LOCUS       NZ_CP058163             2511 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00083 plasmid pRHBSTW-00083_4,
            complete sequence.
ACCESSION   NZ_CP058163
VERSION     NZ_CP058163.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148476
            Assembly: GCF_013625575.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T1-00083
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 107.059809165582x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013625575.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 05:06:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,745
            CDSs (total)                      :: 4,630
            Genes (coding)                    :: 4,538
            CDSs (with protein)               :: 4,538
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 40 of 92
            Pseudo Genes (incomplete)         :: 49 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 19 of 92
            Pseudo Genes (short protein)      :: 1 of 92
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058163.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00083"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00083_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            342..485
                     /locus_tag="HV059_RS23795"
     CDS             342..485
                     /locus_tag="HV059_RS23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            913..1140
                     /locus_tag="HV059_RS23565"
                     /old_locus_tag="HV059_23575"
     CDS             913..1140
                     /locus_tag="HV059_RS23565"
                     /old_locus_tag="HV059_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1507..1692
                     /locus_tag="HV059_RS23570"
                     /old_locus_tag="HV059_23580"
     CDS             1507..1692
                     /locus_tag="HV059_RS23570"
                     /old_locus_tag="HV059_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(1740..1819)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          57..507
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.132"
                     /coverage="84.0"
                     /evalue="3.24e-165"
ORIGIN
        1 gagcggtacg ggcttacggg tggagcagaa atttcctagg ggatgccagc taggggatgc
       61 caggaagata cttagcgaag aaggcataag gccgcgccga agccgttttt ccataggctc
      121 cgcccccctg acgagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgatg
      181 cggcttaaag ataccaggcg tttccccctg atggctccct cgtgctcccc cgcattcctg
      241 cctttcggtt taccggtgtc actccgctgt tatggccgcg gtttctcatt ccacgcctga
      301 cactcggttc cgggaaggca gttcgctcca ggatggactg tatgcacgaa ccccccgttc
      361 agtccgactg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacacg
      421 taaaaacgcc actggcagcg gccactggta aggggtatac aagattactg gtgcagagtt
      481 cttgaagttg acgcctgact gcggctacac tagaaggaca aatttggtgg cagcggtctt
      541 gtacaccctg ttaccacggt tcagaaactt gccggtttgc tgaaccttcg aaaaaccacc
      601 ttgccgggtg gttttttcgt ttacagggca agagattacg cgcgaaaaaa aaggatctca
      661 agaagatcct tttataaatt agacatccaa cctcacaaag aggaatactt aaatcatctt
      721 ggtatatgta tcggtcggat tacatcagat ataagcccgc actgccccct ccccgccaga
      781 caccacactt taaccataac acctcgcacc taactcgata tgcagcccac catcaagcct
      841 cttttcacat aaaaaatgac ctatagttag atggttagtg accaaaactg atatggataa
      901 aaggagctgg ctttgaaaaa gatttttgtt tccgtgttcg cagcggcagt tgcgctttca
      961 gccctcaccg gctgcacgcg cacaagctat gccatacaca ccaatgacgg gcgcactatt
     1021 gtcagcgacg gcaaacctaa agaatcagac tcaggcctgc ttggctatac agacgcgaat
     1081 ggtgtcaaac agcagatcaa taaaaccgac gttaaagaag tctccgaaat tccgcactga
     1141 gcatcttctc cgaaaaaggc cgctaatgtg gcctttttct ttacctgggg ttctccgcca
     1201 aaatcccagc aaattgcacc accaaagcta aaagcttcca gggttgtcta aaaagcacaa
     1261 gacattagag gaagtgcggt ttttataatc aagcccccca agaatttacc ataacatccg
     1321 ttgcccgcac cgcctcagac gcactcagcg cttttctgac ggaaccatga ccaaaaacca
     1381 caacaaacca ccccggaacc cgccagaaac gctcctgtta aattttaacg gcatgcatga
     1441 ctatgcacca gaatgacggc atgctctttt cacgcaaaaa tcatcaccag acggggaaaa
     1501 tcaccagtga ccagacagga atccgccgcc ctcaacatgg ccaaatttat ccgctcacag
     1561 acactcctcc tcctcgagcg gctcgaacag atggatctgg atgaggctgc cggctgctgt
     1621 gagcacctgc acgatcaggc cgaagcactt tacaccatgc tgaacgcaca gacaggcgag
     1681 gaagatgcgt gaaaatcggt gaacgggtgc gcaattcagt gcgcggccgt gaggcgatgg
     1741 cggggtgtcg gggcgcagcc ctgacctggg tatttgtgat gccggcgcgt gcgcggtatt
     1801 acaaatacac atcctgtcct gagtggttcc ccgggaacag gcaaaaaaaa accggggagc
     1861 tgcccggaag gtaagtcaag ttagattaga ttactttcac tcatccataa cagcatcatg
     1921 caacgacgac catcgtccgt gacggccgcc tcgtttaagt atggacagaa atacagaaaa
     1981 tgctcaggac gaaatgtaat gaatgcgaac ggattcaaga aattcgagca tgaccgtcct
     2041 tacggccggt tcagtttcag acaaaatctg ccggtatgca tccagcatca tggctccggc
     2101 atccccccgg cacgccggag ccagcccgaa acaacagaca caaacaggtg tcgctcatca
     2161 tcaagagtca tcaggtctcc ggaagaaaaa ccaaacatgg cttacctcct gttaacggca
     2221 acgcagaaga aaggtttaca gggtagcggg caggaaaata aaaaaattgc agtcagcccg
     2281 gaacggctgc tgaaccggta caggcgggtt ccgggcgcag cgccggacca gtcactcagc
     2341 gctagcggag tgtatactgg ctaaaatgta acaccgcagt aacgcatagc ataactgcta
     2401 cataagacag gcgcagaaaa tgaaagaaaa cgcgtcagga gaaaatgtaa tacaggatat
     2461 attccgcttc ctcgctcact cactcgctgc gctcggtcgt tcggctgcgg c
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