LOCUS       NZ_CP058190             2495 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00016 plasmid pRHBSTW-00016_4,
            complete sequence.
ACCESSION   NZ_CP058190
VERSION     NZ_CP058190.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148453
            Assembly: GCF_013635255.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88.6188692108241x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013635255.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:48:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,898
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,673
            CDSs (with protein)               :: 4,673
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 31 of 107
            Pseudo Genes (incomplete)         :: 78 of 107
            Pseudo Genes (internal stop)      :: 22 of 107
            Pseudo Genes (multiple problems)  :: 21 of 107
            Pseudo Genes (short protein)      :: 1 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058190.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00016"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00016_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            507..698
                     /locus_tag="HV036_RS24360"
                     /old_locus_tag="HV036_24370"
     CDS             507..698
                     /locus_tag="HV036_RS24360"
                     /old_locus_tag="HV036_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021243041.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQILSETEPAV
                     RTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(989..1174)
                     /locus_tag="HV036_RS24365"
                     /old_locus_tag="HV036_24375"
     CDS             complement(989..1174)
                     /locus_tag="HV036_RS24365"
                     /old_locus_tag="HV036_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1540..1767)
                     /locus_tag="HV036_RS24370"
                     /old_locus_tag="HV036_24380"
     CDS             complement(1540..1767)
                     /locus_tag="HV036_RS24370"
                     /old_locus_tag="HV036_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          855..942
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2..228)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.52"
                     /coverage="97.0"
                     /evalue="3.19e-75"
     Typing          complement(2003..2485)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.96"
                     /coverage="95.0"
                     /evalue="2.29e-126"
ORIGIN
        1 ggtttcgcca ccactgattt gagcgtcaga ttctgtgatg cttgtcaggg gggcggagcc
       61 tatggaaaaa cggctttggc gcggccttat gctttcttcg ttaagtatct tcctggcatc
      121 ccccaggaaa tttctgatcc atccgtaagc ccgtcccgct cgccgcagcc gaacgaccga
      181 gcggagcgag tcagtgagcg aggaagcgga atatattctg tatcacattt tctcctgacg
      241 cgttttcttt cactttctgc gcctgtctta tgtggcatta atgctatgtg ttactgccat
      301 gctacatctt aagccagtat acactccgct agtgctccgt gactggtccg gcgctgcgcc
      361 cggaacccgc ctgtaccggt tcagcagccg ttccggcctg actgcaattt tttttttttc
      421 atccctgccc gctaccctgt aaacctttct tctgcgttgc cgttaacagt aggtaagcca
      481 tgtttggttt ttcttccgga gccctgatga ctcttagtga tgatgagcga cacctgcttg
      541 tgtccgttgt ttcggtctgg ctacggcgtg ccggagggga tgccggagcc atgatgctgg
      601 atgcataccg gcagattttg tctgaaaccg aaccggccgt aaggactgtc atgctcgaat
      661 ttcttgaatc cgttcgcatt cattacattt cgtcctgagc attttctgta tttctgtcca
      721 tacttaaacg aggcggccgt cacggacggt ggtcgtcgtt ccatgatgct gttatggacg
      781 agtgagagta atctaatcta acttgattga gttccgggcg tgccgcccgg ttttttttgc
      841 tgtttcccgg tgtccgttcc gggacaggat gtgcatttgt aataccgcgc acgcgccggc
      901 atcacaaata ccctggtcag ggctgcgccc cgacaccccg ccatcgcctc acggccgcgc
      961 actgaattgc gcacccgttc accgattttc acgcattttc ctcgcctatc tgtgcgttca
     1021 gcatggcgta aagcgcttcg gcctgatcgt gcaggtgctc acagcagccg gcagcctcat
     1081 ccagatccat ctgctcgagc cgctcaagga ggagaagtgt ctgtgcgcgg ataaatttgg
     1141 ccatattgag ggcggcggat tcctgtctgg tcactggtga ttttccccgt ctggtgatga
     1201 tttttgcgtg aaaagagcat ggcgtcattc tggtgcatag tcatgcatgc cgttaaaatt
     1261 taacaggcgc gtttctgacg ggttccgggg tggtttgttg tggtttttgt catggtttcg
     1321 tcaggaacgc gctgaacgcg tctcaggcgg tgcgggcaac ggatgttatg gtaaattctt
     1381 gggggcttga ttataaaata ccgcacttcc tcttatgtct tgtgcttttt agacaaccct
     1441 gaaagctttt agctttggtg atgcaatttg ctgggatttt ggcggagaac cacaggtaaa
     1501 gaaaaaggcc acattagcgg cctttttcgg agaagatgct cagtgcggaa tttcggagac
     1561 ttctttaacg tcggttttat tgatctgctg tttgacacca ttcgcgtctg tatagccaag
     1621 caggcctgag tctgattctt taggtttgcc gtcgctgaca atagtgcgac cgtcattggt
     1681 gtgtatggca tagcttgtgc gcgtgcagcc ggtgagggct gaaagcgcaa ctgccgctgc
     1741 gaacactgaa acaaaaatct ttttcaaagc cagctccttt tatccatatc agttttggtc
     1801 actaaccatc taactatagg tcatttttta tgtgaaaaga ggcttgatgg tgggctgtat
     1861 atcgagttag gcgcgaggtg ctatggtcaa agtgtggtgt caggcgggga ggaggcttat
     1921 atgagatgta atccggccga tacatatacc aagatgattt aagtattcct ctttgtgagg
     1981 ttggatgtct aatttataaa ggatcttctt gagatccttt ttttccgcgc gtaatctctt
     2041 gccctgtaaa cgaaaaaacc accctggcag gtggtttttc gaaggttagg taatcctggc
     2101 agatccccta accgtggtaa cagtcttgtg cgagacatgt caccaaattt gtcctttcag
     2161 tgtagcctca ctaaggccgc cacttcaaga actcttgaga catctctcgc acatcctgtt
     2221 tgccaatggc cgttgccaat ggcgattagt cgtgtctttc cgggttggac tcaagttgat
     2281 agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccatcc
     2341 tggagcgaac tgccttcccg gaactgagtg tcaggcgtgg aatgagaaac cgcggccata
     2401 acagcggagt gacaccggta aaccgaaagg caggaatgcg ggggagcacg agggagccac
     2461 cagggggaaa cgcctggtat ctttaagccg catcg
//
