LOCUS       NZ_CP058166             9923 bp    DNA     circular CON 01-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00070 plasmid pRHBSTW-00070_3,
            complete sequence.
ACCESSION   NZ_CP058166
VERSION     NZ_CP058166.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148471
            Assembly: GCF_013625795.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 9923)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T1-00070
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 115.355313570466x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013625795.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 04:12:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,751
            CDSs (total)                      :: 4,636
            Genes (coding)                    :: 4,549
            CDSs (with protein)               :: 4,549
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 38 of 87
            Pseudo Genes (incomplete)         :: 58 of 87
            Pseudo Genes (internal stop)      :: 10 of 87
            Pseudo Genes (multiple problems)  :: 18 of 87
            Pseudo Genes (short protein)      :: 1 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058166.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00070"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00070_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(9119..9923,1..32)
                     /locus_tag="HV054_RS23570"
                     /old_locus_tag="HV054_23580"
     CDS             join(9119..9923,1..32)
                     /locus_tag="HV054_RS23570"
                     /old_locus_tag="HV054_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(267..482)
                     /locus_tag="HV054_RS23575"
                     /old_locus_tag="HV054_23585"
     CDS             complement(267..482)
                     /locus_tag="HV054_RS23575"
                     /old_locus_tag="HV054_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_044704874.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(489..980)
                     /locus_tag="HV054_RS23580"
                     /old_locus_tag="HV054_23590"
     CDS             complement(489..980)
                     /locus_tag="HV054_RS23580"
                     /old_locus_tag="HV054_23590"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_063844470.1"
                     /translation="MSSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRAWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<1015..1719)
                     /locus_tag="HV054_RS23970"
                     /old_locus_tag="HV054_23595"
                     /pseudo=""
     CDS             complement(<1015..1719)
                     /locus_tag="HV054_RS23970"
                     /old_locus_tag="HV054_23595"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1658..1981)
                     /locus_tag="HV054_RS23590"
                     /old_locus_tag="HV054_23600"
     CDS             complement(1658..1981)
                     /locus_tag="HV054_RS23590"
                     /old_locus_tag="HV054_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_170949721.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLEQLRHAVLEKGTD
                     DDH"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2618..2776
                     /locus_tag="HV054_RS23975"
     CDS             2618..2776
                     /locus_tag="HV054_RS23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032670878.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHEKSLLAAAISNWRYTRDKL
                     RGLEVWA"
                     /name="hypothetical protein"
     gene            complement(3013..3381)
                     /locus_tag="HV054_RS23595"
                     /old_locus_tag="HV054_23605"
     CDS             complement(3013..3381)
                     /locus_tag="HV054_RS23595"
                     /old_locus_tag="HV054_23605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704866.1"
                     /translation="MALLISLVVVAGLYYQFWYKPHRAPILRIWGIAPDGQEFRLLLTE
                     HGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYLEDAHKNELLIELNESDLYPGGAIELQ
                     HNGGRKIHCEILKVRWSR"
                     /name="hypothetical protein"
     gene            3541..3774
                     /locus_tag="HV054_RS23600"
                     /old_locus_tag="HV054_23610"
     CDS             3541..3774
                     /locus_tag="HV054_RS23600"
                     /old_locus_tag="HV054_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461706.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_044704865.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDGRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="YgdI/YgdR family lipoprotein"
     gene            3796..4023
                     /locus_tag="HV054_RS23605"
                     /old_locus_tag="HV054_23615"
     CDS             3796..4023
                     /locus_tag="HV054_RS23605"
                     /old_locus_tag="HV054_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_000719360.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(4625..7510)
                     /locus_tag="HV054_RS23610"
                     /old_locus_tag="HV054_23620"
     CDS             complement(4625..7510)
                     /locus_tag="HV054_RS23610"
                     /old_locus_tag="HV054_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_001138082.1"
                     /translation="MPRRVTLTDRQKDALLRLPTSQTDLLKHYTLSDEDLGHIRLRRRA
                     HNRFGFALQLCVLRYPGRVLAPGELIPAEVIEFIGAQLGLGADDLVDYAAREETRHEHL
                     AELRGLYGFRTFSGRGASELKEWLFREAEMAVSNEDIARRFVAECRRTRTVLPATSTIE
                     RLCAAALVDAERRIETRIASRLPMSIREQLLALLEETADDRVTRFVWLRQFEPGSNSSS
                     ANRLLDRLEYLQRIDLPEDLLAGVPAHRVTRLRRQGERYYADGMRDLPEDRRLAILAVC
                     VSEWQAMLADAVVETHDRIVGRLYRASERICHAKVADEAGVVRDTLKSFAEIGGALVDA
                     QDDGQPLGDVIASGSGWDGLKTLVAMATRLTATMADDPLNHVLDGYHRFRRYAPRMLRL
                     LDLRAAPVALPLLEAVTALRTGLNDAAMTSFLRPSSKWHRHLRAQRAGDARLWEIAVLF
                     HLRDAFRSGDVWLTRSRRYGDLKHALVPAQSIAEGGRLAVPLRPEEWLADRQARLDMRL
                     RELGRAARAGTIPGGSIENGVLHIEKLEAAAPTGAEDLVLDLYKQIPPTRITDLLLEVD
                     AATGFTEAFTHLRTGAPCADRIGLMNVILAEGINLGLRKMADATNTHTFWELIRIGRWH
                     VEGEAYDRALAMVVEAQAALPMARFWGMGTSASSDGQFFVATEQGEAMNLVNAKYGNTP
                     GLKAYSHVSDQYAPFATQVIPATASEAPYILDGLLMNDAGRHIREQFTDTGGFTDHVFA
                     ACAILGYRFAPRIRDLPSKRLYAFNPSAAPAHLRALIGGKVNQAMIERNWPDILRIAAT
                     IAAGTVAPSQILRKLASYPRQNELATALREVGRVERTLFMIDWILDAELQRRAQIGLNK
                     GEAHHALKRAISFHRRGEIRDRSAEGQHYRIAGMNLLAAIIIFWNTMKLGEVVANQKRD
                     GKLLSPDLLAHVSPLGWEHINLTGEYRWPKP"
                     /name="Tn3 family transposase"
     gene            7636..8250
                     /locus_tag="HV054_RS23615"
                     /old_locus_tag="HV054_23625"
     CDS             7636..8250
                     /locus_tag="HV054_RS23615"
                     /old_locus_tag="HV054_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904895.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000904906.1"
                     /translation="MLIGYARVSKADGSQSLDLQHDALRAAGVERDNIYDDLASGGRDD
                     RPGLTACLKSLRDGDVLVVWKLDRLGRSLAHLVNTVKELSDRKIGLRVLTGKGAQIDTT
                     TASGRMVFGIFATLAEFERDLIRERTMAGLASARARGRKGGRKFALTKAQVRLAQAAMA
                     QRDTSVSDLCKELGIERVTLYRYVGPKGELRDHGKHVLGLT"
                     /name="recombinase family protein"
     gene            8316..9119
                     /gene="aph(3'')-Ib"
                     /locus_tag="HV054_RS23620"
                     /old_locus_tag="HV054_23630"
     ARG             8317..9116
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             9120..9922
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="96.4"
     Typing          complement(2088..2166)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.75"
                     /coverage="100.0"
                     /evalue="2.7400000000000003e-32"
     Typing          2242..2730
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.345"
                     /coverage="94.0"
                     /evalue="1.69e-147"
     Typing          complement(176..1668)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.8"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1665..1981)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="9.92e-61"
ORIGIN
        1 cgatcaagca ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg
       61 gaacacatca atcttaccgg agaatatcgt tggccaaagc cttagcgtag gatttcgccc
      121 tctcccgcaa acgacccctt cttggggttt atcacgggcc ggcatccgtc agcccagcgt
      181 cggtcctttg taggttttct gcggctggcg ctccagctcc agtgttttct gatactcatt
      241 ctccccctgc cggaactcgg tggcattcag ttcccggaca agcggcgcag ctgctcgctt
      301 aaactcagca cctgcgtgct caagtccgac acgcgctcgt tcagcaccgc gttctcccgt
      361 tgcataagcc cagacatctt ctgccattcc gcgaaggcgc tctcccattc gtccagcctt
      421 tgcgagtagt cctgatgtag ctgctctaat gcggtcagca actgcttttc cagctctgtc
      481 attttccgct actcctgttt caggcggatc caggtcgtcc cgtcgtaggg gatggcttcg
      541 gcttgcatcc ccggcggcag gataaggaac ctccggccgt cgctgctctc ctgatagcgc
      601 actccccacg tccgggcagt cattttcgcc agggtatctt cctgctgacg aagatgtgtg
      661 taattgtcgg ttatctgctg cccctgccac cacagaatgc tcccgctcgt ggcgatcagc
      721 agcgctgaca ccatcaggat ggtgagccat gccctcccgg ctgtcagcag cacgctccgc
      781 cggtttttct ccatcgcctc gctcatactc tgttcgtggg cgctgatggc gtcgctgatt
      841 ctcctcgcgc ttgcgctcag ttccgctctg acagactgct cgtgctcgct gaatgcggct
      901 ttcagcatct cgccggtact ctgctgctgc gctttcgatt tctgctccaa gtccttcgcc
      961 agcgttaaaa gactgctcat agatggctcc cttcagtcgg atgtttcgcc ccccgtccgg
     1021 gtcggcaatg ctgatgctgc ttttcgtgct gcgcaccacc tcaaaacctg cgctttccag
     1081 cgcctcagtg acgtcctgac gggttttcag ctccccggat gaggcaaggg caagcaggcc
     1141 gcgcgtaatc gcctgggcag cttcctgctt cgcttccggc agcgcggacg gcgtgaccag
     1201 cgcccgccgg ttttccggcg cgttcgggtc gtgcagcccc agccggccgt tgaccaccgt
     1261 ctgccaggca tcgatgcgcg gccggtcggc gcggtcgtag tacggctgga gccgcctgcc
     1321 ggtcagcagc tcggtattgg ggatcagaaa gttcagctcc agacgcccct tgtccgcgtg
     1381 ctccacccac agaatgctgt actgatcttt atcgaggccg ggcatcagaa cacgctcgaa
     1441 gctggccatc agtttttccc gctggccggg aggcagatct gcctccgcaa acgacagtac
     1501 gccggaggtg tatttcttca cgtaaggcga cacatcaatc agctcccgga cttcttccgg
     1561 cttgccctgc aggacggtgg cgccttcacg ctgccggtct ttacccagca aatagtccac
     1621 cggcccggcg ccaccgccgc gaccgcgcgg atgaaattta atgatcatcg tccgttcctt
     1681 tctcaagaac ggcgtgccgc agctgctcaa ggccggcatc gattgccatc agcgccgcca
     1741 caatctgcac cctgtcatgg ccggtaccgc cgccggcgtt aacgcggcgg gctatctggt
     1801 tcaggttgtt gcccatgccg gcaagctggc gcagcagcgc cggcgagatc gagggtagct
     1861 ttccggtacg cgcaggcttt tcatcaagac aggtctgccg catccacgcc gccagctgcc
     1921 tgccgtcaca gcgttcgagc agccgccggt gctcgtcctc cgtcacccac atggtgagca
     1981 tcttgttccg tttctctgac aaaccggtct cctgataacg ccgggacggg cgggaaagcc
     2041 tttcccggtc ggcgcggggt tggacaagcc gcaggcgcgt caggcttttg gcgggtttcg
     2101 gggcgcagcc ctgaaccagt cacgtagcgc tagcggagtg tatactggct tactatggtg
     2161 gcacgacggg aatcgggatg aaatagtcag tcataaaaac tgtgagatga ggctcaggtg
     2221 cgtaaggggc tgatgcctgt cccggtggat attccgcttc ctcgctcact gactcgctac
     2281 gctcggtcgt tcggctgcgg cgagcggtac ggactcacgg ataaagacgt gggggggaaa
     2341 tacgtgaatg caggagggga aaaaaggatg cggcgctgcc ggttttccat aggctccgcc
     2401 cccctgacaa gcatcacaga atctgacgct caaatcagtg gtggcgaaac ccgatgcggc
     2461 ttaaagatac caggcgtttc cccctgatgg ctccctcgtg ctcccccgca ttcctgcctt
     2521 tcggtttacc ggtgtcattc cgctgttatg gccgcggttt ctcattccac gcctgacact
     2581 cagttccgcg aaggcagttc gctccaggat tgactgtatg cacgaacccc ccgttcagtc
     2641 cgactgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacacgaaaa
     2701 atcgctactg gcagcagcca ttagtaactg gaggtatacg agagacaaat taagaggtct
     2761 tgaagtatgg gcctgagaga ggctacactg aaagaacatt tttggtttct gcggtctcgt
     2821 atacccagtt acctggttaa gcagttcccc aactgactta accttcgatc aaaccacctc
     2881 cccaggtggt tttttcgttt tcagagcaag agattacgac tgtaacgaca ggatcacaag
     2941 aagatcattt actcagagag actaactacc actatgtttg cctatcaaca tacttaggaa
     3001 acattacttg agttaacgac tccaccttac ttttagtatc tcgcaatgaa tcttccgacc
     3061 gccgttgtgt tgaagttcta ttgcgccacc ggggtataaa tcactttcgt ttaactcaat
     3121 taaaagctcg ttcttgtgag catcctctaa ataaaggtaa taacacccac cataaaggtt
     3181 ctgcgtatct ggcacggcaa caaaagattt ctttccgaac agtaaaacag cctctttttc
     3241 tccatgctct gttagcaata atcgaaattc ttgtccatca ggcgcgatcc cccatatacg
     3301 caagatcggc gctctatgtg gcttatacca aaattgatag taaagacctg ccactacaac
     3361 cagtgaaatt aataaggcca tttcaatcct caaaaacaag caacattcca aaatcgtttt
     3421 tcattcattt ttactgctgg gcatgatgcc caccttcaat cctcttttcg catgaaaaaa
     3481 cgacctatac ttagatggtt agtgaccata tccactttac ctttaagcag ggaacctatt
     3541 atgaataaaa aaacgctgtt ggcggccatg atggtcgcgt tggtgacctt tgccgctgct
     3601 ggatgttctt caaaccaaga aataaaaacc acagatggtc gcactatcgt tacagacggc
     3661 aaacccaaag ttgacagtga tacggggtta gtatcctaca aagatgccca tacaggccga
     3721 accgaacaga tcaaccgtga caaaattacc aacatgagtg aactagacaa ctgatacgga
     3781 taaaaggagc tggctttgaa aaagattttt gtttcagtgt tcgcagcggc agttgcgctt
     3841 tcagccctaa ccggctgcac gcgcacaagc tatgccatcc acaccaacga cgggcgtaca
     3901 attgtcagcg acggcaagcc tacagaatca gactcaggcc tgctgggcta taaagacgca
     3961 aatggtgtca aacagcagat caataaagcc gacgttaaag aagtctctga aattccgcat
     4021 tgaggcatcg ctctctccga aaagggccgc taatgcggcc cttttcttta tctgccccaa
     4081 caacaggcaa aaaaagcccc cgctgggggc caaatacctg cagtggctgg atagtactgc
     4141 attcctgatg aaggagacga ataagtatgg gccggatttt tgcgtctgca ggctcggtcg
     4201 ccacaatttt tccgtacccg ccaggcatca tgcttttacc ataacatccg ttgtctgcac
     4261 cgccggatcg gcactgacgg cgcagacggc acctggcacc cgaaaacccg gcttaagcaa
     4321 cgctacatcc tccgttaagc cgggagataa tttttacccg actgcatagc catgcagcca
     4381 ggtgaatgac ggcaggattc agcccaaaaa cagcgtgaag gccccgaaaa ccggcaaatg
     4441 cttttccagc cactcccaca gttctgccgc cagatgcagg cagttaagta cagcttcaat
     4501 aacattaagc agtttcataa ttccccctgg tataggccgt gatctttgca tcacgacgta
     4561 tttagcgggg aatttcgcag cccttcttct tggggtcgtt tgcgggaggg ggcggaatcc
     4621 tacgctaagg ctttggccag cgatattctc cggtgagatt gatgtgttcc catccgagcg
     4681 gcgaaacatg ggccaagaga tcgggcgata gcagctttcc atcgcgtttc tggtttgcaa
     4741 cgacctcgcc gagcttcatg gtgttccaga agatgatgat ggcggcgagc agattcatgc
     4801 cggcgatgcg gtaatgctgg ccttcggcgg aacggtcgcg gatttcaccg cggcggtgga
     4861 agctgattgc ccgcttcagc gcatgatgag cttcgccttt gttgagcccg atctgggcac
     4921 gccgttggag ttcggcatcc agaatccagt cgatcatgaa cagggtgcgc tcgacgcgac
     4981 cgacttcccg cagggctgtc gcgagctcgt tctgccgcgg ataggaggcg agtttccgca
     5041 gaatctggct tggcgcgacg gtcccggcag caatggtggc ggcgatgcgc aggatgtcgg
     5101 gccaattgcg ctcgatcatg gcttggttga cctttccgcc gatcaacgct cgcaggtgcg
     5161 ccggggcggc cgacggattg aacgcgtaga gccgtttgga tggcaggtcg cggatgcgcg
     5221 gagcgaaccg gtagccgaga atggcacatg cggcaaagac gtgatcggtg aagccgcccg
     5281 tgtcggtgaa ctgctcgcgg atatggcgtc cagcatcgtt catcagcagg ccatcgagga
     5341 tgtaaggcgc ttcgcttgcc gttgcaggaa tcacctgggt tgcgaacggc gcatattggt
     5401 cggagacgtg gctataggct ttcaggcccg gggtattgcc atatttcgcg ttgaccaggt
     5461 tcatggcctc accttgctct gtagcgacga agaactgtcc gtcgctcgaa gccgacgtgc
     5521 ccatgcccca gaaccgggcc atgggtaacg ctgcctgtgc ctcgaccacc atggccagcg
     5581 cccggtcata ggcttcgccc tcgacatgcc accgtccaat gcggatcaat tcccagaagg
     5641 tgtgggtgtt tgtcgcatcc gccattttgc gcaagccgag gttgatccct tccgccaaga
     5701 taacgttcat tagcccgatc cggtcagcgc agggtgctcc tgtgcgcaga tgggtgaacg
     5761 cttcggtgaa gccggtcgcc gcatccacct ccagcaggag atcggtgatg cgcgtgggcg
     5821 ggatctgctt gtagagatcg agcaccagat cttcggcgcc tgtcggcgcg gcggcttcga
     5881 gtttctcgat atgcagaacg ccgttttcaa tcgacccgcc cgggatcgtg cctgcgcgag
     5941 cggcacggcc aagctcgcgc aaccgcatgt cgaggcgagc ttgccggtct gccagccatt
     6001 cctccggccg caatggcaca gcgagacgac cgccttccgc gatggattgt gccggaacga
     6061 gtgcgtgttt cagatcgcca tagcgccggg acctagtaag ccagacatct ccggagcgga
     6121 acgcatcgcg cagatggaac agcaccgcga tctcccatag gcgagcgtcg ccagccctct
     6181 gggcccgaag gtggcgatgc catttcgagc tgggccgcaa gaagctggtc atcgcggcat
     6241 cgttcaaacc ggtacgaagg gccgtcaccg cttccagaag cggcagtgca acgggcgcag
     6301 ctcgcagatc gagcaggcgc aacatgcgtg gagcgtatcg gcggaagcgg tgataaccgt
     6361 cgagcacatg attgagcgga tcgtcggcca tggtggcggt cagcctggtt gccattgcaa
     6421 caagggtttt taagccgtcc caccctgacc cactcgcgat gacatcgccc agcggctggc
     6481 catcatcctg tgcatcgacc agggcgcccc cgatctcggc gaaggatttc agggtgtcac
     6541 gcaccacccc cgcttcgtct gcgacctttg catggcaaat acgctccgaa gcacggtaga
     6601 gacggccgac gatccggtcg tgggtttcga ccactgcgtc ggccaacatc gcctgccatt
     6661 ccgagacgca aacagccaag atcgcaagcc gcctgtcctc cgggagatcg cgcatgccgt
     6721 cggcataata ccgttcaccc tgcctgcgca gacgagtcac ccgatgggca ggaacgccgg
     6781 caagcagatc ctcggggaga tcgatgcgtt gcagatattc gagccggtcg agcagccggt
     6841 tggccgacga agagttcgag ccaggctcga actggcgcag ccacacaaaa cgggtcaccc
     6901 gatcatcagc cgtctcctcg agcaatgcca gcaactgttc tcggatcgac ataggcagcc
     6961 gactggcgat cctcgtctcg atgcgtcgct cggcatcgac gagagccgcg gcacaaagcc
     7021 gctcgatcgt ggatgtcgcg ggaaggacag tgcgggtgcg tcggcactcg gctacgaagc
     7081 gacgggcgat atcctcgttc gacaccgcca tctcggcttc tcggaacaac cattccttca
     7141 gctcgctcgc accacgtccg gagaaggtgc ggaagccgta gagcccccgt aactcggcaa
     7201 gatgctcgtg ccgtgtttcc tcgcgggcag catagtctac gagatcgtcg gcacccaggc
     7261 caagctgcgc tccgataaat tcgatgacct ctgcagggat cagttcgcct ggagccagca
     7321 cccggccggg atagcgcagg acacacaatt gcagggcgaa gccgaacctg ttgtgagcgc
     7381 gccgacgcag cctgatatgc ccaaggtctt catcactcag cgtatagtgc ttgagcaaat
     7441 ccgtctgtga agtcggcaag cgcaacagcg cgtctttctg ccgatcggtt agagtgacgc
     7501 gacgcggcat acatgttcct ttttcaaaat ctgatagcgt tcaagacgct ttgtttatga
     7561 agctggttga gatacatttc cagaggtcaa tgcaatcgtg gccgaagcgc cgcctcaaac
     7621 caacgtttgt gatacatgct gatcggatat gcccgcgtct ccaaagccga tggctcgcag
     7681 tctctcgacc tgcagcacga cgccttgcgc gccgcaggtg tcgaacggga caatatctat
     7741 gatgatcttg cttccggcgg tcgtgatgat cgccctggct tgactgcctg cctcaagtca
     7801 ttgcgtgacg gcgatgtgct ggtggtctgg aagctcgatc gcctcggacg atcgcttgcc
     7861 catctggtca acacggtgaa ggagctgtca gaccgcaaga tcggcctgcg ggttctgact
     7921 ggaaagggcg ctcagatcga caccacgact gcgtccggtc gcatggtgtt cggaatcttc
     7981 gccaccttgg ccgagttcga gcgggatctg atccgagagc gcaccatggc gggtctcgcc
     8041 tccgcgagag cgcgcggtcg caagggcgga cgaaaattcg cgctcaccaa agctcaggtg
     8101 cgtctcgcgc aagccgccat ggcccagcgc gatacttcag tttccgatct ctgcaaggaa
     8161 ctcggcatcg agcgcgtcac tctctaccga tatgtcggtc ccaaaggcga gctcagagac
     8221 catggaaagc atgttctcgg acttacgtag caactcgttt cttttcgcag gttgagccac
     8281 ctccgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     8341 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     8401 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     8461 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtga
     8521 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     8581 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     8641 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     8701 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     8761 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     8821 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga
     8881 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg
     8941 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg
     9001 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag
     9061 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat
     9121 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga
     9181 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa
     9241 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg
     9301 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga
     9361 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac
     9421 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc
     9481 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca
     9541 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag
     9601 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc
     9661 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc
     9721 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat
     9781 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga
     9841 ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca
     9901 acgcgatcta gctatcgcgg ccg
//
