LOCUS       NZ_CP058171             2495 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00059 plasmid pRHBSTW-00059_5,
            complete sequence.
ACCESSION   NZ_CP058171
VERSION     NZ_CP058171.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148465
            Assembly: GCF_013629235.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00059
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109.974717656083x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013629235.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:55:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,698
            CDSs (total)                      :: 4,582
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 51 of 119
            Pseudo Genes (incomplete)         :: 80 of 119
            Pseudo Genes (internal stop)      :: 20 of 119
            Pseudo Genes (multiple problems)  :: 29 of 119
            Pseudo Genes (short protein)      :: 1 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058171.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00059"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00059_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            1067..1285
                     /locus_tag="HV048_RS23310"
                     /old_locus_tag="HV048_23315"
     CDS             1067..1285
                     /locus_tag="HV048_RS23310"
                     /old_locus_tag="HV048_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(1576..1761)
                     /locus_tag="HV048_RS23315"
                     /old_locus_tag="HV048_23320"
     CDS             complement(1576..1761)
                     /locus_tag="HV048_RS23315"
                     /old_locus_tag="HV048_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2127..2354)
                     /locus_tag="HV048_RS23320"
                     /old_locus_tag="HV048_23325"
     CDS             complement(2127..2354)
                     /locus_tag="HV048_RS23320"
                     /old_locus_tag="HV048_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          1442..1529
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(275..803)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 gaggaggctt atatgagatg taatccggcc gatacatata ccaagatgat ttaagtattc
       61 ctctttgtga ggttggatgt ctaatttata aaggatcttc ttgagatcct ttttttccgc
      121 gcgtaatctc ttgccctgta aacgaaaaaa ccaccctggc aggtggtttt tcgaaggtta
      181 ggtaatcctg gcagatcccc taaccgtggt aacagtcttg tgcgagacat gtcaccaaat
      241 ttgtcctttc agtgtagcct cactaaggcc gccacttcaa gaactcttga gacatctctc
      301 gcacatcctg tttgccaatg gccgttgcca atggcgatta gtcgtgtctt tccgggttgg
      361 actcaagttg atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca
      421 tacagtccat cctggagcga actgccttcc cggaactgag tgtcaggcgt ggaatgagaa
      481 accgcggcca taacagcgga gtgacaccgg taaaccgaaa ggcaggaatg cgggggagca
      541 cgagggagcc accaggggga aacgcctggt atctttaagc cgcatcgggt ttcgccacca
      601 ctgatttgag cgtcagattc tgtgatgctt gtcagggggg cggagcctat ggaaaaacgg
      661 ctttggcgcg gccttatgct ttcttcgtta agtatcttcc tggcatcccc caggaaattt
      721 ctgatccatc cgtaagcccg tcccgctcgc cgcagccgaa cgaccgagcg gagcgagtca
      781 gtgagcgagg aagcggaata tattctgtat cacattttct cctgacgcgt tttctttcac
      841 tttctgcgcc tgtcttatgt ggcattaatg ctatgtgtta ctgccatgct acatcttaag
      901 ccagtataca ctccgctagt gctccgtgac tggtccggcg ctgcgcccgg aacccgcctg
      961 taccggttca gcagccgttc cggcctgact gcaatttttt ttttttcatc cctgcccgct
     1021 accctgtaaa cctttcttct gcgttgccgt taacagtagg taagccatgt ttggtttttc
     1081 ttccggagcc ctgatgactc ttagtgatga tgagcgacac ctgcttgtgt ccgttgtttc
     1141 ggtctggcta cggcgtgccg gaggggatgc cggagccatg atgctggatg cataccggca
     1201 gattttgtct gaaaccgaac cggccgtaag gactgtcatg ctcgaatttc ttgaatccgt
     1261 tcgcattcat tacatttcgt cctgagcatt ttctgtattt ctgtccatac ttaaacgagg
     1321 cggccgtcac ggacggtggt cgtcgttcca tgatgctgtt atggacgagt gagagtaatc
     1381 taatctaact tgattgagtt ccgggcgtgc cgcccggttt tttttgctgt ttcccggtgt
     1441 ccgttccggg acaggatgtg catttgtaat accgcgcacg cgccggcatc acaaataccc
     1501 tggtcagggc tgcgccccga caccccgcca tcgcctcacg gccgcgcact gaattgcgca
     1561 cccgttcacc gattttcacg cattttcctc gcctatctgt gcgttcagca tggcgtaaag
     1621 cgcttcggcc tgatcgtgca ggtgctcaca gcagccggca gcctcatcca gatccatctg
     1681 ctcgagccgc tcaaggagga gaagtgtctg tgcgcggata aatttggcca tattgagggc
     1741 ggcggattcc tgtctggtca ctggtgattt tccccgtctg gtgatgattt ttgcgtgaaa
     1801 agagcatggc gtcattctgg tgcatagtca tgcatgccgt taaaatttaa caggcgcgtt
     1861 tctgacgggt tccggggtgg tttgttgtgg tttttgtcat ggtttcgtca ggaacgcgct
     1921 gaacgcgtct caggcggtgc gggcaacgga tgttatggta aattcttggg ggcttgatta
     1981 taaaataccg cacttcctct tatgtcttgt gctttttaga caaccctgaa agcttttagc
     2041 tttggtgatg caatttgctg ggattttggc ggagaaccac aggtaaagaa aaaggccaca
     2101 ttagcggcct ttttcggaga agatgctcag tgcggaattt cggagacttc tttaacgtcg
     2161 gttttattga tctgctgttt gacaccattc gcgtctgtat agccaagcag gcctgagtct
     2221 gattctttag gtttgccgtc gctgacaata gtgcgaccgt cattggtgtg tatggcatag
     2281 cttgtgcgcg tgcagccggt gagggctgaa agcgcaactg ccgctgcgaa cactgaaaca
     2341 aaaatctttt tcaaagccag ctccttttat ccatatcagt tttggtcact aaccatctaa
     2401 ctataggtca ttttttatgt gaaaagaggc ttgatggtgg gctgtatatc gagttaggcg
     2461 cgaggtgcta tggtcaaagt gtggtgtcag gcggg
//
