LOCUS       NZ_CP058174             4426 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00056 plasmid pRHBSTW-00056_3,
            complete sequence.
ACCESSION   NZ_CP058174
VERSION     NZ_CP058174.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148464
            Assembly: GCF_013629455.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00056
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.5730736939225x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013629455.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:50:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,080
            CDSs (total)                      :: 4,965
            Genes (coding)                    :: 4,847
            CDSs (with protein)               :: 4,847
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 38 of 118
            Pseudo Genes (incomplete)         :: 86 of 118
            Pseudo Genes (internal stop)      :: 21 of 118
            Pseudo Genes (multiple problems)  :: 22 of 118
            Pseudo Genes (short protein)      :: 1 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058174.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00056"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00056_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            420..743
                     /locus_tag="HV047_RS25185"
                     /old_locus_tag="HV047_25200"
     CDS             420..743
                     /locus_tag="HV047_RS25185"
                     /old_locus_tag="HV047_25200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_176689730.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            952..>1386
                     /locus_tag="HV047_RS25525"
                     /pseudo=""
     CDS             952..>1386
                     /locus_tag="HV047_RS25525"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1421..1912
                     /locus_tag="HV047_RS25195"
                     /old_locus_tag="HV047_25210"
     CDS             1421..1912
                     /locus_tag="HV047_RS25195"
                     /old_locus_tag="HV047_25210"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1919..2134
                     /locus_tag="HV047_RS25200"
                     /old_locus_tag="HV047_25215"
     CDS             1919..2134
                     /locus_tag="HV047_RS25200"
                     /old_locus_tag="HV047_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_137500696.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSYLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2822..3037)
                     /locus_tag="HV047_RS25205"
                     /old_locus_tag="HV047_25220"
     CDS             complement(2822..3037)
                     /locus_tag="HV047_RS25205"
                     /old_locus_tag="HV047_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015387220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_155026131.1"
                     /translation="MGFWETVGKIAKGAANALDEKAHELQATKMRLESKSSEELKRLIK
                     SDGFFGSASNTEKSMAMKILRERGDV"
                     /name="transposase"
     gene            3371..3832
                     /locus_tag="HV047_RS25210"
                     /old_locus_tag="HV047_25225"
     CDS             3371..3832
                     /locus_tag="HV047_RS25210"
                     /old_locus_tag="HV047_25225"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128348982.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FEMPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMVIRHYYVDCHPYYDRLPPEK
                     SDDFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(4088..4198)
                     /locus_tag="HV047_RS25530"
     CDS             complement(4088..4198)
                     /locus_tag="HV047_RS25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063858821.1"
                     /translation="MHEPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     Typing          134..307
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.714"
                     /coverage="100.0"
                     /evalue="1.2e-79"
     Typing          complement(2..161)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.789"
                     /coverage="98.0"
                     /evalue="2.52e-61"
     Typing          complement(3872..4379)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.742"
                     /coverage="99.0"
                     /evalue="1.31e-158"
     Typing          734..2226
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.8"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggaaaaac ggcttcgccg cggccttctc gtttccctgt taagtctctt cctcgcatct
       61 accaggaaat ttccgccccg ctcgtaagcc atttccgctc gccgcagccg aacgaccgag
      121 cgcagcgagt cagtgagcga ggaagcggaa tatatcctgt accacatatt ctgctgacgc
      181 accggtgccg ccttttttct cctgccacat gaagcacttc actgacaccc tcatccgtgc
      241 caacatagta agccagtata cactccgcta gcgcttcgtg actggttcag ggctgcgccc
      301 cgaaacccgc caaaagcctg acgcgcctgc ggcttgtcca accccgcgcc gaccgggaaa
      361 gaggttcccg cccgtcccgg cgttatcagg agaccggttt gtcagagaaa cgcagcaaaa
      421 tgctcacgat gtgggtgacc gaagatgagc accgccgtct gctggaacgc tgcgacggta
      481 aacagctcgc ggcctggatg cggcagacct gcctggacga aaagcctgcg cgcaccggaa
      541 agctcccctc actctcgccg gcgctgctgc gccagcttgc cggcatgggc aataacctga
      601 accagatagc ccgccgtgtt aacgccggcg gcggcaccgg ccatgacagg gtacagattg
      661 tggcggcgct gatggccatc gatgccgggc ttgagcggct gcggcacgcc gttcttgaga
      721 aaggaacgga cgatgatcgt taaatttcat ccacgcggtc gcggcggtgg cgccgggccg
      781 gtggactatc ttctgggtaa agaccggcag cgtgaaggtg ccagcgtcct gcaggggaag
      841 ccggaagaag tccgggagct gattgacgcg tcgccttacg tgaagaaata cacctccggc
      901 gtcctgtcgt ttgcggaggc cgatctgcct cccggccagc gggaaaaact gatggccagc
      961 ttcgagcggg ttctgatgcc cggacttgat aaagatcagt acagcattct gtgggtggag
     1021 cacgaggaca aggggcgtct ggagctgaac tttctgatcc ccaataccga gctgctgacc
     1081 ggcaggcggc tccagccgta ctacgaccgc gccgaccggc cgcgtatcga tgcctggcag
     1141 accatcgtga acggcagact ggggctgcac gacccgaacg caccggaaaa ccgccgggcg
     1201 ctggtcacgc cgtccggact gccgaaaacg aagcaggagg ccgcagaagc catcacgcgc
     1261 ggactgctga cccttgcttc gtccggggag ctgaaaaccc gtcaggacgt cactgaggcg
     1321 ctggaacggt caggttttga ggtggtgcgc accacgaaaa gcagtatcag cattgccgac
     1381 ccggacgggg ggcgaaacat ccgactgaag ggagccatct atgagcagtc ttttaacgct
     1441 ggcgaaggac ttagagcagc aatcgaaagc gcagaagcag agtaccggcg agatgctgaa
     1501 agccgcattc agcgagcacg agcagtctgt cagagcggaa ctgagcgcaa gcgcgaggag
     1561 aatcagcgac gccatcctcg cacacgagca gagtatgagc gaggcgatgg agaaaaaccg
     1621 gcggagcgta ctgcggactg ccgggcggac atggctcacc atcctgatgg tgtcagcgct
     1681 gctgatcggc acgagcggga gcatactgtg gtggcagggg cagcagataa ccgacaatta
     1741 cacgcatctt cgtcagcagg aagataccct ggcgaaaatg accgcccgga cgtggggagt
     1801 gcgctatcag gagagcagcg acggccggag gtttcttatc ctgccgccgg ggatgcaaac
     1861 cgaagccatc ccctacgacg ggacgacatg gatccgcctg aaacaggagt agcggaaaat
     1921 gacagagctg gaaaagcagt tgctgagcgc attagagcag ctacagcagg actactcgca
     1981 aaggctggac gaatgggaga gcgccttcgc ggaatggcgg agcatgtgtg ggcttatgca
     2041 acgggagaac gcggtgctga acgagcgcgt gtcgtacttg agcacgcagg tgctgagttt
     2101 aagcgagcag ctgcgccgct tgtccgggaa ctgaatgcca ccgagttccg gcagagggag
     2161 aatgagtatc agaaaacact ggagctggag cgccagccgc agaaaaccta ccgcgggccg
     2221 acactttaaa ccatccaggc agctgacaac ccgcacgaag aggggctctt acttctcccg
     2281 ctaaataggt tcgtgatcaa aaaggtcacg acttattcag gagcgggaaa tgaagctgtt
     2341 aaatcttatt gaatcattgc ttaactgcct gtatctggcg gcggagctgt gggactggct
     2401 ggagaagcat ttgtcggttt ttaaggcatt ggcgcggatt ttagtgtgac tttaacatcc
     2461 atgcatctgg ctgcatgact atgcaccagt gtaatattca gcggctgcgt ttctggtgtt
     2521 ttttcgttat gtctgtcgcc actttctcgc gtctggcacc ggaaacgcag ccaaccccgt
     2581 ttcagcggtg cgggcaatgg gacgttatgg taaataagtg ggcgtaaaaa agcccgggtt
     2641 acgggcttct ggacgctatg acttaccacg acattctctg caccaacttt gattcgttgc
     2701 accagaagga atggttctga atccgaatag agtttgcaac tcacctgcag tatgagcggt
     2761 ggttcgcttg cagttagggc atgtatgagg aagattcacc gtgcctacca tttttctatc
     2821 cttaaacgtc tccgcgttct ctgagaattt tcattgccat gcttttttct gtgttactgg
     2881 ctgagccgaa aaaaccatca gatttaatga ggcgctttag ttcttcactg cttttgcttt
     2941 ctaagcgcat tttggttgcc tggagttcat gagctttttc atccaaagcg ttagctgcgc
     3001 cttttgcaat tttaccgacc gtttcccaaa atcccattta accactcctt aatgcttatc
     3061 caattggact tgttgtgatc cgacgactcc gacatttagg gcaataatca ggcgtgccat
     3121 aaccagtacg actggtccac tcatgcttac aatccaaaca cttagcccgg tagattgtcg
     3181 gtgccattga atactccatt gattttaaaa atatttgtat agtgcactaa aattatcggc
     3241 agtttttctc ggagcttgag aggtagataa ccctaagctg atatgttgcc tttatgatgg
     3301 ttttcgtgtg ccaaggttag ctaggtaagg gatttactgt gggaagtgct ggttcgcgaa
     3361 gtgaagcgtt atggtaaata catattatgg ttatttatta ggagtaactc tgggagagtg
     3421 ggctatggga tcagatgttc aatggcagat agataatggt tatttggcgc aagttatggg
     3481 ggatttttcg acagagtcgc ctagctttga aatgccaatg tttgggcgat attttgatac
     3541 ctttcaagct gctatacatg ttgcaaaagc tttcccaggg agtacgttgc agccatcaga
     3601 gtacgaagat aaaactggga tggtaatacg tcattattat gtggattgtc atccgtatta
     3661 tgatcgactt cctcctgaaa aaagcgatga ttttcagtat gatgaatacc cttctgctat
     3721 tcatgtggct gcgtgtattg atcaagccag caaagaactc gacgaactat tgtttggtgg
     3781 tggatatggg gaaaatggtg aggggcatgc aagcccgata attttatatt agtgtgaggg
     3841 ggcggaggga tattatccaa tttgtaaaat gatcttcttg agatcgtttt gctctgcgcg
     3901 taatctcttg ctctgaaaac gaaaaaacca cctggggagg tggtttttcg aaggttcagt
     3961 aagttgggga acttctgaac cgtggtaaca gtcttgtgcg agacgtgtca ccaaatctgt
     4021 ccttccagtg tagccgtagt ccggccacca cttcaagaac tctcgataca tctctcgcac
     4081 atcctgctta ccagtggcta ccgccaatgg cgattttgtc gtgtctttcc gggttggact
     4141 caagttgata gttaccggaa tgggcgtggt agtcggactg aacggggggt tcgtgcatac
     4201 agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa
     4261 cgcggccata acagcggagt gacaccggta aaccgaaagg caggaacagg agagcgcacg
     4321 agggagccgc cagggggaaa cgcctggtat ctttaagtcc tgtcgggttt cgccaccact
     4381 gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcct
//
