LOCUS       NZ_CP058179             4096 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00040 plasmid pRHBSTW-00040_5,
            complete sequence.
ACCESSION   NZ_CP058179
VERSION     NZ_CP058179.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148460
            Assembly: GCF_013629675.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00040
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.5072874969504x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013629675.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:51:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,888
            CDSs (total)                      :: 4,770
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 35 of 143
            Pseudo Genes (incomplete)         :: 107 of 143
            Pseudo Genes (internal stop)      :: 19 of 143
            Pseudo Genes (multiple problems)  :: 18 of 143
            Pseudo Genes (short protein)      :: 1 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058179.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00040"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00040_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(4017..4096,1..352))
                     /locus_tag="HV043_RS24195"
                     /old_locus_tag="HV043_24205"
     CDS             complement(join(4017..4096,1..352))
                     /locus_tag="HV043_RS24195"
                     /old_locus_tag="HV043_24205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001131614.1"
                     /translation="MPLTRLRLAQHRADREKISRPSRRYQEAGLADKRSKMLTMWVTED
                     EHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSISPALLRQLAGMGNNLNQIARQVN
                     AGGSSGLDRVQVVAALMAIDAGLERLRHAVLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            883..1041
                     /locus_tag="HV043_RS24650"
     CDS             883..1041
                     /locus_tag="HV043_RS24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032670878.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHEKSLLAAAISNWRYTRDKL
                     RGLEVWA"
                     /name="hypothetical protein"
     gene            complement(1278..1547)
                     /locus_tag="HV043_RS24200"
                     /old_locus_tag="HV043_24210"
     CDS             complement(1278..1547)
                     /locus_tag="HV043_RS24200"
                     /old_locus_tag="HV043_24210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_079839079.1"
                     /translation="MNGQEFRLLLTEHGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYL
                     EDAHKNELLIELNESDLYPGGAIELQHNGGRKIHCEILKVRWSR"
                     /name="hypothetical protein"
     gene            1629..1862
                     /locus_tag="HV043_RS24205"
                     /old_locus_tag="HV043_24215"
     CDS             1629..1862
                     /locus_tag="HV043_RS24205"
                     /old_locus_tag="HV043_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461706.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_001036746.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDDRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1884..2111
                     /locus_tag="HV043_RS24210"
                     /old_locus_tag="HV043_24220"
     CDS             1884..2111
                     /locus_tag="HV043_RS24210"
                     /old_locus_tag="HV043_24220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_000719360.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(2641..2850)
                     /locus_tag="HV043_RS24215"
                     /old_locus_tag="HV043_24225"
     CDS             complement(2641..2850)
                     /locus_tag="HV043_RS24215"
                     /old_locus_tag="HV043_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001632067.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMCGLMQRENA
                     ALSERVTDLSTQVLSLSEQLRRLS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2854..3339)
                     /locus_tag="HV043_RS24220"
                     /old_locus_tag="HV043_24230"
     CDS             complement(2854..3339)
                     /locus_tag="HV043_RS24220"
                     /old_locus_tag="HV043_24230"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012782630.1"
                     /translation="MNSLLTLAKDLEQKSKVQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAIHDHDRMLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLTTCGEERRRCVRVNPDAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<3374..4078)
                     /locus_tag="HV043_RS24655"
                     /old_locus_tag="HV043_24235"
                     /pseudo=""
     CDS             complement(<3374..4078)
                     /locus_tag="HV043_RS24655"
                     /old_locus_tag="HV043_24235"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(353..431)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          507..995
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.747"
                     /coverage="94.0"
                     /evalue="7.82e-151"
     Typing          complement(3..244)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.296"
                     /coverage="99.0"
                     /evalue="1.9e-45"
     Typing          complement(2532..4027)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.998"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgacgacctg cacgcggtcg agaccgctac tgccaccggc gttaacctgg cgggcaatct
       61 ggttgaggtt gttccccatg ccggcaagct gacgaagcag cgccggcgag atcgagggaa
      121 gtttgccggc ccgggcgggc ttctcgtcca ggcacgtctg ccgcatccag gccgcgagct
      181 gtttaccgtc gcagcgttcc agcagacggc ggtgctcatc ttctgttacc cacatcgtga
      241 gcattttgct gcgtttgtct gccaatccgg cctcctgata acgccgggac gggcgggaaa
      301 tcttttcccg gtcggcgcgg tgttgggcaa gccgcaggcg cgtcagtggc atttagcggg
      361 tttcggggcg cagccctgaa ccagtcacgt agcgctagcg gagtgtatac tggcttacta
      421 tggtggcacg acgggaatcg ggatgaaata gtcagtcatg aaaactgtga gatgaggctc
      481 aggtgcgtaa gaggctgatg cctgtcccgg tggatattcc gcttcctcgc tcactgactc
      541 gctacgctcg gtcgttcggc tgcggcgagc ggtacggact cacggataaa gacgtggcgg
      601 ggaaatacgt gaatgcagga ggggaagaaa ggatgcgccg ctgccggttt tccataggct
      661 ccgcccccct gacaagcatc acagaatctg acgctcaaat cagtggtggc gaaacccgat
      721 gcggcttaaa gataccaggc gtttccccct gatggctccc tcgtgctccc ccgcattcct
      781 gcctttcggt ttaccggtgt cattccgctg ttatggccgc ggtttctcat tccacgcctg
      841 acactcagtt ccgcgaaggc agttcgctcc aggattgact gtatgcacga accccccgtt
      901 cagtccgact gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacac
      961 gaaaaatcgc tactggcagc agccattagt aactggaggt atacgagaga caaattaaga
     1021 ggtcttgaag tatgggcctg agagaggcta cactgaaaga acagttttgg tttctgcggt
     1081 ctcgtatacc cagttacctg gttaagcagt tccccaactg acttaacctt cgatcaaacc
     1141 acctccccag gtggtttttt cgttttcaga gcaagagatt acgactgtaa cgacaggatc
     1201 acaagaagat catttactca gagagactaa ctaccactat gtttgcctat caacatactt
     1261 aggaaacatt acttgagtta acgactccac cttactttta gtatctcgca atgaatcttc
     1321 cgaccgccgt tgtgttgaag ttctattgcg ccaccggggt ataaatcact ttcgtttaac
     1381 tcaattaaaa gctcgttctt gtgagcatcc tctaaataaa ggtaataaca cccaccataa
     1441 aggttctgcg tatctggcac ggcaacaaaa gatttctttc cgaacagtaa aacagcctct
     1501 ttttctccat gctctgtcag caataatcga aattcttgtc cattcaatcc tcttttcgca
     1561 tgaaaaaacg acctatactt agatggttag tgaccatatc cactttacct ttaagcaggg
     1621 aacctattat gaataaaaaa acgctgttgg cggccatgat ggtcgcgttg gtgacctttg
     1681 ccgctgctgg atgttcttca aaccaagaaa taaaaaccac agatgatcgc actatcgtta
     1741 cagacggcaa acccaaagtt gacagtgata cgggattagt atcctacaaa gatgcccata
     1801 caggccgaac cgaacagatc aaccgtgaca aaattaccaa catgagtgaa ctagacaact
     1861 gatacggata aaaggagctg gctttgaaaa agatttttgt ttcagtgttc gcagcggcag
     1921 ttgcgctttc agccctaacc ggctgcacgc gcacaagcta tgccatccac accaacgacg
     1981 ggcgtacaat tgtcagcgac ggcaagccta cagaatcaga ctcaggcctg ctgggctata
     2041 aagacgcaaa tggtgtcaaa cagcagatca ataaagccga cgttaaagaa gtctctgaaa
     2101 ttccgcattg aggcatcgct ctctccgaaa agggccgcta atgcggccct tttctttatc
     2161 tgccccaaca acaggcaaaa aaagcccccg ctgggggcca aatacctgca gtggctggat
     2221 agtactgcat tcctgatgaa ggagacgaat aagtatgggc cggatttttg cgtctgcagg
     2281 ctcggtcgcc acaatttttc cgtacccgcc aggcatcatg cttttaccat aacatccctt
     2341 gcccgcacct ctccggacgg gttcagcgcg aatgcggcgt cagtcctgca aaaccgacag
     2401 taaaccaccc cgcaaaccgt gcattctgcg cctgataaat ctcacccgac tgcatagcca
     2461 tgcagccggg tgaatggcac cgggtttcgg cgcagatcct caatgaaagc cccgttttat
     2521 ccggttatca cagcgacgga ccgtcatact cctgctgacg ctgcgatcgt tccagctcca
     2581 gcgctttctg gtgctgcgct gcgcgctcct gctcccggtg tgcctcgatg tcgttcagcc
     2641 ttacgacaac cggcgcagct gctcgcttaa actcagcacc tgcgtgctca agtccgtgac
     2701 gcgctcgctc agcgctgcgt tctcccgttg cataagacca cacatcgtcc gccattccgc
     2761 gaaggcgctc tcccactcgt ccagcctttt cgagtagtcc tgctgtagct gctctaatgc
     2821 gctcagcaac tgtttttcca gttccgtcat gtgctatttc cccgccagta tcatccagct
     2881 cgaatcctct ccgaaccgtc ccgcgtccgg gttcaccctc acgcagcggc gtcgttcctc
     2941 tccgcaggtc gtgagctgca cgccgctgtt cctctcggac agcatggcct gcgtgctctt
     3001 ctgctcccgg atggtcgtgt aattgtcgag tatctgctgc ccctgccacc atagaatgcc
     3061 cgcacttgag gctatcagca gcacggagac caggacgatg gtcagccacg tctggctgac
     3121 catgcgcagc atccctttcg tacgctggct catggctgag gacagcatcc ggtcgtggtc
     3181 gtggatggcg gcgctgattc tcttctcgct ttcgttcagt tccgctctga cagacttctc
     3241 gtgctcgctg aatgcggctt tcagcatctc gccggtagtc tgctgctgca ctttcgattt
     3301 ctgctctaag tccttcgcca gcgttaaaag actgttcata gatggctccc ttcagtcgga
     3361 tgtttcgccc cccgtccggg tcggcaatgc tgatgctgct ttttgtggtg cgaaccacct
     3421 caaaacctgc gctttccagc gcctcagtga cgtcctgacg cgttttcagc tccccggacg
     3481 aggctaaggc cagtaaaccc cgcgtaatcg cctgagcggc ttcctgcttc gtttccggca
     3541 gcgcggacgg cgtgaccagt gcccgccggt tttccggcgc gttcgggtcg tgcagcccca
     3601 gactgccgtt cacgatggtc tgccaggcat caatgcgagg acggtcggcg cggtcatagt
     3661 atggctggag ccgtttgccg gtcagcagct ccgtgttcgg gatcaggaag ttcagctcca
     3721 gccgcccctt gtcccggtgc tcaacccaca gcacgctgta ctggtcttta tcgagtccgg
     3781 gcatcagaac ccgctcgaag ctcgccatca gcgtttcacg ctgcccgggc ggtaagtcct
     3841 gttcggcaaa ggacagcacc ccggaggtgt actttttggc gtagggcgag gcgtcgataa
     3901 gctcccggac ttcctccggc ttcccctgaa gaacgctggc accgtcgcgc tggcggtctt
     3961 tccccagcag ataatcaacc gggccggcac cgcctccgcg tccccgggga tgaaacttaa
     4021 cgatcatcgt cagccccctt ttccagtacg gcatgccgca gccgctcgag tccggcatcg
     4081 atggccatca gcgcgg
//
