LOCUS       NZ_CP058156             2511 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00105 plasmid pRHBSTW-00105_4,
            complete sequence.
ACCESSION   NZ_CP058156
VERSION     NZ_CP058156.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148488
            Assembly: GCF_013625265.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T1-00105
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 106.614656493829x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013625265.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:43:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,759
            CDSs (total)                      :: 4,644
            Genes (coding)                    :: 4,550
            CDSs (with protein)               :: 4,550
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 40 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 19 of 94
            Pseudo Genes (short protein)      :: 1 of 94
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058156.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00105"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00105_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2463..2511,1..95))
                     /locus_tag="HV071_RS23960"
     CDS             complement(join(2463..2511,1..95))
                     /locus_tag="HV071_RS23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            complement(1256..1441)
                     /locus_tag="HV071_RS23625"
                     /old_locus_tag="HV071_23640"
     CDS             complement(1256..1441)
                     /locus_tag="HV071_RS23625"
                     /old_locus_tag="HV071_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1808..2035)
                     /locus_tag="HV071_RS23630"
                     /old_locus_tag="HV071_23645"
     CDS             complement(1808..2035)
                     /locus_tag="HV071_RS23630"
                     /old_locus_tag="HV071_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          1130..1209
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          complement(2..498)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.4"
                     /coverage="92.0"
                     /evalue="2.8e-165"
     Typing          complement(2281..2474)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.935"
                     /coverage="89.0"
                     /evalue="1.52e-53"
ORIGIN
        1 gccagtggcg tttttacgtg tctttccggg ttggactcaa gacgatagtt accggataag
       61 gcgcagcagt cggactgaac ggggggttcg tgcatacagt ccatcctgga gcgaactgcc
      121 ttcccggaac cgagtgtcag gcgtggaatg agaaaccgcg gccataacag cggagtgaca
      181 ccggtaaacc gaaaggcagg aatgcggggg agcacgaggg agccatcagg gggaaacgcc
      241 tggtatcttt aagccgcatc gggtttcgcc accactgatt tgagcgtcag atttcgtgat
      301 gctcgtcagg ggggcggagc ctatggaaaa acggcttcgg cgcggcctta tgccttcttc
      361 gctaagtatc ttcctggcat cccctagctg gcatccccta ggaaatttct gctccacccg
      421 taagcccgta ccgctcgccg cagccgaacg accgagcgca gcgagtgagt gagcgaggaa
      481 gcggaatata tcctgtatta cattttctcc tgacgcgttt tctttcattt tctgcgcctg
      541 tcttatgtag cagttatgct atgcgttact gcggtgttac attttagcca gtatacactc
      601 cgctagcgct gagtgactgg tccggcgctg cgcccggaac ccgcctgtac cggttcagca
      661 gccgttccgg gctgactgca atttttttat tttcctgccc gctaccctgt aaacctttct
      721 tctgcgttgc cgttaacagg aggtaagcca tgtttggttt ttcttccgga gacctgatga
      781 ctcttgatga tgagcgacac ctgtttgtgt ctgttgtttc gggctggctc cggcgtgccg
      841 gggggatgcc ggagccatga tgctggatgc ataccggcag attttgtctg aaactgaacc
      901 ggccgtaagg acggtcatgc tcgaatttct tgaatccgtt cgcattcatt acatttcgtc
      961 ctgagcattt tctgtatttc tgtccatact taaacgaggc ggccgtcacg gacgatggtc
     1021 gtcgttgcat gatgctgtta tggatgagtg aaagtaatct aatctaactt gacttacctt
     1081 ccgggcagct ccccggtttt tttttgcctg ttcccgggga accactcagg acaggatgtg
     1141 tatttgtaat accgcgcacg cgccggcatc acaaataccc aggtcagggc tgcgccccga
     1201 caccccgcca tcgcctcacg gccgcgcact gaattgcgca cccgttcacc gattttcacg
     1261 catcttcctc gcctgtctgt gcgttcagca tggtgtaaag tgcttcggcc tgatcgtgca
     1321 ggtgctcaca gcagccggca gcctcatcca gatccatctg ttcgagccgc tcgaggagga
     1381 ggagtgtctg tgagcggata aatttggcca tgttgagggc ggcggattcc tgtctggtca
     1441 ctggtgattt tccccgtctg gtgatgattt ttgcgtgaaa agagcatgcc gtcattctgg
     1501 tgcatagtca tgcatgccgt taaaatttaa caggagcgtt tctggcgggt tccggggtgg
     1561 tttgttgtgg tttttggtca tggttccgtc agaaaagcgc tgagtgcgtc tgaggcggtg
     1621 cgggcaacgg atgttatggt aaattcttgg ggggcttgat tataaaaacc gcacttcctc
     1681 taatgtcttg tgctttttag acaaccctgg aagcttttag ctttggtggt gcaatttgct
     1741 gggattttgg cggagaaccc caggtaaaga aaaaggccac attagcggcc tttttcggag
     1801 aagatgctca gtgcggaatt tcggagactt ctttaacgtc ggttttattg atctgctgtt
     1861 tgacaccatt cgcgtctgta tagccaagca ggcctgagtc tgattcttta ggtttgccgt
     1921 cgctgacaat agtgcgcccg tcattggtgt gtatggcata gcttgtgcgc gtgcagccgg
     1981 tgagggctga aagcgcaact gccgctgcga acacggaaac aaaaatcttt ttcaaagcca
     2041 gctcctttta tccatatcag ttttggtcac taaccatcta actataggtc attttttatg
     2101 tgaaaagagg cttgatggtg ggctgcatat cgagttaggt gcgaggtgtt atggttaaag
     2161 tgtggtgtct ggcggggagg gggcagtgcg ggcttatatc tgatgtaatc cgaccgatac
     2221 atataccaag atgatttaag tattcctctt tgtgaggttg gatgtctaat ttataaaagg
     2281 atcttcttga gatccttttt tttcgcgcgt aatctcttgc cctgtaaacg aaaaaaccac
     2341 ccggcaaggt ggtttttcga aggttcagca aaccggcaag tttctgaacc gtggtaacag
     2401 ggtgtacaag accgctgcca ccaaatttgt ccttctagtg tagccgcagt caggcgtcaa
     2461 cttcaagaac tctgcaccag taatcttgta taccccttac cagtggccgc t
//
