattctca
     1681 ccagaaaggt aatggcctgt tgcttcgggc aataactgac agtggcaggt gacaaagtac
     1741 ggtggggtgt gtaactttaa gcagcagaaa aaacaaagcc ccggaaatca tgctcaatct
     1801 tggcggagcg acatgataac cagggccagg tacgaaacct aagaaggata ttagcacatg
     1861 aaccgtgcca gacaacagcc cgccataagc gcagggcaca ttgagtaact cagcactcga
     1921 acttttcaac gaccgcttac cgcacaaacc gtacttctcg gatgatttgc actttggtgt
     1981 ccgcatcgca gggaaagaac gcgccattct ggcgaaatac atccagttca atcagccaca
     2041 cgccatgttc tggctgggat ttgacgttga tcgcatcggc gcagccatcg actggagtga
     2101 ccgcaacgct cccgcgccca cgctgaccat caccaaccca gaaaacggac acgcgcacct
     2161 gctttacgcg ctggaaacct cgattcgtac cgcgccagac ggaaaaatga agccgttacg
     2221 ctacgcagct gctgtagaga acgcgctacg taaaaaattg ggcgctgaca cggggtattc
     2281 ggggttaatt tgcaagaatc cgaaccacag tcactggaaa attgcagtat ggcagcctga
     2341 actctacacg ctggactggt tagccgactc actcgacctg aacgccgcga atgataaaga
     2401 aatcgttgcc gattacggct taggccgtaa ctgcaccttg ttcgataaaa cccgtaagtg
     2461 ggcatataga gctatccgtc agggttggcc tgagtatgaa cagtggctgc aagcttgtta
     2521 tgaacgtgcc agtgcttaca atctgcaatt ccctgcacct ctggatgaaa acgaggtgag
     2581 tggcatagct aaaagtatag ctaagtggac caagtcaaag tttagtcgag actcatttga
     2641 gtcttatgtc gcaaaaactc atacatccga aattcagagg aaaagggggg cgttaggagg
     2701 tgtgattggg gggaaagtta gtagaggcgg gggcaggcca ataggctcaa atgacataaa
     2761 tagtctttca tttgaaaaac cctgggttaa agaaaatata agcagaagta cttggtacag
     2821 gagaatgaag tgaacgctga tttgtttttt gatatgtctg agtctttaaa attatacaga
     2881 agggctgaaa taaagcccaa ggcaaataat aataaaaacc caatagatga cctttatgtt
     2941 gatcctctca ataacaatgc aatattaaaa tctatactat ctgacaacac tgtttttcta
     3001 cttggaagaa aaggtaccgg gaaaagtacg gttttttcaa aggctgaaag tgtattaaaa
     3061 ggtagtaagg atttaattag cgtatatgtt gatgtcaagt cattatacga tgttgtttca
     3121 gttaacgatt attctgatag ttcgcaaatc gataaagagg tatctttcga tgtatataag
     3181 atgcatcaag caagaaagga aatccttaat cagatactaa ctaagctgat attaactttc
     3241 aaagaaaaat tgaatagctt atctataata gatagcatta cgggtaaaaa gaagaaagtt
     3301 gctcaatgca tagaaaagct tgatgaggta agaagtacta ttggtgatca gtcaatagag
     3361 aatatggttt taccaacttt aaaaaaaata acaactagaa ttacaaataa ccagcagtct
     3421 gagaactcct caacagaggc attgtctggc tcaatggctg cgaaaggtgc gttgtcgcta
     3481 ggtgtgaatg gtaatagtgc atcttttgaa tctagtgcta atgggtcagt taacctgagt
     3541 gactttcaaa aagtactcaa tggtactgat ctttataatg aatactctga tacttttgta
     3601 agaagctacc cttttcagaa aattatatta agcatacagg aaatacttga cgacttaggg
     3661 atgtctaaat tatttatatt tctagatgat ttttcagaaa ttgaattgat agaccaacga
     3721 ttgtttgtag atgtaatttt atccacatta aataactcaa gcaatgagta tataaaactt
     3781 aaaatagctg cttatcctgg cagggtatat tatggcaagc tggatccagc aaagtgtacg
     3841 atatcacatc tagattttta tgatctttac gaatcaaatg aaactcaaga aatggaaagg
     3901 aaagcctgcc tatatactaa ggagttaatt gaacgtaggt ttaattatta tgatttaaat
     3961 gttgatgatt actttgatgt atctacagca gtgaatatgg atagttacta ttaccatttg
     4021 ttccgagctt cgttcaatgc tccccgaatt attggtaata tttttcatat tttgtacaaa
     4081 gatagaatat cacaaggtag caaaataaat gcatcatcta ttgatttggc tgcaagaaag
     4141 tattacgaaa gtataacatc aactcatttt gatctattgt ctagatttgc cttggaacct
     4201 ttcgacaaca aaatagacag acataatcag aaagagctaa tgaattttat aattgattta
     4261 gccaaggata atagaactaa aataaagaaa ggtattgtag gggggagttt tttcgatggt
     4321 cttaaggtta tccctactag ccatttccac ataaaggaag agttatcttc tatcttcgca
     4381 tcgctagaag ctaactatct gatatctagg tataaaaaaa tccgtaacaa ggaaacagaa
     4441 actgtttatg tatattccat ggcttacgga ttatgtgttt atgaaaaaat tgattgggga
     4501 tatcctgaag gacgtgaata ccgagcttat tttcagcaaa gatgttttga ctattcatca
     4561 aggattcatc aattcctatc gagtaaaagt tgcttcaaat gcactaattg ccagcacgtg
     4621 atgcgtgatg atgagaagaa caattcgata ttagaaatgt tcgattatct ttgcccgaat
     4681 tgtaaaaaat cgaaaatggt gaagcaaaat atagtttcag cagagttaga ttcgttgatg
     4741 ggggaactta atgatagcct acttctagaa cctattgaat tatcaatact tggtgttcta
     4801 aaccagaatg gtggcccatt atccgcatca gaaattgccg gtacactgga tacaaaccga
     4861 cagctcatag gaaaaagaac agcgaaactt caggaacaag gccttgttga gaaggataaa
     4921 gagtccgttt ttaacaaaac tatgaacgcc ctaacagata gagccaagca actatatttt
     4981 tcataatctc acattgagac aaaaaacaaa gcctatatca gataacagcc ctttcaaagg
     5041 gctgtttctt tttgcatatg ttccccgata tgacatactt aaatttatct taagccagta
     5101 cacacggagt cccctctaac gaggtgccac cgtgactggt tcagggggct cgccgccccc
     5161 cgaaaccccc ggcatccact gcgaaaaatt cccacagtgg tgcgaatttt ttctcagcgg
     5221 agcctatgga aaagcgaacg aaagagatca aaatccgtat gaccgagact gagcatcagc
     5281 gtctgcttga acggtgcgac cgtattcatc ttgcagagtg gttgcgtcag cttggtctgg
     5341 gagaacagac atcacgtaaa cgacctgttc ctgaggttgc gcctgaattg ctccggcaag
     5401 tgagtggcat gggcaataac ctgaaccaga tcgctcgccg attgaatcag tccgattccc
     5461 tgacgccttc agagcgtgtt tcactgcttt ccatcctgaa cagcctcgac cgtcagctag
     5521 gggaattact ggagcagcac cgtgatcgtt aaaattcatt cccgtggtgc aggttctggc
     5581 agtggtccgg ttgattacct gctgggaaaa gaccgtcagc gagagcaggc taccgtatta
     5641 cggggtgatc ctgagcgtgt atgcgaactg atagacagct gcgactttgc ccgttcgtat
     5701 acctccggtg tgctgtcttt tcaggagccg gatatcgccg actctgagaa atcccgcctg
     5761 atggacgagt gggaacagac cctcatgacg ggattagacc gtgaccagta caactgtctc
     5821 tgggtagagc accgcgataa gggacggctt gagctgaatt ttgttatccc gaacatcgaa
     5881 ttgcagagcg gaaaacggct gcaaccctac tttgaccgcg ccgaccgtcc cagagtgaat
     5941 gcgtggcaga cgctgactaa tgaccgactc ggattacgtg acccgaatga cccccttaac
     6001 cgccgtccga tga
//
