LOCUS       NZ_CP058152             2495 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00114 plasmid pRHBSTW-00114_4,
            complete sequence.
ACCESSION   NZ_CP058152
VERSION     NZ_CP058152.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148493
            Assembly: GCF_013625115.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T1-00114
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95.5599346325099x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013625115.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 05:04:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,829
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,621
            CDSs (with protein)               :: 4,621
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 53 of 92
            Pseudo Genes (internal stop)      :: 24 of 92
            Pseudo Genes (multiple problems)  :: 16 of 92
            Pseudo Genes (short protein)      :: 1 of 92
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058152.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00114"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00114_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2409..2495,1..99))
                     /locus_tag="HV076_RS24085"
                     /old_locus_tag="HV076_24045"
     CDS             complement(join(2409..2495,1..99))
                     /locus_tag="HV076_RS24085"
                     /old_locus_tag="HV076_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(465..692)
                     /locus_tag="HV076_RS24090"
                     /old_locus_tag="HV076_24050"
     CDS             complement(465..692)
                     /locus_tag="HV076_RS24090"
                     /old_locus_tag="HV076_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1900..2118
                     /locus_tag="HV076_RS24095"
                     /old_locus_tag="HV076_24055"
     CDS             1900..2118
                     /locus_tag="HV076_RS24095"
                     /old_locus_tag="HV076_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          2275..2362
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1108..1636)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 ctcatccaga tccatctgct cgagccgctc aaggaggaga agtgtctgtg cgcggataaa
       61 tttggccata ttgagggcgg cggattcctg tctggtcact ggtgattttc cccgtctggt
      121 gatgattttt gcgtgaaaag agcatggcgt cattctggtg catagtcatg catgccgtta
      181 aaatttaaca ggcgcgtttc tgacgggttc cggggtggtt tgttgtggtt tttgtcatgg
      241 tttcgtcagg aacgcgctga acgcgtctca ggcggtgcgg gcaacggatg ttatggtaaa
      301 ttcttggggg cttgattata aaataccgca cttcctctta tgtcttgtgc tttttagaca
      361 accctgaaag cttttagctt tggtgatgca atttgctggg attttggcgg agaaccacag
      421 gtaaagaaaa aggccacatt agcggccttt ttcggagaag atgctcagtg cggaatttcg
      481 gagacttctt taacgtcggt tttattgatc tgctgtttga caccattcgc gtctgtatag
      541 ccaagcaggc ctgagtctga ttctttaggt ttgccgtcgc tgacaatagt gcgaccgtca
      601 ttggtgtgta tggcatagct tgtgcgcgtg cagccggtga gggctgaaag cgcaactgcc
      661 gctgcgaaca ctgaaacaaa aatctttttc aaagccagct ccttttatcc atatcagttt
      721 tggtcactaa ccatctaact ataggtcatt ttttatgtga aaagaggctt gatggtgggc
      781 tgtatatcga gttaggcgcg aggtgctatg gtcaaagtgt ggtgtcaggc ggggaggagg
      841 cttatatgag atgtaatccg gccgatacat ataccaagat gatttaagta ttcctctttg
      901 tgaggttgga tgtctaattt ataaaggatc ttcttgagat cctttttttc cgcgcgtaat
      961 ctcttgccct gtaaacgaaa aaaccaccct ggcaggtggt ttttcgaagg ttaggtaatc
     1021 ctggcagatc ccctaaccgt ggtaacagtc ttgtgcgaga catgtcacca aatttgtcct
     1081 ttcagtgtag cctcactaag gccgccactt caagaactct tgagacatct ctcgcacatc
     1141 ctgtttgcca atggccgttg ccaatggcga ttagtcgtgt ctttccgggt tggactcaag
     1201 ttgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc
     1261 catcctggag cgaactgcct tcccggaact gagtgtcagg cgtggaatga gaaaccgcgg
     1321 ccataacagc ggagtgacac cggtaaaccg aaaggcagga atgcggggga gcacgaggga
     1381 gccaccaggg ggaaacgcct ggtatcttta agccgcatcg ggtttcgcca ccactgattt
     1441 gagcgtcaga ttctgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttggc
     1501 gcggccttat gctttcttcg ttaagtatct tcctggcatc ccccaggaaa tttctgatcc
     1561 atccgtaagc ccgtcccgct cgccgcagcc gaacgaccga gcggagcgag tcagtgagcg
     1621 aggaagcgga atatattctg tatcacattt tctcctgacg cgttttcttt cactttctgc
     1681 gcctgtctta tgtggcatta atgctatgtg ttactgccat gctacatctt aagccagtat
     1741 acactccgct agtgctccgt gactggtccg gcgctgcgcc cggaacccgc ctgtaccggt
     1801 tcagcagccg ttccggcctg actgcaattt tttttttttc atccctgccc gctaccctgt
     1861 aaacctttct tctgcgttgc cgttaacagt aggtaagcca tgtttggttt ttcttccgga
     1921 gccctgatga ctcttagtga tgatgagcga cacctgcttg tgtccgttgt ttcggtctgg
     1981 ctacggcgtg ccggagggga tgccggagcc atgatgctgg atgcataccg gcagattttg
     2041 tctgaaaccg aaccggccgt aaggactgtc atgctcgaat ttcttgaatc cgttcgcatt
     2101 cattacattt cgtcctgagc attttctgta tttctgtcca tacttaaacg aggcggccgt
     2161 cacggacggt ggtcgtcgtt ccatgatgct gttatggacg agtgagagta atctaatcta
     2221 acttgattga gttccgggcg tgccgcccgg ttttttttgc tgtttcccgg tgtccgttcc
     2281 gggacaggat gtgcatttgt aataccgcgc acgcgccggc atcacaaata ccctggtcag
     2341 ggctgcgccc cgacaccccg ccatcgcctc acggccgcgc actgaattgc gcacccgttc
     2401 accgattttc acgcattttc ctcgcctatc tgtgcgttca gcatggcgta aagcgcttcg
     2461 gcctgatcgt gcaggtgctc acagcagccg gcagc
//
