LOCUS       NZ_CP058186             3294 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00039 plasmid pRHBSTW-00039_7,
            complete sequence.
ACCESSION   NZ_CP058186
VERSION     NZ_CP058186.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148459
            Assembly: GCF_013635015.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00039
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 74.5883535266037x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013635015.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 05:29:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,253
            CDSs (total)                      :: 6,137
            Genes (coding)                    :: 5,991
            CDSs (with protein)               :: 5,991
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 53 of 146
            Pseudo Genes (incomplete)         :: 102 of 146
            Pseudo Genes (internal stop)      :: 17 of 146
            Pseudo Genes (multiple problems)  :: 24 of 146
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058186.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00039"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00039_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(1140..1562)
                     /locus_tag="HV042_RS30985"
                     /old_locus_tag="HV042_30990"
     CDS             complement(1140..1562)
                     /locus_tag="HV042_RS30985"
                     /old_locus_tag="HV042_30990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_080922785.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVID
                     TSELIRIFGSLYGGEDTVKHDVNGDAGATAVNILGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1562..1753)
                     /locus_tag="HV042_RS30990"
                     /old_locus_tag="HV042_30995"
     CDS             complement(1562..1753)
                     /locus_tag="HV042_RS30990"
                     /old_locus_tag="HV042_30995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_080922787.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNEFDLDIEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2476..2955)
                     /locus_tag="HV042_RS30995"
                     /old_locus_tag="HV042_31000"
     CDS             complement(2476..2955)
                     /locus_tag="HV042_RS30995"
                     /old_locus_tag="HV042_31000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]; GO:0008080 - N-acetyltransferase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_080922786.1"
                     /translation="MANFSCGVTHLDEFLKTDLHKMQGRSLLKGYVLLSDDEIPEIMGY
                     YTLSGASFERGHLSNRLRKKIPYSNMPAVLLGRLAIDQRIQRKGFGELLIVDAIHKVRA
                     TAGQIGIYAMFVEAHPGASSFYTRMGFIPSSMADDNKTFFYPANQFDDLIFGDKC"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(2997..3281)
                     /locus_tag="HV042_RS31000"
                     /old_locus_tag="HV042_31005"
     CDS             complement(2997..3281)
                     /locus_tag="HV042_RS31000"
                     /old_locus_tag="HV042_31005"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="WP_044316655.1"
                     /translation="MNAVRKERFEFRLPEAAKRRIEEAAVISKVSVSQFVMESATFRAE
                     TVINEHRRLIVEEDVWEQVMAALERPPAPTQSMCKAFERYNSEESWICD"
                     /name="DUF1778 domain-containing protein"
     Typing          1802..2283
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.858"
                     /coverage="92.0"
                     /evalue="1.31e-148"
ORIGIN
        1 ctaatgctca cagtggttta taccacatga cgatatgcac ggtacggtaa tttaccgtac
       61 aggtaatttg aggtgatttt gatctgagaa actatgccag tcaagctaat gaagggtgag
      121 ggtagtattt tttgagaaaa aaacttaaac ttcggaaaaa taaccttaaa aaacaacaac
      181 ttaaccatgg caatgacact taggaggcgt tggtggttct taagaaccac caacgcctcc
      241 taagtgtcat tgccagttta ttttatcatt tttttgcatg taaatctacc gctattttga
      301 acgaatgtat gggccaaatc acacagcatt caggggatgt gcattacggt attttgcgcc
      361 ccataaatcc acccacgcac tccaaaacaa gtcactgatc cagatttacc ataacgtctc
      421 attacgcgca ccgctcaaac gctccagaaa cgattctggg gcgtaaccca ttaaatccta
      481 cgtcttaccc cctgaaaacg cctcaaaatc gcgctgatgg gttttacggg tatgaattgt
      541 tatgcaccag actggctgga ggcagatttc atcctgaatt cacgccagac aactcaaaac
      601 ccgcaccaaa cccgactgaa aacggtgaac ggggacgcaa tttattgcgt ggccgtgaag
      661 cgttgccggc gcagccggaa aaccggtttt cagcacgttc aggaatcgag ttcgtcgacg
      721 cccggcaatc acgctatggc aagtcgccgg acgactcacc acacgattca ccggcgactc
      781 ttcactagag tcgtacctag agtcgccgag tctaaaaaaa accaaccggg gagtcgggtc
      841 tagagtcggg tcgttattaa ctcattgttt ttatagtcct gagtcgttac cgactctatg
      901 gcgactcgcc ggggactcta tgacgactcg tcatagagtc ggatctatgt aaatgaaatt
      961 taaaaaacga aatatcagga aaataaacta ctgatattaa atacaaatct gcaaaaattt
     1021 aattttgcgt agtgtgtggg tatttttagt ggtgaggagc gttcagagat ctgacgattt
     1081 ttgggaaggt tcaggaactc cacgggatgg ccggaaagga aaactgaagc atgacggggt
     1141 caggattttt tccagaattt ccaccacgga gaatgaactt gtggagaggg ttcgtgcttg
     1201 tgttccagaa gctgcatagc ctggcgaaga gaatcgatat gttcatcacg agctttaaca
     1261 gcccctttaa gaccatcgtt ttcagcttca agcaaagcaa ttttatgctg taaatcatgt
     1321 aaaccaagga tgttaaccgc tgtagcgcct gcgtcaccgt ttacatcatg ttttacagtg
     1381 tcttccccac catacagcga gccaaaaatc ctaataagct ctgatgtatc aataacttta
     1441 gtgtttttcc catgctttac aacagaggtg taacttagtt tcccactgtt aatcatcttg
     1501 taaattgtgg ggcgactaac cttaacaagc cgtgccgctt cactaataga cactctggcc
     1561 atcagttacc atcctgtaat tcatccagtc tcgaggacag tgtacggaaa agatgctccg
     1621 catcatcgtg gagtttctca caaaggtcgg cctcgatatc gagatcaaac tcgttaagct
     1681 tctccagcag aagcagagac tgattctgga tgaatttagc catattcagc gctgttttct
     1741 gttgtttagt cataaagggc ctcagagcgt cagtgctcag tgtcaaacca gtctcttttc
     1801 cgcttcctcg ctcactcagt cgctatgctc ctgccgttcg actgcggcga gcggtcgcaa
     1861 ctcacgtata agggcatgag acgagagcgc ggatagccag agaggacaag gagaaggccg
     1921 cggcgttgcc gtttttccat aggctccgcc cccctgacaa gcattacaga aacaaacgct
     1981 caaatcagtg gtggcgaaac ccgacaggac tataaagata cctatcattt ctgataggtg
     2041 gctccctcgt gcgctctcct gttcctgcca ttcggtttac ccgtgtcatt ccgctgttat
     2101 ggccgcgttt ttctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
     2161 ctggactgta tgcacgaacc ccccgttcag tccgactact gcgccttatc cggtaactat
     2221 cgtcttgagt ccaacccgaa taggcacgac aaagcgccac tggcagcagc cactggtaac
     2281 tgattagagg agttagtctt gaagttatgc gccggttaag gctaaactga aaggactgat
     2341 ttggtgacta gctcctcttt agccagttac cttggtttag tgaacttgcc ggttcaccaa
     2401 ccttaaaaaa gccaccttgc cgggtggttt tttttttact ttcacatata tctcatagat
     2461 atatgtgaaa gtactctagc atttatcgcc aaagataaga tcatcaaact gattagctgg
     2521 gtagaaaaat gttttattat catcagccat tgatgatgga atgaatccca ttcttgtgta
     2581 aaaagatgaa gctccaggat gcgcctcaac aaacatggca tagataccaa tctgtccagc
     2641 agtagccctc actttatgga tagcatcgac aattagtaac tcaccaaaac cttttcgctg
     2701 aattcgttga tcgatagcaa gacgacctaa cagcactgcc ggcatgttcg agtaaggaat
     2761 tttcttcctc aggcggttac tcaaatgccc tcgttcgaac gatgcaccgc tcagcgtgta
     2821 gtaccccata atttctggaa tttcatcatc agaaagcaga acgtagccct ttaataggct
     2881 acgcccctgc attttgtgga gatcggtttt caaaaactca tctaaatgtg tgactccaca
     2941 gctaaagttc gccatgctgt actcagcaca caagtcaaaa ggttgaaaac cagggattaa
     3001 tcgcaaatcc aactctcctc agagttataa cgctcaaacg ctttacacat actttgtgtt
     3061 ggtgctggag ggcgttcaag ggcagccatg acttgctccc aaacatcctc ttcaacgatc
     3121 aagcggcgat gttcattaat aactgtttca gctctaaaag tcgcagactc cattacaaat
     3181 tgagatacgg acactttaga aataacagca gcttcttcaa tacggcgctt cgcagcttcg
     3241 ggcagacgaa actcgaaccg ctcctttctt actgcgttca taacaactcc tgag
//
