LOCUS       NZ_CP058170             4990 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00059 plasmid pRHBSTW-00059_4,
            complete sequence.
ACCESSION   NZ_CP058170
VERSION     NZ_CP058170.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148465
            Assembly: GCF_013629235.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00059
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109.974717656083x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013629235.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:55:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,698
            CDSs (total)                      :: 4,582
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 51 of 119
            Pseudo Genes (incomplete)         :: 80 of 119
            Pseudo Genes (internal stop)      :: 20 of 119
            Pseudo Genes (multiple problems)  :: 29 of 119
            Pseudo Genes (short protein)      :: 1 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058170.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00059"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00059_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            357..560
                     /locus_tag="HV048_RS23270"
                     /old_locus_tag="HV048_23275"
     CDS             357..560
                     /locus_tag="HV048_RS23270"
                     /old_locus_tag="HV048_23275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017191052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071686691.1"
                     /translation="MAIYVGPHSKVGRNGGSFQECDINTTNHTVTRKYGRYCTIRDNEY
                     APPTSVPGRHWLQIFRTPDNTE"
                     /name="hypothetical protein"
     gene            695..922
                     /locus_tag="HV048_RS23275"
                     /old_locus_tag="HV048_23280"
     CDS             695..922
                     /locus_tag="HV048_RS23275"
                     /old_locus_tag="HV048_23280"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053504437.1"
                     /translation="MGYHYKELTVIELTAEEFELHKEGYSLELLTNMRNEEHTKYWEVC
                     PSCEGKMDPEENQGHEGYCATCWLDNPDWW"
                     /name="hypothetical protein"
     gene            complement(1405..1620)
                     /locus_tag="HV048_RS23280"
                     /old_locus_tag="HV048_23285"
     CDS             complement(1405..1620)
                     /locus_tag="HV048_RS23280"
                     /old_locus_tag="HV048_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_046275712.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWQSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1627..2118)
                     /locus_tag="HV048_RS23285"
                     /old_locus_tag="HV048_23290"
     CDS             complement(1627..2118)
                     /locus_tag="HV048_RS23285"
                     /old_locus_tag="HV048_23290"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_154819350.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2153..2857)
                     /locus_tag="HV048_RS23640"
                     /old_locus_tag="HV048_23295"
                     /pseudo=""
     CDS             complement(<2153..2857)
                     /locus_tag="HV048_RS23640"
                     /old_locus_tag="HV048_23295"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2796..3119)
                     /locus_tag="HV048_RS23295"
                     /old_locus_tag="HV048_23300"
     CDS             complement(2796..3119)
                     /locus_tag="HV048_RS23295"
                     /old_locus_tag="HV048_23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_180255778.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIMAVLMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3768..3899
                     /locus_tag="HV048_RS23645"
     CDS             3768..3899
                     /locus_tag="HV048_RS23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058645281.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPEKHDLTPLAAATGKGCTRDN"
                     /name="hypothetical protein"
     gene            4249..4557
                     /locus_tag="HV048_RS23300"
                     /old_locus_tag="HV048_23305"
     CDS             4249..4557
                     /locus_tag="HV048_RS23300"
                     /old_locus_tag="HV048_23305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003341209.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_012477691.1"
                     /translation="MKNSFELISTDPVKFNTWSIKSKLIIIIRKLMIDNKWSQSEAAEK
                     LGISQPRVSNMLNGKLDKFSVDTLLEMVFKMGYKLDMDFTPLNTESPLTMVVKKAMV"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(4573..4905)
                     /locus_tag="HV048_RS23305"
                     /old_locus_tag="HV048_23310"
     CDS             complement(4573..4905)
                     /locus_tag="HV048_RS23305"
                     /old_locus_tag="HV048_23310"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_045332894.1"
                     /translation="MSNDENEYTFKPVNAQAIKALKSLPEWVQAAFVHALDSVFYGVDP
                     GMSIAHLTVIGPGVVELRINGRPAYRCVYRSKAPGQVDLLHAFVKTCQGPDKKNMATTA
                     SRYKLL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(3233..3406)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          3373..3899
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.811"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1314..2806)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.8"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttatgagcat aaaaaaacca caccgcccta tatttaggaa agtgcgcgca tggttattgc
       61 tcgttgaacc aacaattcat tggcaatctc gccaacgatg ccatagtata taaattctta
      121 tatgtcatca attcttatta gtgcttcaaa cgtctgtttg aataaaaaaa aatcaatttg
      181 acttaggcgc agaagaccta acagttacat gtgttggttt tccttacatc aagatatgag
      241 gattttatcg acgcaatgtg gaaagccaac aaagcacact atgatattga taaatataga
      301 aattaataaa atctacttag atactggtaa tcaactatct gacgaaggag cttagaatgg
      361 ctatttatgt tggtcctcac agcaaagtag gtcgtaatgg tggttcattc caggaatgtg
      421 atatcaacac aacgaatcat acggtaaccc gaaaatatgg ccgctactgc acaatccgcg
      481 ataatgagta cgcccctcca accagcgttc caggccgcca ttggctgcaa attttccgca
      541 cgccagataa cacagagtaa ttcatcttct gcttagtaag cggtaatatc ccttgtataa
      601 accggatagg attatcattg taagatactg aaaacactga catttaatgc ttttcagtat
      661 cttagtattg agctgcataa agcgaggcaa gcgaatgggt taccattaca aggaattaac
      721 cgtcatcgaa ttaactgcgg aagagtttga gcttcataaa gagggatact ctttagagct
      781 tttaactaac atgagaaatg aagaacacac caagtattgg gaagtttgcc cttcctgcga
      841 aggaaaaatg gatccagaag aaaatcaagg gcatgaaggt tactgcgcca catgctggct
      901 cgataaccct gactggtggt agttaataat ttaccataac atccgttgcc cgcaccgctg
      961 aaacggggtt ggctgcgttt ccggtgccag acgcgagaaa gtggcgacag acataacgaa
     1021 aaaacaccag aaacgcagcc gctggatatt acactgatgc atagtcatgc agccagatgc
     1081 atgccgcgta aagtcacact aaaatcagcg cgaacgcctt aaaaacagga aagtgcttct
     1141 ccagccagtc ccagatctcc agcgccatgt acaggcagtt cagcaccgtc tcaatcacgt
     1201 taaacagtct cataatccct ccgttgtata cgctgtgatg tttccatcac gaccgtattt
     1261 agcgggaaat ttatgagccc ttcttcttgg ggtttgtcac gggccgtcag cccagcgttg
     1321 ggccacggta ggttttctgc ggctggtgct ccagctccag tgttttctga tactcatgct
     1381 ccctgtgccg gagctcgatg gcattcagct cccggacaag cggcgcagct gctcgcttaa
     1441 actcagcacc tgcgtgctca agtccgacac gcgctcgttc agcatcgcgt tctcccgttg
     1501 cataagacca cacatgctct gccattccgc gaaggcgctc tcccattcgt ccagcctttg
     1561 cgagtagtcc tgctgtagct gctctaatgc gctcagcaac tgcttttcca gctctgtcat
     1621 tttccgctac tcctgtttca ggcggatcca tgtcgtcccg tcgtaaggga tggcttcggt
     1681 ttgcatcccc ggcggcagga taagaaacct ccggccgtcg ctgctctcct gatagcgcac
     1741 tccccacgtc cgggcggtca ttttcgccag ggtatcttcc tgctgacgaa gatgcgtgta
     1801 attgtcggtt atctgctgcc cctgccacca cagaatgctc ccgctcgtgc cgatcagcag
     1861 cgctgacacc atcaggatgg tgagccatgt ccgcccggca gtccgcagta cgctccgccg
     1921 gtttttctcc atcgcctcgc tcatactctg ctcgtgggcg aggatggcgt cgctgattct
     1981 cctcgcgctt gcgctcagtt ccgctctgac agactgctcg tgctcgctga atgcggcttt
     2041 cagcatctcg ccggtactct tctgctgcgc tttcgattgc tgctctaagt ccttcgccag
     2101 cgttaaaaga ctgctcatag atggctccct tcagtcggat gtttcgcccc ccgtccgggt
     2161 cggcaatgct gatactgctt ttcgtggtgc gcaccacctc aaaacctgac cgttccagcg
     2221 cctcagtgat gtcctgacgg gttttcagct ccccggacga agcaaggttc agcagtccgc
     2281 gcgtgatgaa ttctgcggcc tcctgcttcg ttttcggcag tccggacggc gtgaccagcg
     2341 cccggcggtt ttccggtgcg ttcgggtcgt gcagccccag tctgccgttc acgatggtct
     2401 gccaggcatc gatacgcggc cggtcggcgc ggtcgtagta cggctggagc cgcctgccgg
     2461 tcagcagctc ggtattgggg atcagaaagt tcagctccag acgccccttg tccgcgtgct
     2521 ccacccacag aatgctgtac tgatctttat cgagtccggg catcagaacc cgctcgaagc
     2581 tggccatcag tttttcccgc tggccgggag gcagatcggc ctccgcaaac gacaggacgc
     2641 cggaggtgta tttcttcacg taaggcgacg cgtcaatcag ctcccggact tcttccggct
     2701 tcccctgcag aacgctggca ccttcacgct gccggtcttt acccagcaga tagtccaccg
     2761 gcccggcgcc accgccgcgg ccacgtggat gaaatttaac gatcatcgtc cgttcctttc
     2821 tcaagaacgg cgtgccgcag ccgctcaagg ccggcatcga tggccatcag caccgccata
     2881 atctgtaccc tgtcatggcc ggtgccgccg ccggcgttaa cacggcgggc tatctggttc
     2941 aggttattgc ccatgccggc aagctggcgc agcagcgccg gcgagattga ggggagcttt
     3001 ccggaacgtg ctggcttttc gtccaggcag gtctgccgca tccacgctgc cagctgcctg
     3061 ccgtcgcagc gctcgagcag ccgccggtgc tcgtcctccg tcacccacat ggtgagcatc
     3121 ttgttccgtt tctctgacaa accggcctcc tgataacgcc gggacgggcg ggaaaggctt
     3181 tcccggtcgg cgcggggttg gacaagccgc aggcgcgtca ggcttttggc gggtttcggg
     3241 gcgcagccct gaaccagtca cgtagcgcta gcggagtgta tactggctta ctatgttggc
     3301 acgaatgcgg aggtcagtga agtgcttcat gtggaaggag aaaaaatacg gcaccggcgc
     3361 gtcagcagaa tatgcgatac aggatatatt ccgcttcctc gctcactgac tcgctgcgct
     3421 cggtcgttcg gctgcggcga gcggaaatgg cttactggcg gggcggagat gtcctggaag
     3481 atgccaggaa gaaacttagc agggaagcaa taaggccgcg gcgaagccgt ttttccatag
     3541 gctccgcccc cctgacaagc atcacaaaat ctgacgctca aatcagtggt ggcgaaaccc
     3601 gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt
     3661 tcctgccttt cggtttaccg gtgtcactcc gctgttatgg ccgcgtttat ctcattccac
     3721 gcctgacact cggttccggg aaggcagttc gctccaagct ggactgtatg cacgaacccc
     3781 ccgttcagtc cgactgctgc gcctgttccg gtaactatcg acttgagtcc aacccggaaa
     3841 agcacgactt aacgccactg gcggcagcca ctggtaaagg gtgtacaaga gataactgat
     3901 gcagagttct tgaagtatca gtccaactac ggctaaactg gaaggacgaa ttaggtggca
     3961 gcggtcttgt acaccctgtt accacggttc ataaacttgc cggtttaatg aaccttcgaa
     4021 aaaccacctt gccgggtggt tttttcgttt atgggcaaag actactcttt gctggattga
     4081 tacgtcaaca caataagctt tacttaaaag atcaaaatgt ctttacactt tttcctcttg
     4141 aaagcaatat cattacttgt ataatgcccc gcccgtatat atatataagg agttccttgc
     4201 cggaactttt atcattgcga tacaggtaat ccgaagtgga gctgttctat gaaaaattca
     4261 tttgagctga tcagtacaga cccagtaaag ttcaatacat ggtcgatcaa gtcaaaactc
     4321 attatcataa ttagaaaact aatgattgat aacaaatggt cgcaatcaga agcggcagaa
     4381 aagttaggta tcagtcaacc aagagtaagc aatatgctga acggaaaact ggacaaattc
     4441 tccgtcgata cgctgttgga aatggttttc aagatggggt acaagctgga tatggacttc
     4501 actccattaa acacagagtc gcccctgact atggttgtaa aaaaggcgat ggtgtaacca
     4561 tcgccaattt gattagagca acttgtagcg cgaagctgtg gttgccatgt ttttcttatc
     4621 tggtccttgg cacgttttca caaacgcatg cagtaagtcc acctggccgg gtgccttact
     4681 gcggtataca cagcgataag ctgggcgacc attgattctc aactcaacaa cgccggggcc
     4741 gatgaccgtc aagtgtgcga tggacatccc cggatccacc ccgtagaaca ccgaatccaa
     4801 tgcgtgcacg aatgcagcct gaacccattc tgggagtgat tttaatgcct tgatagcctg
     4861 ggcattaaca ggtttgaacg tgtattcatt ttcgtcgttt gacataaaca tcctcatcta
     4921 ggttattgat ttttagcaac ttaactattg ttaaaacgtt tcgcatataa actccgattg
     4981 attgattgat
//
