LOCUS       NZ_CP058185             3514 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00039 plasmid pRHBSTW-00039_6,
            complete sequence.
ACCESSION   NZ_CP058185
VERSION     NZ_CP058185.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148459
            Assembly: GCF_013635015.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00039
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 74.5883535266037x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013635015.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 05:29:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,253
            CDSs (total)                      :: 6,137
            Genes (coding)                    :: 5,991
            CDSs (with protein)               :: 5,991
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 53 of 146
            Pseudo Genes (incomplete)         :: 102 of 146
            Pseudo Genes (internal stop)      :: 17 of 146
            Pseudo Genes (multiple problems)  :: 24 of 146
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058185.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00039"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00039_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(3469..3514,1..170))
                     /locus_tag="HV042_RS30960"
                     /old_locus_tag="HV042_30965"
     CDS             complement(join(3469..3514,1..170))
                     /locus_tag="HV042_RS30960"
                     /old_locus_tag="HV042_30965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(177..674)
                     /locus_tag="HV042_RS30965"
                     /old_locus_tag="HV042_30970"
     CDS             complement(177..674)
                     /locus_tag="HV042_RS30965"
                     /old_locus_tag="HV042_30970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<715..1413)
                     /locus_tag="HV042_RS31640"
                     /old_locus_tag="HV042_30975"
                     /pseudo=""
     CDS             complement(<715..1413)
                     /locus_tag="HV042_RS31640"
                     /old_locus_tag="HV042_30975"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1352..1693)
                     /locus_tag="HV042_RS30975"
                     /old_locus_tag="HV042_30980"
     CDS             complement(1352..1693)
                     /locus_tag="HV042_RS30975"
                     /old_locus_tag="HV042_30980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2864..3055
                     /locus_tag="HV042_RS30980"
                     /old_locus_tag="HV042_30985"
     CDS             2864..3055
                     /locus_tag="HV042_RS30980"
                     /old_locus_tag="HV042_30985"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040118553.1"
                     /translation="MLKKNEIILIKSCGNSKTKIFLRGVDEGLMIDASFEDIKFAINNN
                     LHDLLGIQAETTKLKLAK"
                     /name="hypothetical protein"
     Typing          complement(1779..1951)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.655"
                     /coverage="100.0"
                     /evalue="9.54e-56"
     Typing          1876..2422
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(2..1362)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1359..1672)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="9.860000000000001e-59"
ORIGIN
        1 tgcctgctca agcccgacac gcgctcgctc agcgccgcgt tctcccgttg cataagacca
       61 gacatcttcc gccattcctc gaaggcgctc tcccactcgt ccagccttct cgagtagtcc
      121 tgctgtagct gctctaacgc gctcagcaat tgtttttcca gttccgtcat tctgctctac
      181 tcccgtacca gcttcaccgc gttccgcgtc ttgttgtcca ccgtccggcc cgtttcggct
      241 ttcatcccct ccggcagcac cagaaaccgt ccgttgcggt tctccagata ggtcacgccc
      301 cacgtctgca tgtgcagttt cgacagcgta tctctctgct gcctgatttc cgccaggttc
      361 gccgctatcc ggttcccctg gtaccacagc accccgctca ggctggcgaa cagcagccct
      421 gacaccatgg tgatggtcag ccacgtccgc ccgaccattc gcagcacgct cagccggttc
      481 gactgcatcg ccgcgttcat cccctgttcg tgggcgctga tggcgtcgtt gattctcttc
      541 gcgcttgcgt tcagttccga tttgacaacg ttctcgtgct tgctgaatgc ggctttcagc
      601 ctctcttcgg taagctgctg ctgtgctttc gatttctcct cgaagtccct cgaggccgtt
      661 aaaagactgc tcataaatgg ctcccctcag tcggatattt cgccccccgt ccgggtcggc
      721 aatgctgata ctgttttttg tggttctgac cacctcaaaa ccggccgctg tgagcgcccc
      781 tgtgacgtca tcacgcgttt tcagctctcc tgatgaggct aacatcagca aaccccgtgt
      841 aatcgtctct gcggcttcct gcttcgtttc tggcagtcct gaaggcgtga ccagcgcccg
      901 ccggttctcc gggtcgttcg gatcatgtag ccccagcctg ccgttgacca ctgtctgcca
      961 cgcatcgatg cgcggccggt cggcacgatc gtaatacggc tggaggcgtt tccccgtcag
     1021 taattcggta ttggggatca gaaaattcag ctcaagacga cccttgtctg cgtgttccac
     1081 ccacagaatg ctgtactggt ctttatcgag tccgggcatc agaacccgct caaagctggc
     1141 catcagtttt tcacgctgac ccggcggcag gtccgcctcc gcgaatgaca gcaccccgga
     1201 ggtatatttt ttcacatacg gcgacgcatc aatcagctcc cggacttctt ccggcttccc
     1261 ctgcaggacg ctggcacctt cacgctgccg gtctttaccc agaagataat ccacaggacc
     1321 ggcgcccccg ccgcgacccc ggggatggaa tttaacaatc atcgtccggc cctttttcca
     1381 gtacggcatg ccgcaaccgt tccagaccgg catcgatcgc catcagcgct gccacgatct
     1441 gcacccggtc atgaccggca ccaccggcgt tcactttccg ggcgatctgg ttcaggttat
     1501 tgcccatgcc ggcaagctga cgcagcagcg cgggcgaaat cgagggaagc ctgccggcgc
     1561 gtgtcggttt ctcatcaagg caggtctggc gcatccacgc cgccagttgt tttccgtcgc
     1621 accgctcaag caaccgccgg tgttcgtctt ccgtcaccca catcgtgagc atcttgttgc
     1681 gtttatctgc cagaatcgcc tcctgataac gccgtgacgg acgggaaagc ctttcccggt
     1741 cggcgcgggg ttggacaagc cgcaggcgcg tcagtggggt ttcggggcgc agccctgaac
     1801 cagtcacgta gcgctagcgg agtgtatact ggcttagtat gttggcatga gtgcgataat
     1861 caggaaagcg ccttatgcgg cagaagaaaa atgctgcagc agagcgtaag cagaatatgc
     1921 gatacagaag atattccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1981 ggcgagcggt gccggcttac gggcggggcg gaaatttcca ggaagatacc aggaagaaac
     2041 ttaacaggga agcgatgagg ccgcggcgaa gccgtttttc cataggctcc gcccccctga
     2101 caagcatcac aaaatctgac gctcaaatca gtggcggcga aacccgacag gactataaag
     2161 atcccaggcg tttccccctg gtagctccct cgtgcgctct cctgttcctg cctttcggtt
     2221 taccggtgtc attccgctgt tatggccgcg tttatctcat tccacgcctg acactcagtt
     2281 ccgggaaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgact
     2341 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacac gccaaagcgc
     2401 cactggcagc agccactggt aacaggatta gcagagcgag ttaatgtagg tgatgctaca
     2461 gagttcttga agtggtggcc taactacggc tacactggaa ggacagtatt tggtatctgc
     2521 gctctgctga agccagttac ctggttaagc agttccccaa ctgacttaac cttcgaaaaa
     2581 ccgccctgca aggcggtttt ttcgtttaca gagcaagaga ttacgacgag gctgaatgaa
     2641 tctcaagatt tcatttagcc tgccacccga tcattcaaag ttttggcgta gttctgaccc
     2701 gctccccgtg atttgttaca tacccaccac agggcattag tttttttatt tttttgatga
     2761 tatgtacaaa atcagtggtt aggaataatc ttagcagcct taacaaacac acacaaaaac
     2821 aacgataatg attcatctga cgacaacaaa caatgacaat gtcatgttaa aaaaaaatga
     2881 aattatctta ataaaatcat gtggtaactc aaaaacaaaa atttttttac gaggtgtgga
     2941 tgagggactc atgattgatg caagttttga agatataaaa tttgctatca acaataacct
     3001 ccacgatctt ctcggaatcc aagccgaaac cacaaaacta aaactagcaa agtgatacag
     3061 cccctgaagc atgaaggatc gaaataaagc caccgattta ccataacgtc ttcttacccg
     3121 caccgcgccg gaggcactca gggcgtttct gatggctgac gggcaaaagg tggtggcaag
     3181 tacaccgaaa accaccagaa acgcacctga tttattttac gcatatgcat cacttattca
     3241 catcactgca tagccggtga tttcggttaa aaaagactct ctgtgtgctg aaatcgcaga
     3301 aaatggccat gatccccgtc acgctcaggc cacctgagac gggcgtttat cagtctccat
     3361 actccctgtc accatgagat aatgtgcgtt gccgttccgg taccagatct gctgtcttct
     3421 ctggttcctt ttccgttgtt tgcccgtatc gctgttgctc aatctcattc agcctgtcga
     3481 taaccggcgc agctgctcgc tcaaactctg cacc
//
